MD12G1052100.v1.1

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006586
Physical Location & Seq
Forward (+)
5900169 .. 5904916
4748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1052100.v1.1.491

Sequence Viewer

Length: 990 bp
ATGGGACTGGTCCGTTATAGCCGTTGGGCTTCGCGCCTCAAAACATTGAATTCCCAATACAAGCCTTTCCTCTCCGCCACCTCCTTCTTTTCCTCCGGCACCACAGCCGGGAATGAGAACCCAGGTCGACCCGGACCGCCTCCGATTCAAGTCGCGCTCACAGAGTCATCCGGGCGCGGCGTCTTCGCCACCCGGAAAATCGAGAACGGCGAGCTCATACACACAGCCAAGCCCGTCCTGTCTCACCCTTCCCTCTCCACCGTCCACCGGGTCTGTTATTTTTGCCTCAGAAAGCTCAGAAACACGGACACCTCCCAACCACAACGTGTTTCGTTTTGCAGCGACGATTGCAAAAAGCAAGCTAAGGGTTTTTATGATGTTGAGATGAGAGCAGATTGGTCAGTTTATGATGACTATTGCAGGTCCCATGGTTTGAAATACCCACTTCTTGTAAAGCGGTTGGCTTGTATGGTCGTGTCGGGAGCTGCACCTGCCAATCTTCTTGACATACTCCAACCTGCTAGCTTATTCGCAGAGATGATTTCAGAGATGGAAGCGGGGTTTGGCTTGCTAAGGAGTACCTTCAGAAACTCAAATATCACGGATGAACAGATGTCATTTTTAACTAAGCAATGGTATATTGGCATACTGGCACGCATTCGCATTAATGCATTTCGAATTGAGTTGGTTGGGGGACTGTATGATGATCTCCTGTCATCAGCAGCAGCATCTATAGATGCTGAAGCTGCTGTTGGGAATGCTGTTTATATCCTTCCATCGTTCTATAATCATGATTGTGATCCAAATGCCCACATTATATGGATAGAGAACACAGATGCAAGATTGAAGGCCCTACGCGATGTGGACGCAGGTGAAGAGCTCCGGATCTGCTACATCGATGCAAGCATGGATCATGATGCCCGGCAGAGTATACTGTCCCAGGGGTTTGGTTTTCAGTGCAGTTGCCTTCGGTGTCTGTCCGGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

330

Amino Acids

36.55

Weight (kDa)

7.02

Isoelectric Point (pI)

52.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 58 - 298 5.7e-14 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 499, 860
Acc36I ACCTGC 4 cut(s) 411, 499, 526, 860
AccB1I GGYRCC 1 cut(s) 98
AccI GTMKAC 2 cut(s) 127, 931
AccII CGCG 4 cut(s) 34, 155, 177, 858
AccIII TCCGGA 1 cut(s) 882
AciI CCGC 5 cut(s) 75, 137, 177, 457, 557
AclWI GGATC 3 cut(s) 794, 893, 918
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 2 cut(s) 568, 762
AcyI GRCGYC 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 1 cut(s) 580
AfiI CCNNNNNNNGG 2 cut(s) 108, 267
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 4 cut(s) 49, 149, 436, 847
AjnI CCWGG 2 cut(s) 121, 939
AjuI GAANNNNNNNTTGG 4 cut(s) 546, 578, 735, 767
AluBI AGCT 7 cut(s) 214, 295, 362, 485, 525, 746, 880
AluI AGCT 7 cut(s) 214, 295, 362, 485, 525, 746, 880
Alw21I GWGCWC 2 cut(s) 216, 882
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 3 cut(s) 794, 893, 918
Aor13HI TCCGGA 1 cut(s) 882
AoxI GGCC 1 cut(s) 849
ApeKI GCWGC 5 cut(s) 339, 485, 722, 725, 746
ApoI RAATTY 1 cut(s) 49
AseI ATTAAT 1 cut(s) 666
AspLEI GCGC 3 cut(s) 36, 157, 177
AspS9I GGNCC 4 cut(s) 10, 134, 423, 850
AsuC2I CCSGG 6 cut(s) 109, 132, 172, 193, 269, 922
AsuHPI GGTGA 2 cut(s) 236, 884
AsuII TTCGAA 1 cut(s) 676
AsuNHI GCTAGC 1 cut(s) 521
AvaII GGWCC 3 cut(s) 10, 134, 423
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 2 cut(s) 216, 882
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 2 cut(s) 216, 882
BbvI GCAGC 5 cut(s) 351, 472, 733, 734, 737
BccI CCATC 2 cut(s) 544, 784
BceAI ACGGC 2 cut(s) 6, 223
BciT130I CCWGG 2 cut(s) 123, 941
BcnI CCSGG 6 cut(s) 109, 132, 172, 193, 269, 922
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 732
BfuAI ACCTGC 4 cut(s) 411, 499, 526, 860
BisI GCNGC 6 cut(s) 178, 340, 486, 723, 726, 747
BlsI GCNGC 6 cut(s) 179, 341, 487, 724, 727, 748
Bme1390I CCNGG 8 cut(s) 109, 123, 132, 172, 193, 269, 922, 941
Bme18I GGWCC 3 cut(s) 10, 134, 423
BmgT120I GGNCC 4 cut(s) 10, 134, 423, 850
BmiI GGNNCC 2 cut(s) 100, 425
BmrFI CCNGG 8 cut(s) 109, 123, 132, 172, 193, 269, 922, 941
BmsI GCATC 5 cut(s) 727, 737, 826, 889, 907
BmtI GCTAGC 1 cut(s) 525
BpiI GAAGAC 1 cut(s) 175
Bpu10I CCTNAGC 2 cut(s) 363, 572
Bpu14I TTCGAA 1 cut(s) 676
BpuMI CCSGG 6 cut(s) 109, 132, 172, 193, 269, 922
Bsa29I ATCGAT 1 cut(s) 897
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 4 cut(s) 121, 427, 939, 940
BsaWI WCCGGW 2 cut(s) 882, 980
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 267
Bse1I ACTGG 2 cut(s) 12, 654
Bse3DI GCAATG 1 cut(s) 638
Bse8I GATNNNNATC 1 cut(s) 798
BseAI TCCGGA 1 cut(s) 882
BseBI CCWGG 2 cut(s) 123, 941
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 4 cut(s) 121, 427, 939, 940
BseGI GGATG 2 cut(s) 167, 610
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 2 cut(s) 108, 267
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 2 cut(s) 301, 310
BseNI ACTGG 2 cut(s) 12, 654
BseXI GCAGC 5 cut(s) 351, 472, 733, 734, 737
BsgI GTGCAG 2 cut(s) 471, 979
Bsh1236I CGCG 4 cut(s) 34, 155, 177, 858
BshFI GGCC 1 cut(s) 851
BshNI GGYRCC 1 cut(s) 98
BshVI ATCGAT 1 cut(s) 897
BsiHKAI GWGCWC 2 cut(s) 216, 882
BsiSI CCGG 9 cut(s) 96, 108, 132, 171, 193, 268, 883, 922, 981
BslFI GGGAC 4 cut(s) 18, 409, 708, 922
BslI CCNNNNNNNGG 2 cut(s) 108, 267
BsmAI GTCTC 1 cut(s) 246
BsmFI GGGAC 4 cut(s) 18, 409, 708, 922
BsmI GAATGC 2 cut(s) 657, 763
BsnI GGCC 1 cut(s) 851
Bsp119I TTCGAA 1 cut(s) 676
Bsp1286I GDGCHC 2 cut(s) 216, 882
Bsp13I TCCGGA 1 cut(s) 882
Bsp143I GATC 4 cut(s) 706, 799, 885, 910
Bsp19I CCATGG 1 cut(s) 427
BspACI CCGC 5 cut(s) 75, 137, 177, 457, 557
BspANI GGCC 1 cut(s) 851
BspCNI CTCAG 2 cut(s) 300, 309
BspDI ATCGAT 1 cut(s) 897
BspEI TCCGGA 1 cut(s) 882
BspFNI CGCG 4 cut(s) 34, 155, 177, 858
BspHI TCATGA 2 cut(s) 790, 913
BspLI GGNNCC 2 cut(s) 100, 425
BspMI ACCTGC 4 cut(s) 411, 499, 526, 860
BspOI GCTAGC 1 cut(s) 525
BspPI GGATC 3 cut(s) 794, 893, 918
BspQI GCTCTTC 1 cut(s) 870
BspT104I TTCGAA 1 cut(s) 676
BspT107I GGYRCC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 638
BsrI ACTGG 2 cut(s) 12, 654
BssECI CCNNGG 4 cut(s) 121, 427, 939, 940
BssMI GATC 4 cut(s) 706, 799, 885, 910
BssNAI GTATAC 1 cut(s) 932
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 427
Bst1107I GTATAC 1 cut(s) 932
Bst2UI CCWGG 2 cut(s) 123, 941
Bst4CI ACNGT 3 cut(s) 262, 699, 936
Bst6I CTCTTC 1 cut(s) 870
BstACI GRCGYC 1 cut(s) 180
BstBI TTCGAA 1 cut(s) 676
BstC8I GCNNGC 6 cut(s) 212, 360, 523, 569, 655, 904
BstDEI CTNAG 5 cut(s) 287, 296, 363, 572, 627
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 2 cut(s) 167, 610
BstFNI CGCG 4 cut(s) 34, 155, 177, 858
BstHHI GCGC 3 cut(s) 36, 157, 177
BstKTI GATC 4 cut(s) 709, 802, 888, 913
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 4 cut(s) 706, 799, 885, 910
BstMWI GCNNNNNNNGC 3 cut(s) 348, 491, 746
BstNI CCWGG 2 cut(s) 123, 941
BstSCI CCNGG 8 cut(s) 107, 121, 130, 170, 191, 267, 920, 939
BstSFI CTRYAG 1 cut(s) 732
BstUI CGCG 4 cut(s) 34, 155, 177, 858
BstV1I GCAGC 5 cut(s) 351, 472, 733, 734, 737
BstV2I GAAGAC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 885
BstXI CCANNNNNNTGG 1 cut(s) 947
BstYI RGATCY 1 cut(s) 885
BstZ17I GTATAC 1 cut(s) 932
Bsu15I ATCGAT 1 cut(s) 897
BsuRI GGCC 1 cut(s) 851
BsuTUI ATCGAT 1 cut(s) 897
BtgI CCRYGG 1 cut(s) 427
BtgZI GCGATG 1 cut(s) 873
BtsCI GGATG 2 cut(s) 167, 610
BtsIMutI CAGTG 1 cut(s) 962
BveI ACCTGC 4 cut(s) 411, 499, 526, 860
Cac8I GCNNGC 6 cut(s) 212, 360, 523, 569, 655, 904
CciI TCATGA 2 cut(s) 790, 913
CfoI GCGC 3 cut(s) 36, 157, 177
Cfr13I GGNCC 4 cut(s) 10, 134, 423, 850
ClaI ATCGAT 1 cut(s) 897
CpoI CGGWCCG 1 cut(s) 134
CseI GACGC 2 cut(s) 169, 875
Csp6I GTAC 1 cut(s) 579
CspI CGGWCCG 1 cut(s) 134
CviAII CATG 4 cut(s) 428, 791, 907, 914
CviQI GTAC 1 cut(s) 579
DdeI CTNAG 5 cut(s) 287, 296, 363, 572, 627
DpnI GATC 4 cut(s) 708, 801, 887, 912
DpnII GATC 4 cut(s) 706, 799, 885, 910
DraIII CACNNNGTG 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 2 cut(s) 214, 880
Eco130I CCWWGG 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 216, 882
Eco47I GGWCC 3 cut(s) 10, 134, 423
Eco53kI GAGCTC 2 cut(s) 214, 880
Eco57I CTGAAG 2 cut(s) 568, 762
EcoICRI GAGCTC 2 cut(s) 214, 880
EcoO109I RGGNCCY 2 cut(s) 423, 850
EcoRI GAATTC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 121, 939
EcoT14I CCWWGG 1 cut(s) 427
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 2 cut(s) 216, 882
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 4 cut(s) 431, 794, 910, 917
FaqI GGGAC 4 cut(s) 18, 409, 708, 922
FatI CATG 4 cut(s) 427, 790, 906, 913
FauI CCCGC 1 cut(s) 550
FblI GTMKAC 2 cut(s) 127, 931
Fnu4HI GCNGC 6 cut(s) 178, 340, 486, 723, 726, 747
FokI GGATG 2 cut(s) 154, 617
FriOI GRGCYC 2 cut(s) 216, 882
Fsp4HI GCNGC 6 cut(s) 178, 340, 486, 723, 726, 747
FspBI CTAG 1 cut(s) 522
GlaI GCGC 3 cut(s) 35, 156, 176
GluI GCNGC 6 cut(s) 178, 340, 486, 723, 726, 747
HaeIII GGCC 1 cut(s) 851
HapII CCGG 9 cut(s) 96, 108, 132, 171, 193, 268, 883, 922, 981
HgaI GACGC 2 cut(s) 169, 875
HhaI GCGC 3 cut(s) 36, 157, 177
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 4 cut(s) 431, 794, 910, 917
Hin6I GCGC 3 cut(s) 34, 155, 175
HinP1I GCGC 3 cut(s) 34, 155, 175
HincII GTYRAC 1 cut(s) 128
HindII GTYRAC 1 cut(s) 128
HinfI GANTC 2 cut(s) 145, 164
HpaII CCGG 9 cut(s) 96, 108, 132, 171, 193, 268, 883, 922, 981
HphI GGTGA 2 cut(s) 236, 884
Hpy166II GTNNAC 4 cut(s) 128, 265, 865, 932
Hpy188I TCNGA 5 cut(s) 144, 290, 299, 547, 587
Hpy188III TCNNGA 6 cut(s) 202, 480, 503, 791, 883, 914
Hpy8I GTNNAC 4 cut(s) 128, 265, 865, 932
Hpy99I CGWCG 1 cut(s) 347
HpyAV CCTTC 6 cut(s) 94, 258, 592, 782, 841, 977
HpyCH4III ACNGT 3 cut(s) 262, 699, 936
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 8 cut(s) 339, 351, 420, 488, 671, 839, 902, 960
HpyF10VI GCNNNNNNNGC 3 cut(s) 348, 491, 746
HpyF3I CTNAG 5 cut(s) 287, 296, 363, 572, 627
HpySE526I ACGT 1 cut(s) 325
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 4 cut(s) 431, 794, 910, 917
HspAI GCGC 3 cut(s) 34, 155, 175
Kpn2I TCCGGA 1 cut(s) 882
Kzo9I GATC 4 cut(s) 706, 799, 885, 910
LguI GCTCTTC 1 cut(s) 870
LmnI GCTCC 2 cut(s) 482, 885
Lsp1109I GCAGC 5 cut(s) 351, 472, 733, 734, 737
LweI GCATC 5 cut(s) 727, 737, 826, 889, 907
MaeI CTAG 1 cut(s) 522
MaeII ACGT 1 cut(s) 325
MalI GATC 4 cut(s) 708, 801, 887, 912
MboI GATC 4 cut(s) 706, 799, 885, 910
MboII GAAGA 3 cut(s) 175, 491, 887
MflI RGATCY 1 cut(s) 885
MhlI GDGCHC 2 cut(s) 216, 882
MluCI AATT 2 cut(s) 49, 678
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 8 cut(s) 47, 80, 91, 103, 150, 263, 296, 322
Mph1103I ATGCAT 1 cut(s) 673
MroI TCCGGA 1 cut(s) 882
MseI TTAA 3 cut(s) 623, 666, 988
MslI CAYNNNNRTG 1 cut(s) 795
MspI CCGG 9 cut(s) 96, 108, 132, 171, 193, 268, 883, 922, 981
MspR9I CCNGG 8 cut(s) 109, 123, 132, 172, 193, 269, 922, 941
Mva1269I GAATGC 2 cut(s) 657, 763
MvaI CCWGG 2 cut(s) 123, 941
MvnI CGCG 4 cut(s) 34, 155, 177, 858
MwoI GCNNNNNNNGC 3 cut(s) 348, 491, 746
NciI CCSGG 6 cut(s) 109, 132, 172, 193, 269, 922
NcoI CCATGG 1 cut(s) 427
NdeII GATC 4 cut(s) 706, 799, 885, 910
NheI GCTAGC 1 cut(s) 521
NlaIII CATG 4 cut(s) 431, 794, 910, 917
NlaIV GGNNCC 2 cut(s) 100, 425
NsiI ATGCAT 1 cut(s) 673
NspV TTCGAA 1 cut(s) 676
PagI TCATGA 2 cut(s) 790, 913
PaqCI CACCTGC 2 cut(s) 499, 860
PasI CCCWGGG 1 cut(s) 940
PciSI GCTCTTC 1 cut(s) 870
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 2 cut(s) 657, 763
PfeI GAWTC 1 cut(s) 145
PkrI GCNGC 6 cut(s) 179, 341, 487, 724, 727, 748
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PpuMI RGGWCCY 1 cut(s) 423
PshBI ATTAAT 1 cut(s) 666
Psp124BI GAGCTC 2 cut(s) 216, 882
Psp5II RGGWCCY 1 cut(s) 423
Psp6I CCWGG 2 cut(s) 121, 939
PspGI CCWGG 2 cut(s) 121, 939
PspN4I GGNNCC 2 cut(s) 100, 425
PspPI GGNCC 4 cut(s) 10, 134, 423, 850
PspPPI RGGWCCY 1 cut(s) 423
PsuI RGATCY 1 cut(s) 885
RsaI GTAC 1 cut(s) 580
RsaNI GTAC 1 cut(s) 579
RseI CAYNNNNRTG 1 cut(s) 795
Rsr2I CGGWCCG 1 cut(s) 134
RsrII CGGWCCG 1 cut(s) 134
SacI GAGCTC 2 cut(s) 216, 882
SalI GTCGAC 1 cut(s) 126
SapI GCTCTTC 1 cut(s) 870
SaqAI TTAA 3 cut(s) 623, 666, 988
SatI GCNGC 6 cut(s) 178, 340, 486, 723, 726, 747
Sau3AI GATC 4 cut(s) 706, 799, 885, 910
Sau96I GGNCC 4 cut(s) 10, 134, 423, 850
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 8 cut(s) 109, 123, 132, 172, 193, 269, 922, 941
SduI GDGCHC 2 cut(s) 216, 882
SfaNI GCATC 5 cut(s) 727, 737, 826, 889, 907
SfcI CTRYAG 1 cut(s) 732
SfuI TTCGAA 1 cut(s) 676
SinI GGWCC 3 cut(s) 10, 134, 423
SmiMI CAYNNNNRTG 1 cut(s) 795
Sse9I AATT 2 cut(s) 49, 678
SsiI CCGC 5 cut(s) 75, 137, 177, 457, 557
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 2 cut(s) 216, 882
StyD4I CCNGG 8 cut(s) 107, 121, 130, 170, 191, 267, 920, 939
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 262, 699, 936
TaiI ACGT 1 cut(s) 328
TaqI TCGA 4 cut(s) 127, 201, 676, 897
TasI AATT 2 cut(s) 49, 678
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 3 cut(s) 623, 666, 988
Tru9I TTAA 3 cut(s) 623, 666, 988
TscAI CASTG 1 cut(s) 962
TseI GCWGC 5 cut(s) 339, 485, 722, 725, 746
TspDTI ATGAA 1 cut(s) 621
TspGWI ACGGA 2 cut(s) 320, 617
TspRI CASTG 1 cut(s) 962
VpaK11BI GGWCC 3 cut(s) 10, 134, 423
VspI ATTAAT 1 cut(s) 666
XapI RAATTY 1 cut(s) 49
XmiI GTMKAC 2 cut(s) 127, 931
XspI CTAG 1 cut(s) 522
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.