RLG00000018904

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007999
Physical Location & Seq
Forward (+)
42527263 .. 42530966
3704 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018904

Sequence Viewer

Length: 1152 bp
ATGGCCTCCCCCTCAAGGGCCATCGCATTCAAATGTTCTCCCTCTCTGGCATTTTCTTCGAAAACGCGGATGGACTCGAAACCCAGAACCCTTCAAAACCCATTTTCTAGTTCAACTTCAAGGGAAGCTCGTACAGGCGACGCTGCTGAAGCCTCAATCACATTGCAGGAATGGCAGGGTTGGGGCACCAGCTCCCCCGTCCCCGCCATGGTCACCAAAATTGTTCAGGATTTGAAGGTTTTGGAGAAAGATATTGATGCCCAAATGAGCTTTGGTGGCAGTGGTGGCAAGCTTCAGGGAGATTTTAAAGTGCAAGAGGATAGAAAACACAGGAAGACATATAAGGCTTTAGGTGACTCTGAACAAAAGATTCAGTTTTTTTCAGCTAGACAAATAGCATGCCGCTTGCTTGGAAGTAAAGGCTACCTTTGTCAAAAGTGCTGGCTTCCTTTGGAAGATTGTATGTGTTCAAGAGTGGTACTGTGCTCTCTTTGGCCTCGAATGCGATTCTGGTTGTATATGCATCCAAAGGATTTCTTAAGACAGAACAATACTGGAAAGTTGTTATGGCAAGTATTTGGCACTGAAGCTGCAACTTTGTGCCTCTTCGGCATTGCTGAACAGGAAGAAATAATGTGGGATGCATTTAAGCTAGCAGGAAAAAGCAATGTTTGGTGCCTCTATCCCAACAAAAATGCTATGTTAACTTCTGTTCAGGATGTCTTTGGCCAAGAATCTTCAGCTGATCTGGAAGGTCCATATACCAAGATAAAAGAAGACAGAACTCTGAATTTCATTTTGATTGATGGAACATGGAACAATTCTGTTGCGATATTCCATCGTCTGAAGGATCAAGCAAAGTTAGTATGGGGAGGAGAGGACTTTCCTTGTATTTCCCTGGCCACTGGTGCTTCTGCGATGCATAAACTTCGGCCCCAACCATCATTGGATCGTACCTGTACAGCAGCAGCAGCTATTGGCCTCCTCTCCGAGCTGCACGTTCTTCCAGAGTTCAGGTCCCATGGATTTGATAAGCAGGCTGAAGCTGTAGAAGATGCTTTAGTTGTACTATTAGAGGCTCTAACAAATAGACGACTTCGGATGGGCAGGTCCATCAGTCGTAAAGTAAGACAGACGAGCGATTTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

384

Amino Acids

42.9

Weight (kDa)

8.97

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 142 - 365 2e-38 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1098
AccB1I GGYRCC 2 cut(s) 185, 675
AccII CGCG 1 cut(s) 67
AciI CCGC 3 cut(s) 67, 204, 403
AclWI GGATC 2 cut(s) 858, 957
AcoI YGGCCR 2 cut(s) 727, 900
AcsI RAATTY 1 cut(s) 790
AcuI CTGAAG 6 cut(s) 168, 278, 606, 723, 866, 1062
AfaI GTAC 5 cut(s) 133, 480, 955, 961, 1068
AfiI CCNNNNNNNGG 3 cut(s) 15, 16, 208
AflII CTTAAG 1 cut(s) 538
AgsI TTSAA 6 cut(s) 31, 95, 114, 120, 235, 471
AjnI CCWGG 1 cut(s) 897
Alw21I GWGCWC 1 cut(s) 488
AlwI GGATC 2 cut(s) 858, 957
AoxI GGCC 7 cut(s) 3, 18, 494, 727, 900, 932, 979
ApeKI GCWGC 6 cut(s) 143, 590, 965, 968, 971, 994
ApoI RAATTY 1 cut(s) 790
AspS9I GGNCC 5 cut(s) 18, 755, 933, 1017, 1110
AsuHPI GGTGA 2 cut(s) 205, 365
AsuII TTCGAA 1 cut(s) 59
AsuNHI GCTAGC 1 cut(s) 652
AvaII GGWCC 3 cut(s) 755, 1017, 1110
BaeGI GKGCMC 1 cut(s) 188
BalI TGGCCA 2 cut(s) 729, 902
BanI GGYRCC 2 cut(s) 185, 675
BbsI GAAGAC 2 cut(s) 341, 783
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 6 cut(s) 130, 577, 977, 980, 981, 983
BccI CCATC 7 cut(s) 29, 64, 800, 846, 949, 1096, 1121
BcgI CGANNNNNNTGC 4 cut(s) 39, 73, 911, 945
BciT130I CCWGG 1 cut(s) 899
BfaI CTAG 3 cut(s) 108, 387, 653
BfmI CTRYAG 1 cut(s) 1047
BfrI CTTAAG 1 cut(s) 538
BfuAI ACCTGC 1 cut(s) 1098
BglI GCCNNNNNGGC 1 cut(s) 609
BisI GCNGC 7 cut(s) 144, 403, 591, 966, 969, 972, 995
BlsI GCNGC 7 cut(s) 145, 404, 592, 967, 970, 973, 996
Bme1390I CCNGG 1 cut(s) 899
Bme18I GGWCC 3 cut(s) 755, 1017, 1110
BmgT120I GGNCC 5 cut(s) 18, 755, 933, 1017, 1110
BmiI GGNNCC 4 cut(s) 187, 677, 935, 1019
BmrFI CCNGG 1 cut(s) 899
BmsI GCATC 5 cut(s) 247, 532, 631, 909, 1045
BmtI GCTAGC 1 cut(s) 656
BpiI GAAGAC 2 cut(s) 341, 783
Bpu14I TTCGAA 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 207, 897, 1021
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 16, 208
Bse1I ACTGG 2 cut(s) 559, 910
Bse3DI GCAATG 3 cut(s) 161, 612, 673
BseBI CCWGG 1 cut(s) 899
BseDI CCNNGG 3 cut(s) 207, 897, 1021
BseGI GGATG 5 cut(s) 75, 523, 646, 724, 1107
BseLI CCNNNNNNNGG 3 cut(s) 15, 16, 208
BseMI GCAATG 3 cut(s) 161, 612, 673
BseNI ACTGG 2 cut(s) 559, 910
BseRI GAGGAG 2 cut(s) 888, 974
BseSI GKGCMC 1 cut(s) 188
BseXI GCAGC 6 cut(s) 130, 577, 977, 980, 981, 983
BsgI GTGCAG 1 cut(s) 980
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 7 cut(s) 5, 20, 496, 729, 902, 934, 981
BshNI GGYRCC 2 cut(s) 185, 675
BsiHKAI GWGCWC 1 cut(s) 488
BslFI GGGAC 2 cut(s) 185, 1003
BslI CCNNNNNNNGG 3 cut(s) 15, 16, 208
BsmFI GGGAC 2 cut(s) 185, 1003
BsmI GAATGC 2 cut(s) 26, 507
BsnI GGCC 7 cut(s) 5, 20, 496, 729, 902, 934, 981
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 188, 488
Bsp1407I TGTACA 1 cut(s) 959
Bsp143I GATC 3 cut(s) 745, 850, 949
Bsp19I CCATGG 2 cut(s) 207, 1021
BspACI CCGC 3 cut(s) 67, 204, 403
BspANI GGCC 7 cut(s) 5, 20, 496, 729, 902, 934, 981
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 4 cut(s) 187, 677, 935, 1019
BspMI ACCTGC 1 cut(s) 1098
BspOI GCTAGC 1 cut(s) 656
BspPI GGATC 2 cut(s) 858, 957
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 185, 675
BspTI CTTAAG 1 cut(s) 538
BsrDI GCAATG 3 cut(s) 161, 612, 673
BsrGI TGTACA 1 cut(s) 959
BsrI ACTGG 2 cut(s) 559, 910
BssECI CCNNGG 3 cut(s) 207, 897, 1021
BssMI GATC 3 cut(s) 745, 850, 949
BssT1I CCWWGG 2 cut(s) 207, 1021
Bst2UI CCWGG 1 cut(s) 899
Bst4CI ACNGT 1 cut(s) 483
Bst6I CTCTTC 1 cut(s) 611
BstAFI CTTAAG 1 cut(s) 538
BstAUI TGTACA 1 cut(s) 959
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 6 cut(s) 290, 400, 407, 443, 654, 1038
BstDSI CCRYGG 2 cut(s) 207, 1021
BstEII GGTNACC 1 cut(s) 211
BstF5I GGATG 5 cut(s) 75, 523, 646, 724, 1107
BstFNI CGCG 1 cut(s) 67
BstKTI GATC 3 cut(s) 748, 853, 952
BstMBI GATC 3 cut(s) 745, 850, 949
BstMWI GCNNNNNNNGC 8 cut(s) 149, 172, 276, 285, 502, 609, 908, 971
BstNI CCWGG 1 cut(s) 899
BstNSI RCATGY 1 cut(s) 402
BstPI GGTNACC 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 897
BstSFI CTRYAG 1 cut(s) 1047
BstSLI GKGCMC 1 cut(s) 188
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 6 cut(s) 130, 577, 977, 980, 981, 983
BstV2I GAAGAC 2 cut(s) 341, 783
BsuRI GGCC 7 cut(s) 5, 20, 496, 729, 902, 934, 981
BtgI CCRYGG 2 cut(s) 207, 1021
BtgZI GCGATG 2 cut(s) 7, 932
BtsCI GGATG 5 cut(s) 75, 523, 646, 724, 1107
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 3 cut(s) 286, 582, 903
BveI ACCTGC 1 cut(s) 1098
Cac8I GCNNGC 6 cut(s) 290, 400, 407, 443, 654, 1038
Cfr13I GGNCC 5 cut(s) 18, 755, 933, 1017, 1110
CseI GACGC 1 cut(s) 149
Csp6I GTAC 5 cut(s) 132, 479, 954, 960, 1067
CviAII CATG 4 cut(s) 208, 399, 813, 1022
CviQI GTAC 5 cut(s) 132, 479, 954, 960, 1067
DpnI GATC 3 cut(s) 747, 852, 951
DpnII GATC 3 cut(s) 745, 850, 949
DraI TTTAAA 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 727, 900
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
Eco130I CCWWGG 2 cut(s) 207, 1021
Eco47I GGWCC 3 cut(s) 755, 1017, 1110
Eco57I CTGAAG 6 cut(s) 168, 278, 606, 723, 866, 1062
Eco91I GGTNACC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 1017
EcoO65I GGTNACC 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 897
EcoT14I CCWWGG 2 cut(s) 207, 1021
EcoT22I ATGCAT 3 cut(s) 525, 646, 924
ErhI CCWWGG 2 cut(s) 207, 1021
FaeI CATG 4 cut(s) 211, 402, 816, 1025
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 521, 553
FaqI GGGAC 2 cut(s) 185, 1003
FatI CATG 4 cut(s) 207, 398, 812, 1021
FauI CCCGC 1 cut(s) 211
Fnu4HI GCNGC 7 cut(s) 144, 403, 591, 966, 969, 972, 995
FokI GGATG 5 cut(s) 82, 510, 653, 731, 1114
Fsp4HI GCNGC 7 cut(s) 144, 403, 591, 966, 969, 972, 995
FspBI CTAG 3 cut(s) 108, 387, 653
GluI GCNGC 7 cut(s) 144, 403, 591, 966, 969, 972, 995
HaeIII GGCC 7 cut(s) 5, 20, 496, 729, 902, 934, 981
HgaI GACGC 1 cut(s) 149
Hin1II CATG 4 cut(s) 211, 402, 816, 1025
HincII GTYRAC 1 cut(s) 705
HindII GTYRAC 1 cut(s) 705
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 5 cut(s) 74, 356, 370, 507, 734
HpaI GTTAAC 1 cut(s) 705
HphI GGTGA 2 cut(s) 205, 365
Hpy166II GTNNAC 1 cut(s) 705
Hpy188I TCNGA 5 cut(s) 361, 789, 846, 991, 1101
Hpy188III TCNNGA 5 cut(s) 227, 471, 716, 749, 1007
Hpy8I GTNNAC 1 cut(s) 705
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 4 cut(s) 101, 229, 746, 841
HpyCH4III ACNGT 1 cut(s) 483
HpyCH4IV ACGT 1 cut(s) 999
HpyCH4V TGCA 7 cut(s) 166, 313, 523, 593, 644, 922, 997
HpyF10VI GCNNNNNNNGC 8 cut(s) 149, 172, 276, 285, 502, 609, 908, 971
HpySE526I ACGT 1 cut(s) 999
Hsp92II CATG 4 cut(s) 211, 402, 816, 1025
KspAI GTTAAC 1 cut(s) 705
Kzo9I GATC 3 cut(s) 745, 850, 949
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 6 cut(s) 130, 577, 977, 980, 981, 983
LweI GCATC 5 cut(s) 247, 532, 631, 909, 1045
MaeI CTAG 3 cut(s) 108, 387, 653
MaeII ACGT 1 cut(s) 999
MaeIII GTNAC 2 cut(s) 211, 353
MalI GATC 3 cut(s) 747, 852, 951
MboI GATC 3 cut(s) 745, 850, 949
MboII GAAGA 9 cut(s) 48, 346, 467, 598, 638, 729, 788, 995, 1064
MhlI GDGCHC 2 cut(s) 188, 488
MlsI TGGCCA 2 cut(s) 729, 902
MluCI AATT 3 cut(s) 219, 790, 820
MluNI TGGCCA 2 cut(s) 729, 902
MlyI GAGTC 2 cut(s) 68, 350
Mox20I TGGCCA 2 cut(s) 729, 902
Mph1103I ATGCAT 3 cut(s) 525, 646, 924
MscI TGGCCA 2 cut(s) 729, 902
MseI TTAA 5 cut(s) 306, 539, 648, 704, 1150
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 2 cut(s) 729, 902
MspA1I CMGCKG 1 cut(s) 743
MspCI CTTAAG 1 cut(s) 538
MspR9I CCNGG 1 cut(s) 899
Mva1269I GAATGC 2 cut(s) 26, 507
MvaI CCWGG 1 cut(s) 899
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 8 cut(s) 149, 172, 276, 285, 502, 609, 908, 971
NcoI CCATGG 2 cut(s) 207, 1021
NdeII GATC 3 cut(s) 745, 850, 949
NheI GCTAGC 1 cut(s) 652
NlaIII CATG 4 cut(s) 211, 402, 816, 1025
NlaIV GGNNCC 4 cut(s) 187, 677, 935, 1019
NmuCI GTSAC 2 cut(s) 211, 353
NsiI ATGCAT 3 cut(s) 525, 646, 924
NspI RCATGY 1 cut(s) 402
NspV TTCGAA 1 cut(s) 59
PaeI GCATGC 1 cut(s) 402
PctI GAATGC 2 cut(s) 26, 507
PfeI GAWTC 3 cut(s) 370, 507, 734
PkrI GCNGC 7 cut(s) 145, 404, 592, 967, 970, 973, 996
PleI GAGTC 2 cut(s) 68, 350
PpsI GAGTC 2 cut(s) 68, 350
PpuMI RGGWCCY 1 cut(s) 1017
Psp5II RGGWCCY 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 897
PspEI GGTNACC 1 cut(s) 211
PspGI CCWGG 1 cut(s) 897
PspN4I GGNNCC 4 cut(s) 187, 677, 935, 1019
PspPI GGNCC 5 cut(s) 18, 755, 933, 1017, 1110
PspPPI RGGWCCY 1 cut(s) 1017
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 5 cut(s) 133, 480, 955, 961, 1068
RsaNI GTAC 5 cut(s) 132, 479, 954, 960, 1067
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 5 cut(s) 306, 539, 648, 704, 1150
SatI GCNGC 7 cut(s) 144, 403, 591, 966, 969, 972, 995
Sau3AI GATC 3 cut(s) 745, 850, 949
Sau96I GGNCC 5 cut(s) 18, 755, 933, 1017, 1110
SchI GAGTC 2 cut(s) 68, 350
ScrFI CCNGG 1 cut(s) 899
SduI GDGCHC 2 cut(s) 188, 488
SfaNI GCATC 5 cut(s) 247, 532, 631, 909, 1045
SfcI CTRYAG 1 cut(s) 1047
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 3 cut(s) 755, 1017, 1110
SmiMI CAYNNNNRTG 1 cut(s) 31
SmlI CTYRAG 2 cut(s) 13, 538
SmoI CTYRAG 2 cut(s) 13, 538
SphI GCATGC 1 cut(s) 402
Sse9I AATT 3 cut(s) 219, 790, 820
SsiI CCGC 3 cut(s) 67, 204, 403
SspMI CTAG 3 cut(s) 108, 387, 653
StyD4I CCNGG 1 cut(s) 897
StyI CCWWGG 2 cut(s) 207, 1021
TaaI ACNGT 1 cut(s) 483
TaiI ACGT 1 cut(s) 1002
TaqI TCGA 3 cut(s) 59, 77, 499
TasI AATT 3 cut(s) 219, 790, 820
TatI WGTACW 2 cut(s) 959, 1066
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 3 cut(s) 370, 507, 734
Tru1I TTAA 5 cut(s) 306, 539, 648, 704, 1150
Tru9I TTAA 5 cut(s) 306, 539, 648, 704, 1150
TscAI CASTG 3 cut(s) 286, 589, 910
TseFI GTSAC 2 cut(s) 211, 353
TseI GCWGC 6 cut(s) 143, 590, 965, 968, 971, 994
Tsp45I GTSAC 2 cut(s) 211, 353
TspDTI ATGAA 1 cut(s) 784
TspRI CASTG 3 cut(s) 286, 589, 910
Vha464I CTTAAG 1 cut(s) 538
VpaK11BI GGWCC 3 cut(s) 755, 1017, 1110
XapI RAATTY 1 cut(s) 790
XceI RCATGY 1 cut(s) 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 269
XspI CTAG 3 cut(s) 108, 387, 653
Zsp2I ATGCAT 3 cut(s) 525, 646, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.