MD14G1077300.v1.1

Serine hydrolase involved in the detoxification of formaldehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012329
Physical Location & Seq
Forward (+)
8778374 .. 8782606
4233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1077300.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGAGACGAAGCCAAGTGAGATGAGCAGCTCCAAGATGTTCGGAGGGTACAACAAGAGGTACAAGCATTTCAGCCCTACGCTCGGCTGCTCCATGACCTTCCACATCTACTTCCCTCCTTCCCCTTCCCCTTCCCACCGATTCCCTGTAATTTACTGGCTCTCTGGCCTGACCTGCACGGATGAAAATTTTATAACCAAGTCAGGAGCTCAACGCGTTGCCTCAAGTGAGGGTGTTGCTCTGATCGCCCTTGATACTTCTCCAAGAGGCTTGTCTATAGAAGGAGAGGCGGATAGCTGGGATTTTGGTGTAGGTGCTGGGTTTTATCTCAATGCTACTGAAGAGAAATGGAAGAACTGGCGCATGTACGATTATGTTGTCAAGGAGCTGCCACAACTTCTGAATGACAACTTTTCACAGCTTGATACATCGCGAGCATCTATATCTGGTCATTCTATGGGTGGGCACGGTGCTTTGACAATCTACCTGAAAAACCTGGATAAATATAAGTCAGTATCTGCCTTTGCACCCATTGTGAATCCTATTAACTGTCCATGGGGCCAGAAAGCTTTCTCAAATTATCTAGGTGGCAATAAAACTGATTGGGAAGAATATGATGCCACTTCCCTAATTAAGAAGTTTAATGATGTTTCAGGTACCATTTTAATTGATCAGGGGGAGGATGACAAATTCTTGCATGATCAGTTGATGCCACACAAGTTTGAAGAGGCATGTCAGAATGCTAAAGTTCCACTCCTTCTGCGACTGCAGCCCGGTTATGATCACTCATACTTTTTCATTTCCACCTTCATCGATGATCACATCCGTCACCATGCTCAAGCCCTTAATCTACCGTGA

Protein Analysis

286

Amino Acids

32.12

Weight (kDa)

5.98

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Esterase PF00756 25 - 276 3e-61 Putative esterase
Peptidase_S9 PF00326 109 - 264 3.5e-10 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41530
fragaria_vesca FvH4_6g24520
malus_domestica MD12G1082500.v1.1 MD14G1077300.v1.1
prunus_persica Prupe.7G053400_v2.0.a1 Prupe.7G053400_v2.0.a1
pyrus_communis pycom12g06870 pycom14g06610
rosa_chinensis RchiOBHm_Chr3g0483631
rosa_laevigata RLG00000023263
rosa_multiflora Rmu_co8515891.1_g000001 Rmu_sc0000555.1_g000009
rosa_roxburghii Rroxscaffold_6G00398170
rosa_rugosa Rorug03G0207500
rosa_samantha Rh3AG256000 Rh3BG292700 Rh3CG290400 Rh3DG286300
rosa_wichuraiana Rw3G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 182
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 656
AccII CGCG 2 cut(s) 216, 433
AciI CCGC 1 cut(s) 290
AcsI RAATTY 2 cut(s) 187, 689
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 4 cut(s) 50, 62, 368, 658
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 1 cut(s) 725
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 6 cut(s) 30, 209, 297, 388, 421, 569
AluI AGCT 6 cut(s) 30, 209, 297, 388, 421, 569
Alw21I GWGCWC 1 cut(s) 211
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 2 cut(s) 166, 559
ApeKI GCWGC 4 cut(s) 27, 87, 388, 769
ApoI RAATTY 2 cut(s) 187, 689
Asp700I GAANNNNTTC 1 cut(s) 569
Asp718I GGTACC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 559
AsuC2I CCSGG 1 cut(s) 774
AsuHPI GGTGA 1 cut(s) 821
BaeGI GKGCMC 1 cut(s) 468
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 4 cut(s) 39, 74, 375, 781
BciT130I CCWGG 1 cut(s) 497
BclI TGATCA 4 cut(s) 670, 700, 781, 817
BcnI CCSGG 1 cut(s) 774
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 2 cut(s) 276, 767
BfuAI ACCTGC 1 cut(s) 182
BisI GCNGC 4 cut(s) 28, 88, 389, 770
BlsI GCNGC 4 cut(s) 29, 89, 390, 771
Bme1390I CCNGG 2 cut(s) 497, 774
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 2 cut(s) 560, 658
BmrFI CCNGG 2 cut(s) 497, 774
BmsI GCATC 3 cut(s) 446, 607, 699
BpuEI CTTGAG 2 cut(s) 208, 822
BpuMI CCSGG 1 cut(s) 774
Bsa29I ATCGAT 1 cut(s) 813
BsaJI CCNNGG 1 cut(s) 554
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 161, 362
BseBI CCWGG 1 cut(s) 497
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 1 cut(s) 554
BseGI GGATG 3 cut(s) 187, 688, 822
BseLI CCNNNNNNNGG 1 cut(s) 83
BseNI ACTGG 2 cut(s) 161, 362
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 4 cut(s) 39, 74, 375, 781
BseYI CCCAGC 2 cut(s) 297, 317
BsgI GTGCAG 1 cut(s) 160
Bsh1236I CGCG 2 cut(s) 216, 433
BshFI GGCC 2 cut(s) 168, 561
BshNI GGYRCC 1 cut(s) 656
BshVI ATCGAT 1 cut(s) 813
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 1 cut(s) 774
BslI CCNNNNNNNGG 1 cut(s) 83
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 2 cut(s) 168, 561
Bsp1286I GDGCHC 2 cut(s) 211, 468
Bsp143I GATC 5 cut(s) 243, 670, 700, 781, 817
Bsp19I CCATGG 1 cut(s) 554
Bsp68I TCGCGA 1 cut(s) 433
BspACI CCGC 1 cut(s) 290
BspANI GGCC 2 cut(s) 168, 561
BspDI ATCGAT 1 cut(s) 813
BspFNI CGCG 2 cut(s) 216, 433
BspLI GGNNCC 2 cut(s) 560, 658
BspMAI CTGCAG 1 cut(s) 771
BspMI ACCTGC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 656
BsrI ACTGG 2 cut(s) 161, 362
BssECI CCNNGG 1 cut(s) 554
BssMI GATC 5 cut(s) 243, 670, 700, 781, 817
BssT1I CCWWGG 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 3 cut(s) 470, 551, 855
Bst6I CTCTTC 2 cut(s) 336, 720
BstC8I GCNNGC 1 cut(s) 435
BstDSI CCRYGG 1 cut(s) 554
BstF5I GGATG 3 cut(s) 187, 688, 822
BstFNI CGCG 2 cut(s) 216, 433
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 5 cut(s) 246, 673, 703, 784, 820
BstMBI GATC 5 cut(s) 243, 670, 700, 781, 817
BstMWI GCNNNNNNNGC 3 cut(s) 174, 245, 769
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 2 cut(s) 367, 735
BstSCI CCNGG 2 cut(s) 495, 772
BstSFI CTRYAG 2 cut(s) 276, 767
BstSLI GKGCMC 1 cut(s) 468
BstUI CGCG 2 cut(s) 216, 433
BstV1I GCAGC 4 cut(s) 39, 74, 375, 781
Bsu15I ATCGAT 1 cut(s) 813
BsuRI GGCC 2 cut(s) 168, 561
BsuTUI ATCGAT 1 cut(s) 813
BtgI CCRYGG 1 cut(s) 554
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 3 cut(s) 187, 688, 822
BtuMI TCGCGA 1 cut(s) 433
BveI ACCTGC 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 1 cut(s) 559
ClaI ATCGAT 1 cut(s) 813
Csp6I GTAC 4 cut(s) 49, 61, 367, 657
CviAII CATG 6 cut(s) 94, 364, 555, 698, 732, 833
CviQI GTAC 4 cut(s) 49, 61, 367, 657
DpnI GATC 5 cut(s) 245, 672, 702, 783, 819
DpnII GATC 5 cut(s) 243, 670, 700, 781, 817
Eam1104I CTCTTC 2 cut(s) 336, 720
EarI CTCTTC 2 cut(s) 336, 720
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 554
Eco24I GRGCYC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 495
EcoT14I CCWWGG 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 554
FaeI CATG 6 cut(s) 97, 367, 558, 701, 735, 836
FatI CATG 6 cut(s) 93, 363, 554, 697, 731, 832
FbaI TGATCA 4 cut(s) 670, 700, 781, 817
Fnu4HI GCNGC 4 cut(s) 28, 88, 389, 770
FokI GGATG 3 cut(s) 194, 695, 809
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 4 cut(s) 28, 88, 389, 770
FspBI CTAG 1 cut(s) 584
GlaI GCGC 1 cut(s) 362
GluI GCNGC 4 cut(s) 28, 88, 389, 770
GsaI CCCAGC 2 cut(s) 301, 321
HaeIII GGCC 2 cut(s) 168, 561
HapII CCGG 1 cut(s) 774
HhaI GCGC 1 cut(s) 363
Hin1II CATG 6 cut(s) 97, 367, 558, 701, 735, 836
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 2 cut(s) 141, 538
HpaII CCGG 1 cut(s) 774
HphI GGTGA 1 cut(s) 821
Hpy188I TCNGA 4 cut(s) 44, 243, 402, 738
Hpy188III TCNNGA 2 cut(s) 204, 432
HpyAV CCTTC 7 cut(s) 109, 129, 135, 141, 275, 767, 817
HpyCH4III ACNGT 3 cut(s) 470, 551, 855
HpyCH4V TGCA 4 cut(s) 177, 527, 697, 769
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 245, 769
Hsp92II CATG 6 cut(s) 97, 367, 558, 701, 735, 836
HspAI GCGC 1 cut(s) 361
KpnI GGTACC 1 cut(s) 660
Ksp22I TGATCA 4 cut(s) 670, 700, 781, 817
Kzo9I GATC 5 cut(s) 243, 670, 700, 781, 817
LmnI GCTCC 4 cut(s) 35, 95, 206, 385
Lsp1109I GCAGC 4 cut(s) 39, 74, 375, 781
LweI GCATC 3 cut(s) 446, 607, 699
MaeI CTAG 1 cut(s) 584
MaeIII GTNAC 1 cut(s) 827
MalI GATC 5 cut(s) 245, 672, 702, 783, 819
MboI GATC 5 cut(s) 243, 670, 700, 781, 817
MboII GAAGA 4 cut(s) 353, 364, 620, 737
MhlI GDGCHC 2 cut(s) 211, 468
MluCI AATT 6 cut(s) 150, 187, 577, 630, 666, 689
MluI ACGCGT 1 cut(s) 214
MnlI CCTC 9 cut(s) 38, 51, 126, 223, 232, 260, 280, 673, 721
MroXI GAANNNNTTC 1 cut(s) 569
MseI TTAA 5 cut(s) 546, 633, 642, 665, 846
MspI CCGG 1 cut(s) 774
MspR9I CCNGG 2 cut(s) 497, 774
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 1 cut(s) 497
MvnI CGCG 2 cut(s) 216, 433
MwoI GCNNNNNNNGC 3 cut(s) 174, 245, 769
NciI CCSGG 1 cut(s) 774
NcoI CCATGG 1 cut(s) 554
NdeII GATC 5 cut(s) 243, 670, 700, 781, 817
NlaIII CATG 6 cut(s) 97, 367, 558, 701, 735, 836
NlaIV GGNNCC 2 cut(s) 560, 658
NmeAIII GCCGAG 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 827
NruI TCGCGA 1 cut(s) 433
NspI RCATGY 2 cut(s) 367, 735
PctI GAATGC 1 cut(s) 745
PdmI GAANNNNTTC 1 cut(s) 569
PfeI GAWTC 2 cut(s) 141, 538
PkrI GCNGC 4 cut(s) 29, 89, 390, 771
PsiI TTATAA 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 495
PspFI CCCAGC 2 cut(s) 297, 317
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 2 cut(s) 560, 658
PspPI GGNCC 1 cut(s) 559
PstI CTGCAG 1 cut(s) 771
PstNI CAGNNNCTG 1 cut(s) 518
RruI TCGCGA 1 cut(s) 433
RsaI GTAC 4 cut(s) 50, 62, 368, 658
RsaNI GTAC 4 cut(s) 49, 61, 367, 657
SacI GAGCTC 1 cut(s) 211
SaqAI TTAA 5 cut(s) 546, 633, 642, 665, 846
SatI GCNGC 4 cut(s) 28, 88, 389, 770
Sau3AI GATC 5 cut(s) 243, 670, 700, 781, 817
Sau96I GGNCC 1 cut(s) 559
ScrFI CCNGG 2 cut(s) 497, 774
SduI GDGCHC 2 cut(s) 211, 468
SfaNI GCATC 3 cut(s) 446, 607, 699
SfcI CTRYAG 2 cut(s) 276, 767
SmlI CTYRAG 2 cut(s) 223, 837
SmoI CTYRAG 2 cut(s) 223, 837
Sse9I AATT 6 cut(s) 150, 187, 577, 630, 666, 689
SsiI CCGC 1 cut(s) 290
SspMI CTAG 1 cut(s) 584
SstI GAGCTC 1 cut(s) 211
StyD4I CCNGG 2 cut(s) 495, 772
StyI CCWWGG 1 cut(s) 554
TaaI ACNGT 3 cut(s) 470, 551, 855
TaqI TCGA 1 cut(s) 813
TasI AATT 6 cut(s) 150, 187, 577, 630, 666, 689
TfiI GAWTC 2 cut(s) 141, 538
Tru1I TTAA 5 cut(s) 546, 633, 642, 665, 846
Tru9I TTAA 5 cut(s) 546, 633, 642, 665, 846
TseFI GTSAC 1 cut(s) 827
TseI GCWGC 4 cut(s) 27, 87, 388, 769
Tsp45I GTSAC 1 cut(s) 827
TspDTI ATGAA 3 cut(s) 198, 787, 799
TspGWI ACGGA 2 cut(s) 194, 815
XapI RAATTY 2 cut(s) 187, 689
XceI RCATGY 2 cut(s) 367, 735
XmnI GAANNNNTTC 1 cut(s) 569
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.