Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0006586

Physical Location & Seq

Genomic Coordinates Forward (+)

4460324 .. 4464015

Gene Structure 3692 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g04590.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGGGACTGGTCCGTTATAGCCGTTGGGCTTCGCGCCTCAAAACATTGAACTCCCAATGCAAGCCTTTCCTCTCCGCCACCTCCTCCTTCTCCTCCGGCACCACAGCCGGGAATGAGAATCCAGGTCGACCCGGACCGCCTCCGATTCAAGTCGCGCTCACAGAGTCATCCGGGCGCGGCGTCTTCGCTACCCGGAAAATCGAGAACGGCGAGCTCATACACACAGCCAAGCCCGTCCTGTCTCACCCTTCCCTCTCCACCGTCCACCGGGTCTGTTATTTTTGCCTCAGAAAGCTCAGAAACACGGAGACCTCCCAACCCCAACGTGTTTCGTTTTGCAGCGACGATTGCAAAGAGCAAGCTAAGGGTTTTTATGATGTTGAGATGAGAGCAGATTGGTCTGTTTATGATGACTATTGCAGGTCCCATGGTTTGAAATACCCACTTCTTGTAAAGCGGTTGGCTTGTATGGTCATGTTGGGAGCTGCACCTGCCAATCTTCTTGACATACTCCAACCTGCTAGGTTATCCGCGGAGATGATTTCAGAGATGGAAGCGGGGTTTGGCTTGCTAAGGAGTACCTTCAGAAACTCAAATATCACGGATGAACAGATGTCATTTTTAACTAAGCAATGGTATATTGGCATACTGGCACGCATTCGCATAAATGCATTTCGAATTGAGTTGGTTGGGGGACTGTATGATGATCTCCTGTCATCAGCAGCAGCATCTATAGATGCTGAAGCTGCTGTCGGGAATGCTGTTTATATCCTTCCATCGTTCTATAATCATGATTGTGATCCAAATGCCCACATTATATGGATAGAGAACACAGATGCAAGATTGAAGGCCCTACGCAATGTGGATGCAGGTGAAGAGCTCCGGATCTGCTACATCGATGCGAGCATGGATCATGATGCCCGGCAGAGTATACTGTCCCAGGGGTTTGGTTTTCAGTGCAGTTGCCTTCGGTGTCTGTCCGGTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SET

Protein Analysis

330

Amino Acids

36.51

Weight (kDa)

7.02

Isoelectric Point (pI)

53.92

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06620 AT5G06620
fragaria_vesca	FvH4_6g21530 FvH4_6g21530 FvH4_6g21530 FvH4_6g21530 FvH4_6g21530
malus_domestica	MD12G1052100.v1.1
prunus_persica	Prupe.7G085000_v2.0.a1 Prupe.7G085000_v2.0.a1 Prupe.7G085000_v2.0.a1 Prupe.7G085000_v2.0.a1
pyrus_communis	pycom12g04590
rosa_chinensis	RchiOBHm_Chr3g0478741 RchiOBHm_Chr3g0478761
rosa_laevigata	RLG00000023628
rosa_multiflora	Rmu_sc0001519.1_g000008
rosa_roxburghii	Rroxscaffold_6G00403110
rosa_rugosa	Rorug03G0167600 Rorug03G0167700 Rorug03G0167800
rosa_samantha	Rh3AG219700 Rh3BG251500 Rh3CG248500 Rh3DG244700
rosa_wichuraiana	Rw3G019740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	499, 860
Acc36I	ACCTGC	4 cut(s)	411, 499, 526, 860
AccB1I	GGYRCC	1 cut(s)	98
AccI	GTMKAC	2 cut(s)	127, 931
AccII	CGCG	4 cut(s)	34, 155, 177, 533
AccIII	TCCGGA	1 cut(s)	882
AciI	CCGC	7 cut(s)	75, 137, 177, 457, 531, 533, 557
AclWI	GGATC	3 cut(s)	794, 893, 918
AcuI	CTGAAG	2 cut(s)	568, 762
AcyI	GRCGYC	1 cut(s)	180
AfaI	GTAC	1 cut(s)	580
AfiI	CCNNNNNNNGG	2 cut(s)	108, 267
AflIII	ACRYGT	1 cut(s)	325
AgsI	TTSAA	4 cut(s)	49, 149, 436, 847
AjnI	CCWGG	2 cut(s)	121, 939
AjuI	GAANNNNNNNTTGG	2 cut(s)	546, 578
AluBI	AGCT	6 cut(s)	214, 295, 362, 485, 746, 880
AluI	AGCT	6 cut(s)	214, 295, 362, 485, 746, 880
Alw21I	GWGCWC	2 cut(s)	216, 882
Alw26I	GTCTC	2 cut(s)	246, 302
AlwI	GGATC	3 cut(s)	794, 893, 918
Aor13HI	TCCGGA	1 cut(s)	882
AoxI	GGCC	1 cut(s)	849
ApeKI	GCWGC	5 cut(s)	339, 485, 722, 725, 746
AspLEI	GCGC	3 cut(s)	36, 157, 177
AspS9I	GGNCC	4 cut(s)	10, 134, 423, 850
AsuC2I	CCSGG	6 cut(s)	109, 132, 172, 193, 269, 922
AsuHPI	GGTGA	2 cut(s)	236, 884
AsuII	TTCGAA	1 cut(s)	676
AvaII	GGWCC	3 cut(s)	10, 134, 423
BanI	GGYRCC	1 cut(s)	98
BanII	GRGCYC	2 cut(s)	216, 882
BbsI	GAAGAC	1 cut(s)	175
Bbv12I	GWGCWC	2 cut(s)	216, 882
BbvI	GCAGC	5 cut(s)	351, 472, 733, 734, 737
BccI	CCATC	2 cut(s)	544, 784
BceAI	ACGGC	2 cut(s)	6, 223
BciT130I	CCWGG	2 cut(s)	123, 941
BcnI	CCSGG	6 cut(s)	109, 132, 172, 193, 269, 922
BcoDI	GTCTC	2 cut(s)	246, 302
BfaI	CTAG	1 cut(s)	522
BfmI	CTRYAG	1 cut(s)	732
BfuAI	ACCTGC	4 cut(s)	411, 499, 526, 860
BisI	GCNGC	6 cut(s)	178, 340, 486, 723, 726, 747
BlsI	GCNGC	6 cut(s)	179, 341, 487, 724, 727, 748
Bme1390I	CCNGG	8 cut(s)	109, 123, 132, 172, 193, 269, 922, 941
Bme18I	GGWCC	3 cut(s)	10, 134, 423
BmgT120I	GGNCC	4 cut(s)	10, 134, 423, 850
BmiI	GGNNCC	2 cut(s)	100, 425
BmrFI	CCNGG	8 cut(s)	109, 123, 132, 172, 193, 269, 922, 941
BmsI	GCATC	6 cut(s)	727, 737, 826, 856, 889, 907
BpiI	GAAGAC	1 cut(s)	175
Bpu10I	CCTNAGC	2 cut(s)	363, 572
Bpu14I	TTCGAA	1 cut(s)	676
BpuMI	CCSGG	6 cut(s)	109, 132, 172, 193, 269, 922
Bsa29I	ATCGAT	1 cut(s)	897
BsaBI	GATNNNNATC	1 cut(s)	798
BsaHI	GRCGYC	1 cut(s)	180
BsaI	GGTCTC	1 cut(s)	302
BsaJI	CCNNGG	4 cut(s)	427, 531, 939, 940
BsaWI	WCCGGW	2 cut(s)	882, 980
Bsc4I	CCNNNNNNNGG	2 cut(s)	108, 267
Bse1I	ACTGG	2 cut(s)	12, 654
Bse3DI	GCAATG	2 cut(s)	638, 865
Bse8I	GATNNNNATC	1 cut(s)	798
BseAI	TCCGGA	1 cut(s)	882
BseBI	CCWGG	2 cut(s)	123, 941
BseCI	ATCGAT	1 cut(s)	897
BseDI	CCNNGG	4 cut(s)	427, 531, 939, 940
BseGI	GGATG	3 cut(s)	167, 610, 871
BseJI	GATNNNNATC	1 cut(s)	798
BseLI	CCNNNNNNNGG	2 cut(s)	108, 267
BseMI	GCAATG	2 cut(s)	638, 865
BseMII	CTCAG	2 cut(s)	301, 310
BseNI	ACTGG	2 cut(s)	12, 654
BseRI	GAGGAG	2 cut(s)	73, 82
BseXI	GCAGC	5 cut(s)	351, 472, 733, 734, 737
BsgI	GTGCAG	2 cut(s)	471, 979
Bsh1236I	CGCG	4 cut(s)	34, 155, 177, 533
BshFI	GGCC	1 cut(s)	851
BshNI	GGYRCC	1 cut(s)	98
BshVI	ATCGAT	1 cut(s)	897
BsiHKAI	GWGCWC	2 cut(s)	216, 882
BsiSI	CCGG	9 cut(s)	96, 108, 132, 171, 193, 268, 883, 922, 981
BslFI	GGGAC	4 cut(s)	18, 409, 708, 922
BslI	CCNNNNNNNGG	2 cut(s)	108, 267
BsmAI	GTCTC	2 cut(s)	246, 302
BsmFI	GGGAC	4 cut(s)	18, 409, 708, 922
BsmI	GAATGC	2 cut(s)	657, 763
BsnI	GGCC	1 cut(s)	851
Bso31I	GGTCTC	1 cut(s)	302
Bsp119I	TTCGAA	1 cut(s)	676
Bsp1286I	GDGCHC	2 cut(s)	216, 882
Bsp13I	TCCGGA	1 cut(s)	882
Bsp143I	GATC	4 cut(s)	706, 799, 885, 910
Bsp19I	CCATGG	1 cut(s)	427
BspACI	CCGC	7 cut(s)	75, 137, 177, 457, 531, 533, 557
BspANI	GGCC	1 cut(s)	851
BspCNI	CTCAG	2 cut(s)	300, 309
BspDI	ATCGAT	1 cut(s)	897
BspEI	TCCGGA	1 cut(s)	882
BspFNI	CGCG	4 cut(s)	34, 155, 177, 533
BspHI	TCATGA	2 cut(s)	790, 913
BspLI	GGNNCC	2 cut(s)	100, 425
BspMI	ACCTGC	4 cut(s)	411, 499, 526, 860
BspPI	GGATC	3 cut(s)	794, 893, 918
BspQI	GCTCTTC	1 cut(s)	870
BspT104I	TTCGAA	1 cut(s)	676
BspT107I	GGYRCC	1 cut(s)	98
BspTNI	GGTCTC	1 cut(s)	302
BsrDI	GCAATG	2 cut(s)	638, 865
BsrI	ACTGG	2 cut(s)	12, 654
BssECI	CCNNGG	4 cut(s)	427, 531, 939, 940
BssMI	GATC	4 cut(s)	706, 799, 885, 910
BssNAI	GTATAC	1 cut(s)	932
BssNI	GRCGYC	1 cut(s)	180
BssT1I	CCWWGG	1 cut(s)	427
Bst1107I	GTATAC	1 cut(s)	932
Bst2UI	CCWGG	2 cut(s)	123, 941
Bst4CI	ACNGT	3 cut(s)	262, 699, 936
Bst6I	CTCTTC	1 cut(s)	870
BstACI	GRCGYC	1 cut(s)	180
BstBI	TTCGAA	1 cut(s)	676
BstC8I	GCNNGC	6 cut(s)	62, 212, 360, 569, 655, 904
BstDEI	CTNAG	5 cut(s)	287, 296, 363, 572, 627
BstDSI	CCRYGG	2 cut(s)	427, 531
BstF5I	GGATG	3 cut(s)	167, 610, 871
BstFNI	CGCG	4 cut(s)	34, 155, 177, 533
BstHHI	GCGC	3 cut(s)	36, 157, 177
BstKTI	GATC	4 cut(s)	709, 802, 888, 913
BstMAI	GTCTC	2 cut(s)	246, 302
BstMBI	GATC	4 cut(s)	706, 799, 885, 910
BstMWI	GCNNNNNNNGC	3 cut(s)	348, 491, 746
BstNI	CCWGG	2 cut(s)	123, 941
BstSCI	CCNGG	8 cut(s)	107, 121, 130, 170, 191, 267, 920, 939
BstSFI	CTRYAG	1 cut(s)	732
BstUI	CGCG	4 cut(s)	34, 155, 177, 533
BstV1I	GCAGC	5 cut(s)	351, 472, 733, 734, 737
BstV2I	GAAGAC	1 cut(s)	175
BstX2I	RGATCY	1 cut(s)	885
BstXI	CCANNNNNNTGG	1 cut(s)	947
BstYI	RGATCY	1 cut(s)	885
BstZ17I	GTATAC	1 cut(s)	932
Bsu15I	ATCGAT	1 cut(s)	897
BsuRI	GGCC	1 cut(s)	851
BsuTUI	ATCGAT	1 cut(s)	897
BtgI	CCRYGG	2 cut(s)	427, 531
BtsCI	GGATG	3 cut(s)	167, 610, 871
BtsIMutI	CAGTG	1 cut(s)	962
BveI	ACCTGC	4 cut(s)	411, 499, 526, 860
Cac8I	GCNNGC	6 cut(s)	62, 212, 360, 569, 655, 904
CciI	TCATGA	2 cut(s)	790, 913
CfoI	GCGC	3 cut(s)	36, 157, 177
Cfr13I	GGNCC	4 cut(s)	10, 134, 423, 850
Cfr42I	CCGCGG	1 cut(s)	534
ClaI	ATCGAT	1 cut(s)	897
CpoI	CGGWCCG	1 cut(s)	134
CseI	GACGC	1 cut(s)	169
Csp6I	GTAC	1 cut(s)	579
CspI	CGGWCCG	1 cut(s)	134
CviAII	CATG	5 cut(s)	428, 475, 791, 907, 914
CviQI	GTAC	1 cut(s)	579
DdeI	CTNAG	5 cut(s)	287, 296, 363, 572, 627
DpnI	GATC	4 cut(s)	708, 801, 887, 912
DpnII	GATC	4 cut(s)	706, 799, 885, 910
Eam1104I	CTCTTC	1 cut(s)	870
EarI	CTCTTC	1 cut(s)	870
EciI	GGCGGA	1 cut(s)	64
Ecl136II	GAGCTC	2 cut(s)	214, 880
Eco130I	CCWWGG	1 cut(s)	427
Eco24I	GRGCYC	2 cut(s)	216, 882
Eco31I	GGTCTC	1 cut(s)	302
Eco47I	GGWCC	3 cut(s)	10, 134, 423
Eco53kI	GAGCTC	2 cut(s)	214, 880
Eco57I	CTGAAG	2 cut(s)	568, 762
EcoICRI	GAGCTC	2 cut(s)	214, 880
EcoO109I	RGGNCCY	2 cut(s)	423, 850
EcoRII	CCWGG	2 cut(s)	121, 939
EcoT14I	CCWWGG	1 cut(s)	427
EcoT22I	ATGCAT	1 cut(s)	673
EcoT38I	GRGCYC	2 cut(s)	216, 882
ErhI	CCWWGG	1 cut(s)	427
FaeI	CATG	5 cut(s)	431, 478, 794, 910, 917
FaqI	GGGAC	4 cut(s)	18, 409, 708, 922
FatI	CATG	5 cut(s)	427, 474, 790, 906, 913
FauI	CCCGC	1 cut(s)	550
FblI	GTMKAC	2 cut(s)	127, 931
Fnu4HI	GCNGC	6 cut(s)	178, 340, 486, 723, 726, 747
FokI	GGATG	3 cut(s)	154, 617, 878
FriOI	GRGCYC	2 cut(s)	216, 882
Fsp4HI	GCNGC	6 cut(s)	178, 340, 486, 723, 726, 747
FspBI	CTAG	1 cut(s)	522
GlaI	GCGC	3 cut(s)	35, 156, 176
GluI	GCNGC	6 cut(s)	178, 340, 486, 723, 726, 747
HaeIII	GGCC	1 cut(s)	851
HapII	CCGG	9 cut(s)	96, 108, 132, 171, 193, 268, 883, 922, 981
HgaI	GACGC	1 cut(s)	169
HhaI	GCGC	3 cut(s)	36, 157, 177
Hin1I	GRCGYC	1 cut(s)	180
Hin1II	CATG	5 cut(s)	431, 478, 794, 910, 917
Hin6I	GCGC	3 cut(s)	34, 155, 175
HinP1I	GCGC	3 cut(s)	34, 155, 175
HincII	GTYRAC	1 cut(s)	128
HindII	GTYRAC	1 cut(s)	128
HinfI	GANTC	3 cut(s)	118, 145, 164
HpaII	CCGG	9 cut(s)	96, 108, 132, 171, 193, 268, 883, 922, 981
HphI	GGTGA	2 cut(s)	236, 884
Hpy166II	GTNNAC	3 cut(s)	128, 265, 932
Hpy188I	TCNGA	5 cut(s)	144, 290, 299, 547, 587
Hpy188III	TCNNGA	6 cut(s)	202, 503, 754, 791, 883, 914
Hpy8I	GTNNAC	3 cut(s)	128, 265, 932
Hpy99I	CGWCG	1 cut(s)	347
HpyAV	CCTTC	6 cut(s)	97, 258, 592, 782, 841, 977
HpyCH4III	ACNGT	3 cut(s)	262, 699, 936
HpyCH4IV	ACGT	1 cut(s)	325
HpyCH4V	TGCA	9 cut(s)	60, 339, 351, 420, 488, 671, 839, 869, 960
HpyF10VI	GCNNNNNNNGC	3 cut(s)	348, 491, 746
HpyF3I	CTNAG	5 cut(s)	287, 296, 363, 572, 627
HpySE526I	ACGT	1 cut(s)	325
Hsp92I	GRCGYC	1 cut(s)	180
Hsp92II	CATG	5 cut(s)	431, 478, 794, 910, 917
HspAI	GCGC	3 cut(s)	34, 155, 175
Kpn2I	TCCGGA	1 cut(s)	882
KspI	CCGCGG	1 cut(s)	534
Kzo9I	GATC	4 cut(s)	706, 799, 885, 910
LguI	GCTCTTC	1 cut(s)	870
LmnI	GCTCC	2 cut(s)	482, 885
Lsp1109I	GCAGC	5 cut(s)	351, 472, 733, 734, 737
LweI	GCATC	6 cut(s)	727, 737, 826, 856, 889, 907
MaeI	CTAG	1 cut(s)	522
MaeII	ACGT	1 cut(s)	325
MalI	GATC	4 cut(s)	708, 801, 887, 912
MboI	GATC	4 cut(s)	706, 799, 885, 910
MboII	GAAGA	3 cut(s)	175, 491, 887
MflI	RGATCY	1 cut(s)	885
MhlI	GDGCHC	2 cut(s)	216, 882
MluCI	AATT	1 cut(s)	678
MlyI	GAGTC	1 cut(s)	173
MmeI	TCCRAC	1 cut(s)	538
MnlI	CCTC	9 cut(s)	47, 80, 91, 94, 103, 150, 263, 296, 322
Mph1103I	ATGCAT	1 cut(s)	673
MroI	TCCGGA	1 cut(s)	882
MseI	TTAA	2 cut(s)	623, 988
MslI	CAYNNNNRTG	1 cut(s)	795
MspA1I	CMGCKG	1 cut(s)	533
MspI	CCGG	9 cut(s)	96, 108, 132, 171, 193, 268, 883, 922, 981
MspR9I	CCNGG	8 cut(s)	109, 123, 132, 172, 193, 269, 922, 941
Mva1269I	GAATGC	2 cut(s)	657, 763
MvaI	CCWGG	2 cut(s)	123, 941
MvnI	CGCG	4 cut(s)	34, 155, 177, 533
MwoI	GCNNNNNNNGC	3 cut(s)	348, 491, 746
NciI	CCSGG	6 cut(s)	109, 132, 172, 193, 269, 922
NcoI	CCATGG	1 cut(s)	427
NdeII	GATC	4 cut(s)	706, 799, 885, 910
NlaIII	CATG	5 cut(s)	431, 478, 794, 910, 917
NlaIV	GGNNCC	2 cut(s)	100, 425
NsiI	ATGCAT	1 cut(s)	673
NspV	TTCGAA	1 cut(s)	676
PagI	TCATGA	2 cut(s)	790, 913
PaqCI	CACCTGC	2 cut(s)	499, 860
PasI	CCCWGGG	1 cut(s)	940
PciSI	GCTCTTC	1 cut(s)	870
PcsI	WCGNNNNNNNCGW	1 cut(s)	207
PctI	GAATGC	2 cut(s)	657, 763
PfeI	GAWTC	2 cut(s)	118, 145
PkrI	GCNGC	6 cut(s)	179, 341, 487, 724, 727, 748
PleI	GAGTC	1 cut(s)	172
PpsI	GAGTC	1 cut(s)	172
PpuMI	RGGWCCY	1 cut(s)	423
Psp124BI	GAGCTC	2 cut(s)	216, 882
Psp5II	RGGWCCY	1 cut(s)	423
Psp6I	CCWGG	2 cut(s)	121, 939
PspGI	CCWGG	2 cut(s)	121, 939
PspN4I	GGNNCC	2 cut(s)	100, 425
PspPI	GGNCC	4 cut(s)	10, 134, 423, 850
PspPPI	RGGWCCY	1 cut(s)	423
PsuI	RGATCY	1 cut(s)	885
RsaI	GTAC	1 cut(s)	580
RsaNI	GTAC	1 cut(s)	579
RseI	CAYNNNNRTG	1 cut(s)	795
Rsr2I	CGGWCCG	1 cut(s)	134
RsrII	CGGWCCG	1 cut(s)	134
SacI	GAGCTC	2 cut(s)	216, 882
SacII	CCGCGG	1 cut(s)	534
SalI	GTCGAC	1 cut(s)	126
SapI	GCTCTTC	1 cut(s)	870
SaqAI	TTAA	2 cut(s)	623, 988
SatI	GCNGC	6 cut(s)	178, 340, 486, 723, 726, 747
Sau3AI	GATC	4 cut(s)	706, 799, 885, 910
Sau96I	GGNCC	4 cut(s)	10, 134, 423, 850
SchI	GAGTC	1 cut(s)	173
ScrFI	CCNGG	8 cut(s)	109, 123, 132, 172, 193, 269, 922, 941
SduI	GDGCHC	2 cut(s)	216, 882
SfaNI	GCATC	6 cut(s)	727, 737, 826, 856, 889, 907
SfcI	CTRYAG	1 cut(s)	732
Sfr303I	CCGCGG	1 cut(s)	534
SfuI	TTCGAA	1 cut(s)	676
SgrBI	CCGCGG	1 cut(s)	534
SinI	GGWCC	3 cut(s)	10, 134, 423
SmiMI	CAYNNNNRTG	1 cut(s)	795
Sse9I	AATT	1 cut(s)	678
SsiI	CCGC	7 cut(s)	75, 137, 177, 457, 531, 533, 557
SspMI	CTAG	1 cut(s)	522
SstI	GAGCTC	2 cut(s)	216, 882
StyD4I	CCNGG	8 cut(s)	107, 121, 130, 170, 191, 267, 920, 939
StyI	CCWWGG	1 cut(s)	427
TaaI	ACNGT	3 cut(s)	262, 699, 936
TaiI	ACGT	1 cut(s)	328
TaqI	TCGA	4 cut(s)	127, 201, 676, 897
TasI	AATT	1 cut(s)	678
TauI	GCSGC	1 cut(s)	180
TfiI	GAWTC	2 cut(s)	118, 145
Tru1I	TTAA	2 cut(s)	623, 988
Tru9I	TTAA	2 cut(s)	623, 988
TscAI	CASTG	1 cut(s)	962
TseI	GCWGC	5 cut(s)	339, 485, 722, 725, 746
TspDTI	ATGAA	1 cut(s)	621
TspGWI	ACGGA	2 cut(s)	320, 617
TspRI	CASTG	1 cut(s)	962
VpaK11BI	GGWCC	3 cut(s)	10, 134, 423
XmiI	GTMKAC	2 cut(s)	127, 931
XspI	CTAG	1 cut(s)	522
Zsp2I	ATGCAT	1 cut(s)	673

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.