RLG00000023263

Serine hydrolase involved in the detoxification of formaldehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012329
Physical Location & Seq
Reverse (-)
20854145 .. 20859372
5228 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023263

Sequence Viewer

Length: 804 bp
ATGGAGGAGTGTAAGCCCACTGAGATCAGCAGCTCAAAGATGTTTGGAGGGTACAACAAGAGATACAAGCATTTCAGTCCTACACTGGGCTGCTCCATGACCTTCCACATCTATTTTCCTCCTTCTCCTTCCCCTTCCCACAAATTCCCCGTTCTTTACTGGCTCTCTGGCCTTACCTGCACAGATGAGAATTTTATAAGCAAATCAGGCGCTCAGCGTTCTGCAGCAAAGGAGGGTATTGCTTTGATTGCCCCTGATACATCTCCTAGAGGCTTGTCTATTGAAGGAGAAGCAGATAGCTGGGATTTTGGTGTAGGTGCTGGATTTTATCTCAATGCTACCCAGGAGAAATGGAAGAACTGGCGTATGTATGATTATGTTGTCAAGGAGTTACCCAAACTTCTGAATGAAAATTTTCCACAGCTTGATACATTGCGGGCATCTATATCTGGTCACTCAATGGGTGGACATGGTGCTTTGACAATCTACCTGAAAAACCTAGACAAATATAAGTCAGTATCTGCCTTCTCACCAATTGTGAATCCTATCAACTGCCCATGGGGTCAGAAAGCTTTCTCAAATTATCTAGGTGGCAGTAAAGCTGACTGGGAGGGGGAGGATGACAAATTCTTGCATGATCAGCTACTACCACATAAGTTTGCAGAGGCATGCAGCAGTGCTAAAGTTCCACTTCTACTGCGTACCCAGCCTGGTTATGATCACTCATACTATTTCATTGCTACCTTCATAGACGATCACATCAGTCACCATGCTCAAGCTGTTAAACTGCCTACACCACCATGA

Protein Analysis

268

Amino Acids

29.84

Weight (kDa)

6.82

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Esterase PF00756 26 - 254 1.2e-51 Putative esterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41530
fragaria_vesca FvH4_6g24520
malus_domestica MD12G1082500.v1.1 MD14G1077300.v1.1
prunus_persica Prupe.7G053400_v2.0.a1 Prupe.7G053400_v2.0.a1
pyrus_communis pycom12g06870 pycom14g06610
rosa_chinensis RchiOBHm_Chr3g0483631
rosa_laevigata RLG00000023263
rosa_multiflora Rmu_co8515891.1_g000001 Rmu_sc0000555.1_g000009
rosa_roxburghii Rroxscaffold_6G00398170
rosa_rugosa Rorug03G0207500
rosa_samantha Rh3AG256000 Rh3BG292700 Rh3CG290400 Rh3DG286300
rosa_wichuraiana Rw3G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 185
AciI CCGC 1 cut(s) 436
AcsI RAATTY 4 cut(s) 143, 190, 412, 626
AfaI GTAC 2 cut(s) 53, 703
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 2 cut(s) 342, 709
AluBI AGCT 7 cut(s) 33, 300, 424, 572, 602, 643, 779
AluI AGCT 7 cut(s) 33, 300, 424, 572, 602, 643, 779
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 4 cut(s) 30, 90, 224, 672
ApoI RAATTY 4 cut(s) 143, 190, 412, 626
Asp700I GAANNNNTTC 2 cut(s) 414, 572
AspLEI GCGC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 522, 758
BbvI GCAGC 4 cut(s) 42, 77, 236, 684
BciT130I CCWGG 2 cut(s) 344, 711
BclI TGATCA 2 cut(s) 637, 718
BfaI CTAG 3 cut(s) 267, 500, 587
BfmI CTRYAG 1 cut(s) 222
BfoI RGCGCY 1 cut(s) 213
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 4 cut(s) 31, 91, 225, 673
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 4 cut(s) 32, 92, 226, 674
Bme1390I CCNGG 2 cut(s) 344, 711
BmrFI CCNGG 2 cut(s) 344, 711
BmrI ACTGGG 2 cut(s) 95, 616
BmsI GCATC 1 cut(s) 449
BmuI ACTGGG 2 cut(s) 95, 616
Bpu1102I GCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 759
BsaJI CCNNGG 2 cut(s) 342, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 4 cut(s) 90, 164, 365, 611
Bse3DI GCAATG 2 cut(s) 431, 735
BseBI CCWGG 2 cut(s) 344, 711
BseDI CCNNGG 2 cut(s) 342, 557
BseGI GGATG 1 cut(s) 625
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 2 cut(s) 431, 735
BseMII CTCAG 2 cut(s) 12, 227
BseNI ACTGG 4 cut(s) 90, 164, 365, 611
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 42, 77, 236, 684
BseYI CCCAGC 2 cut(s) 300, 705
BsgI GTGCAG 1 cut(s) 163
BshFI GGCC 1 cut(s) 171
BslI CCNNNNNNNGG 1 cut(s) 86
BsnI GGCC 1 cut(s) 171
Bsp143I GATC 4 cut(s) 24, 637, 718, 754
Bsp1720I GCTNAGC 1 cut(s) 213
Bsp19I CCATGG 1 cut(s) 557
BspACI CCGC 1 cut(s) 436
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 2 cut(s) 13, 226
BspMAI CTGCAG 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 431, 735
BsrI ACTGG 4 cut(s) 90, 164, 365, 611
BssECI CCNNGG 2 cut(s) 342, 557
BssMI GATC 4 cut(s) 24, 637, 718, 754
BssT1I CCWWGG 1 cut(s) 557
Bst2UI CCWGG 2 cut(s) 344, 711
BstC8I GCNNGC 2 cut(s) 438, 670
BstDEI CTNAG 2 cut(s) 21, 213
BstDSI CCRYGG 1 cut(s) 557
BstF5I GGATG 1 cut(s) 625
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 4 cut(s) 27, 640, 721, 757
BstMBI GATC 4 cut(s) 24, 637, 718, 754
BstMWI GCNNNNNNNGC 5 cut(s) 177, 207, 248, 640, 706
BstNI CCWGG 2 cut(s) 344, 711
BstNSI RCATGY 1 cut(s) 672
BstSCI CCNGG 2 cut(s) 342, 709
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 42, 77, 236, 684
BsuRI GGCC 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 557
BtsCI GGATG 1 cut(s) 625
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 3 cut(s) 18, 83, 682
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 2 cut(s) 438, 670
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 1 cut(s) 212
Csp6I GTAC 2 cut(s) 52, 702
CviAII CATG 7 cut(s) 97, 470, 558, 635, 669, 770, 801
CviQI GTAC 2 cut(s) 52, 702
DdeI CTNAG 2 cut(s) 21, 213
DpnI GATC 4 cut(s) 26, 639, 720, 756
DpnII GATC 4 cut(s) 24, 637, 718, 754
Eco130I CCWWGG 1 cut(s) 557
EcoRII CCWGG 2 cut(s) 342, 709
EcoT14I CCWWGG 1 cut(s) 557
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 7 cut(s) 100, 473, 561, 638, 672, 773, 804
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FatI CATG 7 cut(s) 96, 469, 557, 634, 668, 769, 800
FauI CCCGC 1 cut(s) 429
FbaI TGATCA 2 cut(s) 637, 718
Fnu4HI GCNGC 4 cut(s) 31, 91, 225, 673
FokI GGATG 1 cut(s) 632
Fsp4HI GCNGC 4 cut(s) 31, 91, 225, 673
FspBI CTAG 3 cut(s) 267, 500, 587
GlaI GCGC 1 cut(s) 211
GluI GCNGC 4 cut(s) 31, 91, 225, 673
GsaI CCCAGC 2 cut(s) 304, 709
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 171
HhaI GCGC 1 cut(s) 212
Hin1II CATG 7 cut(s) 100, 473, 561, 638, 672, 773, 804
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 570
HinfI GANTC 1 cut(s) 541
HphI GGTGA 2 cut(s) 522, 758
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 2 cut(s) 405, 567
Hpy8I GTNNAC 1 cut(s) 467
HpyAV CCTTC 7 cut(s) 112, 132, 138, 144, 278, 535, 754
HpyCH4V TGCA 5 cut(s) 180, 224, 634, 662, 672
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 207, 248, 640, 706
HpyF3I CTNAG 2 cut(s) 21, 213
Hsp92II CATG 7 cut(s) 100, 473, 561, 638, 672, 773, 804
HspAI GCGC 1 cut(s) 210
Ksp22I TGATCA 2 cut(s) 637, 718
Kzo9I GATC 4 cut(s) 24, 637, 718, 754
LmnI GCTCC 1 cut(s) 98
Lsp1109I GCAGC 4 cut(s) 42, 77, 236, 684
LweI GCATC 1 cut(s) 449
MaeI CTAG 3 cut(s) 267, 500, 587
MaeIII GTNAC 3 cut(s) 390, 452, 764
MalI GATC 4 cut(s) 26, 639, 720, 756
MboI GATC 4 cut(s) 24, 637, 718, 754
MboII GAAGA 1 cut(s) 367
MfeI CAATTG 1 cut(s) 534
MluCI AATT 6 cut(s) 143, 190, 412, 534, 580, 626
MnlI CCTC 7 cut(s) 41, 129, 226, 263, 604, 610, 658
MroXI GAANNNNTTC 2 cut(s) 414, 572
MseI TTAA 1 cut(s) 783
MslI CAYNNNNRTG 1 cut(s) 799
MspR9I CCNGG 2 cut(s) 344, 711
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 2 cut(s) 344, 711
MwoI GCNNNNNNNGC 5 cut(s) 177, 207, 248, 640, 706
NcoI CCATGG 1 cut(s) 557
NdeII GATC 4 cut(s) 24, 637, 718, 754
NlaIII CATG 7 cut(s) 100, 473, 561, 638, 672, 773, 804
NmuCI GTSAC 2 cut(s) 452, 764
NspI RCATGY 1 cut(s) 672
PaeI GCATGC 1 cut(s) 672
PdmI GAANNNNTTC 2 cut(s) 414, 572
PfeI GAWTC 1 cut(s) 541
PkrI GCNGC 4 cut(s) 32, 92, 226, 674
PsiI TTATAA 1 cut(s) 197
Psp6I CCWGG 2 cut(s) 342, 709
PspFI CCCAGC 2 cut(s) 300, 705
PspGI CCWGG 2 cut(s) 342, 709
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 521
RsaI GTAC 2 cut(s) 53, 703
RsaNI GTAC 2 cut(s) 52, 702
RseI CAYNNNNRTG 1 cut(s) 799
SaqAI TTAA 1 cut(s) 783
SatI GCNGC 4 cut(s) 31, 91, 225, 673
Sau3AI GATC 4 cut(s) 24, 637, 718, 754
ScrFI CCNGG 2 cut(s) 344, 711
SfaNI GCATC 1 cut(s) 449
SfcI CTRYAG 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 799
SmlI CTYRAG 1 cut(s) 774
SmoI CTYRAG 1 cut(s) 774
SphI GCATGC 1 cut(s) 672
Sse9I AATT 6 cut(s) 143, 190, 412, 534, 580, 626
SsiI CCGC 1 cut(s) 436
SspMI CTAG 3 cut(s) 267, 500, 587
StyD4I CCNGG 2 cut(s) 342, 709
StyI CCWWGG 1 cut(s) 557
TasI AATT 6 cut(s) 143, 190, 412, 534, 580, 626
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 1 cut(s) 783
Tru9I TTAA 1 cut(s) 783
TscAI CASTG 3 cut(s) 25, 90, 682
TseFI GTSAC 2 cut(s) 452, 764
TseI GCWGC 4 cut(s) 30, 90, 224, 672
Tsp45I GTSAC 2 cut(s) 452, 764
TspDTI ATGAA 3 cut(s) 423, 724, 736
TspRI CASTG 3 cut(s) 25, 90, 682
XapI RAATTY 4 cut(s) 143, 190, 412, 626
XceI RCATGY 1 cut(s) 672
XmnI GAANNNNTTC 2 cut(s) 414, 572
XspI CTAG 3 cut(s) 267, 500, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.