FvH4_6g26190

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007999
Physical Location & Seq
Forward (+)
20039270 .. 20042710
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26190.t3

Sequence Viewer

Length: 1143 bp
ATGGCCTCCCCCTCTAGGGCCATCGCATTCAAATGCTCTTCCTCTCTCGCCTTTTCTTCAAAAACGCGGATGGACTCCAAACCCAGAACCCTCCATAACTCATTTTCAAGTTCAAGCGCTGCTCCAAGAGAAGCTACTGAAGCCTCTATCACATTGCAGGAATGGCAGGGCTGGGGCACCACCTCCCCTGTGCCCGCTTTGGTCACCAAAATTGTTCAGGATTTGAAGGTTTTGGAGAAGGCTACCGATGCACGAATGAGCTTTGGCGGCAGTGGTGGCAAGCTTCAGGGAGATTTTAAAGTGCAGGAGGATAGAAAACACAGGAAGACATATAAGGCTTTAGGTGATTCTGAACAAAAGCTTCAGTTTTTCTCAGCTCGACAAATAGCATGCCGCGTGCTTGGGAGTAGAGGCTACCTTTGTCAAAAGTGCTGGCTACCTTTGGAAGATTGTATGTGTGCAAGAATAGTGAAGTACCCTCTTTGGCCTCGAATGCGATTCTGGTTATATATGCATCCAAAGGATTTCTTAAGGCAGAACAATACTGGAAAGTTGTTGTGGCAAGTATTTGGCACTGAAGCTGCAACTTTGTGCCTCTTCGGCATCGTGGAACAGGAAGAAATAATGTGGGATGCATTTAAGCTTGCAGGAAAAAGCAATGTGTGGTGCCTCTATCCCAACAAAAATGCTGTGGTAACATCTGTTCAGGATGTATTTGGCCAAGAATCTTCAGCTGATCTGGAATGTCCATATACGAAGATAAATGAAGACAGAACTCTAAATTTCATTTTGATCGATGGAACATGGAACAATTCTGTTGCAATATTTCGTCGTCTGAAGGATCAAGCAAAGTTAATATGGGGAGGAGAGGACTTCCCTTGTATTTCCCTTGCTACTGGTGCTTCTGCAATGCATAAACTTAGGCCCCAACCATCATTGGATCGTACCTGTACAGCAGCAGCAGCTATTGGCCTCCTCTCCGAGCTGCATCTTCTTCCAGAGTTTAGCTCCCATGGATTTGATAAGAAGGCTGAAGCTGTGGAGGATGCTTTAGTTGTATTATTAGAGGCTTTAACAACTAGACGACTTCGGATGGGCAGGTCCATCAGTCGTACAGTGAGACATAACAGCGATTTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

381

Amino Acids

42.64

Weight (kDa)

9.09

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 139 - 281 3e-28 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1089
AccB1I GGYRCC 2 cut(s) 176, 666
AccII CGCG 2 cut(s) 67, 396
AciI CCGC 4 cut(s) 67, 195, 267, 394
AclWI GGATC 2 cut(s) 849, 948
AcoI YGGCCR 1 cut(s) 718
AcsI RAATTY 1 cut(s) 781
AcuI CTGAAG 7 cut(s) 159, 269, 347, 597, 714, 857, 1053
AfaI GTAC 4 cut(s) 476, 946, 952, 1114
AfeI AGCGCT 1 cut(s) 118
AfiI CCNNNNNNNGG 2 cut(s) 15, 16
AflII CTTAAG 1 cut(s) 529
AgsI TTSAA 5 cut(s) 31, 60, 108, 114, 226
Alw26I GTCTC 1 cut(s) 1114
AlwI GGATC 2 cut(s) 849, 948
Aor51HI AGCGCT 1 cut(s) 118
AoxI GGCC 6 cut(s) 3, 18, 485, 718, 923, 970
ApeKI GCWGC 6 cut(s) 119, 581, 956, 959, 962, 985
ApoI RAATTY 1 cut(s) 781
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 3 cut(s) 18, 924, 1101
AsuHPI GGTGA 2 cut(s) 196, 356
AvaII GGWCC 1 cut(s) 1101
BaeGI GKGCMC 2 cut(s) 179, 195
BalI TGGCCA 1 cut(s) 720
BanI GGYRCC 2 cut(s) 176, 666
BbsI GAAGAC 2 cut(s) 332, 774
BbvI GCAGC 6 cut(s) 106, 568, 968, 971, 972, 974
BccI CCATC 6 cut(s) 29, 64, 791, 940, 1087, 1112
BcoDI GTCTC 1 cut(s) 1114
BfaI CTAG 2 cut(s) 15, 1080
BfoI RGCGCY 1 cut(s) 120
BfrI CTTAAG 1 cut(s) 529
BfuAI ACCTGC 1 cut(s) 1089
BglI GCCNNNNNGGC 1 cut(s) 600
BisI GCNGC 8 cut(s) 120, 268, 394, 582, 957, 960, 963, 986
BlsI GCNGC 8 cut(s) 121, 269, 395, 583, 958, 961, 964, 987
Bme18I GGWCC 1 cut(s) 1101
BmgT120I GGNCC 3 cut(s) 18, 924, 1101
BmiI GGNNCC 3 cut(s) 178, 668, 926
BmsI GCATC 6 cut(s) 238, 523, 612, 622, 997, 1036
BpiI GAAGAC 2 cut(s) 332, 774
BplI GAGNNNNNCTC 2 cut(s) 992, 1024
Bsa29I ATCGAT 1 cut(s) 795
BsaJI CCNNGG 1 cut(s) 1012
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 16
Bse1I ACTGG 2 cut(s) 550, 901
Bse3DI GCAATG 3 cut(s) 152, 664, 915
BseCI ATCGAT 1 cut(s) 795
BseDI CCNNGG 1 cut(s) 1012
BseGI GGATG 6 cut(s) 75, 514, 637, 715, 1051, 1098
BseLI CCNNNNNNNGG 2 cut(s) 15, 16
BseMI GCAATG 3 cut(s) 152, 664, 915
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 550, 901
BseRI GAGGAG 2 cut(s) 879, 965
BseSI GKGCMC 2 cut(s) 179, 195
BseXI GCAGC 6 cut(s) 106, 568, 968, 971, 972, 974
BseYI CCCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 323
Bsh1236I CGCG 2 cut(s) 67, 396
BshFI GGCC 6 cut(s) 5, 20, 487, 720, 925, 972
BshNI GGYRCC 2 cut(s) 176, 666
BshVI ATCGAT 1 cut(s) 795
BslI CCNNNNNNNGG 2 cut(s) 15, 16
BsmAI GTCTC 1 cut(s) 1114
BsmI GAATGC 2 cut(s) 26, 498
BsnI GGCC 6 cut(s) 5, 20, 487, 720, 925, 972
Bsp1286I GDGCHC 2 cut(s) 179, 195
Bsp1407I TGTACA 1 cut(s) 950
Bsp143I GATC 4 cut(s) 736, 792, 841, 940
Bsp19I CCATGG 1 cut(s) 1012
BspACI CCGC 4 cut(s) 67, 195, 267, 394
BspANI GGCC 6 cut(s) 5, 20, 487, 720, 925, 972
BspCNI CTCAG 1 cut(s) 386
BspDI ATCGAT 1 cut(s) 795
BspFNI CGCG 2 cut(s) 67, 396
BspLI GGNNCC 3 cut(s) 178, 668, 926
BspMI ACCTGC 1 cut(s) 1089
BspPI GGATC 2 cut(s) 849, 948
BspQI GCTCTTC 1 cut(s) 43
BspT107I GGYRCC 2 cut(s) 176, 666
BspTI CTTAAG 1 cut(s) 529
BsrDI GCAATG 3 cut(s) 152, 664, 915
BsrGI TGTACA 1 cut(s) 950
BsrI ACTGG 2 cut(s) 550, 901
BssECI CCNNGG 1 cut(s) 1012
BssMI GATC 4 cut(s) 736, 792, 841, 940
BssT1I CCWWGG 1 cut(s) 1012
Bst4CI ACNGT 1 cut(s) 1117
Bst6I CTCTTC 2 cut(s) 43, 602
BstAFI CTTAAG 1 cut(s) 529
BstAUI TGTACA 1 cut(s) 950
BstC8I GCNNGC 6 cut(s) 195, 281, 391, 398, 434, 645
BstDEI CTNAG 2 cut(s) 373, 920
BstDSI CCRYGG 1 cut(s) 1012
BstEII GGTNACC 1 cut(s) 202
BstF5I GGATG 6 cut(s) 75, 514, 637, 715, 1051, 1098
BstFNI CGCG 2 cut(s) 67, 396
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 4 cut(s) 739, 795, 844, 943
BstMAI GTCTC 1 cut(s) 1114
BstMBI GATC 4 cut(s) 736, 792, 841, 940
BstMWI GCNNNNNNNGC 9 cut(s) 140, 163, 248, 267, 276, 493, 600, 899, 962
BstNSI RCATGY 1 cut(s) 393
BstPI GGTNACC 1 cut(s) 202
BstSLI GKGCMC 2 cut(s) 179, 195
BstUI CGCG 2 cut(s) 67, 396
BstV1I GCAGC 6 cut(s) 106, 568, 968, 971, 972, 974
BstV2I GAAGAC 2 cut(s) 332, 774
Bsu15I ATCGAT 1 cut(s) 795
BsuRI GGCC 6 cut(s) 5, 20, 487, 720, 925, 972
BsuTUI ATCGAT 1 cut(s) 795
BtgI CCRYGG 1 cut(s) 1012
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 6 cut(s) 75, 514, 637, 715, 1051, 1098
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 3 cut(s) 277, 573, 1122
BveI ACCTGC 1 cut(s) 1089
Cac8I GCNNGC 6 cut(s) 195, 281, 391, 398, 434, 645
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 18, 924, 1101
ClaI ATCGAT 1 cut(s) 795
Csp6I GTAC 4 cut(s) 475, 945, 951, 1113
CviAII CATG 3 cut(s) 390, 804, 1013
CviQI GTAC 4 cut(s) 475, 945, 951, 1113
DdeI CTNAG 2 cut(s) 373, 920
DpnI GATC 4 cut(s) 738, 794, 843, 942
DpnII GATC 4 cut(s) 736, 792, 841, 940
DraI TTTAAA 1 cut(s) 298
EaeI YGGCCR 1 cut(s) 718
Eam1104I CTCTTC 2 cut(s) 43, 602
EarI CTCTTC 2 cut(s) 43, 602
Eco130I CCWWGG 1 cut(s) 1012
Eco47I GGWCC 1 cut(s) 1101
Eco47III AGCGCT 1 cut(s) 118
Eco57I CTGAAG 7 cut(s) 159, 269, 347, 597, 714, 857, 1053
Eco91I GGTNACC 1 cut(s) 202
EcoO109I RGGNCCY 1 cut(s) 924
EcoO65I GGTNACC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 1012
EcoT22I ATGCAT 3 cut(s) 516, 637, 915
ErhI CCWWGG 1 cut(s) 1012
FaeI CATG 3 cut(s) 393, 807, 1016
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FatI CATG 3 cut(s) 389, 803, 1012
FauI CCCGC 1 cut(s) 202
Fnu4HI GCNGC 8 cut(s) 120, 268, 394, 582, 957, 960, 963, 986
FokI GGATG 6 cut(s) 82, 501, 644, 722, 1058, 1105
Fsp4HI GCNGC 8 cut(s) 120, 268, 394, 582, 957, 960, 963, 986
FspBI CTAG 2 cut(s) 15, 1080
GlaI GCGC 1 cut(s) 118
GluI GCNGC 8 cut(s) 120, 268, 394, 582, 957, 960, 963, 986
GsaI CCCAGC 1 cut(s) 175
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 6 cut(s) 5, 20, 487, 720, 925, 972
HhaI GCGC 1 cut(s) 119
Hin1II CATG 3 cut(s) 393, 807, 1016
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 3 cut(s) 281, 359, 641
HinfI GANTC 4 cut(s) 74, 347, 498, 725
HphI GGTGA 2 cut(s) 196, 356
Hpy188I TCNGA 4 cut(s) 352, 837, 982, 1092
Hpy188III TCNNGA 4 cut(s) 218, 707, 740, 998
Hpy99I CGWCG 1 cut(s) 834
HpyAV CCTTC 4 cut(s) 220, 232, 832, 1021
HpyCH4III ACNGT 1 cut(s) 1117
HpyF10VI GCNNNNNNNGC 9 cut(s) 140, 163, 248, 267, 276, 493, 600, 899, 962
HpyF3I CTNAG 2 cut(s) 373, 920
Hsp92II CATG 3 cut(s) 393, 807, 1016
HspAI GCGC 1 cut(s) 117
Kzo9I GATC 4 cut(s) 736, 792, 841, 940
LguI GCTCTTC 1 cut(s) 43
LmnI GCTCC 2 cut(s) 127, 1013
Lsp1109I GCAGC 6 cut(s) 106, 568, 968, 971, 972, 974
LweI GCATC 6 cut(s) 238, 523, 612, 622, 997, 1036
MaeI CTAG 2 cut(s) 15, 1080
MaeIII GTNAC 2 cut(s) 202, 694
MalI GATC 4 cut(s) 738, 794, 843, 942
MboI GATC 4 cut(s) 736, 792, 841, 940
MhlI GDGCHC 2 cut(s) 179, 195
MlsI TGGCCA 1 cut(s) 720
MluCI AATT 3 cut(s) 210, 781, 811
MluNI TGGCCA 1 cut(s) 720
MlyI GAGTC 1 cut(s) 68
Mox20I TGGCCA 1 cut(s) 720
Mph1103I ATGCAT 3 cut(s) 516, 637, 915
MscI TGGCCA 1 cut(s) 720
MseI TTAA 6 cut(s) 297, 530, 639, 854, 1073, 1141
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 1 cut(s) 720
MspA1I CMGCKG 1 cut(s) 734
MspCI CTTAAG 1 cut(s) 529
Mva1269I GAATGC 2 cut(s) 26, 498
MvnI CGCG 2 cut(s) 67, 396
MwoI GCNNNNNNNGC 9 cut(s) 140, 163, 248, 267, 276, 493, 600, 899, 962
NcoI CCATGG 1 cut(s) 1012
NdeII GATC 4 cut(s) 736, 792, 841, 940
NlaIII CATG 3 cut(s) 393, 807, 1016
NlaIV GGNNCC 3 cut(s) 178, 668, 926
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 3 cut(s) 516, 637, 915
NspI RCATGY 1 cut(s) 393
PaeI GCATGC 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 43
PctI GAATGC 2 cut(s) 26, 498
PfeI GAWTC 3 cut(s) 347, 498, 725
PkrI GCNGC 8 cut(s) 121, 269, 395, 583, 958, 961, 964, 987
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PspEI GGTNACC 1 cut(s) 202
PspFI CCCAGC 1 cut(s) 171
PspN4I GGNNCC 3 cut(s) 178, 668, 926
PspPI GGNCC 3 cut(s) 18, 924, 1101
PsrI GAACNNNNNNTAC 2 cut(s) 687, 719
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 4 cut(s) 476, 946, 952, 1114
RsaNI GTAC 4 cut(s) 475, 945, 951, 1113
RseI CAYNNNNRTG 1 cut(s) 31
SapI GCTCTTC 1 cut(s) 43
SaqAI TTAA 6 cut(s) 297, 530, 639, 854, 1073, 1141
SatI GCNGC 8 cut(s) 120, 268, 394, 582, 957, 960, 963, 986
Sau3AI GATC 4 cut(s) 736, 792, 841, 940
Sau96I GGNCC 3 cut(s) 18, 924, 1101
SchI GAGTC 1 cut(s) 68
SduI GDGCHC 2 cut(s) 179, 195
SfaNI GCATC 6 cut(s) 238, 523, 612, 622, 997, 1036
SinI GGWCC 1 cut(s) 1101
SmiMI CAYNNNNRTG 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
SphI GCATGC 1 cut(s) 393
Sse9I AATT 3 cut(s) 210, 781, 811
SsiI CCGC 4 cut(s) 67, 195, 267, 394
SspI AATATT 1 cut(s) 825
SspMI CTAG 2 cut(s) 15, 1080
StyI CCWWGG 1 cut(s) 1012
TaaI ACNGT 1 cut(s) 1117
TaqI TCGA 3 cut(s) 379, 490, 795
TasI AATT 3 cut(s) 210, 781, 811
TatI WGTACW 1 cut(s) 950
TauI GCSGC 2 cut(s) 270, 396
TfiI GAWTC 3 cut(s) 347, 498, 725
Tru1I TTAA 6 cut(s) 297, 530, 639, 854, 1073, 1141
Tru9I TTAA 6 cut(s) 297, 530, 639, 854, 1073, 1141
TscAI CASTG 3 cut(s) 277, 580, 1122
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 6 cut(s) 119, 581, 956, 959, 962, 985
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 775, 780
TspRI CASTG 3 cut(s) 277, 580, 1122
Vha464I CTTAAG 1 cut(s) 529
VpaK11BI GGWCC 1 cut(s) 1101
XapI RAATTY 1 cut(s) 781
XceI RCATGY 1 cut(s) 393
XspI CTAG 2 cut(s) 15, 1080
Zsp2I ATGCAT 3 cut(s) 516, 637, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.