Rorug02G0331200

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008875
Physical Location & Seq
Reverse (-)
41049334 .. 41052307
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0331200.1

Sequence Viewer

Length: 1263 bp
ATGGAAGTTAGAATTGATGATTGTGGTGAAACTAGGTATGGGAAGTGGTTCAAGACGAGAATTTTTGGCAAGATCCATGTGTTTGTTCCAAGTACAGAGGGTGCAAAAATGATTTTTTCCAATGATTTTGCTAAGTTCAACAAAGGATATGTGAAATCAATGGCAGATTGTATTGGAGATAAAAGCTTGCTCTGTGTACCACATGAGAACCATAAAAGAATAAGGCGCTTATTATCAGATCCTTTCTCCATGAATTCTTTATCTTCGTTCATTGAGAAATTCGATAAAATGCTGTGTCAGAAACTGAAGAAACTAGAACAAGGTGGGGAAAGCTTTTCGGTGCTTGACTTCAGTATGAGGATGACATTTGATGCAATGTGCTGCATGCTAATGAGTGTCACGGATGAGCCCTTGCTAAGGCAGCTTGAGAAAGACTGCACTGCAGTCTCGGATGGAATCTTATCTGTTCCTGTCATGATTCCAGGCACTAGATATTACAAAGGCATCAAGGCACGCAGACGTCTCATGGAAACTTTTGAAGACATGATTGCCAGAAGGAGGAGTGGGAAGGAATGGCCAGAACCAGAAGATTTCCTACAGTCCATGTTACGAAGAGACTCATACCCTCCCAATGAAAAGCTGCAAGACTTGGAAATCATGGACAACCTAATGACACTTATAATTGCAGGGCAGACAACCACTTCAGCTGCAATGATGTGGAGTGTCAAGTTTCTGGATGAGAACCAACAAGTCCAAGACATTCTTAGGGAGGAACAATTGTCGATAGCCACATCCAAGCCTGAAGGAGCATCAGTTACCCTCGAAGATATTAATAATATGTCCTACTGTTGGAAGGTTGTTAAAGAAACAGTAAGGATGTCAACTGTGGTTATGTGGTATCCTCGCGTTGCACTCAGTGACTGTACTATAGAAGGTTTTGAAATAAAGAAAGGGTGGCATGTGAACATTGACGCGACATGCATACACAACGACCCAGATTTGTACCCGGACCCTTCCAAATTCAATCCTTCTAGATTTGATGAAATGCAAAAGCCATATAGTTTCATACCATTTGGGTCAGGACCCAGGACATGCTTGGGAATCAATATGGCCAAGATGACAATGATGGTCTTTCTTCACCGTCTCACTACTGCATACAGGTGGACAGTCGATGATCTTGATACCAGTCTAGAAAAGAAGGCCCATATTCCTAGACTAAGAAGTGGTTGTCCCATTACCTTGACGGCGCGGCCTTTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

420

Amino Acids

48.41

Weight (kDa)

8.16

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 11 - 395 4.4e-66 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 680
AatII GACGTC 1 cut(s) 523
AccII CGCG 3 cut(s) 906, 974, 1249
AciI CCGC 1 cut(s) 1249
AclWI GGATC 2 cut(s) 67, 233
AcoI YGGCCR 2 cut(s) 575, 1110
AcsI RAATTY 4 cut(s) 60, 253, 278, 1019
AcuI CTGAAG 4 cut(s) 326, 334, 687, 822
AcyI GRCGYC 1 cut(s) 520
AdeI CACNNNGTG 1 cut(s) 917
AfaI GTAC 4 cut(s) 94, 198, 925, 1004
AfiI CCNNNNNNNGG 3 cut(s) 417, 558, 849
AgsI TTSAA 5 cut(s) 52, 139, 539, 941, 1024
AjnI CCWGG 2 cut(s) 481, 1085
AluBI AGCT 5 cut(s) 186, 333, 424, 640, 707
AluI AGCT 5 cut(s) 186, 333, 424, 640, 707
Alw26I GTCTC 4 cut(s) 451, 527, 609, 1148
AlwI GGATC 2 cut(s) 67, 233
AlwNI CAGNNNCTG 2 cut(s) 304, 921
AoxI GGCC 4 cut(s) 575, 1110, 1200, 1250
ApeKI GCWGC 4 cut(s) 381, 421, 640, 707
ApoI RAATTY 4 cut(s) 60, 253, 278, 1019
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 47
AspLEI GCGC 2 cut(s) 228, 1249
AspS9I GGNCC 3 cut(s) 1009, 1082, 1201
AsuC2I CCSGG 1 cut(s) 1007
AsuHPI GGTGA 2 cut(s) 38, 1130
AvaII GGWCC 2 cut(s) 1009, 1082
BalI TGGCCA 2 cut(s) 577, 1112
BanII GRGCYC 1 cut(s) 411
BarI GAAGNNNNNNTAC 2 cut(s) 579, 611
BbsI GAAGAC 1 cut(s) 546
BbvI GCAGC 4 cut(s) 368, 433, 627, 694
BccI CCATC 2 cut(s) 446, 1120
BceAI ACGGC 1 cut(s) 1260
BciT130I CCWGG 2 cut(s) 483, 1087
BciVI GTATCC 1 cut(s) 909
BcnI CCSGG 1 cut(s) 1007
BcoDI GTCTC 4 cut(s) 451, 527, 609, 1148
BfaI CTAG 6 cut(s) 33, 314, 489, 1032, 1190, 1212
BfmI CTRYAG 3 cut(s) 441, 596, 927
BfoI RGCGCY 1 cut(s) 229
BfuI GTATCC 1 cut(s) 909
BisI GCNGC 5 cut(s) 382, 422, 641, 708, 1250
BlsI GCNGC 5 cut(s) 383, 423, 642, 709, 1251
Bme1390I CCNGG 3 cut(s) 483, 1007, 1087
Bme18I GGWCC 2 cut(s) 1009, 1082
BmgT120I GGNCC 3 cut(s) 1009, 1082, 1201
BmiI GGNNCC 2 cut(s) 1011, 1084
BmrFI CCNGG 3 cut(s) 483, 1007, 1087
BmsI GCATC 3 cut(s) 361, 513, 818
BpiI GAAGAC 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 446
BpuMI CCSGG 1 cut(s) 1007
BsaHI GRCGYC 1 cut(s) 520
BsaJI CCNNGG 1 cut(s) 1085
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 417, 558, 849
Bse1I ACTGG 1 cut(s) 1185
Bse3DI GCAATG 2 cut(s) 381, 717
BseBI CCWGG 2 cut(s) 483, 1087
BseDI CCNNGG 1 cut(s) 1085
BseGI GGATG 6 cut(s) 366, 409, 457, 742, 791, 882
BseLI CCNNNNNNNGG 3 cut(s) 417, 558, 849
BseMI GCAATG 2 cut(s) 381, 717
BseMII CTCAG 1 cut(s) 928
BseNI ACTGG 1 cut(s) 1185
BseRI GAGGAG 1 cut(s) 574
BseXI GCAGC 4 cut(s) 368, 433, 627, 694
BsgI GTGCAG 1 cut(s) 421
Bsh1236I CGCG 3 cut(s) 906, 974, 1249
BshFI GGCC 4 cut(s) 577, 1112, 1202, 1252
BsiSI CCGG 1 cut(s) 1007
BslFI GGGAC 1 cut(s) 1215
BslI CCNNNNNNNGG 3 cut(s) 417, 558, 849
BsmAI GTCTC 4 cut(s) 451, 527, 609, 1148
BsmBI CGTCTC 2 cut(s) 527, 1148
BsmFI GGGAC 1 cut(s) 1215
BsnI GGCC 4 cut(s) 577, 1112, 1202, 1252
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 3 cut(s) 72, 238, 1174
BspACI CCGC 1 cut(s) 1249
BspANI GGCC 4 cut(s) 577, 1112, 1202, 1252
BspCNI CTCAG 1 cut(s) 927
BspFNI CGCG 3 cut(s) 906, 974, 1249
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 2 cut(s) 1011, 1084
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 2 cut(s) 67, 233
BsrDI GCAATG 2 cut(s) 381, 717
BsrI ACTGG 1 cut(s) 1185
BssECI CCNNGG 1 cut(s) 1085
BssMI GATC 3 cut(s) 72, 238, 1174
BssNI GRCGYC 1 cut(s) 520
Bst2UI CCWGG 2 cut(s) 483, 1087
Bst4CI ACNGT 7 cut(s) 600, 848, 871, 886, 923, 1142, 1168
Bst6I CTCTTC 1 cut(s) 607
BstACI GRCGYC 1 cut(s) 520
BstC8I GCNNGC 3 cut(s) 188, 386, 514
BstDEI CTNAG 5 cut(s) 132, 416, 764, 914, 1217
BstENI CCTNNNNNAGG 1 cut(s) 415
BstF5I GGATG 6 cut(s) 366, 409, 457, 742, 791, 882
BstFNI CGCG 3 cut(s) 906, 974, 1249
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 2 cut(s) 228, 1249
BstKTI GATC 3 cut(s) 75, 241, 1177
BstMAI GTCTC 4 cut(s) 451, 527, 609, 1148
BstMBI GATC 3 cut(s) 72, 238, 1174
BstMWI GCNNNNNNNGC 1 cut(s) 421
BstNI CCWGG 2 cut(s) 483, 1087
BstNSI RCATGY 4 cut(s) 388, 962, 981, 1095
BstSCI CCNGG 3 cut(s) 481, 1005, 1085
BstSFI CTRYAG 3 cut(s) 441, 596, 927
BstUI CGCG 3 cut(s) 906, 974, 1249
BstV1I GCAGC 4 cut(s) 368, 433, 627, 694
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 2 cut(s) 72, 238
BstYI RGATCY 2 cut(s) 72, 238
BsuI GTATCC 1 cut(s) 909
BsuRI GGCC 4 cut(s) 577, 1112, 1202, 1252
BtsCI GGATG 6 cut(s) 366, 409, 457, 742, 791, 882
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 2 cut(s) 438, 922
Cac8I GCNNGC 3 cut(s) 188, 386, 514
CaiI CAGNNNCTG 2 cut(s) 304, 921
CciI TCATGA 1 cut(s) 474
CfoI GCGC 2 cut(s) 228, 1249
Cfr13I GGNCC 3 cut(s) 1009, 1082, 1201
CseI GACGC 1 cut(s) 980
Csp6I GTAC 4 cut(s) 93, 197, 924, 1003
CviQI GTAC 4 cut(s) 93, 197, 924, 1003
DdeI CTNAG 5 cut(s) 132, 416, 764, 914, 1217
DpnI GATC 3 cut(s) 74, 240, 1176
DpnII GATC 3 cut(s) 72, 238, 1174
DraIII CACNNNGTG 1 cut(s) 917
EaeI YGGCCR 2 cut(s) 575, 1110
Eam1104I CTCTTC 1 cut(s) 607
EarI CTCTTC 1 cut(s) 607
Eco24I GRGCYC 1 cut(s) 411
Eco47I GGWCC 2 cut(s) 1009, 1082
Eco57I CTGAAG 4 cut(s) 326, 334, 687, 822
EcoNI CCTNNNNNAGG 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 1082
EcoRI GAATTC 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 481, 1085
EcoT22I ATGCAT 1 cut(s) 983
EcoT38I GRGCYC 1 cut(s) 411
Esp3I CGTCTC 2 cut(s) 527, 1148
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FaqI GGGAC 1 cut(s) 1215
Fnu4HI GCNGC 5 cut(s) 382, 422, 641, 708, 1250
FokI GGATG 6 cut(s) 373, 416, 464, 749, 778, 889
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 5 cut(s) 382, 422, 641, 708, 1250
FspBI CTAG 6 cut(s) 33, 314, 489, 1032, 1190, 1212
GlaI GCGC 2 cut(s) 227, 1248
GluI GCNGC 5 cut(s) 382, 422, 641, 708, 1250
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 4 cut(s) 577, 1112, 1202, 1252
HapII CCGG 1 cut(s) 1007
HgaI GACGC 1 cut(s) 980
HhaI GCGC 2 cut(s) 228, 1249
Hin1I GRCGYC 1 cut(s) 520
Hin6I GCGC 2 cut(s) 226, 1247
HinP1I GCGC 2 cut(s) 226, 1247
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HindIII AAGCTT 2 cut(s) 184, 331
HinfI GANTC 4 cut(s) 456, 478, 617, 1101
HpaII CCGG 1 cut(s) 1007
HphI GGTGA 2 cut(s) 38, 1130
Hpy166II GTNNAC 4 cut(s) 197, 882, 964, 1164
Hpy188I TCNGA 3 cut(s) 238, 300, 451
Hpy188III TCNNGA 7 cut(s) 52, 475, 734, 1032, 1080, 1178, 1190
Hpy8I GTNNAC 4 cut(s) 197, 882, 964, 1164
HpyAV CCTTC 8 cut(s) 549, 562, 797, 847, 926, 1023, 1038, 1192
HpyCH4III ACNGT 7 cut(s) 600, 848, 871, 886, 923, 1142, 1168
HpyCH4IV ACGT 1 cut(s) 520
HpyF10VI GCNNNNNNNGC 1 cut(s) 421
HpyF3I CTNAG 5 cut(s) 132, 416, 764, 914, 1217
HpySE526I ACGT 1 cut(s) 520
Hsp92I GRCGYC 1 cut(s) 520
HspAI GCGC 2 cut(s) 226, 1247
Kzo9I GATC 3 cut(s) 72, 238, 1174
LmnI GCTCC 1 cut(s) 806
Lsp1109I GCAGC 4 cut(s) 368, 433, 627, 694
LweI GCATC 3 cut(s) 361, 513, 818
MaeI CTAG 6 cut(s) 33, 314, 489, 1032, 1190, 1212
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 4 cut(s) 397, 606, 814, 917
MalI GATC 3 cut(s) 74, 240, 1176
MboI GATC 3 cut(s) 72, 238, 1174
MboII GAAGA 7 cut(s) 255, 319, 551, 599, 624, 836, 1127
MfeI CAATTG 1 cut(s) 776
MflI RGATCY 2 cut(s) 72, 238
MhlI GDGCHC 1 cut(s) 411
MlsI TGGCCA 2 cut(s) 577, 1112
MluCI AATT 7 cut(s) 12, 60, 253, 278, 681, 776, 1019
MluNI TGGCCA 2 cut(s) 577, 1112
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 1 cut(s) 830
MnlI CCTC 7 cut(s) 91, 351, 552, 636, 763, 830, 912
Mox20I TGGCCA 2 cut(s) 577, 1112
Mph1103I ATGCAT 1 cut(s) 983
MroXI GAANNNNTTC 1 cut(s) 47
MscI TGGCCA 2 cut(s) 577, 1112
MseI TTAA 3 cut(s) 831, 861, 1257
MslI CAYNNNNRTG 2 cut(s) 389, 1159
Msp20I TGGCCA 2 cut(s) 577, 1112
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 1 cut(s) 1007
MspR9I CCNGG 3 cut(s) 483, 1007, 1087
MunI CAATTG 1 cut(s) 776
MvaI CCWGG 2 cut(s) 483, 1087
MvnI CGCG 3 cut(s) 906, 974, 1249
MwoI GCNNNNNNNGC 1 cut(s) 421
NciI CCSGG 1 cut(s) 1007
NdeII GATC 3 cut(s) 72, 238, 1174
NlaIV GGNNCC 2 cut(s) 1011, 1084
NmuCI GTSAC 2 cut(s) 397, 917
NsiI ATGCAT 1 cut(s) 983
NspI RCATGY 4 cut(s) 388, 962, 981, 1095
PaeI GCATGC 1 cut(s) 388
PagI TCATGA 1 cut(s) 474
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 3 cut(s) 456, 478, 1101
PkrI GCNGC 5 cut(s) 383, 423, 642, 709, 1251
PleI GAGTC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 611
PpuMI RGGWCCY 1 cut(s) 1082
PshBI ATTAAT 1 cut(s) 831
PsiI TTATAA 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 1082
Psp6I CCWGG 2 cut(s) 481, 1085
PspGI CCWGG 2 cut(s) 481, 1085
PspN4I GGNNCC 2 cut(s) 1011, 1084
PspPI GGNCC 3 cut(s) 1009, 1082, 1201
PspPPI RGGWCCY 1 cut(s) 1082
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 2 cut(s) 304, 921
PsuI RGATCY 2 cut(s) 72, 238
PvuII CAGCTG 1 cut(s) 707
RsaI GTAC 4 cut(s) 94, 198, 925, 1004
RsaNI GTAC 4 cut(s) 93, 197, 924, 1003
RseI CAYNNNNRTG 2 cut(s) 389, 1159
SaqAI TTAA 3 cut(s) 831, 861, 1257
SatI GCNGC 5 cut(s) 382, 422, 641, 708, 1250
Sau3AI GATC 3 cut(s) 72, 238, 1174
Sau96I GGNCC 3 cut(s) 1009, 1082, 1201
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 3 cut(s) 483, 1007, 1087
SduI GDGCHC 1 cut(s) 411
SfaNI GCATC 3 cut(s) 361, 513, 818
SfcI CTRYAG 3 cut(s) 441, 596, 927
SinI GGWCC 2 cut(s) 1009, 1082
SmiMI CAYNNNNRTG 2 cut(s) 389, 1159
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
SphI GCATGC 1 cut(s) 388
Sse9I AATT 7 cut(s) 12, 60, 253, 278, 681, 776, 1019
SsiI CCGC 1 cut(s) 1249
SspMI CTAG 6 cut(s) 33, 314, 489, 1032, 1190, 1212
StyD4I CCNGG 3 cut(s) 481, 1005, 1085
TaaI ACNGT 7 cut(s) 600, 848, 871, 886, 923, 1142, 1168
TaiI ACGT 1 cut(s) 523
TaqI TCGA 4 cut(s) 282, 782, 822, 1170
TasI AATT 7 cut(s) 12, 60, 253, 278, 681, 776, 1019
TatI WGTACW 2 cut(s) 92, 923
TauI GCSGC 1 cut(s) 1252
TfiI GAWTC 3 cut(s) 456, 478, 1101
Tru1I TTAA 3 cut(s) 831, 861, 1257
Tru9I TTAA 3 cut(s) 831, 861, 1257
TscAI CASTG 2 cut(s) 445, 922
TseFI GTSAC 2 cut(s) 397, 917
TseI GCWGC 4 cut(s) 381, 421, 640, 707
Tsp45I GTSAC 2 cut(s) 397, 917
TspDTI ATGAA 5 cut(s) 259, 266, 648, 1054, 1056
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 2 cut(s) 445, 922
VpaK11BI GGWCC 2 cut(s) 1009, 1082
VspI ATTAAT 1 cut(s) 831
XagI CCTNNNNNAGG 1 cut(s) 415
XapI RAATTY 4 cut(s) 60, 253, 278, 1019
XbaI TCTAGA 2 cut(s) 1031, 1189
XceI RCATGY 4 cut(s) 388, 962, 981, 1095
XcmI CCANNNNNNNNNTGG 1 cut(s) 1093
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 6 cut(s) 33, 314, 489, 1032, 1190, 1212
ZraI GACGTC 1 cut(s) 521
Zsp2I ATGCAT 1 cut(s) 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.