MD06G1184300.v1.1

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004864
Physical Location & Seq
Forward (+)
32256975 .. 32259596
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1184300.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGGATTAGGATATGATAATTTTCCTTGGCTGCCAAGCCTCACTGTGTATATAAATGGAGGTTCTGCATCGTCCTCCATTCACAACCACTTTTACACGTACATCAAGTTAACAATTTTCTTCATATTTCCTTCCTTCTATTTGCTCAAAATGGCTCTTTCACTCACTATTGAGAAAGAATTTGCTCAAAACTCAGAAAGAATTAAGTCTGTGGATCTACATCCAACTGAGCCATGGGTTCTGGTCAGTTTGTATTCAGGTGCTGTTTGTATCTGGAATTACCAATCACAGACCATGGAGAAGTCCTTCAAGGTCACTGAATCACCAGTCAGGTCAGCAAAGTTTGTAGCACGCGAACACTGGATAGTAACCGCAGCTGATGACACATTTATTCGCGTCTACAACTATGACACGACGGAAAAGATCAAAGAGTTTGAAGCACATACAGATTATATCAGGAGTGTGACTGTCCATCCTACTCTACCTTATCTCTTGTCATGCTCGGACGACAACGTTATAAAGCTTTGGGATTGGGAGAAGGATTGGTCATGCACTCAGATCTTTGAGGGACATTCTCACTATGTAATGCAAGTGGCGTTCAATCCAAACGACACCAATACTTTCTCTAGTGCGTCTCTTGATGGCACTGTCAAGGTATGGAGGATTGGCTCCGCTGGCCCAGAGTTTACATTAGAGGACCACTTAAAAGGAGTAAATTGCGTTCATTACTTCATTCGCGACAATAAAACATATCTATTGAGTGGTTCCGACGATTTTACTGTTAAGGTATGGGACTACGAAATCAAAAGCTGTGTCCAAACTTTAGAAGGCCATGAGCACAATGTAACTGCTCTATGTGTTCATCCTGAGCTTCCCATAATAATCACAGCTTCTGAGGATGGGAATGTTCACATTTGGCATGCAGCCACTTTTAGGCTAGAAAAGAGACTGAGCTACGATCTCGAAAGAGTTTGGGCCATCGCACACTCGAAAGGATCAGACAAAGTTGCATTTGGATTTGACAAAGGAATAGTTGTGGTTAAAATGAATGGTCATGTTGATCTCCAAGCTTAG

Protein Analysis

358

Amino Acids

40.85

Weight (kDa)

5.76

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 53 - 221 4.1e-17 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 56 - 164 1.1e-11 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 59 - 224 1.7e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 60 - 176 5.9e-09 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 60 - 222 9.2e-24 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 63 - 221 4.5e-27 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 63 - 155 2.4e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML PF23409 65 - 272 2.1e-11 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 65 - 181 4e-17 Prp19 WD40 domain
WDR55 PF24796 66 - 249 1.3e-17 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 80 - 220 1.1e-08 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 87 - 336 1.2e-25 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 108 - 181 7e-11 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 117 - 222 2.3e-09 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 119 - 306 1e-19 G protein beta WD-40 repeat protein
Beta-prop_WDR75_1st PF23869 126 - 179 3.3e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 141 - 177 5.4e-09 WD domain, G-beta repeat
EIF3I PF24805 145 - 318 1.3e-09 EIF3I
Beta-prop_THOC3 PF25174 164 - 239 9.3e-13 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 164 - 220 8.6e-06 EIPR1 beta-propeller
Beta-prop_EML PF23409 167 - 333 1.6e-10 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 182 - 220 2.4e-08 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 183 - 225 3.2e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 184 - 333 3.2e-25 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 185 - 241 4.2e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 204 - 332 1.8e-25 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 206 - 345 1.1e-11 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 207 - 310 4.5e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 211 - 310 4e-06 SCAP Beta-propeller
WD40 PF00400 228 - 265 5e-07 WD domain, G-beta repeat
WDR55 PF24796 233 - 346 4.7e-10 WDR55
WD40_Prp19 PF24814 236 - 311 1.6e-12 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 244 - 340 7.7e-13 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 247 - 310 9.7e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_THOC3 PF25174 248 - 343 1.3e-13 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 249 - 309 3.6e-06 TEP-1 second beta-propeller
WD40 PF00400 270 - 306 3.9e-09 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 283 - 347 8.9e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
AccI GTMKAC 1 cut(s) 399
AccII CGCG 3 cut(s) 354, 396, 738
AciI CCGC 2 cut(s) 372, 672
AclI AACGTT 1 cut(s) 513
AclWI GGATC 2 cut(s) 222, 1003
AcsI RAATTY 1 cut(s) 179
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 933
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 3 cut(s) 310, 437, 601
AluBI AGCT 7 cut(s) 377, 523, 810, 871, 890, 954, 1070
AluI AGCT 7 cut(s) 377, 523, 810, 871, 890, 954, 1070
Alw21I GWGCWC 1 cut(s) 840
Alw26I GTCTC 2 cut(s) 639, 940
AlwI GGATC 2 cut(s) 222, 1003
AlwNI CAGNNNCTG 2 cut(s) 263, 893
AoxI GGCC 3 cut(s) 676, 829, 975
ApeKI GCWGC 3 cut(s) 31, 374, 923
ApoI RAATTY 1 cut(s) 179
ArsI GACNNNNNNTTYG 2 cut(s) 798, 830
Asp700I GAANNNNTTC 1 cut(s) 305
AspS9I GGNCC 3 cut(s) 677, 697, 975
AsuHPI GGTGA 1 cut(s) 315
AvaII GGWCC 1 cut(s) 697
Bbv12I GWGCWC 1 cut(s) 840
BbvI GCAGC 3 cut(s) 18, 386, 935
BccI CCATC 4 cut(s) 480, 635, 893, 986
BcoDI GTCTC 2 cut(s) 639, 940
BfaI CTAG 2 cut(s) 627, 938
BglII AGATCT 1 cut(s) 558
BisI GCNGC 3 cut(s) 32, 375, 924
BlsI GCNGC 3 cut(s) 33, 376, 925
Bme18I GGWCC 1 cut(s) 697
BmgT120I GGNCC 3 cut(s) 677, 697, 975
BmiI GGNNCC 2 cut(s) 670, 766
BmsI GCATC 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 867
BsaAI YACGTR 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 26, 233, 294
Bsc4I CCNNNNNNNGG 1 cut(s) 933
Bse1I ACTGG 2 cut(s) 326, 365
Bse8I GATNNNNATC 1 cut(s) 219
BseDI CCNNGG 3 cut(s) 26, 233, 294
BseGI GGATG 4 cut(s) 220, 472, 862, 904
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 933
BseMII CTCAG 6 cut(s) 207, 219, 569, 858, 885, 941
BseNI ACTGG 2 cut(s) 326, 365
BseXI GCAGC 3 cut(s) 18, 386, 935
Bsh1236I CGCG 3 cut(s) 354, 396, 738
BshFI GGCC 3 cut(s) 678, 831, 977
BsiHKAI GWGCWC 1 cut(s) 840
BslFI GGGAC 2 cut(s) 582, 806
BslI CCNNNNNNNGG 1 cut(s) 933
BsmAI GTCTC 2 cut(s) 639, 940
BsmBI CGTCTC 1 cut(s) 639
BsmFI GGGAC 2 cut(s) 582, 806
BsnI GGCC 3 cut(s) 678, 831, 977
Bsp1286I GDGCHC 1 cut(s) 840
Bsp143I GATC 6 cut(s) 214, 423, 558, 958, 995, 1060
Bsp19I CCATGG 2 cut(s) 233, 294
Bsp68I TCGCGA 1 cut(s) 738
BspACI CCGC 2 cut(s) 372, 672
BspANI GGCC 3 cut(s) 678, 831, 977
BspCNI CTCAG 6 cut(s) 206, 220, 568, 859, 886, 942
BspFNI CGCG 3 cut(s) 354, 396, 738
BspLI GGNNCC 2 cut(s) 670, 766
BspPI GGATC 2 cut(s) 222, 1003
BsrI ACTGG 2 cut(s) 326, 365
BssECI CCNNGG 3 cut(s) 26, 233, 294
BssMI GATC 6 cut(s) 214, 423, 558, 958, 995, 1060
BssT1I CCWWGG 3 cut(s) 26, 233, 294
Bst4CI ACNGT 4 cut(s) 46, 469, 649, 781
BstBAI YACGTR 1 cut(s) 99
BstC8I GCNNGC 3 cut(s) 352, 676, 921
BstDEI CTNAG 7 cut(s) 193, 228, 555, 867, 894, 950, 1071
BstDSI CCRYGG 2 cut(s) 233, 294
BstF5I GGATG 4 cut(s) 220, 472, 862, 904
BstFNI CGCG 3 cut(s) 354, 396, 738
BstKTI GATC 6 cut(s) 217, 426, 561, 961, 998, 1063
BstMAI GTCTC 2 cut(s) 639, 940
BstMBI GATC 6 cut(s) 214, 423, 558, 958, 995, 1060
BstMWI GCNNNNNNNGC 1 cut(s) 675
BstNSI RCATGY 1 cut(s) 923
BstUI CGCG 3 cut(s) 354, 396, 738
BstV1I GCAGC 3 cut(s) 18, 386, 935
BstX2I RGATCY 2 cut(s) 214, 558
BstYI RGATCY 2 cut(s) 214, 558
BsuRI GGCC 3 cut(s) 678, 831, 977
BtgI CCRYGG 2 cut(s) 233, 294
BtgZI GCGATG 1 cut(s) 964
BtsCI GGATG 4 cut(s) 220, 472, 862, 904
BtsIMutI CAGTG 4 cut(s) 42, 315, 358, 645
BtuMI TCGCGA 1 cut(s) 738
Cac8I GCNNGC 3 cut(s) 352, 676, 921
CaiI CAGNNNCTG 2 cut(s) 263, 893
Cfr13I GGNCC 3 cut(s) 677, 697, 975
CseI GACGC 2 cut(s) 385, 621
Csp6I GTAC 1 cut(s) 100
CviAII CATG 7 cut(s) 234, 295, 498, 549, 833, 920, 1055
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 7 cut(s) 193, 228, 555, 867, 894, 950, 1071
DpnI GATC 6 cut(s) 216, 425, 560, 960, 997, 1062
DpnII GATC 6 cut(s) 214, 423, 558, 958, 995, 1060
Eco130I CCWWGG 3 cut(s) 26, 233, 294
Eco47I GGWCC 1 cut(s) 697
EcoT14I CCWWGG 3 cut(s) 26, 233, 294
ErhI CCWWGG 3 cut(s) 26, 233, 294
Esp3I CGTCTC 1 cut(s) 639
FaeI CATG 7 cut(s) 237, 298, 501, 552, 836, 923, 1058
FaqI GGGAC 2 cut(s) 582, 806
FatI CATG 7 cut(s) 233, 294, 497, 548, 832, 919, 1054
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 32, 375, 924
FokI GGATG 4 cut(s) 207, 459, 849, 911
Fsp4HI GCNGC 3 cut(s) 32, 375, 924
FspBI CTAG 2 cut(s) 627, 938
GluI GCNGC 3 cut(s) 32, 375, 924
HaeIII GGCC 3 cut(s) 678, 831, 977
HgaI GACGC 2 cut(s) 385, 621
Hin1II CATG 7 cut(s) 237, 298, 501, 552, 836, 923, 1058
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 2 cut(s) 521, 1068
HinfI GANTC 1 cut(s) 320
HpaI GTTAAC 1 cut(s) 111
HphI GGTGA 1 cut(s) 315
Hpy166II GTNNAC 4 cut(s) 111, 400, 687, 910
Hpy188I TCNGA 6 cut(s) 196, 505, 558, 769, 895, 1000
Hpy188III TCNNGA 6 cut(s) 274, 457, 638, 737, 866, 962
Hpy8I GTNNAC 4 cut(s) 111, 400, 687, 910
Hpy99I CGWCG 2 cut(s) 418, 773
HpyAV CCTTC 5 cut(s) 141, 145, 316, 532, 821
HpyCH4III ACNGT 4 cut(s) 46, 469, 649, 781
HpyCH4IV ACGT 2 cut(s) 98, 513
HpyCH4V TGCA 5 cut(s) 68, 552, 589, 923, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 675
HpyF3I CTNAG 7 cut(s) 193, 228, 555, 867, 894, 950, 1071
HpySE526I ACGT 2 cut(s) 98, 513
Hsp92II CATG 7 cut(s) 237, 298, 501, 552, 836, 923, 1058
KspAI GTTAAC 1 cut(s) 111
Kzo9I GATC 6 cut(s) 214, 423, 558, 958, 995, 1060
LmnI GCTCC 1 cut(s) 674
Lsp1109I GCAGC 3 cut(s) 18, 386, 935
LweI GCATC 1 cut(s) 77
MaeI CTAG 2 cut(s) 627, 938
MaeII ACGT 2 cut(s) 98, 513
MaeIII GTNAC 4 cut(s) 313, 367, 463, 844
MalI GATC 6 cut(s) 216, 425, 560, 960, 997, 1062
MboI GATC 6 cut(s) 214, 423, 558, 958, 995, 1060
MboII GAAGA 1 cut(s) 112
MflI RGATCY 2 cut(s) 214, 558
MhlI GDGCHC 1 cut(s) 840
MluCI AATT 6 cut(s) 19, 114, 179, 201, 277, 715
MmeI TCCRAC 2 cut(s) 248, 792
MnlI CCTC 7 cut(s) 50, 53, 85, 559, 654, 688, 889
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 5 cut(s) 110, 204, 704, 783, 1041
MspA1I CMGCKG 2 cut(s) 377, 674
MvnI CGCG 3 cut(s) 354, 396, 738
MwoI GCNNNNNNNGC 1 cut(s) 675
NcoI CCATGG 2 cut(s) 233, 294
NdeII GATC 6 cut(s) 214, 423, 558, 958, 995, 1060
NlaIII CATG 7 cut(s) 237, 298, 501, 552, 836, 923, 1058
NlaIV GGNNCC 2 cut(s) 670, 766
NmuCI GTSAC 2 cut(s) 313, 463
NruI TCGCGA 1 cut(s) 738
NspI RCATGY 1 cut(s) 923
PaeI GCATGC 1 cut(s) 923
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 320
PkrI GCNGC 3 cut(s) 33, 376, 925
Ppu21I YACGTR 1 cut(s) 99
PsiI TTATAA 1 cut(s) 518
Psp1406I AACGTT 1 cut(s) 513
PspN4I GGNNCC 2 cut(s) 670, 766
PspPI GGNCC 3 cut(s) 677, 697, 975
PsrI GAACNNNNNNTAC 2 cut(s) 705, 737
PstNI CAGNNNCTG 2 cut(s) 263, 893
PsuI RGATCY 2 cut(s) 214, 558
PvuII CAGCTG 1 cut(s) 377
RruI TCGCGA 1 cut(s) 738
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 5 cut(s) 110, 204, 704, 783, 1041
SatI GCNGC 3 cut(s) 32, 375, 924
Sau3AI GATC 6 cut(s) 214, 423, 558, 958, 995, 1060
Sau96I GGNCC 3 cut(s) 677, 697, 975
SduI GDGCHC 1 cut(s) 840
SfaNI GCATC 1 cut(s) 77
SinI GGWCC 1 cut(s) 697
SphI GCATGC 1 cut(s) 923
Sse9I AATT 6 cut(s) 19, 114, 179, 201, 277, 715
SsiI CCGC 2 cut(s) 372, 672
SspMI CTAG 2 cut(s) 627, 938
StyI CCWWGG 3 cut(s) 26, 233, 294
TaaI ACNGT 4 cut(s) 46, 469, 649, 781
TaiI ACGT 2 cut(s) 101, 516
TaqI TCGA 2 cut(s) 963, 989
TasI AATT 6 cut(s) 19, 114, 179, 201, 277, 715
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 5 cut(s) 110, 204, 704, 783, 1041
Tru9I TTAA 5 cut(s) 110, 204, 704, 783, 1041
TscAI CASTG 4 cut(s) 49, 322, 365, 652
TseFI GTSAC 2 cut(s) 313, 463
TseI GCWGC 3 cut(s) 31, 374, 923
Tsp45I GTSAC 2 cut(s) 313, 463
TspDTI ATGAA 5 cut(s) 112, 713, 721, 851, 1061
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 4 cut(s) 49, 322, 365, 652
VpaK11BI GGWCC 1 cut(s) 697
XapI RAATTY 1 cut(s) 179
XceI RCATGY 1 cut(s) 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 2 cut(s) 627, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.