Rw2G025930

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007999
Physical Location & Seq
Forward (+)
39593898 .. 39598290
4393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025930.1

Sequence Viewer

Length: 1164 bp
ATGGCCTCCCCCTCAAGGGCCATCGCATTCAAATGTTCTCCCTCTCTGGCATTTTCTTCGAAAACGCGGATGGACTCGAAACCCAGAACCCTTCAAAACCCATTTTCTAGTTCAACTTCAGGCGCTGCTCCAAGAGAAGCTCGTACAGGCGACACTGCTGAAGCCTCTATCACATTGCAGGAATGGCAGGGTTGGGGCACCACGTCCCCCGTGCCCTCCTTGGTCACCAAAATTGTTCAGGATTTGAAGGTTTTGGAGAAAGATATTGATGCCCAAATGAGCTTTGGTGGCAGTGGTGGCAAACTTCAGGGAGATTTTAAAGTGCAAGAGGATAGAAAACACAGGAAGACATATAAGGCTTTAGGTGACTCTGAACAAAAGATTCAGTTTTTTTCAGCTAGACAAATAGCATGCCGCTTGCTTGGAAGTAGAGGCTACCTTTGTCAAAAGTGCTGGCTTCCTTTGGAAGATTGTATGTGTTCAAGAGTGGTACAGTGCCCTCTTTGGCCTCGAATGCGATTCTGGTTGTATATGCATCCAAAGGATTTCTTAAGACAGAACAATACTGGAAAGTTGTTATGGCAAGTATTTGGCACTGAAGCTGCAACGTTGTGCCTCTTCGGCATCGCTGAACAGGAAGAAATAATGTGGGATGCATTTAAGCTAGCAGGAAAAAGCAATGTTTGGTGCCTCTATCCCAACAAAAATGCTGTGTTAACTTCTGTTCAGGATGTCTTTGGCCAAGAATCTTCAGCTGATCTGGAAGGTCCATATACCCAGATAAATGAAGACAGAACTCTGAATTTCATTTTGATTGATGGAACATGGAACAATTCTGTTGCGATATTCCGTCGTCTGAAGGATCAAGCAAAGTTAGTATGGGGAGGAGAGGACTTTCCTTGTATTTCCCTGGCCACTGGTGCTTCTGCGATGCATAAACTTCGGCCCCAACCATCATTGGATCGTACCTGTACAGCAGCAGCAGCTATTGGCCTCCTCTCCGAGCTGCACGTTCTTCCAGAGTTCAGGTCCCATGGATTTGATAAGCAGGCTGAAGTTGTAGAGGATGCTTTAGTTGTACTATTAGAGGCTCTAACAAATAGACGACTTCGGATGGGCAGGTCCATCAGTCGTAAAGTAAGACATACGAGCGATTTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

387

Amino Acids

43.3

Weight (kDa)

8.91

Isoelectric Point (pI)

48.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 146 - 369 8.4e-39 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1110
AccB1I GGYRCC 2 cut(s) 197, 687
AccII CGCG 1 cut(s) 67
AciI CCGC 2 cut(s) 67, 415
AclI AACGTT 1 cut(s) 608
AclWI GGATC 2 cut(s) 870, 969
AcoI YGGCCR 2 cut(s) 739, 912
AcsI RAATTY 1 cut(s) 802
AcuI CTGAAG 7 cut(s) 102, 180, 290, 618, 735, 878, 1074
AfaI GTAC 5 cut(s) 145, 492, 967, 973, 1080
AfiI CCNNNNNNNGG 2 cut(s) 15, 16
AflII CTTAAG 1 cut(s) 550
AgsI TTSAA 5 cut(s) 31, 95, 114, 247, 483
AjiI CACGTC 1 cut(s) 204
AjnI CCWGG 1 cut(s) 909
AluBI AGCT 8 cut(s) 140, 282, 398, 602, 664, 755, 986, 1006
AluI AGCT 8 cut(s) 140, 282, 398, 602, 664, 755, 986, 1006
AlwI GGATC 2 cut(s) 870, 969
AlwNI CAGNNNCTG 1 cut(s) 125
AoxI GGCC 7 cut(s) 3, 18, 506, 739, 912, 944, 991
ApeKI GCWGC 6 cut(s) 125, 602, 977, 980, 983, 1006
ApoI RAATTY 1 cut(s) 802
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 5 cut(s) 18, 767, 945, 1029, 1122
AsuHPI GGTGA 2 cut(s) 217, 377
AsuII TTCGAA 1 cut(s) 59
AsuNHI GCTAGC 1 cut(s) 664
AvaII GGWCC 3 cut(s) 767, 1029, 1122
BaeGI GKGCMC 3 cut(s) 200, 216, 500
BalI TGGCCA 2 cut(s) 741, 914
BanI GGYRCC 2 cut(s) 197, 687
BbsI GAAGAC 2 cut(s) 353, 795
BbvI GCAGC 6 cut(s) 112, 589, 989, 992, 993, 995
BccI CCATC 6 cut(s) 29, 64, 812, 961, 1108, 1133
BcgI CGANNNNNNTGC 4 cut(s) 39, 73, 923, 957
BciT130I CCWGG 1 cut(s) 911
BfaI CTAG 3 cut(s) 108, 399, 665
BfoI RGCGCY 1 cut(s) 126
BfrI CTTAAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 1110
BglI GCCNNNNNGGC 1 cut(s) 621
BisI GCNGC 7 cut(s) 126, 415, 603, 978, 981, 984, 1007
BlsI GCNGC 7 cut(s) 127, 416, 604, 979, 982, 985, 1008
Bme1390I CCNGG 1 cut(s) 911
Bme18I GGWCC 3 cut(s) 767, 1029, 1122
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 5 cut(s) 18, 767, 945, 1029, 1122
BmiI GGNNCC 4 cut(s) 199, 689, 947, 1031
BmrFI CCNGG 1 cut(s) 911
BmsI GCATC 6 cut(s) 259, 544, 633, 643, 921, 1057
BmtI GCTAGC 1 cut(s) 668
BpiI GAAGAC 2 cut(s) 353, 795
Bpu14I TTCGAA 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 219, 909, 1033
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 16
Bse1I ACTGG 2 cut(s) 571, 922
Bse3DI GCAATG 2 cut(s) 173, 685
BseBI CCWGG 1 cut(s) 911
BseDI CCNNGG 3 cut(s) 219, 909, 1033
BseGI GGATG 6 cut(s) 75, 535, 658, 736, 1072, 1119
BseLI CCNNNNNNNGG 2 cut(s) 15, 16
BseMI GCAATG 2 cut(s) 173, 685
BseNI ACTGG 2 cut(s) 571, 922
BseRI GAGGAG 2 cut(s) 900, 986
BseSI GKGCMC 3 cut(s) 200, 216, 500
BseXI GCAGC 6 cut(s) 112, 589, 989, 992, 993, 995
BsgI GTGCAG 1 cut(s) 992
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 7 cut(s) 5, 20, 508, 741, 914, 946, 993
BshNI GGYRCC 2 cut(s) 197, 687
BslFI GGGAC 2 cut(s) 190, 1015
BslI CCNNNNNNNGG 2 cut(s) 15, 16
BsmFI GGGAC 2 cut(s) 190, 1015
BsmI GAATGC 2 cut(s) 26, 519
BsnI GGCC 7 cut(s) 5, 20, 508, 741, 914, 946, 993
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 3 cut(s) 200, 216, 500
Bsp1407I TGTACA 1 cut(s) 971
Bsp143I GATC 3 cut(s) 757, 862, 961
Bsp19I CCATGG 1 cut(s) 1033
BspACI CCGC 2 cut(s) 67, 415
BspANI GGCC 7 cut(s) 5, 20, 508, 741, 914, 946, 993
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 4 cut(s) 199, 689, 947, 1031
BspMI ACCTGC 1 cut(s) 1110
BspOI GCTAGC 1 cut(s) 668
BspPI GGATC 2 cut(s) 870, 969
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 197, 687
BspTI CTTAAG 1 cut(s) 550
BsrDI GCAATG 2 cut(s) 173, 685
BsrGI TGTACA 1 cut(s) 971
BsrI ACTGG 2 cut(s) 571, 922
BssECI CCNNGG 3 cut(s) 219, 909, 1033
BssMI GATC 3 cut(s) 757, 862, 961
BssT1I CCWWGG 2 cut(s) 219, 1033
Bst2UI CCWGG 1 cut(s) 911
Bst4CI ACNGT 1 cut(s) 495
Bst6I CTCTTC 1 cut(s) 623
BstAFI CTTAAG 1 cut(s) 550
BstAUI TGTACA 1 cut(s) 971
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 5 cut(s) 412, 419, 455, 666, 1050
BstDSI CCRYGG 1 cut(s) 1033
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 6 cut(s) 75, 535, 658, 736, 1072, 1119
BstFNI CGCG 1 cut(s) 67
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 3 cut(s) 760, 865, 964
BstMBI GATC 3 cut(s) 757, 862, 961
BstMWI GCNNNNNNNGC 8 cut(s) 184, 288, 297, 514, 621, 920, 983, 1158
BstNI CCWGG 1 cut(s) 911
BstNSI RCATGY 1 cut(s) 414
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 909
BstSLI GKGCMC 3 cut(s) 200, 216, 500
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 6 cut(s) 112, 589, 989, 992, 993, 995
BstV2I GAAGAC 2 cut(s) 353, 795
BsuRI GGCC 7 cut(s) 5, 20, 508, 741, 914, 946, 993
BtgI CCRYGG 1 cut(s) 1033
BtgZI GCGATG 3 cut(s) 7, 610, 944
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 6 cut(s) 75, 535, 658, 736, 1072, 1119
BtsI GCAGTG 2 cut(s) 153, 298
BtsIMutI CAGTG 5 cut(s) 153, 298, 500, 594, 915
BveI ACCTGC 1 cut(s) 1110
Cac8I GCNNGC 5 cut(s) 412, 419, 455, 666, 1050
CaiI CAGNNNCTG 1 cut(s) 125
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 5 cut(s) 18, 767, 945, 1029, 1122
Csp6I GTAC 5 cut(s) 144, 491, 966, 972, 1079
CviAII CATG 3 cut(s) 411, 825, 1034
CviQI GTAC 5 cut(s) 144, 491, 966, 972, 1079
DpnI GATC 3 cut(s) 759, 864, 963
DpnII GATC 3 cut(s) 757, 862, 961
DraI TTTAAA 1 cut(s) 319
EaeI YGGCCR 2 cut(s) 739, 912
Eam1104I CTCTTC 1 cut(s) 623
EarI CTCTTC 1 cut(s) 623
Eco130I CCWWGG 2 cut(s) 219, 1033
Eco47I GGWCC 3 cut(s) 767, 1029, 1122
Eco57I CTGAAG 7 cut(s) 102, 180, 290, 618, 735, 878, 1074
Eco91I GGTNACC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 1029
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 909
EcoT14I CCWWGG 2 cut(s) 219, 1033
EcoT22I ATGCAT 3 cut(s) 537, 658, 936
ErhI CCWWGG 2 cut(s) 219, 1033
FaeI CATG 3 cut(s) 414, 828, 1037
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 2 cut(s) 190, 1015
FatI CATG 3 cut(s) 410, 824, 1033
Fnu4HI GCNGC 7 cut(s) 126, 415, 603, 978, 981, 984, 1007
FokI GGATG 6 cut(s) 82, 522, 665, 743, 1079, 1126
Fsp4HI GCNGC 7 cut(s) 126, 415, 603, 978, 981, 984, 1007
FspBI CTAG 3 cut(s) 108, 399, 665
GlaI GCGC 1 cut(s) 124
GluI GCNGC 7 cut(s) 126, 415, 603, 978, 981, 984, 1007
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 7 cut(s) 5, 20, 508, 741, 914, 946, 993
HhaI GCGC 1 cut(s) 125
Hin1II CATG 3 cut(s) 414, 828, 1037
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 5 cut(s) 74, 368, 382, 519, 746
HpaI GTTAAC 1 cut(s) 717
HphI GGTGA 2 cut(s) 217, 377
Hpy166II GTNNAC 1 cut(s) 717
Hpy188I TCNGA 5 cut(s) 373, 801, 858, 1003, 1113
Hpy188III TCNNGA 5 cut(s) 239, 483, 728, 761, 1019
Hpy8I GTNNAC 1 cut(s) 717
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 4 cut(s) 101, 241, 758, 853
HpyCH4III ACNGT 1 cut(s) 495
HpyCH4IV ACGT 3 cut(s) 203, 608, 1011
HpyCH4V TGCA 7 cut(s) 178, 325, 535, 605, 656, 934, 1009
HpyF10VI GCNNNNNNNGC 8 cut(s) 184, 288, 297, 514, 621, 920, 983, 1158
HpySE526I ACGT 3 cut(s) 203, 608, 1011
Hsp92II CATG 3 cut(s) 414, 828, 1037
HspAI GCGC 1 cut(s) 123
KspAI GTTAAC 1 cut(s) 717
Kzo9I GATC 3 cut(s) 757, 862, 961
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 6 cut(s) 112, 589, 989, 992, 993, 995
LweI GCATC 6 cut(s) 259, 544, 633, 643, 921, 1057
MaeI CTAG 3 cut(s) 108, 399, 665
MaeII ACGT 3 cut(s) 203, 608, 1011
MaeIII GTNAC 2 cut(s) 223, 365
MalI GATC 3 cut(s) 759, 864, 963
MboI GATC 3 cut(s) 757, 862, 961
MboII GAAGA 8 cut(s) 48, 358, 479, 610, 650, 741, 800, 1007
MhlI GDGCHC 3 cut(s) 200, 216, 500
MlsI TGGCCA 2 cut(s) 741, 914
MluCI AATT 3 cut(s) 231, 802, 832
MluNI TGGCCA 2 cut(s) 741, 914
MlyI GAGTC 2 cut(s) 68, 362
Mox20I TGGCCA 2 cut(s) 741, 914
Mph1103I ATGCAT 3 cut(s) 537, 658, 936
MscI TGGCCA 2 cut(s) 741, 914
MseI TTAA 4 cut(s) 318, 551, 660, 716
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 2 cut(s) 741, 914
MspA1I CMGCKG 1 cut(s) 755
MspCI CTTAAG 1 cut(s) 550
MspR9I CCNGG 1 cut(s) 911
Mva1269I GAATGC 2 cut(s) 26, 519
MvaI CCWGG 1 cut(s) 911
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 8 cut(s) 184, 288, 297, 514, 621, 920, 983, 1158
NcoI CCATGG 1 cut(s) 1033
NdeII GATC 3 cut(s) 757, 862, 961
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 3 cut(s) 414, 828, 1037
NlaIV GGNNCC 4 cut(s) 199, 689, 947, 1031
NmuCI GTSAC 2 cut(s) 223, 365
NsiI ATGCAT 3 cut(s) 537, 658, 936
NspI RCATGY 1 cut(s) 414
NspV TTCGAA 1 cut(s) 59
PaeI GCATGC 1 cut(s) 414
PctI GAATGC 2 cut(s) 26, 519
PfeI GAWTC 3 cut(s) 382, 519, 746
PkrI GCNGC 7 cut(s) 127, 416, 604, 979, 982, 985, 1008
PleI GAGTC 2 cut(s) 68, 362
PpsI GAGTC 2 cut(s) 68, 362
PpuMI RGGWCCY 1 cut(s) 1029
Psp1406I AACGTT 1 cut(s) 608
Psp5II RGGWCCY 1 cut(s) 1029
Psp6I CCWGG 1 cut(s) 909
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 1 cut(s) 909
PspN4I GGNNCC 4 cut(s) 199, 689, 947, 1031
PspPI GGNCC 5 cut(s) 18, 767, 945, 1029, 1122
PspPPI RGGWCCY 1 cut(s) 1029
PstNI CAGNNNCTG 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 5 cut(s) 145, 492, 967, 973, 1080
RsaNI GTAC 5 cut(s) 144, 491, 966, 972, 1079
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 4 cut(s) 318, 551, 660, 716
SatI GCNGC 7 cut(s) 126, 415, 603, 978, 981, 984, 1007
Sau3AI GATC 3 cut(s) 757, 862, 961
Sau96I GGNCC 5 cut(s) 18, 767, 945, 1029, 1122
SchI GAGTC 2 cut(s) 68, 362
ScrFI CCNGG 1 cut(s) 911
SduI GDGCHC 3 cut(s) 200, 216, 500
SfaNI GCATC 6 cut(s) 259, 544, 633, 643, 921, 1057
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 3 cut(s) 767, 1029, 1122
SmiMI CAYNNNNRTG 1 cut(s) 31
SmlI CTYRAG 2 cut(s) 13, 550
SmoI CTYRAG 2 cut(s) 13, 550
SphI GCATGC 1 cut(s) 414
Sse9I AATT 3 cut(s) 231, 802, 832
SsiI CCGC 2 cut(s) 67, 415
SspMI CTAG 3 cut(s) 108, 399, 665
StyD4I CCNGG 1 cut(s) 909
StyI CCWWGG 2 cut(s) 219, 1033
TaaI ACNGT 1 cut(s) 495
TaiI ACGT 3 cut(s) 206, 611, 1014
TaqI TCGA 3 cut(s) 59, 77, 511
TasI AATT 3 cut(s) 231, 802, 832
TatI WGTACW 2 cut(s) 971, 1078
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 3 cut(s) 382, 519, 746
Tru1I TTAA 4 cut(s) 318, 551, 660, 716
Tru9I TTAA 4 cut(s) 318, 551, 660, 716
TscAI CASTG 5 cut(s) 160, 298, 500, 601, 922
TseFI GTSAC 2 cut(s) 223, 365
TseI GCWGC 6 cut(s) 125, 602, 977, 980, 983, 1006
Tsp45I GTSAC 2 cut(s) 223, 365
TspDTI ATGAA 2 cut(s) 796, 801
TspGWI ACGGA 1 cut(s) 839
TspRI CASTG 5 cut(s) 160, 298, 500, 601, 922
Vha464I CTTAAG 1 cut(s) 550
VpaK11BI GGWCC 3 cut(s) 767, 1029, 1122
XapI RAATTY 1 cut(s) 802
XceI RCATGY 1 cut(s) 414
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XspI CTAG 3 cut(s) 108, 399, 665
Zsp2I ATGCAT 3 cut(s) 537, 658, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.