MD12G1039700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013698
Physical Location & Seq
Reverse (-)
4306206 .. 4306937
732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1039700.v1.1.491

Sequence Viewer

Length: 732 bp
ATGATGGAGGCCGAAAGAGGCGTCTCGTTCCCGGTCGTCTTCTCCGACGGCGAGACCGAGACCGATATCGGCGACCTCGTCGTCTATCCGAAGCTTGATTTCTCGCGGTTCCTGTCGGCTCTGAGTCAAAAGATCGGAATCCTGCCGGGGCAGTTCACCGTCTTCCTCTCCTCGCCGGAGACCCGCCAGCGAATCCCCATCACCGGCAAGGTCAACTTCAGCGCGATTTCTCGCGAGAAGAACTGCTTCTTCCTCGTGGAGCTCAAGCGGTCGAGGCGCGAGCGGCGGCGGAAGAATCACAACTACCAGGTCCCCCACCACCACCAGGACTTCCGAGAAGATGAGTACCGTAACACGGCGTCGCTTGGAGCTCAAAATCCAATCAACAAGAATCTCCATCTCGAGAATGTTATGCTATTAAAGCGCGGCATGGAGATCGAAAACGTCGCCGGATTGGCCATGCCTTTTGCGGGTCGGGTCGAGTACGAGAATCGGGTCAGAGAGCTGCAGATTGAGAATGAGAGGTACTTGATGAAGATGGGCCTGGGAAGAGCCGATGGGCTCGGACTCGGGCTGGGCCGCCGGAGTGGGGCGACGGTATGCGAGGAGTGTTCGAGGGCGAACGAGATGGGGAGCGAAGTCGGGTTCCACTGGTGCGCTTACGACCCTGTGACTTTCGGGTTCAGGTCGCCGGCAGGTCCGATTTCCCGACCCGTTAAAGGGTCCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

27.53

Weight (kDa)

8.68

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7138 PF23596 7 - 86 6.1e-37 Domain of unknown function (DUF7138)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20260
malus_domestica MD12G1039700.v1.1 MD14G1038300.v1.1
prunus_persica Prupe.7G095200_v2.0.a1
pyrus_communis pycom03g00540 pycom12g03370 pycom14g03320
rosa_chinensis RchiOBHm_Chr3g0475851
rosa_laevigata RLG00000023807
rosa_multiflora Rmu_co8277165.1_g000001
rosa_roxburghii Rroxscaffold_6G00405790
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3BG232000 Rh3CG226600 Rh3DG227200 Rh4CG218200 Rh7BG133300
rosa_wichuraiana Rw3G018360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 686
AccBSI CCGCTC 1 cut(s) 283
AccII CGCG 5 cut(s) 106, 224, 234, 279, 426
AciI CCGC 9 cut(s) 106, 184, 268, 283, 286, 289, 426, 470, 580
AcoI YGGCCR 1 cut(s) 456
AcuI CTGAAG 1 cut(s) 202
AcyI GRCGYC 2 cut(s) 21, 359
AfaI GTAC 3 cut(s) 347, 485, 527
AfiI CCNNNNNNNGG 7 cut(s) 203, 325, 355, 470, 589, 719, 720
AjnI CCWGG 3 cut(s) 306, 324, 543
AluBI AGCT 4 cut(s) 94, 262, 371, 505
AluI AGCT 4 cut(s) 94, 262, 371, 505
Alw21I GWGCWC 2 cut(s) 264, 373
Alw26I GTCTC 4 cut(s) 28, 47, 53, 173
Ama87I CYCGRG 2 cut(s) 401, 569
AoxI GGCC 4 cut(s) 9, 456, 541, 577
ApeKI GCWGC 1 cut(s) 505
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 245
AspLEI GCGC 4 cut(s) 224, 279, 426, 659
AspS9I GGNCC 5 cut(s) 310, 541, 577, 698, 723
AsuC2I CCSGG 2 cut(s) 32, 147
AsuHPI GGTGA 2 cut(s) 148, 193
AvaI CYCGRG 2 cut(s) 401, 569
AvaII GGWCC 3 cut(s) 310, 698, 723
BalI TGGCCA 1 cut(s) 458
BanII GRGCYC 3 cut(s) 264, 373, 564
BauI CACGAG 1 cut(s) 254
BbsI GAAGAC 2 cut(s) 31, 154
Bbv12I GWGCWC 2 cut(s) 264, 373
BbvI GCAGC 1 cut(s) 492
BccI CCATC 5 cut(s) 206, 405, 532, 551, 622
BceAI ACGGC 2 cut(s) 64, 372
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 3 cut(s) 308, 326, 545
BcnI CCSGG 2 cut(s) 32, 147
BcoDI GTCTC 4 cut(s) 28, 47, 53, 173
BfaI CTAG 1 cut(s) 730
BfmI CTRYAG 1 cut(s) 506
BfuAI ACCTGC 1 cut(s) 686
BglI GCCNNNNNGGC 1 cut(s) 455
BisI GCNGC 5 cut(s) 284, 287, 427, 506, 580
BlsI GCNGC 5 cut(s) 285, 288, 428, 507, 581
Bme1390I CCNGG 5 cut(s) 32, 147, 308, 326, 545
Bme18I GGWCC 3 cut(s) 310, 698, 723
BmeT110I CYCGRG 2 cut(s) 401, 569
BmgT120I GGNCC 5 cut(s) 310, 541, 577, 698, 723
BmiI GGNNCC 4 cut(s) 110, 312, 647, 724
BmrFI CCNGG 5 cut(s) 32, 147, 308, 326, 545
BpiI GAAGAC 2 cut(s) 31, 154
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 32, 147
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 2 cut(s) 21, 359
BsaI GGTCTC 3 cut(s) 47, 53, 173
BsaJI CCNNGG 2 cut(s) 146, 544
Bsc4I CCNNNNNNNGG 7 cut(s) 203, 325, 355, 470, 589, 719, 720
Bse118I RCCGGY 2 cut(s) 203, 691
Bse1I ACTGG 1 cut(s) 656
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 3 cut(s) 308, 326, 545
BseDI CCNNGG 2 cut(s) 146, 544
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 7 cut(s) 203, 325, 355, 470, 589, 719, 720
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 656
BseRI GAGGAG 2 cut(s) 160, 620
BseXI GCAGC 1 cut(s) 492
BseYI CCCAGC 1 cut(s) 574
Bsh1236I CGCG 5 cut(s) 106, 224, 234, 279, 426
Bsh1285I CGRYCG 2 cut(s) 36, 272
BshFI GGCC 4 cut(s) 11, 458, 543, 579
BsiEI CGRYCG 2 cut(s) 36, 272
BsiHKAI GWGCWC 2 cut(s) 264, 373
BsiHKCI CYCGRG 2 cut(s) 401, 569
BsiSI CCGG 7 cut(s) 32, 146, 176, 204, 450, 583, 692
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 7 cut(s) 203, 325, 355, 470, 589, 719, 720
BsmAI GTCTC 4 cut(s) 28, 47, 53, 173
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 4 cut(s) 11, 458, 543, 579
Bso31I GGTCTC 3 cut(s) 47, 53, 173
BsoBI CYCGRG 2 cut(s) 401, 569
Bsp1286I GDGCHC 3 cut(s) 264, 373, 564
Bsp143I GATC 2 cut(s) 132, 435
Bsp68I TCGCGA 1 cut(s) 234
BspACI CCGC 9 cut(s) 106, 184, 268, 283, 286, 289, 426, 470, 580
BspANI GGCC 4 cut(s) 11, 458, 543, 579
BspCNI CTCAG 1 cut(s) 114
BspFNI CGCG 5 cut(s) 106, 224, 234, 279, 426
BspLI GGNNCC 4 cut(s) 110, 312, 647, 724
BspMAI CTGCAG 1 cut(s) 510
BspMI ACCTGC 1 cut(s) 686
BspQI GCTCTTC 1 cut(s) 544
BspTNI GGTCTC 3 cut(s) 47, 53, 173
BsrBI CCGCTC 1 cut(s) 283
BsrFI RCCGGY 2 cut(s) 203, 691
BsrI ACTGG 1 cut(s) 656
BssAI RCCGGY 2 cut(s) 203, 691
BssECI CCNNGG 2 cut(s) 146, 544
BssMI GATC 2 cut(s) 132, 435
BssNI GRCGYC 2 cut(s) 21, 359
BssSI CACGAG 1 cut(s) 254
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 3 cut(s) 308, 326, 545
Bst4CI ACNGT 3 cut(s) 160, 350, 598
Bst6I CTCTTC 1 cut(s) 544
BstACI GRCGYC 2 cut(s) 21, 359
BstC8I GCNNGC 3 cut(s) 188, 281, 693
BstDEI CTNAG 1 cut(s) 122
BstFNI CGCG 5 cut(s) 106, 224, 234, 279, 426
BstHHI GCGC 4 cut(s) 224, 279, 426, 659
BstKTI GATC 2 cut(s) 135, 438
BstMAI GTCTC 4 cut(s) 28, 47, 53, 173
BstMBI GATC 2 cut(s) 132, 435
BstMCI CGRYCG 2 cut(s) 36, 272
BstMWI GCNNNNNNNGC 4 cut(s) 274, 283, 421, 455
BstNI CCWGG 3 cut(s) 308, 326, 545
BstSCI CCNGG 5 cut(s) 30, 145, 306, 324, 543
BstSFI CTRYAG 1 cut(s) 506
BstUI CGCG 5 cut(s) 106, 224, 234, 279, 426
BstV1I GCAGC 1 cut(s) 492
BstV2I GAAGAC 2 cut(s) 31, 154
BsuRI GGCC 4 cut(s) 11, 458, 543, 579
BtsIMutI CAGTG 1 cut(s) 649
BtuMI TCGCGA 1 cut(s) 234
BveI ACCTGC 1 cut(s) 686
Cac8I GCNNGC 3 cut(s) 188, 281, 693
CfoI GCGC 4 cut(s) 224, 279, 426, 659
Cfr10I RCCGGY 2 cut(s) 203, 691
Cfr13I GGNCC 5 cut(s) 310, 541, 577, 698, 723
CseI GACGC 2 cut(s) 10, 348
CsiI ACCWGGT 1 cut(s) 306
Csp6I GTAC 3 cut(s) 346, 484, 526
CviAII CATG 2 cut(s) 430, 460
CviQI GTAC 3 cut(s) 346, 484, 526
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 134, 437
DpnII GATC 2 cut(s) 132, 435
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 1 cut(s) 544
EarI CTCTTC 1 cut(s) 544
EciI GGCGGA 1 cut(s) 304
Ecl136II GAGCTC 2 cut(s) 262, 371
Eco24I GRGCYC 3 cut(s) 264, 373, 564
Eco31I GGTCTC 3 cut(s) 47, 53, 173
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 3 cut(s) 310, 698, 723
Eco53kI GAGCTC 2 cut(s) 262, 371
Eco57I CTGAAG 1 cut(s) 202
Eco88I CYCGRG 2 cut(s) 401, 569
EcoICRI GAGCTC 2 cut(s) 262, 371
EcoO109I RGGNCCY 1 cut(s) 310
EcoRII CCWGG 3 cut(s) 306, 324, 543
EcoRV GATATC 1 cut(s) 67
EcoT38I GRGCYC 3 cut(s) 264, 373, 564
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 2 cut(s) 433, 463
FaiI YATR 4 cut(s) 413, 431, 461, 601
FalI AAGNNNNNCTT 4 cut(s) 200, 232, 230, 262
FaqI GGGAC 1 cut(s) 296
FatI CATG 2 cut(s) 429, 459
FauI CCCGC 2 cut(s) 191, 463
Fnu4HI GCNGC 5 cut(s) 284, 287, 427, 506, 580
FriOI GRGCYC 3 cut(s) 264, 373, 564
Fsp4HI GCNGC 5 cut(s) 284, 287, 427, 506, 580
FspBI CTAG 1 cut(s) 730
GlaI GCGC 4 cut(s) 223, 278, 425, 658
GluI GCNGC 5 cut(s) 284, 287, 427, 506, 580
GsaI CCCAGC 1 cut(s) 578
HaeIII GGCC 4 cut(s) 11, 458, 543, 579
HapII CCGG 7 cut(s) 32, 146, 176, 204, 450, 583, 692
HgaI GACGC 2 cut(s) 10, 348
HhaI GCGC 4 cut(s) 224, 279, 426, 659
Hin1I GRCGYC 2 cut(s) 21, 359
Hin1II CATG 2 cut(s) 433, 463
Hin6I GCGC 4 cut(s) 222, 277, 424, 657
HinP1I GCGC 4 cut(s) 222, 277, 424, 657
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 7 cut(s) 124, 138, 192, 295, 391, 490, 567
HpaII CCGG 7 cut(s) 32, 146, 176, 204, 450, 583, 692
HphI GGTGA 2 cut(s) 148, 193
Hpy166II GTNNAC 2 cut(s) 156, 214
Hpy188I TCNGA 9 cut(s) 46, 90, 123, 137, 335, 500, 566, 702, 727
Hpy188III TCNNGA 4 cut(s) 233, 401, 403, 708
Hpy8I GTNNAC 2 cut(s) 156, 214
Hpy99I CGWCG 5 cut(s) 50, 83, 364, 449, 598
HpyCH4III ACNGT 3 cut(s) 160, 350, 598
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 1 cut(s) 508
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 283, 421, 455
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 444
Hsp92I GRCGYC 2 cut(s) 21, 359
Hsp92II CATG 2 cut(s) 433, 463
HspAI GCGC 4 cut(s) 222, 277, 424, 657
KroI GCCGGC 1 cut(s) 691
KroNI GCCGGC 1 cut(s) 693
Kzo9I GATC 2 cut(s) 132, 435
LguI GCTCTTC 1 cut(s) 544
LmnI GCTCC 3 cut(s) 259, 368, 633
Lsp1109I GCAGC 1 cut(s) 492
MabI ACCWGGT 1 cut(s) 306
MaeI CTAG 1 cut(s) 730
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 350, 670
MalI GATC 2 cut(s) 134, 437
MbiI CCGCTC 1 cut(s) 283
MboI GATC 2 cut(s) 132, 435
MboII GAAGA 8 cut(s) 31, 154, 241, 250, 304, 350, 547, 561
MhlI GDGCHC 3 cut(s) 264, 373, 564
MlsI TGGCCA 1 cut(s) 458
MluNI TGGCCA 1 cut(s) 458
MlyI GAGTC 2 cut(s) 133, 561
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 9 cut(s) 11, 86, 176, 181, 263, 267, 516, 598, 609
Mox20I TGGCCA 1 cut(s) 458
MroNI GCCGGC 1 cut(s) 691
MroXI GAANNNNTTC 1 cut(s) 245
MscI TGGCCA 1 cut(s) 458
MseI TTAA 2 cut(s) 419, 717
Msp20I TGGCCA 1 cut(s) 458
MspI CCGG 7 cut(s) 32, 146, 176, 204, 450, 583, 692
MspR9I CCNGG 5 cut(s) 32, 147, 308, 326, 545
MvaI CCWGG 3 cut(s) 308, 326, 545
MvnI CGCG 5 cut(s) 106, 224, 234, 279, 426
MwoI GCNNNNNNNGC 4 cut(s) 274, 283, 421, 455
NaeI GCCGGC 1 cut(s) 693
NciI CCSGG 2 cut(s) 32, 147
NdeII GATC 2 cut(s) 132, 435
NgoMIV GCCGGC 1 cut(s) 691
NlaIII CATG 2 cut(s) 433, 463
NlaIV GGNNCC 4 cut(s) 110, 312, 647, 724
NmuCI GTSAC 1 cut(s) 670
NruI TCGCGA 1 cut(s) 234
PaeR7I CTCGAG 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 544
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 54, 75
PdiI GCCGGC 1 cut(s) 693
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 5 cut(s) 138, 192, 295, 391, 490
PflFI GACNNNGTC 1 cut(s) 77
PkrI GCNGC 5 cut(s) 285, 288, 428, 507, 581
PleI GAGTC 2 cut(s) 132, 561
PpsI GAGTC 2 cut(s) 132, 561
PpuMI RGGWCCY 1 cut(s) 310
Psp124BI GAGCTC 2 cut(s) 264, 373
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 3 cut(s) 306, 324, 543
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 3 cut(s) 306, 324, 543
PspN4I GGNNCC 4 cut(s) 110, 312, 647, 724
PspPI GGNCC 5 cut(s) 310, 541, 577, 698, 723
PspPPI RGGWCCY 1 cut(s) 310
PstI CTGCAG 1 cut(s) 510
PsyI GACNNNGTC 1 cut(s) 77
RruI TCGCGA 1 cut(s) 234
RsaI GTAC 3 cut(s) 347, 485, 527
RsaNI GTAC 3 cut(s) 346, 484, 526
SacI GAGCTC 2 cut(s) 264, 373
SapI GCTCTTC 1 cut(s) 544
SaqAI TTAA 2 cut(s) 419, 717
SatI GCNGC 5 cut(s) 284, 287, 427, 506, 580
Sau3AI GATC 2 cut(s) 132, 435
Sau96I GGNCC 5 cut(s) 310, 541, 577, 698, 723
SchI GAGTC 2 cut(s) 133, 561
ScrFI CCNGG 5 cut(s) 32, 147, 308, 326, 545
SduI GDGCHC 3 cut(s) 264, 373, 564
SexAI ACCWGGT 1 cut(s) 306
SfcI CTRYAG 1 cut(s) 506
Sfr274I CTCGAG 1 cut(s) 401
SinI GGWCC 3 cut(s) 310, 698, 723
SlaI CTCGAG 1 cut(s) 401
SmlI CTYRAG 2 cut(s) 263, 401
SmoI CTYRAG 2 cut(s) 263, 401
SsiI CCGC 9 cut(s) 106, 184, 268, 283, 286, 289, 426, 470, 580
SspMI CTAG 1 cut(s) 730
SstI GAGCTC 2 cut(s) 264, 373
StyD4I CCNGG 5 cut(s) 30, 145, 306, 324, 543
TaaI ACNGT 3 cut(s) 160, 350, 598
TaiI ACGT 1 cut(s) 447
TaqI TCGA 5 cut(s) 272, 402, 438, 480, 614
TaqII GACCGA 2 cut(s) 71, 77
TauI GCSGC 4 cut(s) 286, 289, 429, 582
TfiI GAWTC 5 cut(s) 138, 192, 295, 391, 490
Tru1I TTAA 2 cut(s) 419, 717
Tru9I TTAA 2 cut(s) 419, 717
TscAI CASTG 1 cut(s) 656
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 1 cut(s) 505
Tsp45I GTSAC 1 cut(s) 670
TspDTI ATGAA 1 cut(s) 548
TspRI CASTG 1 cut(s) 656
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 3 cut(s) 310, 698, 723
XhoI CTCGAG 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 245
XspI CTAG 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.