Rw3G019740

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0006586
Physical Location & Seq
Forward (+)
22852499 .. 22857888
5390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G019740.1

Sequence Viewer

Length: 876 bp
ATGCGAATCCGGGTCGACCCGGACCACCTCCGATCCGAGTTGCGCTCACCGAGTCATCCGGGCGAGGCGTCTTCGCGACCCGGAAAATCGAGACCGGTGAGCTCTTACACACCGCCAAACCCATTCTGTCTCACCCTTCTCTCTCCGCCCTCCATAAGGTCTGCTACTTCTGCCTCCGAAAGCTCAAAACCACCGACGCCCTCTCAACCCCAAGGCGTTTCCTATTGCAGTGAGGAGTGTCGACAAGAAGCACAGGGATTTCATGATGTTGAGATGACGGCAGATTGGTCAGCTTATGATGACTATTGCCGGTCTAATGGTTTGAAATATCCTCTACTTGTAAAGCGGTTGGCTTGTATGGTTATGTCAGGAGCTACACCTGCCAACCTTCTTGACATACTCCAACCTGCCAGCTTATCTCCAGAGATGATTTCTGAGATGGAAGAGGGGTACGGCTTGCTAAGGAATGCCTACATAAACTCAAATATCACGGATGAACAGATGTCCTTTTTGACTAAACAATGGTACATCGGCATACTTTCCCGGATTCGTATTAATGCATTTCGTATTGAGTTGGCTGGGGGATACGATGATCTCCTGTCATCATTAGCAGCCTCTATAGAATCTGAAGCTGCTGTTGGGAATGGTGTTTATATGCTTCCATCCTTTTATAATCATGATTGTGATCCAAATGCCCACATTATATGGATAGAGAATGCAGATGCGAGATTGAAGGCCCTTCGTGATGTGGATGAAGGGGAAGAGCTCCGGATCTGCTATATTGATGCAAGTATGGATCATGATGCTCGGCAGAGTTTCCTGTCACAAGGGTTTGGTTTTCAGTGCAATTGTCCCCGGTGTTTGTCTGGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

291

Amino Acids

32.23

Weight (kDa)

4.91

Isoelectric Point (pI)

72.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 206 - 260 1.5e-08 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 672
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 388, 415
AccI GTMKAC 2 cut(s) 15, 241
AccII CGCG 1 cut(s) 76
AccIII TCCGGA 1 cut(s) 768
AciI CCGC 3 cut(s) 113, 146, 346
AclWI GGATC 4 cut(s) 27, 680, 779, 804
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 2 cut(s) 68, 197
AfaI GTAC 2 cut(s) 452, 527
AfiI CCNNNNNNNGG 1 cut(s) 156
AgeI ACCGGT 1 cut(s) 94
AgsI TTSAA 2 cut(s) 325, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 621, 653
AluBI AGCT 7 cut(s) 102, 183, 293, 374, 414, 632, 766
AluI AGCT 7 cut(s) 102, 183, 293, 374, 414, 632, 766
Alw21I GWGCWC 2 cut(s) 104, 768
Alw26I GTCTC 2 cut(s) 85, 134
AlwI GGATC 4 cut(s) 27, 680, 779, 804
Aor13HI TCCGGA 1 cut(s) 768
AoxI GGCC 1 cut(s) 735
ApeKI GCWGC 2 cut(s) 611, 632
AseI ATTAAT 1 cut(s) 555
AsiGI ACCGGT 1 cut(s) 94
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 22, 736
AsuC2I CCSGG 6 cut(s) 11, 20, 60, 81, 544, 856
AsuHPI GGTGA 3 cut(s) 39, 109, 124
AvaII GGWCC 1 cut(s) 22
BanII GRGCYC 2 cut(s) 104, 768
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 104, 768
BbvI GCAGC 2 cut(s) 619, 623
BccI CCATC 2 cut(s) 433, 670
BceAI ACGGC 2 cut(s) 294, 469
BciVI GTATCC 1 cut(s) 578
BcnI CCSGG 6 cut(s) 11, 20, 60, 81, 544, 856
BcoDI GTCTC 2 cut(s) 85, 134
BfmI CTRYAG 1 cut(s) 618
BfuAI ACCTGC 2 cut(s) 388, 415
BfuI GTATCC 1 cut(s) 578
BisI GCNGC 2 cut(s) 612, 633
BlsI GCNGC 2 cut(s) 613, 634
Bme1390I CCNGG 6 cut(s) 11, 20, 60, 81, 544, 856
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 22, 736
BmrFI CCNGG 6 cut(s) 11, 20, 60, 81, 544, 856
BmsI GCATC 3 cut(s) 712, 775, 793
BpiI GAAGAC 1 cut(s) 63
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 1 cut(s) 405
Bpu10I CCTNAGC 1 cut(s) 461
BpuMI CCSGG 6 cut(s) 11, 20, 60, 81, 544, 856
BsaBI GATNNNNATC 1 cut(s) 684
BsaHI GRCGYC 2 cut(s) 68, 197
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 211, 854
BsaWI WCCGGW 2 cut(s) 94, 768
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse118I RCCGGY 2 cut(s) 94, 309
Bse8I GATNNNNATC 1 cut(s) 684
BseAI TCCGGA 1 cut(s) 768
BseDI CCNNGG 2 cut(s) 211, 854
BseGI GGATG 4 cut(s) 55, 499, 662, 757
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 426
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 2 cut(s) 619, 623
BseYI CCCAGC 1 cut(s) 578
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 1 cut(s) 737
BshTI ACCGGT 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 104, 768
BsiSI CCGG 9 cut(s) 10, 20, 59, 81, 95, 310, 544, 769, 856
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 85, 134
BsmFI GGGAC 1 cut(s) 837
BsmI GAATGC 2 cut(s) 472, 721
BsnI GGCC 1 cut(s) 737
Bso31I GGTCTC 1 cut(s) 85
Bsp1286I GDGCHC 2 cut(s) 104, 768
Bsp13I TCCGGA 1 cut(s) 768
Bsp143I GATC 5 cut(s) 32, 592, 685, 771, 796
Bsp68I TCGCGA 1 cut(s) 76
BspACI CCGC 3 cut(s) 113, 146, 346
BspANI GGCC 1 cut(s) 737
BspCNI CTCAG 1 cut(s) 427
BspEI TCCGGA 1 cut(s) 768
BspFNI CGCG 1 cut(s) 76
BspHI TCATGA 3 cut(s) 262, 676, 799
BspMI ACCTGC 2 cut(s) 388, 415
BspPI GGATC 4 cut(s) 27, 680, 779, 804
BspQI GCTCTTC 1 cut(s) 756
BspTNI GGTCTC 1 cut(s) 85
BsrFI RCCGGY 2 cut(s) 94, 309
BssAI RCCGGY 2 cut(s) 94, 309
BssECI CCNNGG 2 cut(s) 211, 854
BssMI GATC 5 cut(s) 32, 592, 685, 771, 796
BssNI GRCGYC 2 cut(s) 68, 197
BssT1I CCWWGG 1 cut(s) 211
Bst6I CTCTTC 2 cut(s) 438, 756
BstACI GRCGYC 2 cut(s) 68, 197
BstC8I GCNNGC 2 cut(s) 412, 458
BstDEI CTNAG 2 cut(s) 435, 461
BstENI CCTNNNNNAGG 1 cut(s) 154
BstF5I GGATG 4 cut(s) 55, 499, 662, 757
BstFNI CGCG 1 cut(s) 76
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 5 cut(s) 35, 595, 688, 774, 799
BstMAI GTCTC 2 cut(s) 85, 134
BstMBI GATC 5 cut(s) 32, 592, 685, 771, 796
BstMWI GCNNNNNNNGC 2 cut(s) 170, 380
BstSCI CCNGG 6 cut(s) 9, 18, 58, 79, 542, 854
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 2 cut(s) 619, 623
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 771
BstYI RGATCY 1 cut(s) 771
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 1 cut(s) 737
BtsCI GGATG 4 cut(s) 55, 499, 662, 757
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 2 cut(s) 235, 848
BtuMI TCGCGA 1 cut(s) 76
BveI ACCTGC 2 cut(s) 388, 415
Cac8I GCNNGC 2 cut(s) 412, 458
CciI TCATGA 3 cut(s) 262, 676, 799
CfoI GCGC 1 cut(s) 45
Cfr10I RCCGGY 2 cut(s) 94, 309
Cfr13I GGNCC 2 cut(s) 22, 736
CseI GACGC 2 cut(s) 57, 205
Csp6I GTAC 2 cut(s) 451, 526
CspAI ACCGGT 1 cut(s) 94
CviAII CATG 3 cut(s) 263, 677, 800
CviQI GTAC 2 cut(s) 451, 526
DdeI CTNAG 2 cut(s) 435, 461
DpnI GATC 5 cut(s) 34, 594, 687, 773, 798
DpnII GATC 5 cut(s) 32, 592, 685, 771, 796
Eam1104I CTCTTC 2 cut(s) 438, 756
EarI CTCTTC 2 cut(s) 438, 756
EciI GGCGGA 1 cut(s) 135
Ecl136II GAGCTC 2 cut(s) 102, 766
Eco130I CCWWGG 1 cut(s) 211
Eco24I GRGCYC 2 cut(s) 104, 768
Eco31I GGTCTC 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 22
Eco53kI GAGCTC 2 cut(s) 102, 766
Eco57I CTGAAG 1 cut(s) 648
EcoICRI GAGCTC 2 cut(s) 102, 766
EcoNI CCTNNNNNAGG 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 562
EcoT38I GRGCYC 2 cut(s) 104, 768
ErhI CCWWGG 1 cut(s) 211
FaeI CATG 3 cut(s) 266, 680, 803
FaqI GGGAC 1 cut(s) 837
FatI CATG 3 cut(s) 262, 676, 799
FblI GTMKAC 2 cut(s) 15, 241
Fnu4HI GCNGC 2 cut(s) 612, 633
FokI GGATG 4 cut(s) 42, 506, 649, 764
FriOI GRGCYC 2 cut(s) 104, 768
Fsp4HI GCNGC 2 cut(s) 612, 633
GlaI GCGC 1 cut(s) 44
GluI GCNGC 2 cut(s) 612, 633
GsaI CCCAGC 1 cut(s) 582
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 1 cut(s) 737
HapII CCGG 9 cut(s) 10, 20, 59, 81, 95, 310, 544, 769, 856
HgaI GACGC 2 cut(s) 57, 205
HhaI GCGC 1 cut(s) 45
Hin1I GRCGYC 2 cut(s) 68, 197
Hin1II CATG 3 cut(s) 266, 680, 803
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HincII GTYRAC 2 cut(s) 16, 242
HindII GTYRAC 2 cut(s) 16, 242
HinfI GANTC 4 cut(s) 6, 52, 547, 623
HpaII CCGG 9 cut(s) 10, 20, 59, 81, 95, 310, 544, 769, 856
HphI GGTGA 3 cut(s) 39, 109, 124
Hpy166II GTNNAC 2 cut(s) 16, 242
Hpy188I TCNGA 5 cut(s) 32, 37, 178, 436, 628
Hpy8I GTNNAC 2 cut(s) 16, 242
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 5 cut(s) 146, 398, 727, 749, 749
HpyCH4V TGCA 5 cut(s) 228, 560, 719, 788, 846
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 380
HpyF3I CTNAG 2 cut(s) 435, 461
Hsp92I GRCGYC 2 cut(s) 68, 197
Hsp92II CATG 3 cut(s) 266, 680, 803
HspAI GCGC 1 cut(s) 43
Kpn2I TCCGGA 1 cut(s) 768
Kzo9I GATC 5 cut(s) 32, 592, 685, 771, 796
LguI GCTCTTC 1 cut(s) 756
LmnI GCTCC 2 cut(s) 371, 771
Lsp1109I GCAGC 2 cut(s) 619, 623
LweI GCATC 3 cut(s) 712, 775, 793
MaeIII GTNAC 1 cut(s) 822
MalI GATC 5 cut(s) 34, 594, 687, 773, 798
MboI GATC 5 cut(s) 32, 592, 685, 771, 796
MboII GAAGA 3 cut(s) 63, 455, 773
MfeI CAATTG 1 cut(s) 847
MflI RGATCY 1 cut(s) 771
MhlI GDGCHC 2 cut(s) 104, 768
MluCI AATT 1 cut(s) 847
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 9 cut(s) 38, 58, 160, 184, 211, 226, 342, 439, 625
Mph1103I ATGCAT 1 cut(s) 562
MroI TCCGGA 1 cut(s) 768
MseI TTAA 2 cut(s) 555, 874
MslI CAYNNNNRTG 1 cut(s) 681
MspI CCGG 9 cut(s) 10, 20, 59, 81, 95, 310, 544, 769, 856
MspR9I CCNGG 6 cut(s) 11, 20, 60, 81, 544, 856
MunI CAATTG 1 cut(s) 847
Mva1269I GAATGC 2 cut(s) 472, 721
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 170, 380
NciI CCSGG 6 cut(s) 11, 20, 60, 81, 544, 856
NdeII GATC 5 cut(s) 32, 592, 685, 771, 796
NlaIII CATG 3 cut(s) 266, 680, 803
NmeAIII GCCGAG 1 cut(s) 787
NmuCI GTSAC 1 cut(s) 822
NruI TCGCGA 1 cut(s) 76
NsiI ATGCAT 1 cut(s) 562
PagI TCATGA 3 cut(s) 262, 676, 799
PaqCI CACCTGC 1 cut(s) 388
PciSI GCTCTTC 1 cut(s) 756
PctI GAATGC 2 cut(s) 472, 721
PfeI GAWTC 3 cut(s) 6, 547, 623
PfoI TCCNGGA 1 cut(s) 542
PinAI ACCGGT 1 cut(s) 94
PkrI GCNGC 2 cut(s) 613, 634
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
PshBI ATTAAT 1 cut(s) 555
PsiI TTATAA 1 cut(s) 672
Psp124BI GAGCTC 2 cut(s) 104, 768
PspFI CCCAGC 1 cut(s) 578
PspPI GGNCC 2 cut(s) 22, 736
PsuI RGATCY 1 cut(s) 771
RruI TCGCGA 1 cut(s) 76
RsaI GTAC 2 cut(s) 452, 527
RsaNI GTAC 2 cut(s) 451, 526
RseI CAYNNNNRTG 1 cut(s) 681
SacI GAGCTC 2 cut(s) 104, 768
SalI GTCGAC 2 cut(s) 14, 240
SapI GCTCTTC 1 cut(s) 756
SaqAI TTAA 2 cut(s) 555, 874
SatI GCNGC 2 cut(s) 612, 633
Sau3AI GATC 5 cut(s) 32, 592, 685, 771, 796
Sau96I GGNCC 2 cut(s) 22, 736
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 6 cut(s) 11, 20, 60, 81, 544, 856
SduI GDGCHC 2 cut(s) 104, 768
SfaNI GCATC 3 cut(s) 712, 775, 793
SfcI CTRYAG 1 cut(s) 618
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 681
Sse9I AATT 1 cut(s) 847
SsiI CCGC 3 cut(s) 113, 146, 346
SstI GAGCTC 2 cut(s) 104, 768
StyD4I CCNGG 6 cut(s) 9, 18, 58, 79, 542, 854
StyI CCWWGG 1 cut(s) 211
TaqI TCGA 3 cut(s) 15, 89, 241
TasI AATT 1 cut(s) 847
TfiI GAWTC 3 cut(s) 6, 547, 623
Tru1I TTAA 2 cut(s) 555, 874
Tru9I TTAA 2 cut(s) 555, 874
TscAI CASTG 2 cut(s) 235, 848
TseFI GTSAC 1 cut(s) 822
TseI GCWGC 2 cut(s) 611, 632
Tsp45I GTSAC 1 cut(s) 822
TspDTI ATGAA 3 cut(s) 251, 510, 768
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 2 cut(s) 235, 848
VpaK11BI GGWCC 1 cut(s) 22
VspI ATTAAT 1 cut(s) 555
XagI CCTNNNNNAGG 1 cut(s) 154
XmiI GTMKAC 2 cut(s) 15, 241
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.