FvH4_6g31020

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004864
Physical Location & Seq
Reverse (-)
24203709 .. 24208556
4848 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31020.t4

Sequence Viewer

Length: 912 bp
ATGGCTCTTTCGCTAACTATTGAGAAAGAATTTGCTCAAACCTCAGAAAGAGTGAAGTCTGTGGATCTGCATCCTACGGAGCCATGGATGCTAGCAAGTTTGTATTCAGGAACTATTTGCATCTGGAACTACCAGTCACAGACCCAGGAGAAGTCCATCAAGGTCACTGAGTCACCAGTCAGATCAGTAAAGTTCGTGGCTCGCAAACACTGGATTGTAACCGGTGCTGATGACAAGTTCATTCGCATATACAACTACGATACTATGGAAAAGATCAAAGAGTTTGAAGCACACACTGACTTTATCAGGAGTGTCACTATCCATCCAACTCTACCATATCTGCTGTCATGTTCTGACGACAAGGTCATTAAGCTTTGGGACTGGGAGAAGAACTGGGAATGCACACAAGTCTTTGAGGGACATTCTCACTATGTGATGCAAGTGGCTTTCAATCCAAAAGACACCAACAGTTTTGCTAGTGCCTCTCTTGATGGAACCATTAAGATATGGAACATTGGCTCTCCTGCCCCTAATGTTACCTTAGAAGGCCACTTAAAAGGAGTTAATTGTATTGATTACTTCATTCGTGGAGATAAGACATACCTATTGAGTGGTTCTGATGACTTCACTGCTAAGGTATGGGACTATGAAACTACAAGTTGTGTGAAAACTCTAGAAGGACATGAGCACAATGTAACAGCTGTTAGTGTTCATCCTGAGCTTCCCATAATCATTACAACTTCTGAGGATGGGACTATTCAAATATGGAATGCAGCCACATTTAGTCTAGAGCACAAACTGAATTATGCTCTTGAAAGAGTTTGGGCCATGGGACACTTGAAAGGATCATGCAAGGTTGCATTTGGTTTTGACAATGGAACCATCATTGTCCAAATGAATGCTGCTGCTTGA

Protein Analysis

304

Amino Acids

34.32

Weight (kDa)

5.73

Isoelectric Point (pI)

27.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 4 - 171 8.6e-20 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 10 - 174 8.5e-13 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 155 5e-20 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 10 - 114 3.5e-12 THOC3 beta-propeller domain
EIF3I PF24805 13 - 111 1.2e-06 EIF3I
WDR55 PF24796 14 - 199 2.6e-19 WDR55
WD40_Prp19 PF24814 14 - 192 7.2e-26 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 20 - 172 2e-19 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 35 - 208 5.8e-15 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 35 - 193 3.8e-20 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_1st PF24780 38 - 170 7.1e-06 MABP1/WDR62 first WD40 domain
Beta-prop_WDR5 PF25175 55 - 172 1.4e-27 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 58 - 131 2.1e-11 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 64 - 172 7.7e-11 MABP1/WDR62 second WD40 domain
WD40 PF00400 90 - 127 4.1e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 98 - 172 7.5e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 114 - 189 3e-15 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 114 - 170 3.1e-06 EIPR1 beta-propeller
WD40 PF00400 132 - 171 5e-09 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 133 - 278 4.5e-28 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 134 - 259 4.7e-11 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 135 - 182 4e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 145 - 261 1.1e-19 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 153 - 277 1.7e-14 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 154 - 274 3.1e-27 WDR5 beta-propeller domain
WD40_Gbeta PF25391 154 - 257 3e-11 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 164 - 266 3.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 164 - 256 7.1e-06 WDR36/Utp21 first beta-propeller
EIF3I PF24805 179 - 260 5.4e-07 EIF3I
WD40 PF00400 179 - 215 2.8e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 180 - 268 2.5e-14 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 180 - 267 4.1e-08 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 195 - 268 2.9e-14 WDHD1 first WD40 domain
WDR55 PF24796 196 - 270 2.2e-08 WDR55
Beta-prop_WDR75_1st PF23869 197 - 257 2.3e-07 WD repeat-containing protein 75 first beta-propeller
WD40_CDC20-Fz PF24807 197 - 267 2.4e-07 CDC20/Fizzy WD40 domain
Beta-prop_SCAP PF24017 202 - 262 9.1e-06 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 206 - 260 2.4e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 220 - 257 2e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
AclWI GGATC 2 cut(s) 72, 853
AcsI RAATTY 1 cut(s) 29
AdeI CACNNNGTG 1 cut(s) 433
AgeI ACCGGT 1 cut(s) 221
AgsI TTSAA 5 cut(s) 287, 451, 761, 815, 841
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 3 cut(s) 373, 701, 721
AluI AGCT 3 cut(s) 373, 701, 721
Alw21I GWGCWC 2 cut(s) 690, 795
AlwI GGATC 2 cut(s) 72, 853
AoxI GGCC 2 cut(s) 547, 825
ApeKI GCWGC 3 cut(s) 773, 902, 905
ApoI RAATTY 1 cut(s) 29
AsiGI ACCGGT 1 cut(s) 221
AspS9I GGNCC 1 cut(s) 825
AsuHPI GGTGA 1 cut(s) 165
AsuNHI GCTAGC 1 cut(s) 91
Bbv12I GWGCWC 2 cut(s) 690, 795
BbvI GCAGC 3 cut(s) 785, 889, 892
BccI CCATC 5 cut(s) 164, 330, 485, 743, 890
BciT130I CCWGG 1 cut(s) 146
BfaI CTAG 4 cut(s) 92, 477, 674, 788
BisI GCNGC 3 cut(s) 774, 903, 906
BlsI GCNGC 3 cut(s) 775, 904, 907
Bme1390I CCNGG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 825
BmiI GGNNCC 3 cut(s) 81, 496, 880
BmrFI CCNGG 1 cut(s) 146
BmrI ACTGGG 2 cut(s) 391, 403
BmsI GCATC 4 cut(s) 78, 79, 129, 426
BmtI GCTAGC 1 cut(s) 95
BmuI ACTGGG 2 cut(s) 391, 403
Bpu10I CCTNAGC 2 cut(s) 633, 717
BsaBI GATNNNNATC 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 83, 144, 828
BsaWI WCCGGW 1 cut(s) 221
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 5 cut(s) 133, 176, 215, 386, 398
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 3 cut(s) 83, 144, 828
BseGI GGATG 5 cut(s) 70, 93, 322, 712, 754
BseJI GATNNNNATC 1 cut(s) 69
BseMII CTCAG 4 cut(s) 57, 159, 708, 735
BseNI ACTGG 5 cut(s) 133, 176, 215, 386, 398
BseXI GCAGC 3 cut(s) 785, 889, 892
BshFI GGCC 2 cut(s) 549, 827
BshTI ACCGGT 1 cut(s) 221
BsiHKAI GWGCWC 2 cut(s) 690, 795
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 5 cut(s) 392, 432, 656, 766, 846
BsmFI GGGAC 5 cut(s) 392, 432, 656, 766, 846
BsmI GAATGC 3 cut(s) 404, 775, 904
BsnI GGCC 2 cut(s) 549, 827
Bsp1286I GDGCHC 2 cut(s) 690, 795
Bsp143I GATC 4 cut(s) 64, 182, 273, 845
Bsp19I CCATGG 2 cut(s) 83, 828
BspANI GGCC 2 cut(s) 549, 827
BspCNI CTCAG 4 cut(s) 56, 160, 709, 736
BspLI GGNNCC 3 cut(s) 81, 496, 880
BspOI GCTAGC 1 cut(s) 95
BspPI GGATC 2 cut(s) 72, 853
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 5 cut(s) 133, 176, 215, 386, 398
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 3 cut(s) 83, 144, 828
BssMI GATC 4 cut(s) 64, 182, 273, 845
BssT1I CCWWGG 2 cut(s) 83, 828
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 93, 202
BstDEI CTNAG 6 cut(s) 43, 168, 541, 633, 717, 744
BstDSI CCRYGG 2 cut(s) 83, 828
BstF5I GGATG 5 cut(s) 70, 93, 322, 712, 754
BstKTI GATC 4 cut(s) 67, 185, 276, 848
BstMBI GATC 4 cut(s) 64, 182, 273, 845
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 785, 889, 892
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BsuRI GGCC 2 cut(s) 549, 827
BtgI CCRYGG 2 cut(s) 83, 828
BtsCI GGATG 5 cut(s) 70, 93, 322, 712, 754
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 4 cut(s) 165, 208, 294, 627
Cac8I GCNNGC 2 cut(s) 93, 202
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 825
CspAI ACCGGT 1 cut(s) 221
CviAII CATG 5 cut(s) 84, 348, 683, 829, 849
DdeI CTNAG 6 cut(s) 43, 168, 541, 633, 717, 744
DpnI GATC 4 cut(s) 66, 184, 275, 847
DpnII GATC 4 cut(s) 64, 182, 273, 845
DraIII CACNNNGTG 1 cut(s) 433
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
Eco130I CCWWGG 2 cut(s) 83, 828
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 83, 828
ErhI CCWWGG 2 cut(s) 83, 828
FaeI CATG 5 cut(s) 87, 351, 686, 832, 852
FaqI GGGAC 5 cut(s) 392, 432, 656, 766, 846
FatI CATG 5 cut(s) 83, 347, 682, 828, 848
Fnu4HI GCNGC 3 cut(s) 774, 903, 906
FokI GGATG 5 cut(s) 57, 100, 309, 699, 761
Fsp4HI GCNGC 3 cut(s) 774, 903, 906
FspBI CTAG 4 cut(s) 92, 477, 674, 788
GluI GCNGC 3 cut(s) 774, 903, 906
HaeIII GGCC 2 cut(s) 549, 827
HapII CCGG 1 cut(s) 222
Hin1II CATG 5 cut(s) 87, 351, 686, 832, 852
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 1 cut(s) 170
HpaII CCGG 1 cut(s) 222
HphI GGTGA 1 cut(s) 165
Hpy188I TCNGA 5 cut(s) 46, 182, 355, 619, 745
Hpy188III TCNNGA 8 cut(s) 108, 124, 307, 488, 674, 716, 788, 812
HpyAV CCTTC 2 cut(s) 539, 671
HpyCH4III ACNGT 1 cut(s) 470
HpyCH4V TGCA 7 cut(s) 70, 120, 402, 439, 773, 852, 860
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 6 cut(s) 43, 168, 541, 633, 717, 744
Hsp92II CATG 5 cut(s) 87, 351, 686, 832, 852
Kzo9I GATC 4 cut(s) 64, 182, 273, 845
LmnI GCTCC 1 cut(s) 79
Lsp1109I GCAGC 3 cut(s) 785, 889, 892
LweI GCATC 4 cut(s) 78, 79, 129, 426
MaeI CTAG 4 cut(s) 92, 477, 674, 788
MaeIII GTNAC 7 cut(s) 135, 163, 171, 217, 313, 535, 694
MalI GATC 4 cut(s) 66, 184, 275, 847
MboI GATC 4 cut(s) 64, 182, 273, 845
MboII GAAGA 1 cut(s) 400
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 2 cut(s) 690, 795
MluCI AATT 3 cut(s) 29, 565, 802
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 350
MnlI CCTC 4 cut(s) 52, 409, 493, 739
MseI TTAA 4 cut(s) 369, 501, 554, 564
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 146
Mva1269I GAATGC 3 cut(s) 404, 775, 904
MvaI CCWGG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 88
NcoI CCATGG 2 cut(s) 83, 828
NdeII GATC 4 cut(s) 64, 182, 273, 845
NheI GCTAGC 1 cut(s) 91
NlaIII CATG 5 cut(s) 87, 351, 686, 832, 852
NlaIV GGNNCC 3 cut(s) 81, 496, 880
NmuCI GTSAC 4 cut(s) 135, 163, 171, 313
PctI GAATGC 3 cut(s) 404, 775, 904
PflFI GACNNNGTC 1 cut(s) 362
PinAI ACCGGT 1 cut(s) 221
PkrI GCNGC 3 cut(s) 775, 904, 907
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 3 cut(s) 81, 496, 880
PspPI GGNCC 1 cut(s) 825
PsuI RGATCY 1 cut(s) 64
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 701
SaqAI TTAA 4 cut(s) 369, 501, 554, 564
SatI GCNGC 3 cut(s) 774, 903, 906
Sau3AI GATC 4 cut(s) 64, 182, 273, 845
Sau96I GGNCC 1 cut(s) 825
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 2 cut(s) 690, 795
SfaNI GCATC 4 cut(s) 78, 79, 129, 426
Sse9I AATT 3 cut(s) 29, 565, 802
SspMI CTAG 4 cut(s) 92, 477, 674, 788
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 2 cut(s) 83, 828
TaaI ACNGT 1 cut(s) 470
TasI AATT 3 cut(s) 29, 565, 802
Tru1I TTAA 4 cut(s) 369, 501, 554, 564
Tru9I TTAA 4 cut(s) 369, 501, 554, 564
TscAI CASTG 4 cut(s) 172, 215, 301, 634
TseFI GTSAC 4 cut(s) 135, 163, 171, 313
TseI GCWGC 3 cut(s) 773, 902, 905
Tsp45I GTSAC 4 cut(s) 135, 163, 171, 313
TspDTI ATGAA 5 cut(s) 229, 571, 663, 701, 911
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 4 cut(s) 172, 215, 301, 634
Tth111I GACNNNGTC 1 cut(s) 362
XapI RAATTY 1 cut(s) 29
XbaI TCTAGA 2 cut(s) 673, 787
XspI CTAG 4 cut(s) 92, 477, 674, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.