Rh2DG414400

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004864
Physical Location & Seq
Reverse (-)
60196919 .. 60216020
19102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG414400.1

Sequence Viewer

Length: 825 bp
ATGCTAGCAAGTTTGTATTCAGGAACTATTTGTATTTGGAACTACCAGTCACAGACCCTGGAGAAGTCCATCAAAGTCACTGAGTCACCAGTCAGATCAGTAAAGTTTGTAGCTCGCAAACACTGGATTATAACTGGGGCTGATGACAAGTTCATTCGGGTATACAACTACAATACCATGGAAAAGATCAAAGAGTATGAAGCACATACTGATTATATCAGGAGTGTCACTGTCCATCCAACTCTACCATATCTGCTGTCATGTTCTGATGACAAGGTTATAAAGCTTTGGGACTGGGAGAAGGACTGGGAATGCAGTCAGGTCTTTGAGGGGCATTTCCACTATGTGATGCAAGTGGCTTTCAATCCGAAAGACACCAATAGTTTTGCTAGTGCGTCTCTCGATGGAACCATTAAGACATGGAACATTGGCTCTTCTGCCCCAAATTTTACACTACAAGGCCACTCAAAAGGAGTGAATTGTGTTGATTACTTCATTCACAGCGATAAGACTTACCTGTTAAGTGGTTCTGATGACTTCACTGCCAAGGTATGGGACTATGAAACTACAAGTTGTGTGCAAACTCTAGAAGGTCATGAGAACAATGTAACAACTGTGAGTGTACATCCTGAGCTTCCCATAATAATTACAACTTCTGAGGATGGGACTGTTCATATATGGAATGCAACCACATTTAGTCTAGAGCACAAACTGAACTATGGTCTTGAAAGAGTTTGGGCCATTGGACACATGAAAGGATCATACAAGGTTGCATTTGGTTTTGACAATGGAACCACTATAGTCAAAATGAATGCTTCTGCTTGA

Protein Analysis

274

Amino Acids

31.12

Weight (kDa)

5.92

Isoelectric Point (pI)

23.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 2 - 263 1.1e-26 WDR3 second beta-propeller domain
WDR55 PF24796 3 - 170 1.3e-14 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 6 - 146 2.8e-12 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 7 - 167 4.4e-20 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 7 - 102 2.4e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 7 - 144 1e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 7 - 125 1.2e-16 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 7 - 166 2.1e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 7 - 124 1.3e-20 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 8 - 85 3e-09 THOC3 beta-propeller domain
WD40_Prp19 PF24814 8 - 106 9.5e-16 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 36 - 142 1.1e-08 MABP1/WDR62 second WD40 domain
Beta-prop_WDR75_1st PF23869 47 - 100 5.2e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 61 - 98 6.4e-09 WD domain, G-beta repeat
Beta-prop_EIPR1 PF23609 65 - 141 3.9e-06 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 69 - 161 5e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 84 - 143 4.6e-14 WDR5 beta-propeller domain
WD40 PF00400 103 - 142 1.5e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 104 - 257 7.6e-28 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 106 - 155 1.5e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 108 - 239 2.5e-21 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 125 - 249 2.9e-27 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 129 - 228 1.3e-07 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 132 - 231 3.8e-08 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 134 - 227 7.5e-06 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 137 - 237 6.5e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 148 - 186 4.8e-10 WD domain, G-beta repeat
EIF3I PF24805 151 - 231 9e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 151 - 237 3.9e-08 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 154 - 266 1.8e-11 WDR55
Beta-prop_THOC3 PF25174 155 - 241 2.9e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 160 - 263 4.2e-15 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 165 - 231 3.5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR75_1st PF23869 166 - 230 6.2e-08 WD repeat-containing protein 75 first beta-propeller
WD40_Gbeta PF25391 168 - 228 3.8e-07 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 168 - 239 7.7e-07 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 169 - 231 2e-07 TEP-1 second beta-propeller
WD40 PF00400 191 - 228 7.9e-10 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 205 - 268 8.1e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 131, 281
AccB7I CCANNNNNTGG 1 cut(s) 552
AccI GTMKAC 1 cut(s) 162
AclWI GGATC 1 cut(s) 766
AcsI RAATTY 1 cut(s) 445
AdeI CACNNNGTG 1 cut(s) 346
AfaI GTAC 1 cut(s) 624
AfiI CCNNNNNNNGG 1 cut(s) 552
AgsI TTSAA 2 cut(s) 364, 728
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 3 cut(s) 113, 286, 634
AluI AGCT 3 cut(s) 113, 286, 634
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 1 cut(s) 766
AlwNI CAGNNNCTG 1 cut(s) 58
AoxI GGCC 2 cut(s) 460, 738
ApoI RAATTY 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 738
AsuHPI GGTGA 1 cut(s) 78
AsuNHI GCTAGC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 708
BccI CCATC 4 cut(s) 77, 243, 398, 656
BciT130I CCWGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 4 cut(s) 5, 390, 587, 701
BfmI CTRYAG 1 cut(s) 798
Bme1390I CCNGG 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 738
BmiI GGNNCC 2 cut(s) 409, 793
BmrFI CCNGG 1 cut(s) 59
BmrI ACTGGG 3 cut(s) 144, 304, 316
BmsI GCATC 1 cut(s) 339
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 3 cut(s) 144, 304, 316
BpmI CTGGAG 1 cut(s) 80
Bpu10I CCTNAGC 1 cut(s) 630
BsaJI CCNNGG 3 cut(s) 57, 177, 546
Bsc4I CCNNNNNNNGG 1 cut(s) 552
Bse1I ACTGG 6 cut(s) 46, 89, 128, 139, 299, 311
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 3 cut(s) 57, 177, 546
BseGI GGATG 3 cut(s) 235, 625, 667
BseLI CCNNNNNNNGG 1 cut(s) 552
BseMII CTCAG 3 cut(s) 72, 621, 648
BseNI ACTGG 6 cut(s) 46, 89, 128, 139, 299, 311
BshFI GGCC 2 cut(s) 462, 740
BsiHKAI GWGCWC 1 cut(s) 708
BslFI GGGAC 3 cut(s) 305, 569, 679
BslI CCNNNNNNNGG 1 cut(s) 552
BsmAI GTCTC 1 cut(s) 402
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 3 cut(s) 305, 569, 679
BsmI GAATGC 3 cut(s) 317, 688, 817
BsnI GGCC 2 cut(s) 462, 740
Bsp1286I GDGCHC 1 cut(s) 708
Bsp1407I TGTACA 1 cut(s) 622
Bsp143I GATC 3 cut(s) 95, 186, 758
Bsp19I CCATGG 1 cut(s) 177
BspANI GGCC 2 cut(s) 462, 740
BspCNI CTCAG 3 cut(s) 73, 622, 649
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 409, 793
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 766
BspQI GCTCTTC 1 cut(s) 439
BsrGI TGTACA 1 cut(s) 622
BsrI ACTGG 6 cut(s) 46, 89, 128, 139, 299, 311
BssECI CCNNGG 3 cut(s) 57, 177, 546
BssMI GATC 3 cut(s) 95, 186, 758
BssNAI GTATAC 1 cut(s) 163
BssT1I CCWWGG 2 cut(s) 177, 546
Bst1107I GTATAC 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 3 cut(s) 232, 616, 670
Bst6I CTCTTC 1 cut(s) 439
BstAUI TGTACA 1 cut(s) 622
BstC8I GCNNGC 2 cut(s) 6, 115
BstDEI CTNAG 3 cut(s) 81, 630, 657
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 3 cut(s) 235, 625, 667
BstKTI GATC 3 cut(s) 98, 189, 761
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 3 cut(s) 95, 186, 758
BstNI CCWGG 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 57
BstSFI CTRYAG 1 cut(s) 798
BstZ17I GTATAC 1 cut(s) 163
BsuRI GGCC 2 cut(s) 462, 740
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 235, 625, 667
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 4 cut(s) 78, 121, 228, 540
Cac8I GCNNGC 2 cut(s) 6, 115
CaiI CAGNNNCTG 1 cut(s) 58
CciI TCATGA 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 738
CseI GACGC 1 cut(s) 384
Csp6I GTAC 1 cut(s) 623
CviAII CATG 5 cut(s) 178, 261, 420, 596, 751
CviJI RGCY 8 cut(s) 113, 140, 286, 359, 432, 462, 634, 740
CviKI_1 RGCY 8 cut(s) 113, 140, 286, 359, 432, 462, 634, 740
CviQI GTAC 1 cut(s) 623
DdeI CTNAG 3 cut(s) 81, 630, 657
DpnI GATC 3 cut(s) 97, 188, 760
DpnII GATC 3 cut(s) 95, 186, 758
DraIII CACNNNGTG 1 cut(s) 346
Eam1104I CTCTTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 439
Eco130I CCWWGG 2 cut(s) 177, 546
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 2 cut(s) 177, 546
ErhI CCWWGG 2 cut(s) 177, 546
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 5 cut(s) 181, 264, 423, 599, 754
FaqI GGGAC 3 cut(s) 305, 569, 679
FatI CATG 5 cut(s) 177, 260, 419, 595, 750
FblI GTMKAC 1 cut(s) 162
FokI GGATG 3 cut(s) 222, 612, 674
FspBI CTAG 4 cut(s) 5, 390, 587, 701
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 2 cut(s) 462, 740
HgaI GACGC 1 cut(s) 384
Hin1II CATG 5 cut(s) 181, 264, 423, 599, 754
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 1 cut(s) 83
HphI GGTGA 1 cut(s) 78
Hpy166II GTNNAC 2 cut(s) 163, 623
Hpy188I TCNGA 5 cut(s) 95, 268, 369, 532, 658
Hpy188III TCNNGA 8 cut(s) 21, 220, 401, 587, 596, 629, 701, 725
Hpy8I GTNNAC 2 cut(s) 163, 623
HpyAV CCTTC 2 cut(s) 295, 584
HpyCH4III ACNGT 3 cut(s) 232, 616, 670
HpyCH4V TGCA 5 cut(s) 315, 352, 580, 686, 773
HpyF3I CTNAG 3 cut(s) 81, 630, 657
Hsp92II CATG 5 cut(s) 181, 264, 423, 599, 754
Kzo9I GATC 3 cut(s) 95, 186, 758
LguI GCTCTTC 1 cut(s) 439
LweI GCATC 1 cut(s) 339
MaeI CTAG 4 cut(s) 5, 390, 587, 701
MaeIII GTNAC 5 cut(s) 48, 76, 84, 226, 607
MalI GATC 3 cut(s) 97, 188, 760
MboI GATC 3 cut(s) 95, 186, 758
MboII GAAGA 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 708
MluCI AATT 3 cut(s) 445, 478, 645
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 263
MnlI CCTC 2 cut(s) 322, 652
MseI TTAA 2 cut(s) 414, 521
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 3 cut(s) 317, 688, 817
MvaI CCWGG 1 cut(s) 59
NcoI CCATGG 1 cut(s) 177
NdeII GATC 3 cut(s) 95, 186, 758
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 181, 264, 423, 599, 754
NlaIV GGNNCC 2 cut(s) 409, 793
NmuCI GTSAC 4 cut(s) 48, 76, 84, 226
PagI TCATGA 1 cut(s) 595
PciSI GCTCTTC 1 cut(s) 439
PctI GAATGC 3 cut(s) 317, 688, 817
PflMI CCANNNNNTGG 1 cut(s) 552
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
PsiI TTATAA 2 cut(s) 131, 281
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 409, 793
PspPI GGNCC 1 cut(s) 738
PstNI CAGNNNCTG 1 cut(s) 58
RsaI GTAC 1 cut(s) 624
RsaNI GTAC 1 cut(s) 623
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 2 cut(s) 414, 521
Sau3AI GATC 3 cut(s) 95, 186, 758
Sau96I GGNCC 1 cut(s) 738
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 1 cut(s) 708
SetI ASST 9 cut(s) 115, 279, 288, 324, 519, 552, 595, 636, 771
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 1 cut(s) 798
Sse9I AATT 3 cut(s) 445, 478, 645
SspMI CTAG 4 cut(s) 5, 390, 587, 701
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 2 cut(s) 177, 546
TaaI ACNGT 3 cut(s) 232, 616, 670
TaqI TCGA 1 cut(s) 402
TasI AATT 3 cut(s) 445, 478, 645
TatI WGTACW 1 cut(s) 622
Tru1I TTAA 2 cut(s) 414, 521
Tru9I TTAA 2 cut(s) 414, 521
TscAI CASTG 4 cut(s) 85, 128, 235, 547
TseFI GTSAC 4 cut(s) 48, 76, 84, 226
Tsp45I GTSAC 4 cut(s) 48, 76, 84, 226
TspDTI ATGAA 7 cut(s) 142, 213, 484, 576, 662, 767, 824
TspRI CASTG 4 cut(s) 85, 128, 235, 547
Van91I CCANNNNNTGG 1 cut(s) 552
XapI RAATTY 1 cut(s) 445
XbaI TCTAGA 2 cut(s) 586, 700
XmiI GTMKAC 1 cut(s) 162
XspI CTAG 4 cut(s) 5, 390, 587, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.