Rmu_ssc0000476.1_g000001

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000476.1
OG0007999
Physical Location & Seq
Reverse (-)
3265 .. 3649
385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000476.1_g000001.1.cds

Sequence Viewer

Length: 234 bp
atgcataaacttcggccccaaccatcattggatcgtacctgtacagcagcagcagctgttggcctcctctccgagctgcacgttcttccagagttcaggtcccatggatttgataagcaggctgaagttgtagaggatgctttagttgtactattagaggctctaacaaatagacgacttcggatgggcaggtccatcagtcgtaaagtaagacatacgagcgattttggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

77

Amino Acids

8.64

Weight (kDa)

9.76

Isoelectric Point (pI)

67.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 180
AclWI GGATC 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 144
AfaI GTAC 3 cut(s) 37, 43, 150
AluBI AGCT 2 cut(s) 56, 76
AluI AGCT 2 cut(s) 56, 76
AlwI GGATC 1 cut(s) 39
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 2 cut(s) 14, 61
ApeKI GCWGC 4 cut(s) 47, 50, 53, 76
AspS9I GGNCC 3 cut(s) 15, 99, 192
AvaII GGWCC 2 cut(s) 99, 192
BbvI GCAGC 4 cut(s) 59, 62, 63, 65
BccI CCATC 3 cut(s) 31, 178, 203
BcgI CGANNNNNNTGC 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 180
BisI GCNGC 4 cut(s) 48, 51, 54, 77
BlsI GCNGC 4 cut(s) 49, 52, 55, 78
Bme18I GGWCC 2 cut(s) 99, 192
BmgT120I GGNCC 3 cut(s) 15, 99, 192
BmiI GGNNCC 2 cut(s) 17, 101
BmsI GCATC 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 2 cut(s) 142, 189
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 4 cut(s) 59, 62, 63, 65
BsgI GTGCAG 1 cut(s) 62
BshFI GGCC 2 cut(s) 16, 63
BslFI GGGAC 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 2 cut(s) 16, 63
Bsp1407I TGTACA 1 cut(s) 41
Bsp143I GATC 1 cut(s) 31
Bsp19I CCATGG 1 cut(s) 103
BspANI GGCC 2 cut(s) 16, 63
BspLI GGNNCC 2 cut(s) 17, 101
BspMI ACCTGC 1 cut(s) 180
BspPI GGATC 1 cut(s) 39
BsrGI TGTACA 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 1 cut(s) 31
BssT1I CCWWGG 1 cut(s) 103
BstAUI TGTACA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 120
BstDSI CCRYGG 1 cut(s) 103
BstF5I GGATG 2 cut(s) 142, 189
BstKTI GATC 1 cut(s) 34
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstV1I GCAGC 4 cut(s) 59, 62, 63, 65
BsuRI GGCC 2 cut(s) 16, 63
BtgI CCRYGG 1 cut(s) 103
BtsCI GGATG 2 cut(s) 142, 189
BveI ACCTGC 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 120
CaiI CAGNNNCTG 1 cut(s) 56
Cfr13I GGNCC 3 cut(s) 15, 99, 192
Csp6I GTAC 3 cut(s) 36, 42, 149
CviAII CATG 1 cut(s) 104
CviJI RGCY 6 cut(s) 16, 56, 63, 76, 122, 161
CviKI_1 RGCY 6 cut(s) 16, 56, 63, 76, 122, 161
CviQI GTAC 3 cut(s) 36, 42, 149
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 103
Eco47I GGWCC 2 cut(s) 99, 192
Eco57I CTGAAG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 99
EcoT14I CCWWGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 1 cut(s) 107
FaiI YATR 3 cut(s) 6, 105, 216
FaqI GGGAC 1 cut(s) 85
FatI CATG 1 cut(s) 103
Fnu4HI GCNGC 4 cut(s) 48, 51, 54, 77
FokI GGATG 2 cut(s) 149, 196
Fsp4HI GCNGC 4 cut(s) 48, 51, 54, 77
GluI GCNGC 4 cut(s) 48, 51, 54, 77
HaeIII GGCC 2 cut(s) 16, 63
Hin1II CATG 1 cut(s) 107
Hpy188I TCNGA 2 cut(s) 73, 183
Hpy188III TCNNGA 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 4, 79
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 1 cut(s) 107
Kzo9I GATC 1 cut(s) 31
LpnPI CCDG 5 cut(s) 52, 82, 102, 104, 175
Lsp1109I GCAGC 4 cut(s) 59, 62, 63, 65
LweI GCATC 1 cut(s) 127
MaeII ACGT 1 cut(s) 81
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 1 cut(s) 77
MnlI CCTC 4 cut(s) 74, 77, 127, 151
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 232
MspA1I CMGCKG 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 53
NcoI CCATGG 1 cut(s) 103
NdeII GATC 1 cut(s) 31
NlaIII CATG 1 cut(s) 107
NlaIV GGNNCC 2 cut(s) 17, 101
NsiI ATGCAT 1 cut(s) 6
PkrI GCNGC 4 cut(s) 49, 52, 55, 78
PpuMI RGGWCCY 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 99
PspN4I GGNNCC 2 cut(s) 17, 101
PspPI GGNCC 3 cut(s) 15, 99, 192
PspPPI RGGWCCY 1 cut(s) 99
PstNI CAGNNNCTG 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 3 cut(s) 37, 43, 150
RsaNI GTAC 3 cut(s) 36, 42, 149
SaqAI TTAA 1 cut(s) 232
SatI GCNGC 4 cut(s) 48, 51, 54, 77
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 3 cut(s) 15, 99, 192
SetI ASST 6 cut(s) 41, 58, 78, 84, 101, 194
SfaNI GCATC 1 cut(s) 127
SgeI CNNG 8 cut(s) 51, 85, 92, 101, 109, 116, 131, 202
SinI GGWCC 2 cut(s) 99, 192
StyI CCWWGG 1 cut(s) 103
TaiI ACGT 1 cut(s) 84
TatI WGTACW 2 cut(s) 41, 148
Tru1I TTAA 1 cut(s) 232
Tru9I TTAA 1 cut(s) 232
TseI GCWGC 4 cut(s) 47, 50, 53, 76
VpaK11BI GGWCC 2 cut(s) 99, 192
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.