Prupe.3G137200_v2.0.a1

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0007999
Physical Location & Seq
Forward (+)
14490050 .. 14495721
5672 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G137200.3

Sequence Viewer

Length: 1161 bp
ATGGCCTCGCCTAGGGCCTTCACATTCAAATGCTCTCCTTCTCTCGCCTTTTCCACCAAGACACTCATGGACTCAAAACCCAGAACCCTCCGCAGCCCATTTTCATGTTCAACTTCTGGCTCCCCTCAAAGAGAAGCTCGTTTAAGCGACGCTGTTGAAGGCGCTATCACATTGCAGGAATGGCAGGGTTGGGGCACCACGTCCCCTCTGCCCGCCATGGTTACGAAAATTGTTGAGGATTTGAAGGCTTTGGAAAAAGATATCGATGCCCAGATGAGCTTTGGTGGTAGTGGTGGCAAACTTCAGGGGGATTTTAAAGTGCAAGAGGATAAAAAGCACCGGAAAACCTATCAGGCTTTAGGTGATTCTGAACAAAAACTCCAGTTCTTTGCAGCTCGACAAATAGCATGTCGTTTGCTGGGAAGTAGGGGCTACCTTTGTCAAAAGTGCTGGCTTCCTTTGGAAGATTGTATGTGTTCAAACGTCACACAATCCACTCTTTGGCATAGAATGAGGTTCTGGTTATATATGCATCCAAAGGATTTCTTGCGACAGAACAACACTGGAAAGTTGTTATGGCAAGTATTTGGCACTGAGGCTGCAACTTTGTGCCTCTTTGGCATTTCCGAACATGAAGAAATAATGTGGAATGCTTTGAAGCTAGCAGGAAAAAAAAATGTTTGGTGCCTTTATCCCAACAAAAATGCAGCGTTAAAATCAGTTGAGGATGTCTTCGGCCAAGAACCGTCACCATATCTGGAATGCACAGATACTAAGACAAATGAAGATGGAACACTGAATTTCATTTTGATTGATGGAACTTGGAACAATTCAGTTTCGATATTCAGTCGTCTGAAGGATCAAGCAACGTCAGTTTGGGGAGAGGCAGACTTTCCTTGTATTTCCCTGGCCACTGGTGTATCTGCGATGCATAAACTTAGGCCTCAACCATCATGGGATCGTACCTGTACAGCAGGAGCGGCTATTGGCCTCCTCTCTGAGCTGCAACTTCTTCCGGAGTTCAGCTCCTTTGGATTCGATAAACAGGCTGAGGCTCTAGAGGATACTTTAGTTATATTATTAGAAGCACTGACAACTAGAAGAGTTCGGATGGGCAGGTCCATCACTCGTAAAGTAAGACACATGAGTGGCATCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

387

Amino Acids

43.23

Weight (kDa)

8.5

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1107
AccB1I GGYRCC 2 cut(s) 194, 684
AccB7I CCANNNNNTGG 1 cut(s) 501
AccBSI CCGCTC 1 cut(s) 980
AccIII TCCGGA 1 cut(s) 1015
AciI CCGC 3 cut(s) 91, 213, 980
AclWI GGATC 2 cut(s) 867, 966
AcoI YGGCCR 2 cut(s) 736, 909
AcsI RAATTY 1 cut(s) 799
AcuI CTGAAG 2 cut(s) 287, 875
AfaI GTAC 2 cut(s) 964, 970
AfiI CCNNNNNNNGG 2 cut(s) 12, 501
AgsI TTSAA 6 cut(s) 28, 111, 158, 244, 480, 658
AjiI CACGTC 1 cut(s) 201
AjnI CCWGG 1 cut(s) 906
AleI CACNNNNGTG 1 cut(s) 1146
AloI GAACNNNNNNTCC 2 cut(s) 363, 395
AluBI AGCT 6 cut(s) 137, 279, 395, 661, 1003, 1026
AluI AGCT 6 cut(s) 137, 279, 395, 661, 1003, 1026
AlwI GGATC 2 cut(s) 867, 966
Aor13HI TCCGGA 1 cut(s) 1015
AoxI GGCC 6 cut(s) 3, 15, 736, 909, 941, 988
ApeKI GCWGC 5 cut(s) 93, 392, 599, 707, 1003
ApoI RAATTY 1 cut(s) 799
ArsI GACNNNNNNTTYG 2 cut(s) 394, 426
AspA2I CCTAGG 1 cut(s) 11
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 15, 1119
AsuHPI GGTGA 2 cut(s) 374, 741
AsuNHI GCTAGC 1 cut(s) 661
AvaII GGWCC 1 cut(s) 1119
AvrII CCTAGG 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 197
BaeI ACNNNNGTAYC 4 cut(s) 903, 936, 1056, 1089
BalI TGGCCA 1 cut(s) 911
BanI GGYRCC 2 cut(s) 194, 684
BbsI GAAGAC 1 cut(s) 724
BbvCI CCTCAGC 1 cut(s) 1050
BbvI GCAGC 5 cut(s) 105, 404, 586, 719, 990
BccI CCATC 5 cut(s) 782, 809, 958, 1105, 1130
BciT130I CCWGG 1 cut(s) 908
BciVI GTATCC 1 cut(s) 1057
BfaI CTAG 4 cut(s) 12, 662, 1058, 1098
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 1107
BfuI GTATCC 1 cut(s) 1057
BglI GCCNNNNNGGC 1 cut(s) 618
BisI GCNGC 6 cut(s) 94, 393, 600, 708, 981, 1004
BlnI CCTAGG 1 cut(s) 11
BlsI GCNGC 6 cut(s) 95, 394, 601, 709, 982, 1005
Bme1390I CCNGG 1 cut(s) 908
Bme18I GGWCC 1 cut(s) 1119
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 2 cut(s) 15, 1119
BmiI GGNNCC 3 cut(s) 121, 196, 686
BmrFI CCNGG 1 cut(s) 908
BmsI GCATC 3 cut(s) 256, 541, 918
BmtI GCTAGC 1 cut(s) 665
BpiI GAAGAC 1 cut(s) 724
BplI GAGNNNNNCTC 2 cut(s) 1010, 1042
BpmI CTGGAG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 1050
Bsa29I ATCGAT 1 cut(s) 264
BsaJI CCNNGG 3 cut(s) 11, 216, 906
BsaWI WCCGGW 2 cut(s) 339, 1015
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 501
Bse1I ACTGG 3 cut(s) 382, 568, 919
Bse3DI GCAATG 1 cut(s) 170
BseAI TCCGGA 1 cut(s) 1015
BseBI CCWGG 1 cut(s) 908
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 11, 216, 906
BseGI GGATG 3 cut(s) 532, 733, 1116
BseLI CCNNNNNNNGG 2 cut(s) 12, 501
BseMI GCAATG 1 cut(s) 170
BseMII CTCAG 3 cut(s) 585, 990, 1041
BseNI ACTGG 3 cut(s) 382, 568, 919
BseRI GAGGAG 1 cut(s) 983
BseSI GKGCMC 1 cut(s) 197
BseXI GCAGC 5 cut(s) 105, 404, 586, 719, 990
BseYI CCCAGC 1 cut(s) 418
BshFI GGCC 6 cut(s) 5, 17, 738, 911, 943, 990
BshNI GGYRCC 2 cut(s) 194, 684
BshVI ATCGAT 1 cut(s) 264
BsiSI CCGG 2 cut(s) 340, 1016
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 2 cut(s) 12, 501
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 2 cut(s) 655, 767
BsnI GGCC 6 cut(s) 5, 17, 738, 911, 943, 990
Bsp1286I GDGCHC 1 cut(s) 197
Bsp13I TCCGGA 1 cut(s) 1015
Bsp1407I TGTACA 1 cut(s) 968
Bsp143I GATC 2 cut(s) 859, 958
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 3 cut(s) 91, 213, 980
BspANI GGCC 6 cut(s) 5, 17, 738, 911, 943, 990
BspCNI CTCAG 3 cut(s) 586, 991, 1042
BspDI ATCGAT 1 cut(s) 264
BspEI TCCGGA 1 cut(s) 1015
BspLI GGNNCC 3 cut(s) 121, 196, 686
BspMI ACCTGC 1 cut(s) 1107
BspOI GCTAGC 1 cut(s) 665
BspPI GGATC 2 cut(s) 867, 966
BspT107I GGYRCC 2 cut(s) 194, 684
BsrBI CCGCTC 1 cut(s) 980
BsrDI GCAATG 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 968
BsrI ACTGG 3 cut(s) 382, 568, 919
BssECI CCNNGG 3 cut(s) 11, 216, 906
BssMI GATC 2 cut(s) 859, 958
BssT1I CCWWGG 2 cut(s) 11, 216
Bst2UI CCWGG 1 cut(s) 908
Bst4CI ACNGT 1 cut(s) 747
Bst6I CTCTTC 1 cut(s) 1096
BstAUI TGTACA 1 cut(s) 968
BstC8I GCNNGC 3 cut(s) 213, 452, 663
BstDEI CTNAG 5 cut(s) 594, 774, 938, 999, 1050
BstDSI CCRYGG 1 cut(s) 216
BstENI CCTNNNNNAGG 1 cut(s) 10
BstF5I GGATG 3 cut(s) 532, 733, 1116
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 2 cut(s) 862, 961
BstMBI GATC 2 cut(s) 859, 958
BstMWI GCNNNNNNNGC 3 cut(s) 181, 618, 980
BstNI CCWGG 1 cut(s) 908
BstNSI RCATGY 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 906
BstSLI GKGCMC 1 cut(s) 197
BstV1I GCAGC 5 cut(s) 105, 404, 586, 719, 990
BstV2I GAAGAC 1 cut(s) 724
Bsu15I ATCGAT 1 cut(s) 264
BsuI GTATCC 1 cut(s) 1057
BsuRI GGCC 6 cut(s) 5, 17, 738, 911, 943, 990
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 941
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 3 cut(s) 532, 733, 1116
BtsIMutI CAGTG 5 cut(s) 561, 591, 794, 912, 1088
BveI ACCTGC 1 cut(s) 1107
Cac8I GCNNGC 3 cut(s) 213, 452, 663
CfoI GCGC 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 15, 1119
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 1 cut(s) 158
Csp6I GTAC 2 cut(s) 963, 969
CviAII CATG 7 cut(s) 67, 105, 217, 408, 632, 954, 1144
CviQI GTAC 2 cut(s) 963, 969
DdeI CTNAG 5 cut(s) 594, 774, 938, 999, 1050
DpnI GATC 2 cut(s) 861, 960
DpnII GATC 2 cut(s) 859, 958
DraI TTTAAA 1 cut(s) 316
EaeI YGGCCR 2 cut(s) 736, 909
Eam1104I CTCTTC 1 cut(s) 1096
EarI CTCTTC 1 cut(s) 1096
Eco130I CCWWGG 2 cut(s) 11, 216
Eco147I AGGCCT 1 cut(s) 943
Eco32I GATATC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 1119
Eco57I CTGAAG 2 cut(s) 287, 875
EcoNI CCTNNNNNAGG 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 906
EcoRV GATATC 1 cut(s) 262
EcoT14I CCWWGG 2 cut(s) 11, 216
EcoT22I ATGCAT 2 cut(s) 534, 933
ErhI CCWWGG 2 cut(s) 11, 216
FaeI CATG 7 cut(s) 70, 108, 220, 411, 635, 957, 1147
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FaqI GGGAC 1 cut(s) 187
FatI CATG 7 cut(s) 66, 104, 216, 407, 631, 953, 1143
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 6 cut(s) 94, 393, 600, 708, 981, 1004
FokI GGATG 3 cut(s) 519, 740, 1123
Fsp4HI GCNGC 6 cut(s) 94, 393, 600, 708, 981, 1004
FspBI CTAG 4 cut(s) 12, 662, 1058, 1098
GlaI GCGC 1 cut(s) 163
GluI GCNGC 6 cut(s) 94, 393, 600, 708, 981, 1004
GsaI CCCAGC 1 cut(s) 422
GsuI CTGGAG 1 cut(s) 365
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 6 cut(s) 5, 17, 738, 911, 943, 990
HapII CCGG 2 cut(s) 340, 1016
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 164
Hin1II CATG 7 cut(s) 70, 108, 220, 411, 635, 957, 1147
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HinfI GANTC 3 cut(s) 71, 365, 1035
HpaII CCGG 2 cut(s) 340, 1016
HphI GGTGA 2 cut(s) 374, 741
Hpy188I TCNGA 5 cut(s) 370, 628, 855, 1000, 1110
Hpy188III TCNNGA 3 cut(s) 758, 1016, 1058
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 28, 48, 152, 238, 850
HpyCH4III ACNGT 1 cut(s) 747
HpyCH4IV ACGT 3 cut(s) 200, 483, 869
HpyCH4V TGCA 9 cut(s) 175, 322, 392, 532, 602, 707, 765, 931, 1006
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 618, 980
HpyF3I CTNAG 5 cut(s) 594, 774, 938, 999, 1050
HpySE526I ACGT 3 cut(s) 200, 483, 869
Hsp92II CATG 7 cut(s) 70, 108, 220, 411, 635, 957, 1147
HspAI GCGC 1 cut(s) 162
Kpn2I TCCGGA 1 cut(s) 1015
Kzo9I GATC 2 cut(s) 859, 958
LmnI GCTCC 3 cut(s) 125, 977, 1031
Lsp1109I GCAGC 5 cut(s) 105, 404, 586, 719, 990
LweI GCATC 3 cut(s) 256, 541, 918
MaeI CTAG 4 cut(s) 12, 662, 1058, 1098
MaeII ACGT 3 cut(s) 200, 483, 869
MaeIII GTNAC 3 cut(s) 220, 484, 747
MalI GATC 2 cut(s) 861, 960
MbiI CCGCTC 1 cut(s) 980
MboI GATC 2 cut(s) 859, 958
MboII GAAGA 6 cut(s) 476, 647, 724, 797, 1004, 1113
MhlI GDGCHC 1 cut(s) 197
MlsI TGGCCA 1 cut(s) 911
MluCI AATT 3 cut(s) 228, 799, 829
MluNI TGGCCA 1 cut(s) 911
MlyI GAGTC 1 cut(s) 65
Mox20I TGGCCA 1 cut(s) 911
Mph1103I ATGCAT 2 cut(s) 534, 933
MroI TCCGGA 1 cut(s) 1015
MscI TGGCCA 1 cut(s) 911
MseI TTAA 4 cut(s) 143, 315, 713, 1159
MslI CAYNNNNRTG 3 cut(s) 28, 103, 1146
Msp20I TGGCCA 1 cut(s) 911
MspI CCGG 2 cut(s) 340, 1016
MspR9I CCNGG 1 cut(s) 908
Mva1269I GAATGC 2 cut(s) 655, 767
MvaI CCWGG 1 cut(s) 908
MwoI GCNNNNNNNGC 3 cut(s) 181, 618, 980
NcoI CCATGG 1 cut(s) 216
NdeII GATC 2 cut(s) 859, 958
NheI GCTAGC 1 cut(s) 661
NlaIII CATG 7 cut(s) 70, 108, 220, 411, 635, 957, 1147
NlaIV GGNNCC 3 cut(s) 121, 196, 686
NmuCI GTSAC 2 cut(s) 484, 747
NsiI ATGCAT 2 cut(s) 534, 933
NspI RCATGY 1 cut(s) 411
OliI CACNNNNGTG 1 cut(s) 1146
PceI AGGCCT 1 cut(s) 943
PctI GAATGC 2 cut(s) 655, 767
PfeI GAWTC 2 cut(s) 365, 1035
PflMI CCANNNNNTGG 1 cut(s) 501
PkrI GCNGC 6 cut(s) 95, 394, 601, 709, 982, 1005
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 906
PspFI CCCAGC 1 cut(s) 418
PspGI CCWGG 1 cut(s) 906
PspN4I GGNNCC 3 cut(s) 121, 196, 686
PspPI GGNCC 2 cut(s) 15, 1119
RsaI GTAC 2 cut(s) 964, 970
RsaNI GTAC 2 cut(s) 963, 969
RseI CAYNNNNRTG 3 cut(s) 28, 103, 1146
SaqAI TTAA 4 cut(s) 143, 315, 713, 1159
SatI GCNGC 6 cut(s) 94, 393, 600, 708, 981, 1004
Sau3AI GATC 2 cut(s) 859, 958
Sau96I GGNCC 2 cut(s) 15, 1119
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 908
SduI GDGCHC 1 cut(s) 197
SfaNI GCATC 3 cut(s) 256, 541, 918
SinI GGWCC 1 cut(s) 1119
SmiMI CAYNNNNRTG 3 cut(s) 28, 103, 1146
Sse9I AATT 3 cut(s) 228, 799, 829
SseBI AGGCCT 1 cut(s) 943
SsiI CCGC 3 cut(s) 91, 213, 980
SspMI CTAG 4 cut(s) 12, 662, 1058, 1098
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 1 cut(s) 906
StyI CCWWGG 2 cut(s) 11, 216
TaaI ACNGT 1 cut(s) 747
TaiI ACGT 3 cut(s) 203, 486, 872
TaqI TCGA 4 cut(s) 264, 397, 839, 1038
TasI AATT 3 cut(s) 228, 799, 829
TatI WGTACW 1 cut(s) 968
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 2 cut(s) 365, 1035
Tru1I TTAA 4 cut(s) 143, 315, 713, 1159
Tru9I TTAA 4 cut(s) 143, 315, 713, 1159
TscAI CASTG 5 cut(s) 568, 598, 801, 919, 1095
TseFI GTSAC 2 cut(s) 484, 747
TseI GCWGC 5 cut(s) 93, 392, 599, 707, 1003
Tsp45I GTSAC 2 cut(s) 484, 747
TspDTI ATGAA 4 cut(s) 93, 648, 793, 798
TspRI CASTG 5 cut(s) 568, 598, 801, 919, 1095
Van91I CCANNNNNTGG 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 1119
XagI CCTNNNNNAGG 1 cut(s) 10
XapI RAATTY 1 cut(s) 799
XbaI TCTAGA 1 cut(s) 1057
XceI RCATGY 1 cut(s) 411
XcmI CCANNNNNNNNNTGG 2 cut(s) 64, 278
XmaJI CCTAGG 1 cut(s) 11
XspI CTAG 4 cut(s) 12, 662, 1058, 1098
Zsp2I ATGCAT 2 cut(s) 534, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.