Rh3CG290400

Serine hydrolase involved in the detoxification of formaldehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0012329
Physical Location & Seq
Forward (+)
29448177 .. 29453695
5519 bp
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UTR
Exon/CDS
Intron
Rh3CG290400.1

Sequence Viewer

Length: 684 bp
ATGGAGGAGTGTAAGCCCACTGAGATCAGCAGCTCAAAGATGTTTGGAGGGTACAACAAGAGATACAAGCATTTCAGTCCTACACTGGGCTGCTCCATGACCTTCCACATCTATTTTCCTCCTTCTCCTTCCCCTTCCCACAAATTCCCCGTTCTTTACTGGCTCTCTGGCCTTACCTGCACAGATGAGAATTTTATAACCAAATCAGGCGCTCAGCGTTCTGCAGCAAAGGAGGGTATTGCTTTGATTGCCCCTGATACATCTCCTAGAGGCTTGTCTATTGAAGGAGAAGCAGATAGCTGGGATTTTGGTGTAGGTGCTGGATTTTATCTCAATGCTACCCAGGAGAAATGGAAGAACTGGCGTATGTATGATTATGTTGTCAAGGAGTTACCCAAACTTCTGAATGAAAATTTTCCACAGCTTGATACATTCCGGGCATCTATATCTGGTCACTCAATGGGTGGACATGGTGCTTTGACAATCTACCTGAAAAACCTAGACAAATATAAGTCAGTATCTGCCTTCTCACCAATTGTGAATCCTATCAACTGCCCATGGGGTCAGAAAGCTTTCTCAAATTATCTAGGTGGCAGTAAAGCTGACTGGGAGGAATATGATGCCACTTGCCTAGTTAAGAAGTTTAATGATGTGTCAGCCACCATTTTAATTGATCAGGTATGA

Protein Analysis

227

Amino Acids

25.34

Weight (kDa)

6.95

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Esterase PF00756 26 - 214 3.3e-51 Putative esterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41530
fragaria_vesca FvH4_6g24520
malus_domestica MD12G1082500.v1.1 MD14G1077300.v1.1
prunus_persica Prupe.7G053400_v2.0.a1 Prupe.7G053400_v2.0.a1
pyrus_communis pycom12g06870 pycom14g06610
rosa_chinensis RchiOBHm_Chr3g0483631
rosa_laevigata RLG00000023263
rosa_multiflora Rmu_co8515891.1_g000001 Rmu_sc0000555.1_g000009
rosa_roxburghii Rroxscaffold_6G00398170
rosa_rugosa Rorug03G0207500
rosa_samantha Rh3AG256000 Rh3BG292700 Rh3CG290400 Rh3DG286300
rosa_wichuraiana Rw3G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 185
AcsI RAATTY 3 cut(s) 143, 190, 412
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 5 cut(s) 33, 300, 424, 572, 602
AluI AGCT 5 cut(s) 33, 300, 424, 572, 602
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 3 cut(s) 30, 90, 224
ApoI RAATTY 3 cut(s) 143, 190, 412
Asp700I GAANNNNTTC 2 cut(s) 414, 572
AspLEI GCGC 1 cut(s) 212
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 1 cut(s) 522
BbvI GCAGC 3 cut(s) 42, 77, 236
BciT130I CCWGG 1 cut(s) 344
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 1 cut(s) 437
BfaI CTAG 4 cut(s) 267, 500, 587, 632
BfmI CTRYAG 1 cut(s) 222
BfoI RGCGCY 1 cut(s) 213
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 3 cut(s) 31, 91, 225
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 3 cut(s) 32, 92, 226
Bme1390I CCNGG 2 cut(s) 344, 437
BmrFI CCNGG 2 cut(s) 344, 437
BmrI ACTGGG 2 cut(s) 95, 616
BmsI GCATC 2 cut(s) 449, 610
BmuI ACTGGG 2 cut(s) 95, 616
Bpu1102I GCTNAGC 1 cut(s) 213
BpuMI CCSGG 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 342, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 4 cut(s) 90, 164, 365, 611
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 2 cut(s) 342, 557
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 12, 227
BseNI ACTGG 4 cut(s) 90, 164, 365, 611
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 42, 77, 236
BseYI CCCAGC 1 cut(s) 300
BsgI GTGCAG 1 cut(s) 163
BshFI GGCC 1 cut(s) 171
BsiSI CCGG 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 86
BsnI GGCC 1 cut(s) 171
Bsp143I GATC 2 cut(s) 24, 673
Bsp1720I GCTNAGC 1 cut(s) 213
Bsp19I CCATGG 1 cut(s) 557
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 2 cut(s) 13, 226
BspMAI CTGCAG 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 185
BsrI ACTGG 4 cut(s) 90, 164, 365, 611
BssECI CCNNGG 2 cut(s) 342, 557
BssMI GATC 2 cut(s) 24, 673
BssT1I CCWWGG 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 344
BstDEI CTNAG 2 cut(s) 21, 213
BstDSI CCRYGG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 2 cut(s) 27, 676
BstMBI GATC 2 cut(s) 24, 673
BstMWI GCNNNNNNNGC 2 cut(s) 177, 248
BstNI CCWGG 1 cut(s) 344
BstSCI CCNGG 2 cut(s) 342, 435
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 3 cut(s) 42, 77, 236
BsuRI GGCC 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 557
BtsIMutI CAGTG 2 cut(s) 18, 83
BveI ACCTGC 1 cut(s) 185
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 52
CviAII CATG 3 cut(s) 97, 470, 558
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 2 cut(s) 21, 213
DpnI GATC 2 cut(s) 26, 675
DpnII GATC 2 cut(s) 24, 673
Eco130I CCWWGG 1 cut(s) 557
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 557
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 3 cut(s) 100, 473, 561
FatI CATG 3 cut(s) 96, 469, 557
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 3 cut(s) 31, 91, 225
Fsp4HI GCNGC 3 cut(s) 31, 91, 225
FspBI CTAG 4 cut(s) 267, 500, 587, 632
GlaI GCGC 1 cut(s) 211
GluI GCNGC 3 cut(s) 31, 91, 225
GsaI CCCAGC 1 cut(s) 304
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 171
HapII CCGG 1 cut(s) 436
HhaI GCGC 1 cut(s) 212
Hin1II CATG 3 cut(s) 100, 473, 561
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 570
HinfI GANTC 1 cut(s) 541
HpaII CCGG 1 cut(s) 436
HphI GGTGA 1 cut(s) 522
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 2 cut(s) 405, 567
Hpy8I GTNNAC 1 cut(s) 467
HpyAV CCTTC 6 cut(s) 112, 132, 138, 144, 278, 535
HpyCH4V TGCA 2 cut(s) 180, 224
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 248
HpyF3I CTNAG 2 cut(s) 21, 213
Hsp92II CATG 3 cut(s) 100, 473, 561
HspAI GCGC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 2 cut(s) 24, 673
LmnI GCTCC 1 cut(s) 98
Lsp1109I GCAGC 3 cut(s) 42, 77, 236
LweI GCATC 2 cut(s) 449, 610
MaeI CTAG 4 cut(s) 267, 500, 587, 632
MaeIII GTNAC 2 cut(s) 390, 452
MalI GATC 2 cut(s) 26, 675
MboI GATC 2 cut(s) 24, 673
MboII GAAGA 1 cut(s) 367
MfeI CAATTG 1 cut(s) 534
MluCI AATT 6 cut(s) 143, 190, 412, 534, 580, 669
MnlI CCTC 5 cut(s) 41, 129, 226, 263, 604
MroXI GAANNNNTTC 2 cut(s) 414, 572
MseI TTAA 3 cut(s) 636, 645, 668
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 2 cut(s) 344, 437
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 344
MwoI GCNNNNNNNGC 2 cut(s) 177, 248
NciI CCSGG 1 cut(s) 437
NcoI CCATGG 1 cut(s) 557
NdeII GATC 2 cut(s) 24, 673
NlaIII CATG 3 cut(s) 100, 473, 561
NmuCI GTSAC 1 cut(s) 452
PdmI GAANNNNTTC 2 cut(s) 414, 572
PfeI GAWTC 1 cut(s) 541
PkrI GCNGC 3 cut(s) 32, 92, 226
PsiI TTATAA 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 342
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 1 cut(s) 342
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 521
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
SaqAI TTAA 3 cut(s) 636, 645, 668
SatI GCNGC 3 cut(s) 31, 91, 225
Sau3AI GATC 2 cut(s) 24, 673
ScrFI CCNGG 2 cut(s) 344, 437
SfaNI GCATC 2 cut(s) 449, 610
SfcI CTRYAG 1 cut(s) 222
Sse9I AATT 6 cut(s) 143, 190, 412, 534, 580, 669
SspMI CTAG 4 cut(s) 267, 500, 587, 632
StyD4I CCNGG 2 cut(s) 342, 435
StyI CCWWGG 1 cut(s) 557
TasI AATT 6 cut(s) 143, 190, 412, 534, 580, 669
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 3 cut(s) 636, 645, 668
Tru9I TTAA 3 cut(s) 636, 645, 668
TscAI CASTG 2 cut(s) 25, 90
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 3 cut(s) 30, 90, 224
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 1 cut(s) 423
TspRI CASTG 2 cut(s) 25, 90
XapI RAATTY 3 cut(s) 143, 190, 412
XmnI GAANNNNTTC 2 cut(s) 414, 572
XspI CTAG 4 cut(s) 267, 500, 587, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.