MD17G1241000.v1.1

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007999
Physical Location & Seq
Reverse (-)
29008751 .. 29013443
4693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1241000.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGCGTCGCCTTCAAGGGCTTTAACATTGAAATGTTCTCCTTGTATCGCCTTCTCCACCAAAACCCTTATGGACTCCAAACCCACAACTCTCCACAGACCCTTTTCATGTTCAATTTCTGGGTCGCCCCCACAAGAAGCTCGTACAAGCGGCGTTCATGAAGGCGCTATCACATTGCAGGAATGGCAGGGTTGGGGCACCGCGTCTCCAGTTCCCGCCATGGTTACCAAAATTGTTGAGGATTTGAAGGCTTTGGAGAAAGATATTGATACCCAGATGAGCTTTGGTGGTAGTCGTGGCAAACTTCAGGGAGATTTCAAAGTGCAAGAGGATAGAAAACACCGAAAAACATATCAGGCTTTAGGTGATTCTGAAAAAAAGCTCCAATTCTTTTCAGCTCGACAAATAGCATGTCGTTTGCTCGGAAGTAGGGGCTACCTTTGTCAAAAGTGCTGGCTTCCTTCCGAAGAAGATTGTATGTGCTCTAGAGTCACACAATGCACTCTATGGAATAGAATGCGGTTCTGGTTATACATGCATCCCAAGGATTTCTTGCGACAGAACAACACTGGAAAGTTGTTATGGCAGGTATTTGGCACTGAGGCTGCAACTTTGTGCCTCTTTGGCATTTCCGAACATGAAGAAATAATGTGGAATGCGTTGAAGCTTGCAGGAAAAAGTAATACTTGGTGCCTGTATCCCAACAAAAATGGAGTGTTAAAATCAGTTGAGGATGTCTTTGGCCAAGAACCGTCACCATATCTGGCAGGAACAGATACTAAGTTTGACTATGCGAAGACAGATGAAGATAGGACTCTGAATTTCATTTTGATTGACGGAACATGGAACAATTCAGTTGCAATATTCAGTCGGCTGAAGGATCAAGCAGCGTCAGTTTGGGGCGATGGAGACTTTCCTTGTATTTCCCTGGCCACTGGTACTTCTGCAATGCACAAACTTCGGCCCCAACCATCATGGGATCGGACCTGTACAGCAGGAGCGGCTATCGGCCTCCTCTCTGAGCTGCAACTTCTTCCAGAGTTCAGCTCCGTTGGATTAGATAAACAGGCTGAAGCTGTAGAGGATACTTTAGTTGTATTATTAGAAGCACTGACAAATAGACGGCTTCGGATGGGCAGGTCCATCAGTCGCAAAGTACGACACACAATATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

392

Amino Acids

43.72

Weight (kDa)

8.58

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 146 - 375 6.5e-37 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 577, 1128
AccB1I GGYRCC 2 cut(s) 197, 690
AccBSI CCGCTC 1 cut(s) 1001
AccII CGCG 1 cut(s) 203
AciI CCGC 5 cut(s) 150, 201, 216, 520, 1001
AclWI GGATC 2 cut(s) 888, 987
AcoI YGGCCR 2 cut(s) 742, 930
AcsI RAATTY 1 cut(s) 820
AcuI CTGAAG 3 cut(s) 290, 896, 1092
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 145, 940, 991, 1158
AgsI TTSAA 6 cut(s) 15, 31, 114, 247, 319, 664
AjnI CCWGG 1 cut(s) 927
AluBI AGCT 8 cut(s) 140, 282, 382, 398, 667, 1024, 1047, 1076
AluI AGCT 8 cut(s) 140, 282, 382, 398, 667, 1024, 1047, 1076
Alw21I GWGCWC 1 cut(s) 485
Alw26I GTCTC 2 cut(s) 210, 903
AlwI GGATC 2 cut(s) 888, 987
AoxI GGCC 4 cut(s) 742, 930, 962, 1009
ApeKI GCWGC 3 cut(s) 605, 887, 1024
ApoI RAATTY 1 cut(s) 820
ArsI GACNNNNNNTTYG 2 cut(s) 397, 429
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 3 cut(s) 963, 984, 1140
AsuHPI GGTGA 2 cut(s) 377, 747
AvaII GGWCC 2 cut(s) 984, 1140
BaeGI GKGCMC 1 cut(s) 200
BaeI ACNNNNGTAYC 2 cut(s) 1077, 1110
BalI TGGCCA 2 cut(s) 744, 932
BanI GGYRCC 2 cut(s) 197, 690
BbsI GAAGAC 1 cut(s) 803
Bbv12I GWGCWC 1 cut(s) 485
BbvI GCAGC 3 cut(s) 592, 899, 1011
BccI CCATC 4 cut(s) 899, 979, 1126, 1151
BceAI ACGGC 1 cut(s) 1139
BcgI CGANNNNNNTGC 2 cut(s) 941, 975
BciT130I CCWGG 1 cut(s) 929
BciVI GTATCC 2 cut(s) 708, 1078
BcoDI GTCTC 2 cut(s) 210, 903
BfaI CTAG 1 cut(s) 486
BfmI CTRYAG 1 cut(s) 1077
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 2 cut(s) 577, 1128
BfuI GTATCC 2 cut(s) 708, 1078
BglI GCCNNNNNGGC 1 cut(s) 624
BisI GCNGC 5 cut(s) 151, 606, 888, 1002, 1025
BlsI GCNGC 5 cut(s) 152, 607, 889, 1003, 1026
Bme1390I CCNGG 1 cut(s) 929
Bme18I GGWCC 2 cut(s) 984, 1140
BmgT120I GGNCC 3 cut(s) 963, 984, 1140
BmiI GGNNCC 3 cut(s) 199, 692, 965
BmrFI CCNGG 1 cut(s) 929
BmsI GCATC 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 803
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
BpmI CTGGAG 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 219, 543, 927
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bse1I ACTGG 3 cut(s) 209, 574, 940
Bse3DI GCAATG 2 cut(s) 173, 954
BseBI CCWGG 1 cut(s) 929
BseDI CCNNGG 3 cut(s) 219, 543, 927
BseGI GGATG 3 cut(s) 538, 739, 1137
BseMI GCAATG 2 cut(s) 173, 954
BseMII CTCAG 2 cut(s) 591, 1011
BseNI ACTGG 3 cut(s) 209, 574, 940
BseRI GAGGAG 1 cut(s) 1004
BseSI GKGCMC 1 cut(s) 200
BseXI GCAGC 3 cut(s) 592, 899, 1011
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 4 cut(s) 744, 932, 964, 1011
BshNI GGYRCC 2 cut(s) 197, 690
BsiHKAI GWGCWC 1 cut(s) 485
BsmAI GTCTC 2 cut(s) 210, 903
BsmBI CGTCTC 1 cut(s) 210
BsmI GAATGC 2 cut(s) 522, 661
BsnI GGCC 4 cut(s) 744, 932, 964, 1011
Bsp1286I GDGCHC 2 cut(s) 200, 485
Bsp1407I TGTACA 1 cut(s) 989
Bsp143I GATC 2 cut(s) 880, 979
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 5 cut(s) 150, 201, 216, 520, 1001
BspANI GGCC 4 cut(s) 744, 932, 964, 1011
BspCNI CTCAG 2 cut(s) 592, 1012
BspFNI CGCG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 199, 692, 965
BspMI ACCTGC 2 cut(s) 577, 1128
BspPI GGATC 2 cut(s) 888, 987
BspT107I GGYRCC 2 cut(s) 197, 690
BsrBI CCGCTC 1 cut(s) 1001
BsrDI GCAATG 2 cut(s) 173, 954
BsrGI TGTACA 1 cut(s) 989
BsrI ACTGG 3 cut(s) 209, 574, 940
BssECI CCNNGG 3 cut(s) 219, 543, 927
BssMI GATC 2 cut(s) 880, 979
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 219, 543
Bst2UI CCWGG 1 cut(s) 929
Bst4CI ACNGT 1 cut(s) 753
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 989
BstC8I GCNNGC 2 cut(s) 455, 669
BstDEI CTNAG 3 cut(s) 600, 780, 1020
BstDSI CCRYGG 1 cut(s) 219
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 3 cut(s) 538, 739, 1137
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 2 cut(s) 883, 982
BstMAI GTCTC 2 cut(s) 210, 903
BstMBI GATC 2 cut(s) 880, 979
BstMWI GCNNNNNNNGC 3 cut(s) 184, 624, 1001
BstNI CCWGG 1 cut(s) 929
BstNSI RCATGY 2 cut(s) 414, 538
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 927
BstSFI CTRYAG 1 cut(s) 1077
BstSLI GKGCMC 1 cut(s) 200
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 3 cut(s) 592, 899, 1011
BstV2I GAAGAC 1 cut(s) 803
BsuI GTATCC 2 cut(s) 708, 1078
BsuRI GGCC 4 cut(s) 744, 932, 964, 1011
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 918
BtsCI GGATG 3 cut(s) 538, 739, 1137
BtsIMutI CAGTG 4 cut(s) 567, 597, 933, 1109
BveI ACCTGC 2 cut(s) 577, 1128
Cac8I GCNNGC 2 cut(s) 455, 669
CciI TCATGA 1 cut(s) 157
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 963, 984, 1140
CseI GACGC 2 cut(s) 192, 879
Csp6I GTAC 4 cut(s) 144, 939, 990, 1157
CviAII CATG 8 cut(s) 108, 158, 220, 411, 535, 638, 843, 975
CviQI GTAC 4 cut(s) 144, 939, 990, 1157
DdeI CTNAG 3 cut(s) 600, 780, 1020
DpnI GATC 2 cut(s) 882, 981
DpnII GATC 2 cut(s) 880, 979
EaeI YGGCCR 2 cut(s) 742, 930
Eco130I CCWWGG 2 cut(s) 219, 543
Eco47I GGWCC 2 cut(s) 984, 1140
Eco57I CTGAAG 3 cut(s) 290, 896, 1092
Eco91I GGTNACC 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 927
EcoT14I CCWWGG 2 cut(s) 219, 543
EcoT22I ATGCAT 1 cut(s) 540
ErhI CCWWGG 2 cut(s) 219, 543
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 8 cut(s) 111, 161, 223, 414, 538, 641, 846, 978
FalI AAGNNNNNCTT 4 cut(s) 536, 568, 670, 702
FatI CATG 8 cut(s) 107, 157, 219, 410, 534, 637, 842, 974
FauI CCCGC 1 cut(s) 223
Fnu4HI GCNGC 5 cut(s) 151, 606, 888, 1002, 1025
FokI GGATG 3 cut(s) 525, 746, 1144
Fsp4HI GCNGC 5 cut(s) 151, 606, 888, 1002, 1025
FspBI CTAG 1 cut(s) 486
GlaI GCGC 1 cut(s) 166
GluI GCNGC 5 cut(s) 151, 606, 888, 1002, 1025
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 4 cut(s) 744, 932, 964, 1011
HgaI GACGC 2 cut(s) 192, 879
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 111, 161, 223, 414, 538, 641, 846, 978
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 4 cut(s) 74, 368, 489, 814
HphI GGTGA 2 cut(s) 377, 747
Hpy188I TCNGA 8 cut(s) 373, 425, 466, 634, 819, 984, 1021, 1131
Hpy188III TCNNGA 3 cut(s) 158, 486, 1037
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 6 cut(s) 21, 61, 155, 241, 471, 871
HpyCH4III ACNGT 1 cut(s) 753
HpyF10VI GCNNNNNNNGC 3 cut(s) 184, 624, 1001
HpyF3I CTNAG 3 cut(s) 600, 780, 1020
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 111, 161, 223, 414, 538, 641, 846, 978
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 880, 979
LmnI GCTCC 3 cut(s) 387, 998, 1052
Lsp1109I GCAGC 3 cut(s) 592, 899, 1011
LweI GCATC 1 cut(s) 547
MaeI CTAG 1 cut(s) 486
MaeIII GTNAC 3 cut(s) 223, 490, 753
MalI GATC 2 cut(s) 882, 981
MbiI CCGCTC 1 cut(s) 1001
MboI GATC 2 cut(s) 880, 979
MboII GAAGA 6 cut(s) 479, 482, 653, 808, 818, 1025
MhlI GDGCHC 2 cut(s) 200, 485
MlsI TGGCCA 2 cut(s) 744, 932
MluCI AATT 5 cut(s) 114, 231, 386, 820, 850
MluNI TGGCCA 2 cut(s) 744, 932
MlyI GAGTC 3 cut(s) 68, 498, 808
MmeI TCCRAC 1 cut(s) 1033
MnlI CCTC 8 cut(s) 232, 322, 595, 629, 724, 1022, 1025, 1075
Mox20I TGGCCA 2 cut(s) 744, 932
Mph1103I ATGCAT 1 cut(s) 540
MscI TGGCCA 2 cut(s) 744, 932
MseI TTAA 3 cut(s) 23, 719, 1174
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 2 cut(s) 744, 932
MspR9I CCNGG 1 cut(s) 929
Mva1269I GAATGC 2 cut(s) 522, 661
MvaI CCWGG 1 cut(s) 929
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 3 cut(s) 184, 624, 1001
NcoI CCATGG 1 cut(s) 219
NdeII GATC 2 cut(s) 880, 979
NlaIII CATG 8 cut(s) 111, 161, 223, 414, 538, 641, 846, 978
NlaIV GGNNCC 3 cut(s) 199, 692, 965
NmuCI GTSAC 2 cut(s) 490, 753
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 2 cut(s) 414, 538
PagI TCATGA 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 1156
PctI GAATGC 2 cut(s) 522, 661
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 5 cut(s) 152, 607, 889, 1003, 1026
PleI GAGTC 3 cut(s) 68, 497, 808
PpsI GAGTC 3 cut(s) 68, 497, 808
Psp6I CCWGG 1 cut(s) 927
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 1 cut(s) 927
PspN4I GGNNCC 3 cut(s) 199, 692, 965
PspPI GGNCC 3 cut(s) 963, 984, 1140
RsaI GTAC 4 cut(s) 145, 940, 991, 1158
RsaNI GTAC 4 cut(s) 144, 939, 990, 1157
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 3 cut(s) 23, 719, 1174
SatI GCNGC 5 cut(s) 151, 606, 888, 1002, 1025
Sau3AI GATC 2 cut(s) 880, 979
Sau96I GGNCC 3 cut(s) 963, 984, 1140
SchI GAGTC 3 cut(s) 68, 498, 808
ScrFI CCNGG 1 cut(s) 929
SduI GDGCHC 2 cut(s) 200, 485
SfaNI GCATC 1 cut(s) 547
SfcI CTRYAG 1 cut(s) 1077
SinI GGWCC 2 cut(s) 984, 1140
SmiMI CAYNNNNRTG 1 cut(s) 31
Sse9I AATT 5 cut(s) 114, 231, 386, 820, 850
SsiI CCGC 5 cut(s) 150, 201, 216, 520, 1001
SspI AATATT 1 cut(s) 864
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 927
StyI CCWWGG 2 cut(s) 219, 543
TaaI ACNGT 1 cut(s) 753
TaqI TCGA 1 cut(s) 400
TasI AATT 5 cut(s) 114, 231, 386, 820, 850
TatI WGTACW 1 cut(s) 989
TauI GCSGC 2 cut(s) 153, 1004
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 3 cut(s) 23, 719, 1174
Tru9I TTAA 3 cut(s) 23, 719, 1174
TscAI CASTG 4 cut(s) 574, 604, 940, 1116
TseFI GTSAC 2 cut(s) 490, 753
TseI GCWGC 3 cut(s) 605, 887, 1024
Tsp45I GTSAC 2 cut(s) 490, 753
TspDTI ATGAA 6 cut(s) 96, 146, 174, 654, 814, 819
TspGWI ACGGA 2 cut(s) 852, 1039
TspRI CASTG 4 cut(s) 574, 604, 940, 1116
VpaK11BI GGWCC 2 cut(s) 984, 1140
XapI RAATTY 1 cut(s) 820
XbaI TCTAGA 1 cut(s) 485
XceI RCATGY 2 cut(s) 414, 538
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 281
XspI CTAG 1 cut(s) 486
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.