Rh2AG319900

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007999
Physical Location & Seq
Forward (+)
45760595 .. 45764945
4351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG319900.1

Sequence Viewer

Length: 933 bp
ATGGCCTCCCCCTCGAGGGCCATCGCATTCAAATGTTCTCCCTCTCTGGCATTTTCTTCGAAAACGCGGATGGACTCGAAATCCAGAACCCTTCAAAACCCATTTTCTAGTTCAACTTCAGGCGCTGCTCCAAGAGAAGCTCGTACAGGCGACACTGCTGAAGCCTCTATCACATTGCAGGAATGGCAGGGTTGGGGCACCACGTCCCCCGTGCCCTCCTTGGTCACCAAAATTGTTCAGGATTTGAAGGTTTTGGAGAAAGATATTGATGCCCAAATGAGCTTTGGTGGCAGTGGTGGCAAACTTCAGGGAGATTTTAAAGTGCAAGAGGATAGAAAACACAGGAAGACATATAAGGCTTTAGGTGACTCTGAACAAAAGATTCAGTTTTTTTCAGCTAGACAAATAGCATGCCGCTTGCTTGGAAGTAGAGGCTACCTTTGTCAAAAGTGCTGGCTTCCTTTGGAAGATTGTATGTGTTCAAGAGTGGTACAGTGCCCTCTTTGGCCTCGAATGCGATTCTGGTTGTATATGCATCCAAAGGATTTCTTAAGACAGAACAATACTGGAAAGTTGTTATGGCAAGTATTTGGCACTGAAGCTGCAACTTTGTGCCTCTTCGGCATTGCTGAACAGGAAGAAATAATGTGGGATGCATTTAAGCTAGCAGGAAAAAGCAATGTTTGGTGCCTCTATCCCAACAAAAATGCTGTGTTAACTTCTGTTCAGGATGTCTTTGGCCAAGAATCTTCAGCTGATCTGGAAGGTCCATATACCAAGATAAATGAAGACAGAACTCTGAATTTCATTTTGATTGATGGAACATGGAACAATTCTGTTGCGATATTCCGTCGTCTGAAGGTAATACTTTCTGTCATTTATTGGTTCCTACGCTTTTCTTGTAGGTTTTCATTATCGTTTGTAAGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

310

Amino Acids

35.16

Weight (kDa)

9.18

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 146 - 289 1.2e-28 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 13
AccB1I GGYRCC 2 cut(s) 197, 687
AccII CGCG 1 cut(s) 67
AciI CCGC 2 cut(s) 67, 415
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 1 cut(s) 802
AcuI CTGAAG 6 cut(s) 102, 180, 290, 618, 735, 878
AfaI GTAC 2 cut(s) 145, 492
AfiI CCNNNNNNNGG 2 cut(s) 15, 16
AflII CTTAAG 1 cut(s) 550
AgsI TTSAA 5 cut(s) 31, 95, 114, 247, 483
AjiI CACGTC 1 cut(s) 204
AluBI AGCT 6 cut(s) 140, 282, 398, 602, 664, 755
AluI AGCT 6 cut(s) 140, 282, 398, 602, 664, 755
AlwNI CAGNNNCTG 1 cut(s) 125
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 4 cut(s) 3, 18, 506, 739
ApeKI GCWGC 2 cut(s) 125, 602
ApoI RAATTY 1 cut(s) 802
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 18, 767
AsuHPI GGTGA 2 cut(s) 217, 377
AsuII TTCGAA 1 cut(s) 59
AsuNHI GCTAGC 1 cut(s) 664
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 1 cut(s) 767
BaeGI GKGCMC 3 cut(s) 200, 216, 500
BalI TGGCCA 1 cut(s) 741
BanI GGYRCC 2 cut(s) 197, 687
BbsI GAAGAC 2 cut(s) 353, 795
BbvI GCAGC 2 cut(s) 112, 589
BccI CCATC 3 cut(s) 29, 64, 812
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BfaI CTAG 3 cut(s) 108, 399, 665
BfoI RGCGCY 1 cut(s) 126
BfrI CTTAAG 1 cut(s) 550
BglI GCCNNNNNGGC 1 cut(s) 621
BisI GCNGC 3 cut(s) 126, 415, 603
BlsI GCNGC 3 cut(s) 127, 416, 604
Bme18I GGWCC 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 13
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 18, 767
BmiI GGNNCC 3 cut(s) 199, 689, 887
BmsI GCATC 4 cut(s) 259, 544, 643, 917
BmtI GCTAGC 1 cut(s) 668
BpiI GAAGAC 2 cut(s) 353, 795
Bpu14I TTCGAA 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 16
Bse1I ACTGG 1 cut(s) 571
Bse3DI GCAATG 3 cut(s) 173, 624, 685
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 4 cut(s) 75, 535, 658, 736
BseLI CCNNNNNNNGG 2 cut(s) 15, 16
BseMI GCAATG 3 cut(s) 173, 624, 685
BseNI ACTGG 1 cut(s) 571
BseSI GKGCMC 3 cut(s) 200, 216, 500
BseXI GCAGC 2 cut(s) 112, 589
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 4 cut(s) 5, 20, 508, 741
BshNI GGYRCC 2 cut(s) 197, 687
BsiHKCI CYCGRG 1 cut(s) 13
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 2 cut(s) 15, 16
BsmFI GGGAC 1 cut(s) 190
BsmI GAATGC 2 cut(s) 26, 519
BsnI GGCC 4 cut(s) 5, 20, 508, 741
BsoBI CYCGRG 1 cut(s) 13
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 3 cut(s) 200, 216, 500
Bsp143I GATC 1 cut(s) 757
BspACI CCGC 2 cut(s) 67, 415
BspANI GGCC 4 cut(s) 5, 20, 508, 741
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 3 cut(s) 199, 689, 887
BspOI GCTAGC 1 cut(s) 668
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 197, 687
BspTI CTTAAG 1 cut(s) 550
BsrDI GCAATG 3 cut(s) 173, 624, 685
BsrI ACTGG 1 cut(s) 571
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 1 cut(s) 757
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 495
Bst6I CTCTTC 1 cut(s) 623
BstAFI CTTAAG 1 cut(s) 550
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 412, 419, 455, 666
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 4 cut(s) 75, 535, 658, 736
BstFNI CGCG 1 cut(s) 67
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 1 cut(s) 760
BstMBI GATC 1 cut(s) 757
BstMWI GCNNNNNNNGC 5 cut(s) 184, 288, 297, 514, 621
BstNSI RCATGY 1 cut(s) 414
BstPI GGTNACC 1 cut(s) 223
BstSLI GKGCMC 3 cut(s) 200, 216, 500
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 2 cut(s) 112, 589
BstV2I GAAGAC 2 cut(s) 353, 795
BsuRI GGCC 4 cut(s) 5, 20, 508, 741
BtgZI GCGATG 1 cut(s) 7
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 4 cut(s) 75, 535, 658, 736
BtsI GCAGTG 2 cut(s) 153, 298
BtsIMutI CAGTG 4 cut(s) 153, 298, 500, 594
Cac8I GCNNGC 4 cut(s) 412, 419, 455, 666
CaiI CAGNNNCTG 1 cut(s) 125
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 18, 767
Csp6I GTAC 2 cut(s) 144, 491
CviAII CATG 2 cut(s) 411, 825
CviQI GTAC 2 cut(s) 144, 491
DpnI GATC 1 cut(s) 759
DpnII GATC 1 cut(s) 757
DraI TTTAAA 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 1 cut(s) 623
EarI CTCTTC 1 cut(s) 623
Eco130I CCWWGG 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 767
Eco57I CTGAAG 6 cut(s) 102, 180, 290, 618, 735, 878
Eco88I CYCGRG 1 cut(s) 13
Eco91I GGTNACC 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 219
EcoT22I ATGCAT 2 cut(s) 537, 658
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 2 cut(s) 414, 828
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 1 cut(s) 190
FatI CATG 2 cut(s) 410, 824
Fnu4HI GCNGC 3 cut(s) 126, 415, 603
FokI GGATG 4 cut(s) 82, 522, 665, 743
Fsp4HI GCNGC 3 cut(s) 126, 415, 603
FspBI CTAG 3 cut(s) 108, 399, 665
GlaI GCGC 1 cut(s) 124
GluI GCNGC 3 cut(s) 126, 415, 603
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 4 cut(s) 5, 20, 508, 741
HhaI GCGC 1 cut(s) 125
Hin1II CATG 2 cut(s) 414, 828
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 5 cut(s) 74, 368, 382, 519, 746
HpaI GTTAAC 1 cut(s) 717
HphI GGTGA 2 cut(s) 217, 377
Hpy166II GTNNAC 1 cut(s) 717
Hpy188I TCNGA 3 cut(s) 373, 801, 858
Hpy188III TCNNGA 5 cut(s) 84, 239, 483, 728, 761
Hpy8I GTNNAC 1 cut(s) 717
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 4 cut(s) 101, 241, 758, 853
HpyCH4III ACNGT 1 cut(s) 495
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 5 cut(s) 178, 325, 535, 605, 656
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 288, 297, 514, 621
HpySE526I ACGT 1 cut(s) 203
Hsp92II CATG 2 cut(s) 414, 828
HspAI GCGC 1 cut(s) 123
KspAI GTTAAC 1 cut(s) 717
Kzo9I GATC 1 cut(s) 757
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 2 cut(s) 112, 589
LweI GCATC 4 cut(s) 259, 544, 643, 917
MaeI CTAG 3 cut(s) 108, 399, 665
MaeII ACGT 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 223, 365
MalI GATC 1 cut(s) 759
MboI GATC 1 cut(s) 757
MboII GAAGA 7 cut(s) 48, 358, 479, 610, 650, 741, 800
MhlI GDGCHC 3 cut(s) 200, 216, 500
MlsI TGGCCA 1 cut(s) 741
MluCI AATT 3 cut(s) 231, 802, 832
MluNI TGGCCA 1 cut(s) 741
MlyI GAGTC 2 cut(s) 68, 362
Mox20I TGGCCA 1 cut(s) 741
Mph1103I ATGCAT 2 cut(s) 537, 658
MscI TGGCCA 1 cut(s) 741
MseI TTAA 4 cut(s) 318, 551, 660, 716
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 1 cut(s) 741
MspA1I CMGCKG 1 cut(s) 755
MspCI CTTAAG 1 cut(s) 550
Mva1269I GAATGC 2 cut(s) 26, 519
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 5 cut(s) 184, 288, 297, 514, 621
NdeII GATC 1 cut(s) 757
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 2 cut(s) 414, 828
NlaIV GGNNCC 3 cut(s) 199, 689, 887
NmuCI GTSAC 2 cut(s) 223, 365
NsiI ATGCAT 2 cut(s) 537, 658
NspI RCATGY 1 cut(s) 414
NspV TTCGAA 1 cut(s) 59
PaeI GCATGC 1 cut(s) 414
PaeR7I CTCGAG 1 cut(s) 13
PctI GAATGC 2 cut(s) 26, 519
PfeI GAWTC 3 cut(s) 382, 519, 746
PkrI GCNGC 3 cut(s) 127, 416, 604
PleI GAGTC 2 cut(s) 68, 362
PpsI GAGTC 2 cut(s) 68, 362
PspEI GGTNACC 1 cut(s) 223
PspN4I GGNNCC 3 cut(s) 199, 689, 887
PspPI GGNCC 2 cut(s) 18, 767
PspXI VCTCGAGB 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 2 cut(s) 145, 492
RsaNI GTAC 2 cut(s) 144, 491
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 4 cut(s) 318, 551, 660, 716
SatI GCNGC 3 cut(s) 126, 415, 603
Sau3AI GATC 1 cut(s) 757
Sau96I GGNCC 2 cut(s) 18, 767
SchI GAGTC 2 cut(s) 68, 362
SduI GDGCHC 3 cut(s) 200, 216, 500
SfaNI GCATC 4 cut(s) 259, 544, 643, 917
Sfr274I CTCGAG 1 cut(s) 13
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 1 cut(s) 767
SlaI CTCGAG 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 31
SmlI CTYRAG 2 cut(s) 13, 550
SmoI CTYRAG 2 cut(s) 13, 550
SphI GCATGC 1 cut(s) 414
Sse9I AATT 3 cut(s) 231, 802, 832
SsiI CCGC 2 cut(s) 67, 415
SspMI CTAG 3 cut(s) 108, 399, 665
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 495
TaiI ACGT 1 cut(s) 206
TaqI TCGA 4 cut(s) 14, 59, 77, 511
TasI AATT 3 cut(s) 231, 802, 832
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 3 cut(s) 382, 519, 746
Tru1I TTAA 4 cut(s) 318, 551, 660, 716
Tru9I TTAA 4 cut(s) 318, 551, 660, 716
TscAI CASTG 4 cut(s) 160, 298, 500, 601
TseFI GTSAC 2 cut(s) 223, 365
TseI GCWGC 2 cut(s) 125, 602
Tsp45I GTSAC 2 cut(s) 223, 365
TspDTI ATGAA 3 cut(s) 796, 801, 900
TspGWI ACGGA 1 cut(s) 839
TspRI CASTG 4 cut(s) 160, 298, 500, 601
Vha464I CTTAAG 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 767
XapI RAATTY 1 cut(s) 802
XceI RCATGY 1 cut(s) 414
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 13
XspI CTAG 3 cut(s) 108, 399, 665
Zsp2I ATGCAT 2 cut(s) 537, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.