Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0006586

Physical Location & Seq

Genomic Coordinates Forward (+)

15114768 .. 15118934

Gene Structure 4167 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g21530.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGCTATGTGCTGACATGAGACGTGGTGACATCCAATCATTTCAATCTCATGAGCTCAGTATAATACATCTTCATAATAATGTAGCGAAATATTCTACAAGATTCAAAATGGCGCGTGTCGCAGTCCGTTGCAGTCGTTGGGCTCCGCGCTTCCTACCTTCCATTTCCAAAAACGAGCCGTTTCTCTCTTCCACCTTCTCCACCGCACCGGAAAATGCGAACCCGGGTCGACCCGGACCGCCTCCGATCCGGGTCGCGCTCACCGAGTCATCCGGGCGAGGCGTATTCGCGACCCGGAAAATCGAGACCGGTGAGCTCTTGCACACCGCCAAACCCATTCTGACTCACCCTTCTCTTTCCTCCCTCCACACCGTCTGCTACTTCTGCCTCCGAAAGCTCAGAACCGCCGACGCCTCTCAACCCCAAAGCGTTTCCTATTGCAGTCAGGAGTGTCAGCAACAAGCTCAGGGATTTCATGATATTGAGATGACAGCAGATTGGTCAGCTTACGATAACTATTGCCGGTCCAATGGTTTGAAATATCCACTACTTGTAAAGCGGTTGGCTTGTATGGTCATGTCAGGAGCTGCACATGCCAACCTTCTAGTCATACTCCAACCTGCTAGCTTAACTCCGGAGATGATATCTGAGATGGAAGAGGGGTTTGGCTTGCTAAGGAATGCCTTCATAAACTCAAATATCATGGGTGAACAGATGTCTTTCTTGACTAAACAATGGTACATCGGCATACTTGCACGGATTCGTATTAATGCATTTCGTATTGAGTTGGCTGGGGGATACAATGACCTCTTGTCATCATTAGCAGCCTCTATAGAAGCTGAAGCAGCTGTTGGGAATGCTGTTTATATGCTTCCATCCTTTTATAATCATGATTGTGATCCAAATGCCCACATCATATGGATAGAGAATTCAGATGCTAGATTGAAGGCCCTTCGTGATGTGGATGAAGGGGAAGAGCTCCGGATCTGCTACATCGATGCAAGTATGGATCATGATGCTCGGCAGAGTTTCCTGTCGCAAGGGTTTGGTTTCCGGTGCAATTGTCCCCGGTGTTTGTCTGGTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SET

Protein Analysis

363

Amino Acids

40.38

Weight (kDa)

6.82

Isoelectric Point (pI)

60.12

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06620 AT5G06620
fragaria_vesca	FvH4_6g21530 FvH4_6g21530 FvH4_6g21530 FvH4_6g21530 FvH4_6g21530
malus_domestica	MD12G1052100.v1.1
prunus_persica	Prupe.7G085000_v2.0.a1 Prupe.7G085000_v2.0.a1 Prupe.7G085000_v2.0.a1 Prupe.7G085000_v2.0.a1
pyrus_communis	pycom12g04590
rosa_chinensis	RchiOBHm_Chr3g0478741 RchiOBHm_Chr3g0478761
rosa_laevigata	RLG00000023628
rosa_multiflora	Rmu_sc0001519.1_g000008
rosa_roxburghii	Rroxscaffold_6G00403110
rosa_rugosa	Rorug03G0167600 Rorug03G0167700 Rorug03G0167800
rosa_samantha	Rh3AG219700 Rh3BG251500 Rh3CG248500 Rh3DG244700
rosa_wichuraiana	Rw3G019740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	885
Acc36I	ACCTGC	1 cut(s)	628
AccI	GTMKAC	1 cut(s)	229
AccII	CGCG	4 cut(s)	115, 148, 257, 290
AccIII	TCCGGA	2 cut(s)	634, 981
AciI	CCGC	6 cut(s)	146, 204, 239, 327, 405, 559
AclWI	GGATC	4 cut(s)	241, 893, 992, 1017
AcsI	RAATTY	1 cut(s)	928
AcuI	CTGAAG	1 cut(s)	861
AcyI	GRCGYC	1 cut(s)	411
AfaI	GTAC	1 cut(s)	740
AgeI	ACCGGT	1 cut(s)	308
AgsI	TTSAA	4 cut(s)	44, 106, 538, 946
AhdI	GACNNNNNGTC	1 cut(s)	811
AjiI	CACGTC	1 cut(s)	23
AjuI	GAANNNNNNNTTGG	4 cut(s)	648, 680, 834, 866
Alw21I	GWGCWC	3 cut(s)	57, 318, 981
Alw26I	GTCTC	2 cut(s)	13, 299
AlwI	GGATC	4 cut(s)	241, 893, 992, 1017
AlwNI	CAGNNNCTG	1 cut(s)	587
Ama87I	CYCGRG	1 cut(s)	223
Aor13HI	TCCGGA	2 cut(s)	634, 981
AoxI	GGCC	1 cut(s)	948
ApeKI	GCWGC	3 cut(s)	587, 824, 845
ApoI	RAATTY	1 cut(s)	928
AseI	ATTAAT	1 cut(s)	768
AsiGI	ACCGGT	1 cut(s)	308
Asp700I	GAANNNNTTC	1 cut(s)	683
AspLEI	GCGC	3 cut(s)	115, 150, 259
AspS9I	GGNCC	3 cut(s)	236, 525, 949
AsuC2I	CCSGG	7 cut(s)	224, 225, 234, 251, 274, 295, 1069
AsuHPI	GGTGA	5 cut(s)	38, 253, 323, 338, 719
AsuNHI	GCTAGC	1 cut(s)	623
AvaI	CYCGRG	1 cut(s)	223
AvaII	GGWCC	2 cut(s)	236, 525
BanII	GRGCYC	4 cut(s)	57, 145, 318, 981
Bbv12I	GWGCWC	3 cut(s)	57, 318, 981
BbvI	GCAGC	3 cut(s)	574, 836, 857
BccI	CCATC	2 cut(s)	646, 883
BceAI	ACGGC	1 cut(s)	163
BciVI	GTATCC	1 cut(s)	791
BcnI	CCSGG	7 cut(s)	224, 225, 234, 251, 274, 295, 1069
BcoDI	GTCTC	2 cut(s)	13, 299
BfaI	CTAG	3 cut(s)	605, 624, 939
BfmI	CTRYAG	1 cut(s)	831
BfuAI	ACCTGC	1 cut(s)	628
BfuI	GTATCC	1 cut(s)	791
BisI	GCNGC	3 cut(s)	588, 825, 846
BlsI	GCNGC	3 cut(s)	589, 826, 847
Bme1390I	CCNGG	7 cut(s)	224, 225, 234, 251, 274, 295, 1069
Bme18I	GGWCC	2 cut(s)	236, 525
BmeRI	GACNNNNNGTC	1 cut(s)	811
BmeT110I	CYCGRG	1 cut(s)	223
BmgBI	CACGTC	1 cut(s)	23
BmgT120I	GGNCC	3 cut(s)	236, 525, 949
BmiI	GGNNCC	1 cut(s)	144
BmrFI	CCNGG	7 cut(s)	224, 225, 234, 251, 274, 295, 1069
BmsI	GCATC	3 cut(s)	925, 988, 1006
BmtI	GCTAGC	1 cut(s)	627
Bpu10I	CCTNAGC	2 cut(s)	465, 674
BpuMI	CCSGG	7 cut(s)	224, 225, 234, 251, 274, 295, 1069
Bsa29I	ATCGAT	1 cut(s)	996
BsaBI	GATNNNNATC	1 cut(s)	897
BsaHI	GRCGYC	1 cut(s)	411
BsaI	GGTCTC	1 cut(s)	299
BsaJI	CCNNGG	2 cut(s)	223, 1067
BsaWI	WCCGGW	5 cut(s)	208, 308, 634, 981, 1053
Bse118I	RCCGGY	2 cut(s)	308, 522
Bse8I	GATNNNNATC	1 cut(s)	897
BseAI	TCCGGA	2 cut(s)	634, 981
BseCI	ATCGAT	1 cut(s)	996
BseDI	CCNNGG	2 cut(s)	223, 1067
BseGI	GGATG	4 cut(s)	30, 269, 875, 970
BseJI	GATNNNNATC	1 cut(s)	897
BseMII	CTCAG	4 cut(s)	70, 412, 479, 639
BseXI	GCAGC	3 cut(s)	574, 836, 857
BseYI	CCCAGC	1 cut(s)	791
BsgI	GTGCAG	1 cut(s)	573
Bsh1236I	CGCG	4 cut(s)	115, 148, 257, 290
BshFI	GGCC	1 cut(s)	950
BshTI	ACCGGT	1 cut(s)	308
BshVI	ATCGAT	1 cut(s)	996
BsiHKAI	GWGCWC	3 cut(s)	57, 318, 981
BsiHKCI	CYCGRG	1 cut(s)	223
BslFI	GGGAC	1 cut(s)	1050
BsmAI	GTCTC	2 cut(s)	13, 299
BsmBI	CGTCTC	1 cut(s)	13
BsmFI	GGGAC	1 cut(s)	1050
BsmI	GAATGC	2 cut(s)	685, 862
BsnI	GGCC	1 cut(s)	950
Bso31I	GGTCTC	1 cut(s)	299
BsoBI	CYCGRG	1 cut(s)	223
Bsp1286I	GDGCHC	4 cut(s)	57, 145, 318, 981
Bsp13I	TCCGGA	2 cut(s)	634, 981
Bsp143I	GATC	4 cut(s)	246, 898, 984, 1009
Bsp68I	TCGCGA	1 cut(s)	290
BspACI	CCGC	6 cut(s)	146, 204, 239, 327, 405, 559
BspANI	GGCC	1 cut(s)	950
BspCNI	CTCAG	4 cut(s)	69, 411, 478, 640
BspDI	ATCGAT	1 cut(s)	996
BspEI	TCCGGA	2 cut(s)	634, 981
BspFNI	CGCG	4 cut(s)	115, 148, 257, 290
BspHI	TCATGA	4 cut(s)	49, 475, 889, 1012
BspLI	GGNNCC	1 cut(s)	144
BspMI	ACCTGC	1 cut(s)	628
BspOI	GCTAGC	1 cut(s)	627
BspPI	GGATC	4 cut(s)	241, 893, 992, 1017
BspQI	GCTCTTC	1 cut(s)	969
BspTNI	GGTCTC	1 cut(s)	299
BsrFI	RCCGGY	2 cut(s)	308, 522
BssAI	RCCGGY	2 cut(s)	308, 522
BssECI	CCNNGG	2 cut(s)	223, 1067
BssMI	GATC	4 cut(s)	246, 898, 984, 1009
BssNI	GRCGYC	1 cut(s)	411
Bst4CI	ACNGT	1 cut(s)	373
Bst6I	CTCTTC	3 cut(s)	193, 651, 969
BstACI	GRCGYC	1 cut(s)	411
BstC8I	GCNNGC	2 cut(s)	625, 671
BstDEI	CTNAG	5 cut(s)	56, 398, 465, 648, 674
BstF5I	GGATG	4 cut(s)	30, 269, 875, 970
BstFNI	CGCG	4 cut(s)	115, 148, 257, 290
BstHHI	GCGC	3 cut(s)	115, 150, 259
BstKTI	GATC	4 cut(s)	249, 901, 987, 1012
BstMAI	GTCTC	2 cut(s)	13, 299
BstMBI	GATC	4 cut(s)	246, 898, 984, 1009
BstMWI	GCNNNNNNNGC	4 cut(s)	119, 384, 593, 845
BstNSI	RCATGY	1 cut(s)	596
BstSCI	CCNGG	7 cut(s)	222, 223, 232, 249, 272, 293, 1067
BstSFI	CTRYAG	1 cut(s)	831
BstUI	CGCG	4 cut(s)	115, 148, 257, 290
BstV1I	GCAGC	3 cut(s)	574, 836, 857
BstX2I	RGATCY	1 cut(s)	984
BstYI	RGATCY	1 cut(s)	984
Bsu15I	ATCGAT	1 cut(s)	996
BsuI	GTATCC	1 cut(s)	791
BsuRI	GGCC	1 cut(s)	950
BsuTUI	ATCGAT	1 cut(s)	996
BtrI	CACGTC	1 cut(s)	23
BtsCI	GGATG	4 cut(s)	30, 269, 875, 970
BtuMI	TCGCGA	1 cut(s)	290
BveI	ACCTGC	1 cut(s)	628
Cac8I	GCNNGC	2 cut(s)	625, 671
CaiI	CAGNNNCTG	1 cut(s)	587
CciI	TCATGA	4 cut(s)	49, 475, 889, 1012
CfoI	GCGC	3 cut(s)	115, 150, 259
Cfr10I	RCCGGY	2 cut(s)	308, 522
Cfr13I	GGNCC	3 cut(s)	236, 525, 949
Cfr9I	CCCGGG	1 cut(s)	223
ClaI	ATCGAT	1 cut(s)	996
CpoI	CGGWCCG	1 cut(s)	236
CseI	GACGC	1 cut(s)	419
Csp6I	GTAC	1 cut(s)	739
CspAI	ACCGGT	1 cut(s)	308
CspI	CGGWCCG	1 cut(s)	236
CviAII	CATG	8 cut(s)	16, 50, 476, 577, 593, 703, 890, 1013
CviQI	GTAC	1 cut(s)	739
DdeI	CTNAG	5 cut(s)	56, 398, 465, 648, 674
DpnI	GATC	4 cut(s)	248, 900, 986, 1011
DpnII	GATC	4 cut(s)	246, 898, 984, 1009
DriI	GACNNNNNGTC	1 cut(s)	811
Eam1104I	CTCTTC	3 cut(s)	193, 651, 969
Eam1105I	GACNNNNNGTC	1 cut(s)	811
EarI	CTCTTC	3 cut(s)	193, 651, 969
Ecl136II	GAGCTC	3 cut(s)	55, 316, 979
Eco24I	GRGCYC	4 cut(s)	57, 145, 318, 981
Eco31I	GGTCTC	1 cut(s)	299
Eco32I	GATATC	1 cut(s)	645
Eco47I	GGWCC	2 cut(s)	236, 525
Eco53kI	GAGCTC	3 cut(s)	55, 316, 979
Eco57I	CTGAAG	1 cut(s)	861
Eco88I	CYCGRG	1 cut(s)	223
EcoICRI	GAGCTC	3 cut(s)	55, 316, 979
EcoO109I	RGGNCCY	1 cut(s)	949
EcoRI	GAATTC	1 cut(s)	928
EcoRV	GATATC	1 cut(s)	645
EcoT22I	ATGCAT	1 cut(s)	775
EcoT38I	GRGCYC	4 cut(s)	57, 145, 318, 981
Esp3I	CGTCTC	1 cut(s)	13
FaeI	CATG	8 cut(s)	19, 53, 479, 580, 596, 706, 893, 1016
FaqI	GGGAC	1 cut(s)	1050
FatI	CATG	8 cut(s)	15, 49, 475, 576, 592, 702, 889, 1012
FauNDI	CATATG	1 cut(s)	917
FblI	GTMKAC	1 cut(s)	229
Fnu4HI	GCNGC	3 cut(s)	588, 825, 846
FokI	GGATG	4 cut(s)	17, 256, 862, 977
FriOI	GRGCYC	4 cut(s)	57, 145, 318, 981
Fsp4HI	GCNGC	3 cut(s)	588, 825, 846
FspBI	CTAG	3 cut(s)	605, 624, 939
GlaI	GCGC	3 cut(s)	114, 149, 258
GluI	GCNGC	3 cut(s)	588, 825, 846
GsaI	CCCAGC	1 cut(s)	795
HaeIII	GGCC	1 cut(s)	950
HgaI	GACGC	1 cut(s)	419
HhaI	GCGC	3 cut(s)	115, 150, 259
Hin1I	GRCGYC	1 cut(s)	411
Hin1II	CATG	8 cut(s)	19, 53, 479, 580, 596, 706, 893, 1016
Hin6I	GCGC	3 cut(s)	113, 148, 257
HinP1I	GCGC	3 cut(s)	113, 148, 257
HincII	GTYRAC	1 cut(s)	230
HindII	GTYRAC	1 cut(s)	230
HinfI	GANTC	4 cut(s)	102, 266, 343, 760
HphI	GGTGA	5 cut(s)	38, 253, 323, 338, 719
Hpy166II	GTNNAC	2 cut(s)	230, 710
Hpy188I	TCNGA	6 cut(s)	246, 342, 392, 401, 649, 934
Hpy8I	GTNNAC	2 cut(s)	230, 710
Hpy99I	CGWCG	1 cut(s)	413
HpyAV	CCTTC	8 cut(s)	168, 205, 360, 611, 694, 940, 962, 962
HpyCH4III	ACNGT	1 cut(s)	373
HpyCH4IV	ACGT	1 cut(s)	22
HpyCH4V	TGCA	8 cut(s)	132, 322, 441, 590, 755, 773, 1001, 1059
HpyF10VI	GCNNNNNNNGC	4 cut(s)	119, 384, 593, 845
HpyF3I	CTNAG	5 cut(s)	56, 398, 465, 648, 674
HpySE526I	ACGT	1 cut(s)	22
Hsp92I	GRCGYC	1 cut(s)	411
Hsp92II	CATG	8 cut(s)	19, 53, 479, 580, 596, 706, 893, 1016
HspAI	GCGC	3 cut(s)	113, 148, 257
Kpn2I	TCCGGA	2 cut(s)	634, 981
Kzo9I	GATC	4 cut(s)	246, 898, 984, 1009
LguI	GCTCTTC	1 cut(s)	969
LmnI	GCTCC	3 cut(s)	148, 584, 984
Lsp1109I	GCAGC	3 cut(s)	574, 836, 857
LweI	GCATC	3 cut(s)	925, 988, 1006
MaeI	CTAG	3 cut(s)	605, 624, 939
MaeII	ACGT	1 cut(s)	22
MaeIII	GTNAC	1 cut(s)	26
MalI	GATC	4 cut(s)	248, 900, 986, 1011
MboI	GATC	4 cut(s)	246, 898, 984, 1009
MboII	GAAGA	4 cut(s)	62, 180, 668, 986
MfeI	CAATTG	1 cut(s)	1060
MflI	RGATCY	1 cut(s)	984
MhlI	GDGCHC	4 cut(s)	57, 145, 318, 981
MluCI	AATT	2 cut(s)	928, 1060
MlyI	GAGTC	2 cut(s)	275, 337
MmeI	TCCRAC	1 cut(s)	640
MnlI	CCTC	9 cut(s)	252, 272, 370, 374, 398, 424, 652, 818, 838
Mph1103I	ATGCAT	1 cut(s)	775
MroI	TCCGGA	2 cut(s)	634, 981
MroXI	GAANNNNTTC	1 cut(s)	683
MseI	TTAA	3 cut(s)	629, 768, 1087
MslI	CAYNNNNRTG	2 cut(s)	78, 894
MspA1I	CMGCKG	1 cut(s)	848
MspR9I	CCNGG	7 cut(s)	224, 225, 234, 251, 274, 295, 1069
MunI	CAATTG	1 cut(s)	1060
Mva1269I	GAATGC	2 cut(s)	685, 862
MvnI	CGCG	4 cut(s)	115, 148, 257, 290
MwoI	GCNNNNNNNGC	4 cut(s)	119, 384, 593, 845
NciI	CCSGG	7 cut(s)	224, 225, 234, 251, 274, 295, 1069
NdeI	CATATG	1 cut(s)	917
NdeII	GATC	4 cut(s)	246, 898, 984, 1009
NheI	GCTAGC	1 cut(s)	623
NlaIII	CATG	8 cut(s)	19, 53, 479, 580, 596, 706, 893, 1016
NlaIV	GGNNCC	1 cut(s)	144
NmeAIII	GCCGAG	1 cut(s)	1000
NmuCI	GTSAC	1 cut(s)	26
NruI	TCGCGA	1 cut(s)	290
NsiI	ATGCAT	1 cut(s)	775
NspI	RCATGY	1 cut(s)	596
PagI	TCATGA	4 cut(s)	49, 475, 889, 1012
PciSI	GCTCTTC	1 cut(s)	969
PcsI	WCGNNNNNNNCGW	1 cut(s)	261
PctI	GAATGC	2 cut(s)	685, 862
PdmI	GAANNNNTTC	1 cut(s)	683
PfeI	GAWTC	2 cut(s)	102, 760
PinAI	ACCGGT	1 cut(s)	308
PkrI	GCNGC	3 cut(s)	589, 826, 847
PleI	GAGTC	2 cut(s)	274, 337
PpsI	GAGTC	2 cut(s)	274, 337
PshBI	ATTAAT	1 cut(s)	768
PsiI	TTATAA	1 cut(s)	885
Psp124BI	GAGCTC	3 cut(s)	57, 318, 981
PspFI	CCCAGC	1 cut(s)	791
PspN4I	GGNNCC	1 cut(s)	144
PspPI	GGNCC	3 cut(s)	236, 525, 949
PstNI	CAGNNNCTG	1 cut(s)	587
PsuI	RGATCY	1 cut(s)	984
PvuII	CAGCTG	1 cut(s)	848
RruI	TCGCGA	1 cut(s)	290
RsaI	GTAC	1 cut(s)	740
RsaNI	GTAC	1 cut(s)	739
RseI	CAYNNNNRTG	2 cut(s)	78, 894
Rsr2I	CGGWCCG	1 cut(s)	236
RsrII	CGGWCCG	1 cut(s)	236
SacI	GAGCTC	3 cut(s)	57, 318, 981
SalI	GTCGAC	1 cut(s)	228
SapI	GCTCTTC	1 cut(s)	969
SaqAI	TTAA	3 cut(s)	629, 768, 1087
SatI	GCNGC	3 cut(s)	588, 825, 846
Sau3AI	GATC	4 cut(s)	246, 898, 984, 1009
Sau96I	GGNCC	3 cut(s)	236, 525, 949
SchI	GAGTC	2 cut(s)	275, 337
ScrFI	CCNGG	7 cut(s)	224, 225, 234, 251, 274, 295, 1069
SduI	GDGCHC	4 cut(s)	57, 145, 318, 981
SfaNI	GCATC	3 cut(s)	925, 988, 1006
SfcI	CTRYAG	1 cut(s)	831
SinI	GGWCC	2 cut(s)	236, 525
SmaI	CCCGGG	1 cut(s)	225
SmiMI	CAYNNNNRTG	2 cut(s)	78, 894
Sse9I	AATT	2 cut(s)	928, 1060
SsiI	CCGC	6 cut(s)	146, 204, 239, 327, 405, 559
SspI	AATATT	1 cut(s)	92
SspMI	CTAG	3 cut(s)	605, 624, 939
SstI	GAGCTC	3 cut(s)	57, 318, 981
StyD4I	CCNGG	7 cut(s)	222, 223, 232, 249, 272, 293, 1067
TaaI	ACNGT	1 cut(s)	373
TaiI	ACGT	1 cut(s)	25
TaqI	TCGA	3 cut(s)	229, 303, 996
TasI	AATT	2 cut(s)	928, 1060
TfiI	GAWTC	2 cut(s)	102, 760
Tru1I	TTAA	3 cut(s)	629, 768, 1087
Tru9I	TTAA	3 cut(s)	629, 768, 1087
TseFI	GTSAC	1 cut(s)	26
TseI	GCWGC	3 cut(s)	587, 824, 845
Tsp45I	GTSAC	1 cut(s)	26
TspDTI	ATGAA	4 cut(s)	62, 464, 676, 981
TspGWI	ACGGA	2 cut(s)	116, 772
TspMI	CCCGGG	1 cut(s)	223
VpaK11BI	GGWCC	2 cut(s)	236, 525
VspI	ATTAAT	1 cut(s)	768
XapI	RAATTY	1 cut(s)	928
XceI	RCATGY	1 cut(s)	596
XmaI	CCCGGG	1 cut(s)	223
XmiI	GTMKAC	1 cut(s)	229
XmnI	GAANNNNTTC	1 cut(s)	683
XspI	CTAG	3 cut(s)	605, 624, 939
Zsp2I	ATGCAT	1 cut(s)	775

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.