Rroxscaffold_6G00403110

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006586
Physical Location & Seq
Reverse (-)
25558424 .. 25561993
3570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403110.1

Sequence Viewer

Length: 1008 bp
ATGCGAGTTGAGTCAAACATGGCGCGTGCGATAGTCCGTTATAGCCGTTGGGCTTCGCGCTTCTTAAAATCGAATTCCCAAAACGAGCCGTTTCTCTCTTCCGCTGCCGCCACCTTCTCCGCCGCACCGGAAAATGCGAATCCGGGTCGACCCGGACCGCCTCCGATCCGAGTTGCGCTCACCGAGTCATCCGGGCGAGGCGTCTTCGCGACCCGGAAAATCGAGACCGGTGAGCTCTTACACACCGCCAAACCCGTTCTATCTCACCCTTCTCTCTCCACCCTCCATGAGGTCTGCTACTTCTGCCTCCGAAAGCTCAAAACCACTGACGCCTCTCAACCCCAACGTGTTTCCTATTGCAGTGAGGAGTGTCGACAACAAGCTCAGGGATTTCATGATATTGAGATGACAGCAGATTGGTCAGCTTATGATGACTATTGCCGGTCTAATCGTTTGAAATATCCTCTACTTGTAAAGCGGTTGGCTTGTATGGTTATGTCAGGAGCTACACCTGCCAACCTTCTTGACATACTCCAGCCTGCCAGCTTATCTCCAGAGATGATTTCTGAGATGGAAGAGGGGTACGGCTTGCTAAGGAATGCCTACATAAACTCAAATATCACGGATGAACAGATGTCCTTTTTGACTAAACAATGGTACATTGGCGTACTTGCCCGGATTCGTATTAATGCATTTCGTATTGAGTTGGCTGGGGGATACGATGATCTCCTGTCATCATTAGCAGCCTCTATAGAATCTGAAGCTGCTGTTGGGAATGCCGTTTATATGCTTCCATCCTTTTATAATCATGATTGTGATCCAAATGCCCACATTATATGGATCGAGAACGCAGATGTGAGATTGAAGGCCCTTCGTGATGTGGATGAAGGGGAAGAGCTCCGGATCTGCTATATTGATGCAAGTATGGATCATGATGCTCGGCAGAGTTTCCTGTCACAAGGGTTTGGTTTTCAGTGCAATTGTCCCCGGTGTTTGTCTGGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

335

Amino Acids

37.4

Weight (kDa)

5.59

Isoelectric Point (pI)

59.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 65 - 304 9e-13 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 804
AarI CACCTGC 1 cut(s) 520
Acc36I ACCTGC 1 cut(s) 520
AccI GTMKAC 2 cut(s) 148, 373
AccII CGCG 3 cut(s) 25, 58, 209
AccIII TCCGGA 1 cut(s) 900
AciI CCGC 7 cut(s) 102, 108, 120, 123, 158, 246, 478
AclWI GGATC 5 cut(s) 160, 812, 848, 911, 936
AcsI RAATTY 1 cut(s) 73
AcuI CTGAAG 1 cut(s) 780
AcyI GRCGYC 2 cut(s) 201, 330
AfaI GTAC 3 cut(s) 584, 659, 669
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 1 cut(s) 346
AgeI ACCGGT 1 cut(s) 227
AgsI TTSAA 2 cut(s) 457, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AluBI AGCT 8 cut(s) 235, 316, 383, 425, 506, 546, 764, 898
AluI AGCT 8 cut(s) 235, 316, 383, 425, 506, 546, 764, 898
Alw21I GWGCWC 2 cut(s) 237, 900
Alw26I GTCTC 1 cut(s) 218
AlwI GGATC 5 cut(s) 160, 812, 848, 911, 936
Aor13HI TCCGGA 1 cut(s) 900
AoxI GGCC 1 cut(s) 867
ApeKI GCWGC 3 cut(s) 104, 743, 764
ApoI RAATTY 1 cut(s) 73
AseI ATTAAT 1 cut(s) 687
AsiGI ACCGGT 1 cut(s) 227
AspLEI GCGC 3 cut(s) 25, 60, 178
AspS9I GGNCC 2 cut(s) 155, 868
AsuC2I CCSGG 6 cut(s) 144, 153, 193, 214, 676, 988
AsuHPI GGTGA 3 cut(s) 172, 242, 257
AvaII GGWCC 1 cut(s) 155
BanII GRGCYC 2 cut(s) 237, 900
BbsI GAAGAC 1 cut(s) 196
Bbv12I GWGCWC 2 cut(s) 237, 900
BbvI GCAGC 3 cut(s) 91, 751, 755
BccI CCATC 2 cut(s) 565, 802
BceAI ACGGC 4 cut(s) 30, 73, 601, 764
BciVI GTATCC 1 cut(s) 710
BcnI CCSGG 6 cut(s) 144, 153, 193, 214, 676, 988
BcoDI GTCTC 1 cut(s) 218
BfmI CTRYAG 1 cut(s) 750
BfuAI ACCTGC 1 cut(s) 520
BfuI GTATCC 1 cut(s) 710
BisI GCNGC 5 cut(s) 105, 108, 123, 744, 765
BlsI GCNGC 5 cut(s) 106, 109, 124, 745, 766
Bme1390I CCNGG 6 cut(s) 144, 153, 193, 214, 676, 988
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 2 cut(s) 155, 868
BmrFI CCNGG 6 cut(s) 144, 153, 193, 214, 676, 988
BmsI GCATC 2 cut(s) 907, 925
BpiI GAAGAC 1 cut(s) 196
BplI GAGNNNNNCTC 2 cut(s) 162, 194
BpmI CTGGAG 2 cut(s) 518, 537
Bpu10I CCTNAGC 2 cut(s) 384, 593
BpuMI CCSGG 6 cut(s) 144, 153, 193, 214, 676, 988
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 2 cut(s) 201, 330
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 986
BsaWI WCCGGW 3 cut(s) 127, 227, 900
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse118I RCCGGY 2 cut(s) 227, 441
Bse8I GATNNNNATC 1 cut(s) 816
BseAI TCCGGA 1 cut(s) 900
BseDI CCNNGG 1 cut(s) 986
BseGI GGATG 4 cut(s) 188, 631, 794, 889
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMII CTCAG 2 cut(s) 398, 558
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 3 cut(s) 91, 751, 755
BseYI CCCAGC 1 cut(s) 710
Bsh1236I CGCG 3 cut(s) 25, 58, 209
BshFI GGCC 1 cut(s) 869
BshTI ACCGGT 1 cut(s) 227
BsiHKAI GWGCWC 2 cut(s) 237, 900
BslFI GGGAC 1 cut(s) 969
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 969
BsmI GAATGC 2 cut(s) 604, 781
BsnI GGCC 1 cut(s) 869
Bso31I GGTCTC 1 cut(s) 218
Bsp1286I GDGCHC 2 cut(s) 237, 900
Bsp13I TCCGGA 1 cut(s) 900
Bsp143I GATC 6 cut(s) 165, 724, 817, 840, 903, 928
Bsp68I TCGCGA 1 cut(s) 209
BspACI CCGC 7 cut(s) 102, 108, 120, 123, 158, 246, 478
BspANI GGCC 1 cut(s) 869
BspCNI CTCAG 2 cut(s) 397, 559
BspEI TCCGGA 1 cut(s) 900
BspFNI CGCG 3 cut(s) 25, 58, 209
BspHI TCATGA 3 cut(s) 394, 808, 931
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 5 cut(s) 160, 812, 848, 911, 936
BspQI GCTCTTC 1 cut(s) 888
BspTNI GGTCTC 1 cut(s) 218
BsrFI RCCGGY 2 cut(s) 227, 441
BssAI RCCGGY 2 cut(s) 227, 441
BssECI CCNNGG 1 cut(s) 986
BssMI GATC 6 cut(s) 165, 724, 817, 840, 903, 928
BssNI GRCGYC 2 cut(s) 201, 330
Bst6I CTCTTC 3 cut(s) 103, 570, 888
BstACI GRCGYC 2 cut(s) 201, 330
BstC8I GCNNGC 4 cut(s) 27, 540, 544, 590
BstDEI CTNAG 3 cut(s) 384, 567, 593
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 4 cut(s) 188, 631, 794, 889
BstFNI CGCG 3 cut(s) 25, 58, 209
BstHHI GCGC 3 cut(s) 25, 60, 178
BstKTI GATC 6 cut(s) 168, 727, 820, 843, 906, 931
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 6 cut(s) 165, 724, 817, 840, 903, 928
BstMWI GCNNNNNNNGC 2 cut(s) 303, 512
BstSCI CCNGG 6 cut(s) 142, 151, 191, 212, 674, 986
BstSFI CTRYAG 1 cut(s) 750
BstUI CGCG 3 cut(s) 25, 58, 209
BstV1I GCAGC 3 cut(s) 91, 751, 755
BstV2I GAAGAC 1 cut(s) 196
BstX2I RGATCY 1 cut(s) 903
BstYI RGATCY 1 cut(s) 903
BsuI GTATCC 1 cut(s) 710
BsuRI GGCC 1 cut(s) 869
BtsCI GGATG 4 cut(s) 188, 631, 794, 889
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 3 cut(s) 324, 367, 980
BtuMI TCGCGA 1 cut(s) 209
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 4 cut(s) 27, 540, 544, 590
CciI TCATGA 3 cut(s) 394, 808, 931
CfoI GCGC 3 cut(s) 25, 60, 178
Cfr10I RCCGGY 2 cut(s) 227, 441
Cfr13I GGNCC 2 cut(s) 155, 868
CpoI CGGWCCG 1 cut(s) 155
CseI GACGC 2 cut(s) 190, 338
Csp6I GTAC 3 cut(s) 583, 658, 668
CspAI ACCGGT 1 cut(s) 227
CspI CGGWCCG 1 cut(s) 155
CviAII CATG 5 cut(s) 19, 287, 395, 809, 932
CviQI GTAC 3 cut(s) 583, 658, 668
DdeI CTNAG 3 cut(s) 384, 567, 593
DpnI GATC 6 cut(s) 167, 726, 819, 842, 905, 930
DpnII GATC 6 cut(s) 165, 724, 817, 840, 903, 928
Eam1104I CTCTTC 3 cut(s) 103, 570, 888
EarI CTCTTC 3 cut(s) 103, 570, 888
EciI GGCGGA 1 cut(s) 109
Ecl136II GAGCTC 2 cut(s) 235, 898
Eco24I GRGCYC 2 cut(s) 237, 900
Eco31I GGTCTC 1 cut(s) 218
Eco47I GGWCC 1 cut(s) 155
Eco53kI GAGCTC 2 cut(s) 235, 898
Eco57I CTGAAG 1 cut(s) 780
EcoICRI GAGCTC 2 cut(s) 235, 898
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 868
EcoRI GAATTC 1 cut(s) 73
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 2 cut(s) 237, 900
FaeI CATG 5 cut(s) 22, 290, 398, 812, 935
FaqI GGGAC 1 cut(s) 969
FatI CATG 5 cut(s) 18, 286, 394, 808, 931
FblI GTMKAC 2 cut(s) 148, 373
Fnu4HI GCNGC 5 cut(s) 105, 108, 123, 744, 765
FokI GGATG 4 cut(s) 175, 638, 781, 896
FriOI GRGCYC 2 cut(s) 237, 900
Fsp4HI GCNGC 5 cut(s) 105, 108, 123, 744, 765
GlaI GCGC 3 cut(s) 24, 59, 177
GluI GCNGC 5 cut(s) 105, 108, 123, 744, 765
GsaI CCCAGC 1 cut(s) 714
GsuI CTGGAG 2 cut(s) 518, 537
HaeIII GGCC 1 cut(s) 869
HgaI GACGC 2 cut(s) 190, 338
HhaI GCGC 3 cut(s) 25, 60, 178
Hin1I GRCGYC 2 cut(s) 201, 330
Hin1II CATG 5 cut(s) 22, 290, 398, 812, 935
Hin6I GCGC 3 cut(s) 23, 58, 176
HinP1I GCGC 3 cut(s) 23, 58, 176
HincII GTYRAC 2 cut(s) 149, 374
HindII GTYRAC 2 cut(s) 149, 374
HinfI GANTC 5 cut(s) 11, 139, 185, 679, 755
HphI GGTGA 3 cut(s) 172, 242, 257
Hpy166II GTNNAC 2 cut(s) 149, 374
Hpy188I TCNGA 5 cut(s) 165, 170, 311, 568, 760
Hpy8I GTNNAC 2 cut(s) 149, 374
HpyAV CCTTC 6 cut(s) 124, 279, 530, 859, 881, 881
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 4 cut(s) 360, 692, 920, 978
HpyF10VI GCNNNNNNNGC 2 cut(s) 303, 512
HpyF3I CTNAG 3 cut(s) 384, 567, 593
HpySE526I ACGT 1 cut(s) 346
Hsp92I GRCGYC 2 cut(s) 201, 330
Hsp92II CATG 5 cut(s) 22, 290, 398, 812, 935
HspAI GCGC 3 cut(s) 23, 58, 176
Kpn2I TCCGGA 1 cut(s) 900
Kzo9I GATC 6 cut(s) 165, 724, 817, 840, 903, 928
LguI GCTCTTC 1 cut(s) 888
LmnI GCTCC 2 cut(s) 503, 903
Lsp1109I GCAGC 3 cut(s) 91, 751, 755
LweI GCATC 2 cut(s) 907, 925
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 954
MalI GATC 6 cut(s) 167, 726, 819, 842, 905, 930
MboI GATC 6 cut(s) 165, 724, 817, 840, 903, 928
MboII GAAGA 4 cut(s) 90, 196, 587, 905
MfeI CAATTG 1 cut(s) 979
MflI RGATCY 1 cut(s) 903
MhlI GDGCHC 2 cut(s) 237, 900
MluCI AATT 2 cut(s) 73, 979
MlyI GAGTC 2 cut(s) 20, 194
Mph1103I ATGCAT 1 cut(s) 694
MroI TCCGGA 1 cut(s) 900
MseI TTAA 3 cut(s) 65, 687, 1006
MslI CAYNNNNRTG 1 cut(s) 813
MspA1I CMGCKG 1 cut(s) 104
MspR9I CCNGG 6 cut(s) 144, 153, 193, 214, 676, 988
MunI CAATTG 1 cut(s) 979
Mva1269I GAATGC 2 cut(s) 604, 781
MvnI CGCG 3 cut(s) 25, 58, 209
MwoI GCNNNNNNNGC 2 cut(s) 303, 512
NciI CCSGG 6 cut(s) 144, 153, 193, 214, 676, 988
NdeII GATC 6 cut(s) 165, 724, 817, 840, 903, 928
NlaIII CATG 5 cut(s) 22, 290, 398, 812, 935
NmeAIII GCCGAG 1 cut(s) 919
NmuCI GTSAC 1 cut(s) 954
NruI TCGCGA 1 cut(s) 209
NsiI ATGCAT 1 cut(s) 694
PagI TCATGA 3 cut(s) 394, 808, 931
PaqCI CACCTGC 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 888
PctI GAATGC 2 cut(s) 604, 781
PfeI GAWTC 3 cut(s) 139, 679, 755
PinAI ACCGGT 1 cut(s) 227
PkrI GCNGC 5 cut(s) 106, 109, 124, 745, 766
PleI GAGTC 2 cut(s) 19, 193
PpsI GAGTC 2 cut(s) 19, 193
PshBI ATTAAT 1 cut(s) 687
PsiI TTATAA 1 cut(s) 804
Psp124BI GAGCTC 2 cut(s) 237, 900
PspFI CCCAGC 1 cut(s) 710
PspPI GGNCC 2 cut(s) 155, 868
PsuI RGATCY 1 cut(s) 903
RruI TCGCGA 1 cut(s) 209
RsaI GTAC 3 cut(s) 584, 659, 669
RsaNI GTAC 3 cut(s) 583, 658, 668
RseI CAYNNNNRTG 1 cut(s) 813
Rsr2I CGGWCCG 1 cut(s) 155
RsrII CGGWCCG 1 cut(s) 155
SacI GAGCTC 2 cut(s) 237, 900
SalI GTCGAC 2 cut(s) 147, 372
SapI GCTCTTC 1 cut(s) 888
SaqAI TTAA 3 cut(s) 65, 687, 1006
SatI GCNGC 5 cut(s) 105, 108, 123, 744, 765
Sau3AI GATC 6 cut(s) 165, 724, 817, 840, 903, 928
Sau96I GGNCC 2 cut(s) 155, 868
SchI GAGTC 2 cut(s) 20, 194
ScrFI CCNGG 6 cut(s) 144, 153, 193, 214, 676, 988
SduI GDGCHC 2 cut(s) 237, 900
SfaNI GCATC 2 cut(s) 907, 925
SfcI CTRYAG 1 cut(s) 750
SinI GGWCC 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 813
Sse9I AATT 2 cut(s) 73, 979
SsiI CCGC 7 cut(s) 102, 108, 120, 123, 158, 246, 478
SstI GAGCTC 2 cut(s) 237, 900
StyD4I CCNGG 6 cut(s) 142, 151, 191, 212, 674, 986
TaiI ACGT 1 cut(s) 349
TaqI TCGA 5 cut(s) 71, 148, 222, 373, 843
TasI AATT 2 cut(s) 73, 979
TauI GCSGC 2 cut(s) 110, 125
TfiI GAWTC 3 cut(s) 139, 679, 755
Tru1I TTAA 3 cut(s) 65, 687, 1006
Tru9I TTAA 3 cut(s) 65, 687, 1006
TscAI CASTG 3 cut(s) 331, 367, 980
TseFI GTSAC 1 cut(s) 954
TseI GCWGC 3 cut(s) 104, 743, 764
Tsp45I GTSAC 1 cut(s) 954
TspDTI ATGAA 3 cut(s) 383, 642, 900
TspGWI ACGGA 2 cut(s) 26, 638
TspRI CASTG 3 cut(s) 331, 367, 980
VpaK11BI GGWCC 1 cut(s) 155
VspI ATTAAT 1 cut(s) 687
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 73
XmiI GTMKAC 2 cut(s) 148, 373
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.