Rh3AG256000

Serine hydrolase involved in the detoxification of formaldehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0012329
Physical Location & Seq
Forward (+)
27979791 .. 27985754
5964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG256000.1

Sequence Viewer

Length: 870 bp
ATGGAGGAGTGTAAGCCCACTGAGATCAGCAGCTCAAAGATGTTTGGAGGTTACAACAAGAGATACAAGCATTTCAGTCCTACTCTGGGCTGCTCCATGACCTTCCACATCTATTTTCCTCCTTCTCCTTCCCCTTCCCACAAATTCCCCGTTCTTTACTGGCTCTCTGGCCTTACCTGCACAGATGAGAATTTCATAACCAAATCAGGAGCTCAGCGTTCTGCGGCAAGGGAGGGTATTGCTTTGATTGCCCCTGATACATCTCCTAGAGGCTTGTCTATTGAAGGAGAAGCAGATAGCTGGGATTTTGGTGTAGGTGCTGGATTTTATCTCAATGCTACCCAGGAGAAATGGAAGAACTGGCGTATGTATGATTATGTTGTCAAGGAGTTGCCCAAACTTCTGAATGAAAATTTTCCACAGCTTGATACATTGCGGGCATCTATATCTGGTCACTCAATGGGTGGACATGGTGCTTTGACAATCTACCTGAAAAACCTAGACAAATATAAGTCAGTATCTGCCTTCGCACCAATTGTGAATCCTATCAACTGCCCATGGGGTCAGAAAGCTTTCTCAAATTATCTAGGTGGCAATAAATCTGACTGGGAGGAATATGATGCCACTTGCCTAGTTAAGAAGTTGAATGATGTGTCAGCCACCATTTTAATTGATCAGGGGGAGGATGACAAATTCTTGCGTGATCAGCTACTACCACATAAGTTTGCAGAGGCATGCAGCAGTGCTAAAGTTCCACTTCTACTGCGTACCCAGCCCGGTTATGATCACTCATACTATTTCATTGCTACCTTCATAGACGATCACATCAGTCACCATGCTCAAGCTGTTAAACTGCCTACACCACCATGA

Protein Analysis

289

Amino Acids

32.36

Weight (kDa)

6.55

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Esterase PF00756 26 - 276 5.7e-61 Putative esterase
Peptidase_S9 PF00326 110 - 266 7.5e-09 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41530
fragaria_vesca FvH4_6g24520
malus_domestica MD12G1082500.v1.1 MD14G1077300.v1.1
prunus_persica Prupe.7G053400_v2.0.a1 Prupe.7G053400_v2.0.a1
pyrus_communis pycom12g06870 pycom14g06610
rosa_chinensis RchiOBHm_Chr3g0483631
rosa_laevigata RLG00000023263
rosa_multiflora Rmu_co8515891.1_g000001 Rmu_sc0000555.1_g000009
rosa_roxburghii Rroxscaffold_6G00398170
rosa_rugosa Rorug03G0207500
rosa_samantha Rh3AG256000 Rh3BG292700 Rh3CG290400 Rh3DG286300
rosa_wichuraiana Rw3G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
AciI CCGC 2 cut(s) 224, 436
AcsI RAATTY 4 cut(s) 143, 190, 412, 692
AfaI GTAC 1 cut(s) 769
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 2 cut(s) 284, 646
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 7 cut(s) 33, 212, 300, 424, 572, 709, 845
AluI AGCT 7 cut(s) 33, 212, 300, 424, 572, 709, 845
Alw21I GWGCWC 1 cut(s) 214
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 3 cut(s) 30, 90, 738
ApoI RAATTY 4 cut(s) 143, 190, 412, 692
Asp700I GAANNNNTTC 2 cut(s) 414, 572
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 1 cut(s) 824
BanII GRGCYC 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 3 cut(s) 42, 77, 750
BciT130I CCWGG 1 cut(s) 344
BclI TGATCA 3 cut(s) 673, 703, 784
BcnI CCSGG 1 cut(s) 777
BfaI CTAG 4 cut(s) 267, 500, 587, 632
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 4 cut(s) 31, 91, 225, 739
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 4 cut(s) 32, 92, 226, 740
Bme1390I CCNGG 2 cut(s) 344, 777
BmrFI CCNGG 2 cut(s) 344, 777
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 2 cut(s) 449, 610
BmuI ACTGGG 1 cut(s) 616
Bpu1102I GCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 825
BpuMI CCSGG 1 cut(s) 777
BsaJI CCNNGG 2 cut(s) 342, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 3 cut(s) 164, 365, 611
Bse3DI GCAATG 2 cut(s) 431, 801
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 2 cut(s) 342, 557
BseGI GGATG 1 cut(s) 691
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 2 cut(s) 431, 801
BseMII CTCAG 2 cut(s) 12, 227
BseNI ACTGG 3 cut(s) 164, 365, 611
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 42, 77, 750
BseYI CCCAGC 2 cut(s) 300, 771
BsgI GTGCAG 1 cut(s) 163
BshFI GGCC 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 1 cut(s) 777
BslI CCNNNNNNNGG 1 cut(s) 86
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 5 cut(s) 24, 673, 703, 784, 820
Bsp1720I GCTNAGC 1 cut(s) 213
Bsp19I CCATGG 1 cut(s) 557
BspACI CCGC 2 cut(s) 224, 436
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 2 cut(s) 13, 226
BspMI ACCTGC 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 431, 801
BsrI ACTGG 3 cut(s) 164, 365, 611
BssECI CCNNGG 2 cut(s) 342, 557
BssMI GATC 5 cut(s) 24, 673, 703, 784, 820
BssT1I CCWWGG 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 438, 736
BstDEI CTNAG 2 cut(s) 21, 213
BstDSI CCRYGG 1 cut(s) 557
BstF5I GGATG 1 cut(s) 691
BstKTI GATC 5 cut(s) 27, 676, 706, 787, 823
BstMBI GATC 5 cut(s) 24, 673, 703, 784, 820
BstMWI GCNNNNNNNGC 4 cut(s) 177, 248, 706, 772
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 1 cut(s) 738
BstSCI CCNGG 2 cut(s) 342, 775
BstV1I GCAGC 3 cut(s) 42, 77, 750
BsuRI GGCC 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 557
BtsCI GGATG 1 cut(s) 691
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 2 cut(s) 18, 748
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 2 cut(s) 438, 736
CaiI CAGNNNCTG 1 cut(s) 521
Csp6I GTAC 1 cut(s) 768
CviAII CATG 6 cut(s) 97, 470, 558, 735, 836, 867
CviQI GTAC 1 cut(s) 768
DdeI CTNAG 2 cut(s) 21, 213
DpnI GATC 5 cut(s) 26, 675, 705, 786, 822
DpnII GATC 5 cut(s) 24, 673, 703, 784, 820
Ecl136II GAGCTC 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 557
Eco24I GRGCYC 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 557
EcoT38I GRGCYC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 6 cut(s) 100, 473, 561, 738, 839, 870
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FatI CATG 6 cut(s) 96, 469, 557, 734, 835, 866
FauI CCCGC 1 cut(s) 429
FbaI TGATCA 3 cut(s) 673, 703, 784
Fnu4HI GCNGC 4 cut(s) 31, 91, 225, 739
FokI GGATG 1 cut(s) 698
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 4 cut(s) 31, 91, 225, 739
FspBI CTAG 4 cut(s) 267, 500, 587, 632
GluI GCNGC 4 cut(s) 31, 91, 225, 739
GsaI CCCAGC 2 cut(s) 304, 775
HaeIII GGCC 1 cut(s) 171
HapII CCGG 1 cut(s) 777
Hin1II CATG 6 cut(s) 100, 473, 561, 738, 839, 870
HindIII AAGCTT 1 cut(s) 570
HinfI GANTC 1 cut(s) 541
HpaII CCGG 1 cut(s) 777
HphI GGTGA 1 cut(s) 824
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 3 cut(s) 405, 567, 604
Hpy188III TCNNGA 1 cut(s) 207
Hpy8I GTNNAC 1 cut(s) 467
HpyAV CCTTC 7 cut(s) 112, 132, 138, 144, 278, 535, 820
HpyCH4V TGCA 3 cut(s) 180, 728, 738
HpyF10VI GCNNNNNNNGC 4 cut(s) 177, 248, 706, 772
HpyF3I CTNAG 2 cut(s) 21, 213
Hsp92II CATG 6 cut(s) 100, 473, 561, 738, 839, 870
Ksp22I TGATCA 3 cut(s) 673, 703, 784
Kzo9I GATC 5 cut(s) 24, 673, 703, 784, 820
LmnI GCTCC 2 cut(s) 98, 209
Lsp1109I GCAGC 3 cut(s) 42, 77, 750
LweI GCATC 2 cut(s) 449, 610
MaeI CTAG 4 cut(s) 267, 500, 587, 632
MaeIII GTNAC 3 cut(s) 50, 452, 830
MalI GATC 5 cut(s) 26, 675, 705, 786, 822
MboI GATC 5 cut(s) 24, 673, 703, 784, 820
MboII GAAGA 1 cut(s) 367
MfeI CAATTG 1 cut(s) 534
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 7 cut(s) 143, 190, 412, 534, 580, 669, 692
MnlI CCTC 7 cut(s) 41, 129, 226, 263, 604, 676, 724
MroXI GAANNNNTTC 2 cut(s) 414, 572
MseI TTAA 3 cut(s) 636, 668, 849
MslI CAYNNNNRTG 1 cut(s) 865
MspI CCGG 1 cut(s) 777
MspR9I CCNGG 2 cut(s) 344, 777
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 344
MwoI GCNNNNNNNGC 4 cut(s) 177, 248, 706, 772
NciI CCSGG 1 cut(s) 777
NcoI CCATGG 1 cut(s) 557
NdeII GATC 5 cut(s) 24, 673, 703, 784, 820
NlaIII CATG 6 cut(s) 100, 473, 561, 738, 839, 870
NmuCI GTSAC 2 cut(s) 452, 830
NspI RCATGY 1 cut(s) 738
PaeI GCATGC 1 cut(s) 738
PdmI GAANNNNTTC 2 cut(s) 414, 572
PfeI GAWTC 1 cut(s) 541
PkrI GCNGC 4 cut(s) 32, 92, 226, 740
Psp124BI GAGCTC 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 342
PspFI CCCAGC 2 cut(s) 300, 771
PspGI CCWGG 1 cut(s) 342
PstNI CAGNNNCTG 1 cut(s) 521
RsaI GTAC 1 cut(s) 769
RsaNI GTAC 1 cut(s) 768
RseI CAYNNNNRTG 1 cut(s) 865
SacI GAGCTC 1 cut(s) 214
SaqAI TTAA 3 cut(s) 636, 668, 849
SatI GCNGC 4 cut(s) 31, 91, 225, 739
Sau3AI GATC 5 cut(s) 24, 673, 703, 784, 820
ScrFI CCNGG 2 cut(s) 344, 777
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 2 cut(s) 449, 610
SmiMI CAYNNNNRTG 1 cut(s) 865
SmlI CTYRAG 1 cut(s) 840
SmoI CTYRAG 1 cut(s) 840
SphI GCATGC 1 cut(s) 738
Sse9I AATT 7 cut(s) 143, 190, 412, 534, 580, 669, 692
SsiI CCGC 2 cut(s) 224, 436
SspMI CTAG 4 cut(s) 267, 500, 587, 632
SstI GAGCTC 1 cut(s) 214
StyD4I CCNGG 2 cut(s) 342, 775
StyI CCWWGG 1 cut(s) 557
TasI AATT 7 cut(s) 143, 190, 412, 534, 580, 669, 692
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 3 cut(s) 636, 668, 849
Tru9I TTAA 3 cut(s) 636, 668, 849
TscAI CASTG 2 cut(s) 25, 748
TseFI GTSAC 2 cut(s) 452, 830
TseI GCWGC 3 cut(s) 30, 90, 738
Tsp45I GTSAC 2 cut(s) 452, 830
TspDTI ATGAA 4 cut(s) 184, 423, 790, 802
TspRI CASTG 2 cut(s) 25, 748
XapI RAATTY 4 cut(s) 143, 190, 412, 692
XceI RCATGY 1 cut(s) 738
XmnI GAANNNNTTC 2 cut(s) 414, 572
XspI CTAG 4 cut(s) 267, 500, 587, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.