Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0012244

Physical Location & Seq

Genomic Coordinates Reverse (-)

21283487 .. 21286886

Gene Structure 3400 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g27620.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1191 bp

ATGGAAGCAGAAGACGAGTACAGAAGAAAGGCCCGCGTGGCATTGGTGGCCATTGTTGTCCTCGCTTCCCTCGCCGTCGCTTCCTTACTCGTCGCCTTCAGCTACTACTGCTACATTCGCAACAAGCTCGCCAAGCACCTCAACCACACCAAAGGGGCTGGTGCTGACCTGGAGGGCAAAGGTGGTGTTCCGAATCTGAAAACGGCTGCCGGAGACGGAGTTCAGGTGTTTACATTTAAGCAGCTACATTCGGCGACCGGCGGATTCAGCAAGTCGAATGTGGTTGGGAAAGGTGGGTTCGGGTTGGTGTACCGTGGGGTGCTGCATGATGGGAGGAAAGTTGCTATTAAGTTTATGGACCAAGGAGGGAAGCAGGGAGAAGAAGAATTTGAAATGGAGGTGGAGTTGCTAAGTCGGTTGCGTTCTCCATATTTGCTTCCGTTGCTTGGGTATTGTTCAGATAACAGTCGTAAGCTGTTGGTGTATGAGTTCATGGCCAATGGTGGATTGCAGGAACATTTGTATCCTGTGAGTGGTGGTCCTAATGCCCTCTCCACAAAGTTAGACTGGGAAACCCGGTTGAGAATAGCTCTTGAAGCTGCGAAAGGTTTGGAGTATCTCCATGAACAAGTTAGTCCTCCTGTGATTCACAGAGATTTTAAGAGCAGCAACATTCTCTTGGACAAAACATTCCACGCCAAAGTTTCTGATTTCGGATTGGCCAAACTTGGATCTGACAAAGCTGGGGGTTATGTTTCCACCAGAGTGTTGGGCACACAGGGATATGTTGCCCCTGAGTATGCATTAACAGGGCATCTGACAACTAAATCAGATGTGTACAGTTATGGAGTTGTTCTGTTGGAATTGCTAACTGGCAGAGTTCCAGTTGATATGAAGAGACCCCCTGGAGAAGGTGTTCTTGTTTCTTGGGCTTTACCGCAGCTGACAGATAGGGAGCAGGTTGTACGGATCATGGATCCTGAATTGGAGGGTCAATATTCGATGAAGGACGTCATTCAGGTGGCAGCAATTGCTGCTATGTGTGTACAACCAGAGGCAGATTACAGGCCACTGATGGCAGATGTTGTACAGTCACTGGTTCCGCTTGTGAAGATTCACAGTTCAATCTCAAAGGTAGGCAGAAGTTCTAGTTTGCATGCAACAAAATCACCTACACAGAATTCGGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

397

Amino Acids

43.07

Weight (kDa)

8.7

Isoelectric Point (pI)

30.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	90 - 298	9.2e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	92 - 366	2.1e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58690
fragaria_vesca	FvH4_6g27620
malus_domestica	MD17G1265400.v1.1
prunus_persica	Prupe.3G115500_v2.0.a1 Prupe.3G115500_v2.0.a1
pyrus_communis	pycom17g26510
rosa_chinensis	RchiOBHm_Chr2g0130301
rosa_laevigata	RLG00000019133
rosa_multiflora	Rmu_sc0001167.1_g000081 Rmu_sc0001336.1_g000074 Rmu_sc0029344.1_g000001
rosa_roxburghii	Rroxscaffold_2G00114050
rosa_rugosa	Rorug01G0180700 Rorug01G0180800 Rorug02G0291400
rosa_samantha	Rh2CG330200 Rh2DG369400
rosa_wichuraiana	Rw2G027620 Rw5G020570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1014
Acc36I	ACCTGC	1 cut(s)	949
AccII	CGCG	1 cut(s)	36
AciI	CCGC	4 cut(s)	34, 261, 938, 1103
AclWI	GGATC	4 cut(s)	739, 971, 977, 984
AcoI	YGGCCR	3 cut(s)	48, 495, 720
AcsI	RAATTY	2 cut(s)	386, 1180
AcuI	CTGAAG	1 cut(s)	82
AcyI	GRCGYC	1 cut(s)	1011
AfaI	GTAC	6 cut(s)	20, 311, 839, 966, 1047, 1089
AfiI	CCNNNNNNNGG	3 cut(s)	446, 533, 911
AgsI	TTSAA	3 cut(s)	392, 596, 1125
AjnI	CCWGG	2 cut(s)	168, 904
AleI	CACNNNNGTG	1 cut(s)	764
AloI	GAACNNNNNNTCC	4 cut(s)	204, 236, 900, 932
AluBI	AGCT	8 cut(s)	102, 127, 244, 475, 590, 599, 743, 943
AluI	AGCT	8 cut(s)	102, 127, 244, 475, 590, 599, 743, 943
Alw26I	GTCTC	2 cut(s)	207, 892
AlwI	GGATC	4 cut(s)	739, 971, 977, 984
AlwNI	CAGNNNCTG	1 cut(s)	1096
AoxI	GGCC	5 cut(s)	30, 48, 495, 720, 1067
ApeKI	GCWGC	8 cut(s)	206, 241, 322, 599, 666, 940, 1025, 1034
ApoI	RAATTY	2 cut(s)	386, 1180
Asp700I	GAANNNNTTC	1 cut(s)	915
AspS9I	GGNCC	3 cut(s)	31, 358, 539
AsuC2I	CCSGG	1 cut(s)	577
AsuHPI	GGTGA	1 cut(s)	1161
AvaII	GGWCC	2 cut(s)	358, 539
BaeGI	GKGCMC	1 cut(s)	776
BaeI	ACNNNNGTAYC	2 cut(s)	506, 539
BalI	TGGCCA	3 cut(s)	50, 497, 722
BamHI	GGATCC	1 cut(s)	976
BbsI	GAAGAC	1 cut(s)	18
BbvI	GCAGC	8 cut(s)	193, 253, 309, 586, 678, 952, 1021, 1037
BccI	CCATC	2 cut(s)	323, 1069
BceAI	ACGGC	2 cut(s)	59, 219
BcgI	CGANNNNNNTGC	2 cut(s)	109, 143
BciT130I	CCWGG	2 cut(s)	170, 906
BciVI	GTATCC	1 cut(s)	534
BcnI	CCSGG	1 cut(s)	577
BcoDI	GTCTC	2 cut(s)	207, 892
BfaI	CTAG	1 cut(s)	1149
BfuAI	ACCTGC	1 cut(s)	949
BfuI	GTATCC	1 cut(s)	534
BglI	GCCNNNNNGGC	1 cut(s)	38
BisI	GCNGC	8 cut(s)	207, 242, 323, 600, 667, 941, 1026, 1035
BlsI	GCNGC	8 cut(s)	208, 243, 324, 601, 668, 942, 1027, 1036
Bme1390I	CCNGG	3 cut(s)	170, 577, 906
Bme18I	GGWCC	2 cut(s)	358, 539
BmgT120I	GGNCC	3 cut(s)	31, 358, 539
BmiI	GGNNCC	2 cut(s)	978, 1101
BmrFI	CCNGG	3 cut(s)	170, 577, 906
BmrI	ACTGGG	1 cut(s)	577
BmsI	GCATC	1 cut(s)	823
BmuI	ACTGGG	1 cut(s)	577
BpiI	GAAGAC	1 cut(s)	18
BplI	GAGNNNNNCTC	2 cut(s)	574, 606
BpmI	CTGGAG	2 cut(s)	191, 927
BpuMI	CCSGG	1 cut(s)	577
BsaHI	GRCGYC	1 cut(s)	1011
BsaI	GGTCTC	1 cut(s)	892
BsaJI	CCNNGG	3 cut(s)	313, 361, 904
BsaXI	ACNNNNNCTCC	8 cut(s)	204, 210, 234, 240, 389, 419, 900, 930
Bsc4I	CCNNNNNNNGG	3 cut(s)	446, 533, 911
Bse118I	RCCGGY	1 cut(s)	257
Bse1I	ACTGG	4 cut(s)	572, 877, 884, 1101
BseBI	CCWGG	2 cut(s)	170, 906
BseDI	CCNNGG	3 cut(s)	313, 361, 904
BseLI	CCNNNNNNNGG	3 cut(s)	446, 533, 911
BseMII	CTCAG	1 cut(s)	786
BseNI	ACTGG	4 cut(s)	572, 877, 884, 1101
BseSI	GKGCMC	1 cut(s)	776
BseXI	GCAGC	8 cut(s)	193, 253, 309, 586, 678, 952, 1021, 1037
BseYI	CCCAGC	1 cut(s)	743
Bsh1236I	CGCG	1 cut(s)	36
Bsh1285I	CGRYCG	1 cut(s)	258
BshFI	GGCC	5 cut(s)	32, 50, 497, 722, 1069
BsiEI	CGRYCG	1 cut(s)	258
BsiSI	CCGG	3 cut(s)	210, 258, 577
BslI	CCNNNNNNNGG	3 cut(s)	446, 533, 911
BsmAI	GTCTC	2 cut(s)	207, 892
BsmBI	CGTCTC	1 cut(s)	207
BsnI	GGCC	5 cut(s)	32, 50, 497, 722, 1069
Bso31I	GGTCTC	1 cut(s)	892
Bsp1286I	GDGCHC	1 cut(s)	776
Bsp1407I	TGTACA	3 cut(s)	837, 1045, 1087
Bsp143I	GATC	3 cut(s)	731, 969, 976
BspACI	CCGC	4 cut(s)	34, 261, 938, 1103
BspANI	GGCC	5 cut(s)	32, 50, 497, 722, 1069
BspCNI	CTCAG	1 cut(s)	787
BspFNI	CGCG	1 cut(s)	36
BspLI	GGNNCC	2 cut(s)	978, 1101
BspMI	ACCTGC	1 cut(s)	949
BspPI	GGATC	4 cut(s)	739, 971, 977, 984
BspTNI	GGTCTC	1 cut(s)	892
BsrFI	RCCGGY	1 cut(s)	257
BsrGI	TGTACA	3 cut(s)	837, 1045, 1087
BsrI	ACTGG	4 cut(s)	572, 877, 884, 1101
BssAI	RCCGGY	1 cut(s)	257
BssECI	CCNNGG	3 cut(s)	313, 361, 904
BssMI	GATC	3 cut(s)	731, 969, 976
BssNI	GRCGYC	1 cut(s)	1011
BssT1I	CCWWGG	1 cut(s)	361
Bst2UI	CCWGG	2 cut(s)	170, 906
Bst4CI	ACNGT	5 cut(s)	314, 467, 842, 1092, 1121
Bst6I	CTCTTC	1 cut(s)	890
BstACI	GRCGYC	1 cut(s)	1011
BstAPI	GCANNNNNTGC	2 cut(s)	1031, 1034
BstAUI	TGTACA	3 cut(s)	837, 1045, 1087
BstC8I	GCNNGC	3 cut(s)	34, 129, 1158
BstDEI	CTNAG	2 cut(s)	410, 795
BstDSI	CCRYGG	1 cut(s)	313
BstENI	CCTNNNNNAGG	1 cut(s)	909
BstFNI	CGCG	1 cut(s)	36
BstKTI	GATC	3 cut(s)	734, 972, 979
BstMAI	GTCTC	2 cut(s)	207, 892
BstMBI	GATC	3 cut(s)	731, 969, 976
BstMCI	CGRYCG	1 cut(s)	258
BstNI	CCWGG	2 cut(s)	170, 906
BstNSI	RCATGY	1 cut(s)	1160
BstSCI	CCNGG	3 cut(s)	168, 575, 904
BstSLI	GKGCMC	1 cut(s)	776
BstUI	CGCG	1 cut(s)	36
BstV1I	GCAGC	8 cut(s)	193, 253, 309, 586, 678, 952, 1021, 1037
BstV2I	GAAGAC	1 cut(s)	18
BstX2I	RGATCY	2 cut(s)	731, 976
BstXI	CCANNNNNNTGG	1 cut(s)	769
BstYI	RGATCY	2 cut(s)	731, 976
BsuI	GTATCC	1 cut(s)	534
BsuRI	GGCC	5 cut(s)	32, 50, 497, 722, 1069
BtgI	CCRYGG	1 cut(s)	313
BtsIMutI	CAGTG	2 cut(s)	1070, 1094
BveI	ACCTGC	1 cut(s)	949
Cac8I	GCNNGC	3 cut(s)	34, 129, 1158
CaiI	CAGNNNCTG	1 cut(s)	1096
Cfr10I	RCCGGY	1 cut(s)	257
Cfr13I	GGNCC	3 cut(s)	31, 358, 539
Csp6I	GTAC	6 cut(s)	19, 310, 838, 965, 1046, 1088
CviAII	CATG	5 cut(s)	326, 493, 623, 973, 1157
CviQI	GTAC	6 cut(s)	19, 310, 838, 965, 1046, 1088
DdeI	CTNAG	2 cut(s)	410, 795
DpnI	GATC	3 cut(s)	733, 971, 978
DpnII	GATC	3 cut(s)	731, 969, 976
EaeI	YGGCCR	3 cut(s)	48, 495, 720
Eam1104I	CTCTTC	1 cut(s)	890
EarI	CTCTTC	1 cut(s)	890
EciI	GGCGGA	1 cut(s)	276
Eco130I	CCWWGG	1 cut(s)	361
Eco31I	GGTCTC	1 cut(s)	892
Eco47I	GGWCC	2 cut(s)	358, 539
Eco57I	CTGAAG	1 cut(s)	82
EcoNI	CCTNNNNNAGG	1 cut(s)	909
EcoRI	GAATTC	1 cut(s)	1180
EcoRII	CCWGG	2 cut(s)	168, 904
EcoT14I	CCWWGG	1 cut(s)	361
EcoT22I	ATGCAT	1 cut(s)	805
ErhI	CCWWGG	1 cut(s)	361
Esp3I	CGTCTC	1 cut(s)	207
FaeI	CATG	5 cut(s)	329, 496, 626, 976, 1160
FalI	AAGNNNNNCTT	2 cut(s)	903, 935
FatI	CATG	5 cut(s)	325, 492, 622, 972, 1156
FauI	CCCGC	1 cut(s)	41
Fnu4HI	GCNGC	8 cut(s)	207, 242, 323, 600, 667, 941, 1026, 1035
Fsp4HI	GCNGC	8 cut(s)	207, 242, 323, 600, 667, 941, 1026, 1035
FspBI	CTAG	1 cut(s)	1149
GluI	GCNGC	8 cut(s)	207, 242, 323, 600, 667, 941, 1026, 1035
GsaI	CCCAGC	1 cut(s)	747
GsuI	CTGGAG	2 cut(s)	191, 927
HaeIII	GGCC	5 cut(s)	32, 50, 497, 722, 1069
HapII	CCGG	3 cut(s)	210, 258, 577
Hin1I	GRCGYC	1 cut(s)	1011
Hin1II	CATG	5 cut(s)	329, 496, 626, 976, 1160
HinfI	GANTC	4 cut(s)	193, 264, 646, 1114
HpaII	CCGG	3 cut(s)	210, 258, 577
HphI	GGTGA	1 cut(s)	1161
Hpy166II	GTNNAC	4 cut(s)	231, 310, 838, 1046
Hpy188I	TCNGA	8 cut(s)	192, 198, 460, 709, 716, 736, 819, 832
Hpy188III	TCNNGA	2 cut(s)	593, 980
Hpy8I	GTNNAC	4 cut(s)	231, 310, 838, 1046
Hpy99I	CGWCG	2 cut(s)	80, 95
HpyAV	CCTTC	3 cut(s)	106, 905, 1000
HpyCH4III	ACNGT	5 cut(s)	314, 467, 842, 1092, 1121
HpyCH4IV	ACGT	1 cut(s)	1011
HpyCH4V	TGCA	5 cut(s)	325, 511, 803, 1156, 1160
HpyF3I	CTNAG	2 cut(s)	410, 795
HpySE526I	ACGT	1 cut(s)	1011
Hsp92I	GRCGYC	1 cut(s)	1011
Hsp92II	CATG	5 cut(s)	329, 496, 626, 976, 1160
Kzo9I	GATC	3 cut(s)	731, 969, 976
LmnI	GCTCC	1 cut(s)	955
Lsp1109I	GCAGC	8 cut(s)	193, 253, 309, 586, 678, 952, 1021, 1037
LweI	GCATC	1 cut(s)	823
MaeI	CTAG	1 cut(s)	1149
MaeII	ACGT	1 cut(s)	1011
MaeIII	GTNAC	1 cut(s)	1092
MalI	GATC	3 cut(s)	733, 971, 978
MboI	GATC	3 cut(s)	731, 969, 976
MboII	GAAGA	6 cut(s)	23, 36, 392, 395, 907, 1123
MfeI	CAATTG	1 cut(s)	1029
MflI	RGATCY	2 cut(s)	731, 976
MhlI	GDGCHC	1 cut(s)	776
MlsI	TGGCCA	3 cut(s)	50, 497, 722
MluCI	AATT	5 cut(s)	386, 863, 983, 1029, 1180
MluNI	TGGCCA	3 cut(s)	50, 497, 722
MmeI	TCCRAC	1 cut(s)	840
Mox20I	TGGCCA	3 cut(s)	50, 497, 722
Mph1103I	ATGCAT	1 cut(s)	805
MroXI	GAANNNNTTC	1 cut(s)	915
MscI	TGGCCA	3 cut(s)	50, 497, 722
MseI	TTAA	5 cut(s)	237, 348, 660, 806, 1189
MslI	CAYNNNNRTG	2 cut(s)	764, 1019
Msp20I	TGGCCA	3 cut(s)	50, 497, 722
MspA1I	CMGCKG	1 cut(s)	943
MspI	CCGG	3 cut(s)	210, 258, 577
MspR9I	CCNGG	3 cut(s)	170, 577, 906
MunI	CAATTG	1 cut(s)	1029
MvaI	CCWGG	2 cut(s)	170, 906
MvnI	CGCG	1 cut(s)	36
NciI	CCSGG	1 cut(s)	577
NdeII	GATC	3 cut(s)	731, 969, 976
NlaIII	CATG	5 cut(s)	329, 496, 626, 976, 1160
NlaIV	GGNNCC	2 cut(s)	978, 1101
NmuCI	GTSAC	1 cut(s)	1092
NsiI	ATGCAT	1 cut(s)	805
NspI	RCATGY	1 cut(s)	1160
OliI	CACNNNNGTG	1 cut(s)	764
PaeI	GCATGC	1 cut(s)	1160
PdmI	GAANNNNTTC	1 cut(s)	915
PfeI	GAWTC	4 cut(s)	193, 264, 646, 1114
PkrI	GCNGC	8 cut(s)	208, 243, 324, 601, 668, 942, 1027, 1036
Psp6I	CCWGG	2 cut(s)	168, 904
PspFI	CCCAGC	1 cut(s)	743
PspGI	CCWGG	2 cut(s)	168, 904
PspN4I	GGNNCC	2 cut(s)	978, 1101
PspPI	GGNCC	3 cut(s)	31, 358, 539
PstNI	CAGNNNCTG	1 cut(s)	1096
PsuI	RGATCY	2 cut(s)	731, 976
PvuII	CAGCTG	1 cut(s)	943
RsaI	GTAC	6 cut(s)	20, 311, 839, 966, 1047, 1089
RsaNI	GTAC	6 cut(s)	19, 310, 838, 965, 1046, 1088
RseI	CAYNNNNRTG	2 cut(s)	764, 1019
SaqAI	TTAA	5 cut(s)	237, 348, 660, 806, 1189
SatI	GCNGC	8 cut(s)	207, 242, 323, 600, 667, 941, 1026, 1035
Sau3AI	GATC	3 cut(s)	731, 969, 976
Sau96I	GGNCC	3 cut(s)	31, 358, 539
ScrFI	CCNGG	3 cut(s)	170, 577, 906
SduI	GDGCHC	1 cut(s)	776
SfaNI	GCATC	1 cut(s)	823
SinI	GGWCC	2 cut(s)	358, 539
SmiMI	CAYNNNNRTG	2 cut(s)	764, 1019
SphI	GCATGC	1 cut(s)	1160
Sse9I	AATT	5 cut(s)	386, 863, 983, 1029, 1180
SsiI	CCGC	4 cut(s)	34, 261, 938, 1103
SspI	AATATT	1 cut(s)	998
SspMI	CTAG	1 cut(s)	1149
StyD4I	CCNGG	3 cut(s)	168, 575, 904
StyI	CCWWGG	1 cut(s)	361
TaaI	ACNGT	5 cut(s)	314, 467, 842, 1092, 1121
TaiI	ACGT	1 cut(s)	1014
TaqI	TCGA	2 cut(s)	275, 1001
TasI	AATT	5 cut(s)	386, 863, 983, 1029, 1180
TatI	WGTACW	4 cut(s)	18, 837, 1045, 1087
TfiI	GAWTC	4 cut(s)	193, 264, 646, 1114
Tru1I	TTAA	5 cut(s)	237, 348, 660, 806, 1189
Tru9I	TTAA	5 cut(s)	237, 348, 660, 806, 1189
TscAI	CASTG	2 cut(s)	1077, 1101
TseFI	GTSAC	1 cut(s)	1092
TseI	GCWGC	8 cut(s)	206, 241, 322, 599, 666, 940, 1025, 1034
Tsp45I	GTSAC	1 cut(s)	1092
TspDTI	ATGAA	4 cut(s)	481, 639, 908, 1019
TspGWI	ACGGA	3 cut(s)	231, 429, 982
TspRI	CASTG	2 cut(s)	1077, 1101
VpaK11BI	GGWCC	2 cut(s)	358, 539
XagI	CCTNNNNNAGG	1 cut(s)	909
XapI	RAATTY	2 cut(s)	386, 1180
XceI	RCATGY	1 cut(s)	1160
XcmI	CCANNNNNNNNNTGG	1 cut(s)	766
XmnI	GAANNNNTTC	1 cut(s)	915
XspI	CTAG	1 cut(s)	1149
ZraI	GACGTC	1 cut(s)	1012
Zsp2I	ATGCAT	1 cut(s)	805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.