FvH4_6g30240

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008875
Physical Location & Seq
Reverse (-)
23343595 .. 23348313
4719 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30240.t1

Sequence Viewer

Length: 1422 bp
ATGGATGCTTTGCAAGTCATTTCTTCTGCAACACAGATAGTGTCAAACATGGTAGGTGCCATTGCTGCATTAGAACAAGCATCTAGGAACTTAGATGAAGGTCCAAAGAGAATTCGAAGCCTAGAGGAATTTGTCTGTGATCTTGAGAACTTGATTCGCCGACTTAAGCACAAGCATGTCAATAAACTTCATAATCCTCAGTTAGATCACCAAATTCAAAGCTTGAATTCCCTGATAGAACGTCTGCTTCCAAACATCAGCAAGGCCAGGAGAATGGTGTCAAAGAGTAGGTTTAAGAACTTGGCCAAGGTAGTTTGGACTTCCATGGCTGGAGACCCACTTGGGAAGCTTGTGAACTCAATAAAGGATGACTTGAACTGGTGGCTGGAATCACAAAGATTGGTCCACGATGTTGAGAAGACACTCGAGTCGACTGCACAAGACAGCGGTGTTCGACTGAAAATTAGGACTGAACAAGGCTACCCAGTTTCTAGTAAATGCTACTTTGTTAGGAATTTACTGGAAAAGGATGGCTCCCAACGTGTCATTCTCATTGTTGGGCTATCTGGTATTGGGAAGTCATTTCTGGCTCGTCAAGTAGCTTCTGATCCCCCTGAGAAATTTATGGATGGTGCAGTTGAACTAGGCTTTGGCCAATGGTGCAGTAGAGCTGCTTGTAACAGAAGCATAGTTGAATACCAGAGGCGCTTGGCAAGAAAACTTTGTAAATTTCTGGTGCAGATTGGGTTTCGGAAGAAGATTAGAGATGAACATGGTGGAGATCTAGAATATATATGTTGCTTGCTTCAAGAAGCCTTGTATGGGAAGAGCATTCTTATACTTCTTGATGATGTTTGGGAGCAGGACATAGTTGAGCGGTTCACAAAGCTATATGATAATGATTGCAGGTACCTTGTAACAACAAGAAACGAAGCTGTCTATGAAATTACAGAAGCAGAGAAGGTGGAATTAAGCAAAGATGATGTAAAGGAGACAAGCATGGAAGTTCTTCTCTACCATAGCCTCCTTAGCAAAGAAGAGTTACCGCAAGTAGCAGAGAACTTACTTGAGCGCTGCGGTCACCATCCTTTAACAGTTGCGGTTATGGGTAAGGCTCTCAGAAAAGAAACAAGAGCTGATAAATGGGAAAAGGCCATCACCAACTTATCCACATTTGCTACGTGTGCACCTGGTCCTGTATCATATGTCAATGAAAAAGAAGCTGAGAATGCTTTAACCATTTTCGGATCCTTTGAATTCAGTCTAGATGCAATGCCTGGAGACTCTAGAGAGCTTTTTATAGCTTTGTCGGCTCTTTCGTGGGAAGAACCTGTGCCAGAAGCTTGTGTGGAGGCTGTTTGGTCAGTTCTGGGACAGGAGACCCTGTTCCCACTCATAGTCTGCAAGCTTGTAGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

474

Amino Acids

53.47

Weight (kDa)

5.97

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 171 - 321 9.8e-15 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 427
Acc36I ACCTGC 1 cut(s) 897
Acc65I GGTACC 1 cut(s) 909
AccB1I GGYRCC 2 cut(s) 56, 909
AccBSI CCGCTC 1 cut(s) 877
AccI GTMKAC 1 cut(s) 431
AciI CCGC 5 cut(s) 447, 877, 1046, 1077, 1100
AclWI GGATC 3 cut(s) 602, 1242, 1255
AcoI YGGCCR 2 cut(s) 303, 652
AcsI RAATTY 8 cut(s) 111, 128, 213, 226, 514, 620, 728, 1256
AfaI GTAC 1 cut(s) 911
AfeI AGCGCT 1 cut(s) 1073
AfiI CCNNNNNNNGG 1 cut(s) 822
AflII CTTAAG 1 cut(s) 164
AflIII ACRYGT 2 cut(s) 541, 1181
AgsI TTSAA 7 cut(s) 218, 226, 376, 641, 695, 809, 1256
AjnI CCWGG 3 cut(s) 266, 1189, 1276
AjuI GAANNNNNNNTTGG 4 cut(s) 531, 563, 633, 665
AloI GAACNNNNNNTCC 2 cut(s) 1370, 1402
Alw21I GWGCWC 1 cut(s) 1189
Alw26I GTCTC 4 cut(s) 327, 986, 1275, 1373
Alw44I GTGCAC 1 cut(s) 1185
AlwI GGATC 3 cut(s) 602, 1242, 1255
Ama87I CYCGRG 1 cut(s) 425
Aor51HI AGCGCT 1 cut(s) 1073
AoxI GGCC 4 cut(s) 264, 303, 652, 1152
ApaLI GTGCAC 1 cut(s) 1185
ApeKI GCWGC 3 cut(s) 65, 671, 1074
ApoI RAATTY 8 cut(s) 111, 128, 213, 226, 514, 620, 728, 1256
Asp700I GAANNNNTTC 1 cut(s) 1008
Asp718I GGTACC 1 cut(s) 909
AspLEI GCGC 2 cut(s) 708, 1074
AspS9I GGNCC 3 cut(s) 101, 403, 1193
AsuHPI GGTGA 3 cut(s) 200, 1073, 1150
AsuII TTCGAA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 3 cut(s) 101, 403, 1193
BaeGI GKGCMC 1 cut(s) 1189
BalI TGGCCA 2 cut(s) 305, 654
BamHI GGATCC 1 cut(s) 1247
BanI GGYRCC 2 cut(s) 56, 909
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 1189
BbvI GCAGC 3 cut(s) 52, 658, 1061
BccI CCATC 4 cut(s) 524, 623, 1092, 1163
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 3 cut(s) 268, 1191, 1278
BcoDI GTCTC 4 cut(s) 327, 986, 1275, 1373
BfaI CTAG 7 cut(s) 84, 122, 492, 644, 785, 1265, 1287
BfoI RGCGCY 2 cut(s) 709, 1075
BfrI CTTAAG 1 cut(s) 164
BfuAI ACCTGC 1 cut(s) 897
BglII AGATCT 1 cut(s) 781
BisI GCNGC 3 cut(s) 66, 672, 1075
BlsI GCNGC 3 cut(s) 67, 673, 1076
Bme1390I CCNGG 3 cut(s) 268, 1191, 1278
Bme18I GGWCC 3 cut(s) 101, 403, 1193
BmeT110I CYCGRG 1 cut(s) 425
BmgT120I GGNCC 3 cut(s) 101, 403, 1193
BmiI GGNNCC 4 cut(s) 58, 535, 911, 1249
BmrFI CCNGG 3 cut(s) 268, 1191, 1278
BmrI ACTGGG 1 cut(s) 479
BmsI GCATC 2 cut(s) 89, 1258
BmuI ACTGGG 1 cut(s) 479
BpiI GAAGAC 1 cut(s) 425
BpmI CTGGAG 2 cut(s) 351, 1299
Bpu10I CCTNAGC 1 cut(s) 1028
Bpu14I TTCGAA 1 cut(s) 115
BpuEI CTTGAG 2 cut(s) 164, 1088
BsaAI YACGTR 1 cut(s) 1182
BsaI GGTCTC 2 cut(s) 327, 1373
BsaJI CCNNGG 2 cut(s) 306, 324
BsaXI ACNNNNNCTCC 2 cut(s) 1370, 1400
Bsc4I CCNNNNNNNGG 1 cut(s) 822
Bse1I ACTGG 3 cut(s) 383, 485, 525
Bse3DI GCAATG 2 cut(s) 60, 1278
BseBI CCWGG 3 cut(s) 268, 1191, 1278
BseDI CCNNGG 2 cut(s) 306, 324
BseGI GGATG 5 cut(s) 10, 373, 535, 634, 1084
BseLI CCNNNNNNNGG 1 cut(s) 822
BseMI GCAATG 2 cut(s) 60, 1278
BseMII CTCAG 4 cut(s) 212, 606, 1132, 1215
BseNI ACTGG 3 cut(s) 383, 485, 525
BseSI GKGCMC 1 cut(s) 1189
BseXI GCAGC 3 cut(s) 52, 658, 1061
BsgI GTGCAG 4 cut(s) 420, 654, 682, 758
BshFI GGCC 4 cut(s) 266, 305, 654, 1154
BshNI GGYRCC 2 cut(s) 56, 909
BsiHKAI GWGCWC 1 cut(s) 1189
BsiHKCI CYCGRG 1 cut(s) 425
BslFI GGGAC 1 cut(s) 1386
BslI CCNNNNNNNGG 1 cut(s) 822
BsmAI GTCTC 4 cut(s) 327, 986, 1275, 1373
BsmFI GGGAC 1 cut(s) 1386
BsmI GAATGC 2 cut(s) 831, 1234
BsnI GGCC 4 cut(s) 266, 305, 654, 1154
Bso31I GGTCTC 2 cut(s) 327, 1373
BsoBI CYCGRG 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 1189
Bsp143I GATC 5 cut(s) 139, 205, 607, 781, 1247
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 5 cut(s) 447, 877, 1046, 1077, 1100
BspANI GGCC 4 cut(s) 266, 305, 654, 1154
BspCNI CTCAG 4 cut(s) 211, 607, 1131, 1216
BspLI GGNNCC 4 cut(s) 58, 535, 911, 1249
BspMI ACCTGC 1 cut(s) 897
BspPI GGATC 3 cut(s) 602, 1242, 1255
BspQI GCTCTTC 1 cut(s) 821
BspT104I TTCGAA 1 cut(s) 115
BspT107I GGYRCC 2 cut(s) 56, 909
BspTI CTTAAG 1 cut(s) 164
BspTNI GGTCTC 2 cut(s) 327, 1373
BsrBI CCGCTC 1 cut(s) 877
BsrDI GCAATG 2 cut(s) 60, 1278
BsrI ACTGG 3 cut(s) 383, 485, 525
BssECI CCNNGG 2 cut(s) 306, 324
BssMI GATC 5 cut(s) 139, 205, 607, 781, 1247
BssT1I CCWWGG 2 cut(s) 306, 324
Bst2UI CCWGG 3 cut(s) 268, 1191, 1278
Bst4CI ACNGT 1 cut(s) 1096
Bst6I CTCTTC 2 cut(s) 821, 1032
BstAFI CTTAAG 1 cut(s) 164
BstBAI YACGTR 1 cut(s) 1182
BstBI TTCGAA 1 cut(s) 115
BstC8I GCNNGC 2 cut(s) 803, 1406
BstDEI CTNAG 6 cut(s) 91, 198, 615, 1028, 1118, 1224
BstDSI CCRYGG 1 cut(s) 324
BstEII GGTNACC 1 cut(s) 1079
BstF5I GGATG 5 cut(s) 10, 373, 535, 634, 1084
BstH2I RGCGCY 2 cut(s) 709, 1075
BstHHI GCGC 2 cut(s) 708, 1074
BstKTI GATC 5 cut(s) 142, 208, 610, 784, 1250
BstMAI GTCTC 4 cut(s) 327, 986, 1275, 1373
BstMBI GATC 5 cut(s) 139, 205, 607, 781, 1247
BstMWI GCNNNNNNNGC 6 cut(s) 65, 660, 1029, 1184, 1229, 1310
BstNI CCWGG 3 cut(s) 268, 1191, 1278
BstNSI RCATGY 1 cut(s) 179
BstPI GGTNACC 1 cut(s) 1079
BstSCI CCNGG 3 cut(s) 266, 1189, 1276
BstSLI GKGCMC 1 cut(s) 1189
BstV1I GCAGC 3 cut(s) 52, 658, 1061
BstV2I GAAGAC 1 cut(s) 425
BstX2I RGATCY 2 cut(s) 781, 1247
BstXI CCANNNNNNTGG 1 cut(s) 274
BstYI RGATCY 2 cut(s) 781, 1247
BsuRI GGCC 4 cut(s) 266, 305, 654, 1154
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 5 cut(s) 10, 373, 535, 634, 1084
BveI ACCTGC 1 cut(s) 897
Cac8I GCNNGC 2 cut(s) 803, 1406
CfoI GCGC 2 cut(s) 708, 1074
Cfr13I GGNCC 3 cut(s) 101, 403, 1193
CsiI ACCWGGT 1 cut(s) 1189
Csp6I GTAC 1 cut(s) 910
CviAII CATG 5 cut(s) 49, 176, 325, 773, 1000
CviQI GTAC 1 cut(s) 910
DdeI CTNAG 6 cut(s) 91, 198, 615, 1028, 1118, 1224
DpnI GATC 5 cut(s) 141, 207, 609, 783, 1249
DpnII GATC 5 cut(s) 139, 205, 607, 781, 1247
DrdI GACNNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 427
EaeI YGGCCR 2 cut(s) 303, 652
Eam1104I CTCTTC 2 cut(s) 821, 1032
EarI CTCTTC 2 cut(s) 821, 1032
Eco130I CCWWGG 2 cut(s) 306, 324
Eco31I GGTCTC 2 cut(s) 327, 1373
Eco47I GGWCC 3 cut(s) 101, 403, 1193
Eco47III AGCGCT 1 cut(s) 1073
Eco88I CYCGRG 1 cut(s) 425
Eco91I GGTNACC 1 cut(s) 1079
EcoO65I GGTNACC 1 cut(s) 1079
EcoRI GAATTC 3 cut(s) 111, 226, 1256
EcoRII CCWGG 3 cut(s) 266, 1189, 1276
EcoT14I CCWWGG 2 cut(s) 306, 324
ErhI CCWWGG 2 cut(s) 306, 324
FaeI CATG 5 cut(s) 52, 179, 328, 776, 1003
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 1386
FatI CATG 5 cut(s) 48, 175, 324, 772, 999
FauNDI CATATG 1 cut(s) 1204
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 3 cut(s) 66, 672, 1075
FokI GGATG 5 cut(s) 17, 380, 542, 641, 1071
Fsp4HI GCNGC 3 cut(s) 66, 672, 1075
FspBI CTAG 7 cut(s) 84, 122, 492, 644, 785, 1265, 1287
GlaI GCGC 2 cut(s) 707, 1073
GluI GCNGC 3 cut(s) 66, 672, 1075
GsuI CTGGAG 2 cut(s) 351, 1299
HaeII RGCGCY 2 cut(s) 709, 1075
HaeIII GGCC 4 cut(s) 266, 305, 654, 1154
HhaI GCGC 2 cut(s) 708, 1074
Hin1II CATG 5 cut(s) 52, 179, 328, 776, 1003
Hin6I GCGC 2 cut(s) 706, 1072
HinP1I GCGC 2 cut(s) 706, 1072
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HindIII AAGCTT 4 cut(s) 220, 347, 1341, 1406
HinfI GANTC 4 cut(s) 154, 389, 428, 1283
HphI GGTGA 3 cut(s) 200, 1073, 1150
Hpy166II GTNNAC 5 cut(s) 355, 406, 432, 882, 1187
Hpy188I TCNGA 4 cut(s) 607, 753, 1121, 1247
Hpy188III TCNNGA 6 cut(s) 143, 785, 809, 845, 1265, 1287
Hpy8I GTNNAC 5 cut(s) 355, 406, 432, 882, 1187
HpyAV CCTTC 3 cut(s) 92, 955, 1409
HpyCH4III ACNGT 1 cut(s) 1096
HpyCH4IV ACGT 3 cut(s) 241, 541, 1181
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 660, 1029, 1184, 1229, 1310
HpyF3I CTNAG 6 cut(s) 91, 198, 615, 1028, 1118, 1224
HpySE526I ACGT 3 cut(s) 241, 541, 1181
Hsp92II CATG 5 cut(s) 52, 179, 328, 776, 1003
HspAI GCGC 2 cut(s) 706, 1072
KpnI GGTACC 1 cut(s) 913
Kzo9I GATC 5 cut(s) 139, 205, 607, 781, 1247
LguI GCTCTTC 1 cut(s) 821
LmnI GCTCC 2 cut(s) 539, 859
Lsp1109I GCAGC 3 cut(s) 52, 658, 1061
LweI GCATC 2 cut(s) 89, 1258
MabI ACCWGGT 1 cut(s) 1189
MaeI CTAG 7 cut(s) 84, 122, 492, 644, 785, 1265, 1287
MaeII ACGT 3 cut(s) 241, 541, 1181
MaeIII GTNAC 4 cut(s) 677, 916, 1041, 1079
MalI GATC 5 cut(s) 141, 207, 609, 783, 1249
MbiI CCGCTC 1 cut(s) 877
MboI GATC 5 cut(s) 139, 205, 607, 781, 1247
MboII GAAGA 8 cut(s) 15, 430, 766, 769, 838, 1001, 1049, 1337
MflI RGATCY 2 cut(s) 781, 1247
MhlI GDGCHC 1 cut(s) 1189
MlsI TGGCCA 2 cut(s) 305, 654
MluNI TGGCCA 2 cut(s) 305, 654
MlyI GAGTC 2 cut(s) 437, 1277
MnlI CCTC 5 cut(s) 118, 207, 696, 1034, 1345
Mox20I TGGCCA 2 cut(s) 305, 654
MroXI GAANNNNTTC 1 cut(s) 1008
MscI TGGCCA 2 cut(s) 305, 654
MseI TTAA 5 cut(s) 165, 294, 971, 1091, 1235
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 2 cut(s) 305, 654
MspA1I CMGCKG 1 cut(s) 447
MspCI CTTAAG 1 cut(s) 164
MspR9I CCNGG 3 cut(s) 268, 1191, 1278
Mva1269I GAATGC 2 cut(s) 831, 1234
MvaI CCWGG 3 cut(s) 268, 1191, 1278
MwoI GCNNNNNNNGC 6 cut(s) 65, 660, 1029, 1184, 1229, 1310
NcoI CCATGG 1 cut(s) 324
NdeI CATATG 1 cut(s) 1204
NdeII GATC 5 cut(s) 139, 205, 607, 781, 1247
NlaIII CATG 5 cut(s) 52, 179, 328, 776, 1003
NlaIV GGNNCC 4 cut(s) 58, 535, 911, 1249
NmuCI GTSAC 1 cut(s) 1079
NspI RCATGY 1 cut(s) 179
NspV TTCGAA 1 cut(s) 115
PaeR7I CTCGAG 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 821
PcsI WCGNNNNNNNCGW 1 cut(s) 1316
PctI GAATGC 2 cut(s) 831, 1234
PdmI GAANNNNTTC 1 cut(s) 1008
PfeI GAWTC 2 cut(s) 154, 389
PkrI GCNGC 3 cut(s) 67, 673, 1076
PleI GAGTC 2 cut(s) 436, 1277
PpsI GAGTC 2 cut(s) 436, 1277
Ppu21I YACGTR 1 cut(s) 1182
Psp6I CCWGG 3 cut(s) 266, 1189, 1276
PspEI GGTNACC 1 cut(s) 1079
PspGI CCWGG 3 cut(s) 266, 1189, 1276
PspN4I GGNNCC 4 cut(s) 58, 535, 911, 1249
PspPI GGNCC 3 cut(s) 101, 403, 1193
PspXI VCTCGAGB 1 cut(s) 425
PsuI RGATCY 2 cut(s) 781, 1247
RsaI GTAC 1 cut(s) 911
RsaNI GTAC 1 cut(s) 910
RseI CAYNNNNRTG 1 cut(s) 174
SalI GTCGAC 1 cut(s) 430
SapI GCTCTTC 1 cut(s) 821
SaqAI TTAA 5 cut(s) 165, 294, 971, 1091, 1235
SatI GCNGC 3 cut(s) 66, 672, 1075
Sau3AI GATC 5 cut(s) 139, 205, 607, 781, 1247
Sau96I GGNCC 3 cut(s) 101, 403, 1193
SchI GAGTC 2 cut(s) 437, 1277
ScrFI CCNGG 3 cut(s) 268, 1191, 1278
SduI GDGCHC 1 cut(s) 1189
SexAI ACCWGGT 1 cut(s) 1189
SfaNI GCATC 2 cut(s) 89, 1258
Sfr274I CTCGAG 1 cut(s) 425
SfuI TTCGAA 1 cut(s) 115
SinI GGWCC 3 cut(s) 101, 403, 1193
SlaI CTCGAG 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 4 cut(s) 143, 164, 425, 1067
SmoI CTYRAG 4 cut(s) 143, 164, 425, 1067
SsiI CCGC 5 cut(s) 447, 877, 1046, 1077, 1100
SspMI CTAG 7 cut(s) 84, 122, 492, 644, 785, 1265, 1287
StyD4I CCNGG 3 cut(s) 266, 1189, 1276
StyI CCWWGG 2 cut(s) 306, 324
TaaI ACNGT 1 cut(s) 1096
TaiI ACGT 3 cut(s) 244, 544, 1184
TaqI TCGA 4 cut(s) 115, 426, 431, 454
TfiI GAWTC 2 cut(s) 154, 389
Tru1I TTAA 5 cut(s) 165, 294, 971, 1091, 1235
Tru9I TTAA 5 cut(s) 165, 294, 971, 1091, 1235
TseFI GTSAC 1 cut(s) 1079
TseI GCWGC 3 cut(s) 65, 671, 1074
Tsp45I GTSAC 1 cut(s) 1079
TspDTI ATGAA 5 cut(s) 111, 179, 783, 957, 1227
Vha464I CTTAAG 1 cut(s) 164
VneI GTGCAC 1 cut(s) 1185
VpaK11BI GGWCC 3 cut(s) 101, 403, 1193
XapI RAATTY 8 cut(s) 111, 128, 213, 226, 514, 620, 728, 1256
XbaI TCTAGA 3 cut(s) 784, 1264, 1286
XceI RCATGY 1 cut(s) 179
XhoI CTCGAG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 1008
XspI CTAG 7 cut(s) 84, 122, 492, 644, 785, 1265, 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.