Rh2CG308000

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007999
Physical Location & Seq
Forward (+)
38375746 .. 38380348
4603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG308000.1

Sequence Viewer

Length: 735 bp
ATGGCCTCCCCCTCAAGGGCCATCGCATTCAAATGTTCTCCCTCTCTGGCATTTTCTTCGAAAACGCGGATGGACTCGAAACCCAGAACCCTTCAAAACCCATTTTCTAGTTCAACTTCAGGCGCTGCTCCAAGAGAAGCTCGTACAGGCGACACTGCTGAAGCCTCTATCACATTGCAGGAATGGCAGGGTTGGGGCACCACGTCCCCCGTGCCCTCCTTGGTCACCAAAATTGTTCAGGATTTGAAGGTTTTGGAGAAAGATATTGATGCCCAAATGAGCTTTGGTGGCAGTGGTGGCAAACTTCAGGGAGATTTTAAAGTGCAAGAGGATAGAAAACACAGGAAGACATATAAGGCTTTAGGTGACTCTGAACAAAAGATTCAGTTTTTTTCAGCTAGACAAATAGCATGCCGCTTGCTTGGAAGTAGAGGCTACCTTTGTCAAAAGTGCTGGCTTCCTTTGGAAGATTGTATGTGTTCAAGAGTGGTACAGTGCCCTCTTTGGCCTCGAATGCGATTCTGGTTGTATATGCATCCAAAGGATTTCTTAAGACAGAACAATACTGGAAAGTTGTTATGGCAAGTATTTGGCACTGAAGCTGCAACTTTGTGCCTCTTCGGCATCGCTGAACAGGAAGAAATAATGTGGGATGCATTTAAGCTAGCAGGTCTCCCTTGTTTTTCCCTACTTAAACTGATAGGGCCACTAAATGTGGTGTCCTTAAATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

244

Amino Acids

27.3

Weight (kDa)

9.12

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 146 - 219 3.1e-13 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 659
AccB1I GGYRCC 1 cut(s) 197
AccII CGCG 1 cut(s) 67
AciI CCGC 2 cut(s) 67, 415
AcuI CTGAAG 4 cut(s) 102, 180, 290, 618
AfaI GTAC 2 cut(s) 145, 492
AfiI CCNNNNNNNGG 2 cut(s) 15, 16
AflII CTTAAG 1 cut(s) 550
AgsI TTSAA 5 cut(s) 31, 95, 114, 247, 483
AjiI CACGTC 1 cut(s) 204
AluBI AGCT 5 cut(s) 140, 282, 398, 602, 664
AluI AGCT 5 cut(s) 140, 282, 398, 602, 664
Alw26I GTCTC 1 cut(s) 677
AlwNI CAGNNNCTG 1 cut(s) 125
AoxI GGCC 4 cut(s) 3, 18, 506, 704
ApeKI GCWGC 2 cut(s) 125, 602
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 18, 704
AsuHPI GGTGA 2 cut(s) 217, 377
AsuII TTCGAA 1 cut(s) 59
AsuNHI GCTAGC 1 cut(s) 664
BaeGI GKGCMC 3 cut(s) 200, 216, 500
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 112, 589
BccI CCATC 2 cut(s) 29, 64
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BcoDI GTCTC 1 cut(s) 677
BfaI CTAG 3 cut(s) 108, 399, 665
BfoI RGCGCY 1 cut(s) 126
BfrI CTTAAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 659
BglI GCCNNNNNGGC 1 cut(s) 621
BisI GCNGC 3 cut(s) 126, 415, 603
BlsI GCNGC 3 cut(s) 127, 416, 604
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 18, 704
BmiI GGNNCC 1 cut(s) 199
BmsI GCATC 4 cut(s) 259, 544, 633, 643
BmtI GCTAGC 1 cut(s) 668
BpiI GAAGAC 1 cut(s) 353
Bpu14I TTCGAA 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 16
Bse1I ACTGG 1 cut(s) 571
Bse3DI GCAATG 1 cut(s) 173
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 3 cut(s) 75, 535, 658
BseLI CCNNNNNNNGG 2 cut(s) 15, 16
BseMI GCAATG 1 cut(s) 173
BseNI ACTGG 1 cut(s) 571
BseSI GKGCMC 3 cut(s) 200, 216, 500
BseXI GCAGC 2 cut(s) 112, 589
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 4 cut(s) 5, 20, 508, 706
BshNI GGYRCC 1 cut(s) 197
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 2 cut(s) 15, 16
BsmAI GTCTC 1 cut(s) 677
BsmFI GGGAC 1 cut(s) 190
BsmI GAATGC 2 cut(s) 26, 519
BsnI GGCC 4 cut(s) 5, 20, 508, 706
Bso31I GGTCTC 1 cut(s) 677
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 3 cut(s) 200, 216, 500
BspACI CCGC 2 cut(s) 67, 415
BspANI GGCC 4 cut(s) 5, 20, 508, 706
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 659
BspOI GCTAGC 1 cut(s) 668
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 550
BspTNI GGTCTC 1 cut(s) 677
BsrDI GCAATG 1 cut(s) 173
BsrI ACTGG 1 cut(s) 571
BssECI CCNNGG 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 495
Bst6I CTCTTC 1 cut(s) 623
BstAFI CTTAAG 1 cut(s) 550
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 412, 419, 455, 666
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 3 cut(s) 75, 535, 658
BstFNI CGCG 1 cut(s) 67
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstMAI GTCTC 1 cut(s) 677
BstMWI GCNNNNNNNGC 5 cut(s) 184, 288, 297, 514, 621
BstNSI RCATGY 1 cut(s) 414
BstPI GGTNACC 1 cut(s) 223
BstSLI GKGCMC 3 cut(s) 200, 216, 500
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 2 cut(s) 112, 589
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 4 cut(s) 5, 20, 508, 706
BtgZI GCGATG 2 cut(s) 7, 610
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 3 cut(s) 75, 535, 658
BtsI GCAGTG 2 cut(s) 153, 298
BtsIMutI CAGTG 4 cut(s) 153, 298, 500, 594
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 4 cut(s) 412, 419, 455, 666
CaiI CAGNNNCTG 1 cut(s) 125
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 18, 704
Csp6I GTAC 2 cut(s) 144, 491
CviAII CATG 1 cut(s) 411
CviQI GTAC 2 cut(s) 144, 491
DraI TTTAAA 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 623
EarI CTCTTC 1 cut(s) 623
Eco130I CCWWGG 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 677
Eco57I CTGAAG 4 cut(s) 102, 180, 290, 618
Eco91I GGTNACC 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 219
EcoT22I ATGCAT 2 cut(s) 537, 658
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 1 cut(s) 414
FaiI YATR 7 cut(s) 352, 354, 412, 476, 531, 533, 580
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 1 cut(s) 190
FatI CATG 1 cut(s) 410
Fnu4HI GCNGC 3 cut(s) 126, 415, 603
FokI GGATG 3 cut(s) 82, 522, 665
Fsp4HI GCNGC 3 cut(s) 126, 415, 603
FspBI CTAG 3 cut(s) 108, 399, 665
GlaI GCGC 1 cut(s) 124
GluI GCNGC 3 cut(s) 126, 415, 603
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 4 cut(s) 5, 20, 508, 706
HhaI GCGC 1 cut(s) 125
Hin1II CATG 1 cut(s) 414
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HinfI GANTC 4 cut(s) 74, 368, 382, 519
HphI GGTGA 2 cut(s) 217, 377
Hpy188I TCNGA 1 cut(s) 373
Hpy188III TCNNGA 2 cut(s) 239, 483
HpyAV CCTTC 2 cut(s) 101, 241
HpyCH4III ACNGT 1 cut(s) 495
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 5 cut(s) 178, 325, 535, 605, 656
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 288, 297, 514, 621
HpySE526I ACGT 1 cut(s) 203
Hsp92II CATG 1 cut(s) 414
HspAI GCGC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 2 cut(s) 112, 589
LweI GCATC 4 cut(s) 259, 544, 633, 643
MaeI CTAG 3 cut(s) 108, 399, 665
MaeII ACGT 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 223, 365
MboII GAAGA 5 cut(s) 48, 358, 479, 610, 650
MhlI GDGCHC 3 cut(s) 200, 216, 500
MluCI AATT 2 cut(s) 231, 727
MlyI GAGTC 2 cut(s) 68, 362
Mph1103I ATGCAT 2 cut(s) 537, 658
MseI TTAA 5 cut(s) 318, 551, 660, 693, 725
MslI CAYNNNNRTG 1 cut(s) 31
MspCI CTTAAG 1 cut(s) 550
Mva1269I GAATGC 2 cut(s) 26, 519
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 5 cut(s) 184, 288, 297, 514, 621
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 1 cut(s) 414
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 223, 365
NsiI ATGCAT 2 cut(s) 537, 658
NspI RCATGY 1 cut(s) 414
NspV TTCGAA 1 cut(s) 59
PaeI GCATGC 1 cut(s) 414
PctI GAATGC 2 cut(s) 26, 519
PfeI GAWTC 2 cut(s) 382, 519
PkrI GCNGC 3 cut(s) 127, 416, 604
PleI GAGTC 2 cut(s) 68, 362
PpsI GAGTC 2 cut(s) 68, 362
PspEI GGTNACC 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 199
PspPI GGNCC 2 cut(s) 18, 704
PstNI CAGNNNCTG 1 cut(s) 125
RsaI GTAC 2 cut(s) 145, 492
RsaNI GTAC 2 cut(s) 144, 491
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 5 cut(s) 318, 551, 660, 693, 725
SatI GCNGC 3 cut(s) 126, 415, 603
Sau96I GGNCC 2 cut(s) 18, 704
SchI GAGTC 2 cut(s) 68, 362
SduI GDGCHC 3 cut(s) 200, 216, 500
SfaNI GCATC 4 cut(s) 259, 544, 633, 643
SfuI TTCGAA 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 31
SmlI CTYRAG 2 cut(s) 13, 550
SmoI CTYRAG 2 cut(s) 13, 550
SphI GCATGC 1 cut(s) 414
Sse9I AATT 2 cut(s) 231, 727
SsiI CCGC 2 cut(s) 67, 415
SspMI CTAG 3 cut(s) 108, 399, 665
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 495
TaiI ACGT 1 cut(s) 206
TaqI TCGA 3 cut(s) 59, 77, 511
TasI AATT 2 cut(s) 231, 727
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 2 cut(s) 382, 519
Tru1I TTAA 5 cut(s) 318, 551, 660, 693, 725
Tru9I TTAA 5 cut(s) 318, 551, 660, 693, 725
TscAI CASTG 4 cut(s) 160, 298, 500, 601
TseFI GTSAC 2 cut(s) 223, 365
TseI GCWGC 2 cut(s) 125, 602
Tsp45I GTSAC 2 cut(s) 223, 365
TspRI CASTG 4 cut(s) 160, 298, 500, 601
Vha464I CTTAAG 1 cut(s) 550
XceI RCATGY 1 cut(s) 414
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XspI CTAG 3 cut(s) 108, 399, 665
Zsp2I ATGCAT 2 cut(s) 537, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.