FvH4_6g24520

Serine hydrolase involved in the detoxification of formaldehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012329
Physical Location & Seq
Forward (+)
18606091 .. 18611509
5419 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24520.t1

Sequence Viewer

Length: 870 bp
ATGGAGGAGTGTAAGCCCACAGAGATCAGCAGCTCAAAGATGTTCGAAGGGTACAACAAGAGGTACAAGCACTTCAGTACTATTCTCGGCTGCTCCATGACCTTCCACATCTATTTCCCTCCTTCTCCTTCTCCTTCCCACAAATTCCCCGTTCTTTACTGGCTCTCTGGCCTCACCTGTACGGACGAGAATTTCATAATCAAATCAGGAGCTCAGCGTGCTGCCGCAAAGGAGGGTATTGCTTTGATTGCCCCTGATACATCTCCTAGAGGCTTGTCTATTGAAGGAGAAGCAGAGAGCTGGGATTTTGGTGTAGGTGCTGGATTTTATCTCAATGCTACCCAGGAGAAATGGAAGAACTGGCGTATGTATGATTATGTTGTGAAGGAGTTGCCCAAACTTCTAAACGAAAATTTTCCACAGCTTGATACATTGCGGGCATCTATATCTGGTCACTCAATGGGTGGACATGGAGCTTTGACAATCTACCTGAGAAACCTAGACAAATATAAGTCAGTGTCTGCCTTCTCACCAATTGCAAATCCTACCAACTGTCCATGGGGCCAGAAAGCTTTCTCAAATTATCTAGGTGGCAATAAAACTGACTGGGAGGAATATGATGCCACATGCCTAGTTAAGAAGGTGAAGGATGTGTCAGCCACCATTTTAATTGATCAGGGAGAGGATGACAAATTCTTGAATGATCAGTTGCTGCCACACAAGTTTGCAGAGGCATGCAGCAGTGCTAAAGTTCCACTTCTACTGCGTAGCCAGCCCGGTTATGATCACTCATACTACTTCATTTCTACCTTCATAGACGATCACATCAGTCACCATGCTCAAGCTGTTAAACTGCCTCCTCAACCATGA

Protein Analysis

290

Amino Acids

32.44

Weight (kDa)

6.36

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Esterase PF00756 26 - 276 1.2e-61 Putative esterase
Peptidase_S9 PF00326 110 - 266 7e-08 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41530
fragaria_vesca FvH4_6g24520
malus_domestica MD12G1082500.v1.1 MD14G1077300.v1.1
prunus_persica Prupe.7G053400_v2.0.a1 Prupe.7G053400_v2.0.a1
pyrus_communis pycom12g06870 pycom14g06610
rosa_chinensis RchiOBHm_Chr3g0483631
rosa_laevigata RLG00000023263
rosa_multiflora Rmu_co8515891.1_g000001 Rmu_sc0000555.1_g000009
rosa_roxburghii Rroxscaffold_6G00398170
rosa_rugosa Rorug03G0207500
rosa_samantha Rh3AG256000 Rh3BG292700 Rh3CG290400 Rh3DG286300
rosa_wichuraiana Rw3G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 225, 436
AcsI RAATTY 4 cut(s) 143, 190, 412, 692
AcuI CTGAAG 1 cut(s) 58
AfaI GTAC 4 cut(s) 53, 65, 79, 181
AgsI TTSAA 2 cut(s) 284, 700
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 7 cut(s) 33, 212, 300, 424, 476, 572, 845
AluI AGCT 7 cut(s) 33, 212, 300, 424, 476, 572, 845
Alw21I GWGCWC 1 cut(s) 214
AlwNI CAGNNNCTG 2 cut(s) 521, 712
AoxI GGCC 2 cut(s) 169, 562
ApeKI GCWGC 5 cut(s) 30, 90, 221, 712, 738
ApoI RAATTY 4 cut(s) 143, 190, 412, 692
Asp700I GAANNNNTTC 2 cut(s) 414, 572
AspS9I GGNCC 1 cut(s) 562
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 4 cut(s) 166, 522, 655, 824
AsuII TTCGAA 1 cut(s) 45
BanII GRGCYC 1 cut(s) 214
BarI GAAGNNNNNNTAC 2 cut(s) 56, 88
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 5 cut(s) 42, 77, 208, 699, 750
BciT130I CCWGG 1 cut(s) 344
BclI TGATCA 3 cut(s) 673, 703, 784
BcnI CCSGG 1 cut(s) 777
BfaI CTAG 4 cut(s) 267, 500, 587, 632
BisI GCNGC 6 cut(s) 31, 91, 222, 225, 713, 739
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 6 cut(s) 32, 92, 223, 226, 714, 740
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 2 cut(s) 344, 777
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 1 cut(s) 563
BmrFI CCNGG 2 cut(s) 344, 777
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 2 cut(s) 449, 610
BmuI ACTGGG 1 cut(s) 616
Bpu1102I GCTNAGC 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 825
BpuMI CCSGG 1 cut(s) 777
BsaJI CCNNGG 2 cut(s) 342, 557
Bse1I ACTGG 3 cut(s) 164, 365, 611
Bse3DI GCAATG 1 cut(s) 431
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 2 cut(s) 342, 557
BseGI GGATG 2 cut(s) 655, 691
BseMI GCAATG 1 cut(s) 431
BseMII CTCAG 2 cut(s) 227, 482
BseNI ACTGG 3 cut(s) 164, 365, 611
BseRI GAGGAG 2 cut(s) 20, 849
BseXI GCAGC 5 cut(s) 42, 77, 208, 699, 750
BseYI CCCAGC 1 cut(s) 300
BshFI GGCC 2 cut(s) 171, 564
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 1 cut(s) 777
BsnI GGCC 2 cut(s) 171, 564
Bsp119I TTCGAA 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 5 cut(s) 24, 673, 703, 784, 820
Bsp1720I GCTNAGC 1 cut(s) 213
Bsp19I CCATGG 1 cut(s) 557
BspACI CCGC 2 cut(s) 225, 436
BspANI GGCC 2 cut(s) 171, 564
BspCNI CTCAG 2 cut(s) 226, 483
BspLI GGNNCC 1 cut(s) 563
BspT104I TTCGAA 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 431
BsrI ACTGG 3 cut(s) 164, 365, 611
BssECI CCNNGG 2 cut(s) 342, 557
BssMI GATC 5 cut(s) 24, 673, 703, 784, 820
BssT1I CCWWGG 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 45
BstC8I GCNNGC 4 cut(s) 219, 438, 736, 773
BstDEI CTNAG 2 cut(s) 213, 491
BstDSI CCRYGG 1 cut(s) 557
BstF5I GGATG 2 cut(s) 655, 691
BstKTI GATC 5 cut(s) 27, 676, 706, 787, 823
BstMBI GATC 5 cut(s) 24, 673, 703, 784, 820
BstMWI GCNNNNNNNGC 3 cut(s) 218, 248, 772
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 2 cut(s) 630, 738
BstSCI CCNGG 2 cut(s) 342, 775
BstV1I GCAGC 5 cut(s) 42, 77, 208, 699, 750
BsuRI GGCC 2 cut(s) 171, 564
BtgI CCRYGG 1 cut(s) 557
BtsCI GGATG 2 cut(s) 655, 691
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 2 cut(s) 522, 748
Cac8I GCNNGC 4 cut(s) 219, 438, 736, 773
CaiI CAGNNNCTG 2 cut(s) 521, 712
Cfr13I GGNCC 1 cut(s) 562
Csp6I GTAC 4 cut(s) 52, 64, 78, 180
CviAII CATG 7 cut(s) 97, 470, 558, 627, 735, 836, 867
CviQI GTAC 4 cut(s) 52, 64, 78, 180
DdeI CTNAG 2 cut(s) 213, 491
DpnI GATC 5 cut(s) 26, 675, 705, 786, 822
DpnII GATC 5 cut(s) 24, 673, 703, 784, 820
Ecl136II GAGCTC 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 557
Eco24I GRGCYC 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 557
EcoT38I GRGCYC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 7 cut(s) 100, 473, 561, 630, 738, 839, 870
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FatI CATG 7 cut(s) 96, 469, 557, 626, 734, 835, 866
FauI CCCGC 1 cut(s) 429
FbaI TGATCA 3 cut(s) 673, 703, 784
Fnu4HI GCNGC 6 cut(s) 31, 91, 222, 225, 713, 739
FokI GGATG 2 cut(s) 662, 698
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 6 cut(s) 31, 91, 222, 225, 713, 739
FspBI CTAG 4 cut(s) 267, 500, 587, 632
GluI GCNGC 6 cut(s) 31, 91, 222, 225, 713, 739
GsaI CCCAGC 1 cut(s) 304
HaeIII GGCC 2 cut(s) 171, 564
HapII CCGG 1 cut(s) 777
Hin1II CATG 7 cut(s) 100, 473, 561, 630, 738, 839, 870
HindIII AAGCTT 1 cut(s) 570
HpaII CCGG 1 cut(s) 777
HphI GGTGA 4 cut(s) 166, 522, 655, 824
Hpy166II GTNNAC 1 cut(s) 467
Hpy188III TCNNGA 2 cut(s) 207, 697
Hpy8I GTNNAC 1 cut(s) 467
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4V TGCA 3 cut(s) 539, 728, 738
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 248, 772
HpyF3I CTNAG 2 cut(s) 213, 491
Hsp92II CATG 7 cut(s) 100, 473, 561, 630, 738, 839, 870
Ksp22I TGATCA 3 cut(s) 673, 703, 784
Kzo9I GATC 5 cut(s) 24, 673, 703, 784, 820
LmnI GCTCC 3 cut(s) 98, 209, 473
Lsp1109I GCAGC 5 cut(s) 42, 77, 208, 699, 750
LweI GCATC 2 cut(s) 449, 610
MaeI CTAG 4 cut(s) 267, 500, 587, 632
MaeIII GTNAC 2 cut(s) 452, 830
MalI GATC 5 cut(s) 26, 675, 705, 786, 822
MboI GATC 5 cut(s) 24, 673, 703, 784, 820
MboII GAAGA 1 cut(s) 367
MfeI CAATTG 1 cut(s) 534
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 7 cut(s) 143, 190, 412, 534, 580, 669, 692
MroXI GAANNNNTTC 2 cut(s) 414, 572
MseI TTAA 3 cut(s) 636, 668, 849
MspI CCGG 1 cut(s) 777
MspR9I CCNGG 2 cut(s) 344, 777
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 344
MwoI GCNNNNNNNGC 3 cut(s) 218, 248, 772
NciI CCSGG 1 cut(s) 777
NcoI CCATGG 1 cut(s) 557
NdeII GATC 5 cut(s) 24, 673, 703, 784, 820
NlaIII CATG 7 cut(s) 100, 473, 561, 630, 738, 839, 870
NlaIV GGNNCC 1 cut(s) 563
NmeAIII GCCGAG 1 cut(s) 66
NmuCI GTSAC 2 cut(s) 452, 830
NspI RCATGY 2 cut(s) 630, 738
NspV TTCGAA 1 cut(s) 45
PaeI GCATGC 1 cut(s) 738
PdmI GAANNNNTTC 2 cut(s) 414, 572
PkrI GCNGC 6 cut(s) 32, 92, 223, 226, 714, 740
Psp124BI GAGCTC 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 342
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 563
PspPI GGNCC 1 cut(s) 562
PstNI CAGNNNCTG 2 cut(s) 521, 712
RsaI GTAC 4 cut(s) 53, 65, 79, 181
RsaNI GTAC 4 cut(s) 52, 64, 78, 180
SacI GAGCTC 1 cut(s) 214
SaqAI TTAA 3 cut(s) 636, 668, 849
SatI GCNGC 6 cut(s) 31, 91, 222, 225, 713, 739
Sau3AI GATC 5 cut(s) 24, 673, 703, 784, 820
Sau96I GGNCC 1 cut(s) 562
ScaI AGTACT 1 cut(s) 79
ScrFI CCNGG 2 cut(s) 344, 777
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 2 cut(s) 449, 610
SfuI TTCGAA 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 840
SmoI CTYRAG 1 cut(s) 840
SphI GCATGC 1 cut(s) 738
Sse9I AATT 7 cut(s) 143, 190, 412, 534, 580, 669, 692
SsiI CCGC 2 cut(s) 225, 436
SspMI CTAG 4 cut(s) 267, 500, 587, 632
SstI GAGCTC 1 cut(s) 214
StyD4I CCNGG 2 cut(s) 342, 775
StyI CCWWGG 1 cut(s) 557
TaaI ACNGT 1 cut(s) 554
TaqI TCGA 1 cut(s) 45
TasI AATT 7 cut(s) 143, 190, 412, 534, 580, 669, 692
TatI WGTACW 1 cut(s) 77
TauI GCSGC 1 cut(s) 227
Tru1I TTAA 3 cut(s) 636, 668, 849
Tru9I TTAA 3 cut(s) 636, 668, 849
TscAI CASTG 2 cut(s) 522, 748
TseFI GTSAC 2 cut(s) 452, 830
TseI GCWGC 5 cut(s) 30, 90, 221, 712, 738
Tsp45I GTSAC 2 cut(s) 452, 830
TspDTI ATGAA 3 cut(s) 184, 790, 802
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 2 cut(s) 522, 748
XapI RAATTY 4 cut(s) 143, 190, 412, 692
XceI RCATGY 2 cut(s) 630, 738
XmnI GAANNNNTTC 2 cut(s) 414, 572
XspI CTAG 4 cut(s) 267, 500, 587, 632
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.