MD06G1184200.v1.1

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004864
Physical Location & Seq
Forward (+)
32241189 .. 32243656
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1184200.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGGATTAGGATATGATAATTTTCCATGGCTGCTAAGCCTCACTGTGTGTGTGTATATATATAGAGGTTCTGCATCGTCCTTCATTCACAGCCAGTCTTACACATACATCAAGTTAACAATTTTCTTCATATTTTCTTCCTTCTATTTGCTCAAAATGGCTCTTTCACTCACTATTGAGAAAGAATTTGCTCAAAACTCAGAAAGAATTAAGTCTGTGGATCTACATCCAACTGAGCCATGGGTTCTGGTCAGTTTGTATTCAGGCGCTGTTTGTATCTGGAATTACCAATCACAGACCATGGAGAAGTCCTTCAAGGTCACTGAATCACCAGTCAGGTCAGCAAAGTTTGTAGCACGCGAACACTGGATAGTAACCGCAGCTGATGACACATTTATTCGCGTCTACAACTATGACACGACGGAAAAGATCAAAGAGTTTGAAGCACATACAGATTATATCAGGAGTGTGACTGTCCATCCTACTCTACCTTATCTCTTGTCATGCTCGGACGACAACGTTATAAAGCTTTGGGATTGGGAGAAGGATTGGTCATGCACTCAGATCTTCGAGGGACATTCTCACTATGTGATGCAAGTGGCGTTCAATCCACACGACGCCAATACTTTCTCTAGTGCGTCTCTTGATGGTACAGTCAAGGTATGGAGGATTGGGTCCGCTGCCCCGGAGTTTACATTAGAGGGCCACTTAAAAGGAGTGAATTGCGTTGATTACTTCCTTCGCGACAATAAAACATATCTATTGAGTGGCTCTGACGATTTTACTGTCAAGGTATGGGACTGTGAAACCAAAAGCTGTGTGCAAACCCTAGAAGGCCATGAGCACAATGTAACAGCTGTATGTGTTCATCCTGAGCTTCCCATAATAATCACAGCTTCTGAGGACGGGAATGTTCACATTTGGCATGCAACCACTTTTAGGCTAGAAAAGAGACTGAGCTACGATCTCGAAAGAGTTTGGGCCATCGCACACTCGGAAGGATCAGACAAGGTTGCATTTGGATTTGACAACGGAACAGTTGTAGTTAAAATGAATGGTTATGTTGATCTCCAAGCTTAG

Protein Analysis

360

Amino Acids

41.04

Weight (kDa)

5.48

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 54 - 223 6.3e-17 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 58 - 166 1.4e-11 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 61 - 227 1.6e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 62 - 178 4.6e-09 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 62 - 210 2.9e-20 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 65 - 223 7.3e-27 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 65 - 157 2.2e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 67 - 183 4e-17 Prp19 WD40 domain
WDR55 PF24796 68 - 251 2.1e-17 WDR55
WD40_CDC20-Fz PF24807 89 - 345 4.9e-27 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 110 - 183 6.6e-11 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 119 - 224 3.8e-09 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 121 - 216 3.4e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR75_1st PF23869 128 - 181 3.4e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 143 - 179 5.5e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 150 - 241 2.4e-14 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 166 - 222 1e-05 EIPR1 beta-propeller
WD40 PF00400 184 - 222 1.3e-07 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 185 - 230 3.1e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 187 - 345 2.4e-27 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 188 - 346 1.8e-20 Prp19 WD40 domain
EIF3I PF24805 190 - 278 1.5e-07 EIF3I
Beta-prop_WDR41 PF25178 205 - 310 2.6e-07 WDR41
Beta-prop_WDR5 PF25175 206 - 334 1.3e-27 WDR5 beta-propeller domain
WD40_Gbeta PF25391 206 - 308 9e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 207 - 339 7.6e-14 WDR3 second beta-propeller domain
Beta-prop_SCAP PF24017 212 - 311 4.9e-08 SCAP Beta-propeller
Beta-prop_EML PF23409 216 - 347 6.9e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 229 - 267 1.7e-08 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 232 - 320 1.4e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 239 - 308 3.6e-06 WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st PF24817 247 - 340 3.8e-14 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 250 - 319 1.1e-07 WD repeat-containing protein 75 first beta-propeller
WDR55 PF24796 250 - 348 1.2e-09 WDR55
Beta-prop_THOC3 PF25174 250 - 347 9.4e-15 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 251 - 313 2.5e-07 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 257 - 313 2.2e-08 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 272 - 308 3e-09 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 285 - 349 8e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AccI GTMKAC 1 cut(s) 405
AccII CGCG 3 cut(s) 360, 402, 744
AciI CCGC 2 cut(s) 378, 678
AclI AACGTT 1 cut(s) 519
AclWI GGATC 2 cut(s) 228, 1009
AcsI RAATTY 1 cut(s) 185
AcyI GRCGYC 1 cut(s) 618
AdeI CACNNNGTG 2 cut(s) 48, 589
AfaI GTAC 1 cut(s) 652
AfiI CCNNNNNNNGG 1 cut(s) 939
AgsI TTSAA 3 cut(s) 316, 443, 607
AluBI AGCT 8 cut(s) 383, 529, 816, 857, 877, 896, 960, 1076
AluI AGCT 8 cut(s) 383, 529, 816, 857, 877, 896, 960, 1076
Alw21I GWGCWC 1 cut(s) 846
Alw26I GTCTC 2 cut(s) 645, 946
AlwI GGATC 2 cut(s) 228, 1009
AlwNI CAGNNNCTG 2 cut(s) 269, 899
AoxI GGCC 3 cut(s) 703, 835, 981
ApeKI GCWGC 3 cut(s) 31, 380, 680
ApoI RAATTY 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 3 cut(s) 675, 703, 981
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 1 cut(s) 321
AvaII GGWCC 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 846
BbvI GCAGC 3 cut(s) 18, 392, 667
BccI CCATC 3 cut(s) 486, 641, 992
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 2 cut(s) 645, 946
BfaI CTAG 3 cut(s) 633, 830, 944
BfoI RGCGCY 1 cut(s) 270
BglII AGATCT 1 cut(s) 564
BisI GCNGC 3 cut(s) 32, 381, 681
BlpI GCTNAGC 1 cut(s) 35
BlsI GCNGC 3 cut(s) 33, 382, 682
Bme1390I CCNGG 1 cut(s) 686
Bme18I GGWCC 1 cut(s) 675
BmgT120I GGNCC 3 cut(s) 675, 703, 981
BmiI GGNNCC 1 cut(s) 676
BmrFI CCNGG 1 cut(s) 686
BmsI GCATC 2 cut(s) 83, 582
Bpu10I CCTNAGC 1 cut(s) 873
Bpu1102I GCTNAGC 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 686
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 4 cut(s) 26, 239, 300, 684
Bsc4I CCNNNNNNNGG 1 cut(s) 939
Bse1I ACTGG 3 cut(s) 94, 332, 371
Bse8I GATNNNNATC 1 cut(s) 225
BseDI CCNNGG 4 cut(s) 26, 239, 300, 684
BseGI GGATG 3 cut(s) 226, 478, 868
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 939
BseMII CTCAG 6 cut(s) 213, 225, 575, 864, 891, 947
BseNI ACTGG 3 cut(s) 94, 332, 371
BseXI GCAGC 3 cut(s) 18, 392, 667
Bsh1236I CGCG 3 cut(s) 360, 402, 744
BshFI GGCC 3 cut(s) 705, 837, 983
BsiHKAI GWGCWC 1 cut(s) 846
BsiSI CCGG 1 cut(s) 686
BslFI GGGAC 2 cut(s) 588, 812
BslI CCNNNNNNNGG 1 cut(s) 939
BsmAI GTCTC 2 cut(s) 645, 946
BsmBI CGTCTC 1 cut(s) 645
BsmFI GGGAC 2 cut(s) 588, 812
BsnI GGCC 3 cut(s) 705, 837, 983
Bsp1286I GDGCHC 1 cut(s) 846
Bsp143I GATC 6 cut(s) 220, 429, 564, 964, 1001, 1066
Bsp1720I GCTNAGC 1 cut(s) 35
Bsp19I CCATGG 3 cut(s) 26, 239, 300
Bsp68I TCGCGA 1 cut(s) 744
BspACI CCGC 2 cut(s) 378, 678
BspANI GGCC 3 cut(s) 705, 837, 983
BspCNI CTCAG 6 cut(s) 212, 226, 574, 865, 892, 948
BspFNI CGCG 3 cut(s) 360, 402, 744
BspLI GGNNCC 1 cut(s) 676
BspPI GGATC 2 cut(s) 228, 1009
BsrI ACTGG 3 cut(s) 94, 332, 371
BssECI CCNNGG 4 cut(s) 26, 239, 300, 684
BssMI GATC 6 cut(s) 220, 429, 564, 964, 1001, 1066
BssNI GRCGYC 1 cut(s) 618
BssT1I CCWWGG 3 cut(s) 26, 239, 300
Bst4CI ACNGT 6 cut(s) 46, 475, 655, 787, 803, 1039
BstACI GRCGYC 1 cut(s) 618
BstC8I GCNNGC 2 cut(s) 358, 927
BstDEI CTNAG 8 cut(s) 35, 199, 234, 561, 873, 900, 956, 1077
BstDSI CCRYGG 3 cut(s) 26, 239, 300
BstF5I GGATG 3 cut(s) 226, 478, 868
BstFNI CGCG 3 cut(s) 360, 402, 744
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 6 cut(s) 223, 432, 567, 967, 1004, 1069
BstMAI GTCTC 2 cut(s) 645, 946
BstMBI GATC 6 cut(s) 220, 429, 564, 964, 1001, 1066
BstNSI RCATGY 1 cut(s) 929
BstSCI CCNGG 1 cut(s) 684
BstUI CGCG 3 cut(s) 360, 402, 744
BstV1I GCAGC 3 cut(s) 18, 392, 667
BstX2I RGATCY 2 cut(s) 220, 564
BstYI RGATCY 2 cut(s) 220, 564
BsuRI GGCC 3 cut(s) 705, 837, 983
BtgI CCRYGG 3 cut(s) 26, 239, 300
BtgZI GCGATG 1 cut(s) 970
BtsCI GGATG 3 cut(s) 226, 478, 868
BtsIMutI CAGTG 3 cut(s) 42, 321, 364
BtuMI TCGCGA 1 cut(s) 744
Cac8I GCNNGC 2 cut(s) 358, 927
CaiI CAGNNNCTG 2 cut(s) 269, 899
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 675, 703, 981
CseI GACGC 3 cut(s) 391, 626, 627
Csp6I GTAC 1 cut(s) 651
CviAII CATG 7 cut(s) 27, 240, 301, 504, 555, 839, 926
CviQI GTAC 1 cut(s) 651
DdeI CTNAG 8 cut(s) 35, 199, 234, 561, 873, 900, 956, 1077
DpnI GATC 6 cut(s) 222, 431, 566, 966, 1003, 1068
DpnII GATC 6 cut(s) 220, 429, 564, 964, 1001, 1066
DraIII CACNNNGTG 2 cut(s) 48, 589
Eco130I CCWWGG 3 cut(s) 26, 239, 300
Eco47I GGWCC 1 cut(s) 675
EcoT14I CCWWGG 3 cut(s) 26, 239, 300
ErhI CCWWGG 3 cut(s) 26, 239, 300
Esp3I CGTCTC 1 cut(s) 645
FaeI CATG 7 cut(s) 30, 243, 304, 507, 558, 842, 929
FaqI GGGAC 2 cut(s) 588, 812
FatI CATG 7 cut(s) 26, 239, 300, 503, 554, 838, 925
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 3 cut(s) 32, 381, 681
FokI GGATG 3 cut(s) 213, 465, 855
Fsp4HI GCNGC 3 cut(s) 32, 381, 681
FspBI CTAG 3 cut(s) 633, 830, 944
GlaI GCGC 1 cut(s) 268
GluI GCNGC 3 cut(s) 32, 381, 681
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 705, 837, 983
HapII CCGG 1 cut(s) 686
HgaI GACGC 3 cut(s) 391, 626, 627
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 7 cut(s) 30, 243, 304, 507, 558, 842, 929
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HindIII AAGCTT 2 cut(s) 527, 1074
HinfI GANTC 1 cut(s) 326
HpaI GTTAAC 1 cut(s) 117
HpaII CCGG 1 cut(s) 686
HphI GGTGA 1 cut(s) 321
Hpy166II GTNNAC 4 cut(s) 117, 406, 693, 916
Hpy188I TCNGA 7 cut(s) 202, 511, 564, 775, 901, 997, 1006
Hpy188III TCNNGA 6 cut(s) 280, 463, 644, 743, 872, 968
Hpy8I GTNNAC 4 cut(s) 117, 406, 693, 916
Hpy99I CGWCG 2 cut(s) 424, 620
HpyAV CCTTC 7 cut(s) 91, 151, 322, 538, 749, 827, 992
HpyCH4III ACNGT 6 cut(s) 46, 475, 655, 787, 803, 1039
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 6 cut(s) 74, 558, 595, 823, 929, 1016
HpyF3I CTNAG 8 cut(s) 35, 199, 234, 561, 873, 900, 956, 1077
HpySE526I ACGT 1 cut(s) 519
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 7 cut(s) 30, 243, 304, 507, 558, 842, 929
HspAI GCGC 1 cut(s) 267
KspAI GTTAAC 1 cut(s) 117
Kzo9I GATC 6 cut(s) 220, 429, 564, 964, 1001, 1066
Lsp1109I GCAGC 3 cut(s) 18, 392, 667
LweI GCATC 2 cut(s) 83, 582
MaeI CTAG 3 cut(s) 633, 830, 944
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 4 cut(s) 319, 373, 469, 850
MalI GATC 6 cut(s) 222, 431, 566, 966, 1003, 1068
MboI GATC 6 cut(s) 220, 429, 564, 964, 1001, 1066
MboII GAAGA 3 cut(s) 118, 129, 559
MflI RGATCY 2 cut(s) 220, 564
MhlI GDGCHC 1 cut(s) 846
MluCI AATT 6 cut(s) 19, 120, 185, 207, 283, 721
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 6 cut(s) 50, 59, 565, 660, 694, 895
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 4 cut(s) 116, 210, 710, 1047
MspA1I CMGCKG 3 cut(s) 383, 680, 857
MspI CCGG 1 cut(s) 686
MspR9I CCNGG 1 cut(s) 686
MvnI CGCG 3 cut(s) 360, 402, 744
NciI CCSGG 1 cut(s) 686
NcoI CCATGG 3 cut(s) 26, 239, 300
NdeII GATC 6 cut(s) 220, 429, 564, 964, 1001, 1066
NlaIII CATG 7 cut(s) 30, 243, 304, 507, 558, 842, 929
NlaIV GGNNCC 1 cut(s) 676
NmuCI GTSAC 2 cut(s) 319, 469
NruI TCGCGA 1 cut(s) 744
NspI RCATGY 1 cut(s) 929
PaeI GCATGC 1 cut(s) 929
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 3 cut(s) 33, 382, 682
PsiI TTATAA 1 cut(s) 524
Psp1406I AACGTT 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 676
PspPI GGNCC 3 cut(s) 675, 703, 981
PstNI CAGNNNCTG 2 cut(s) 269, 899
PsuI RGATCY 2 cut(s) 220, 564
PvuII CAGCTG 2 cut(s) 383, 857
RruI TCGCGA 1 cut(s) 744
RsaI GTAC 1 cut(s) 652
RsaNI GTAC 1 cut(s) 651
SaqAI TTAA 4 cut(s) 116, 210, 710, 1047
SatI GCNGC 3 cut(s) 32, 381, 681
Sau3AI GATC 6 cut(s) 220, 429, 564, 964, 1001, 1066
Sau96I GGNCC 3 cut(s) 675, 703, 981
ScrFI CCNGG 1 cut(s) 686
SduI GDGCHC 1 cut(s) 846
SfaNI GCATC 2 cut(s) 83, 582
SinI GGWCC 1 cut(s) 675
SphI GCATGC 1 cut(s) 929
Sse9I AATT 6 cut(s) 19, 120, 185, 207, 283, 721
SsiI CCGC 2 cut(s) 378, 678
SspMI CTAG 3 cut(s) 633, 830, 944
StyD4I CCNGG 1 cut(s) 684
StyI CCWWGG 3 cut(s) 26, 239, 300
TaaI ACNGT 6 cut(s) 46, 475, 655, 787, 803, 1039
TaiI ACGT 1 cut(s) 522
TaqI TCGA 2 cut(s) 570, 969
TasI AATT 6 cut(s) 19, 120, 185, 207, 283, 721
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 4 cut(s) 116, 210, 710, 1047
Tru9I TTAA 4 cut(s) 116, 210, 710, 1047
TscAI CASTG 3 cut(s) 49, 328, 371
TseFI GTSAC 2 cut(s) 319, 469
TseI GCWGC 3 cut(s) 31, 380, 680
Tsp45I GTSAC 2 cut(s) 319, 469
TspDTI ATGAA 4 cut(s) 73, 118, 857, 1067
TspGWI ACGGA 2 cut(s) 437, 1047
TspRI CASTG 3 cut(s) 49, 328, 371
VpaK11BI GGWCC 1 cut(s) 675
XapI RAATTY 1 cut(s) 185
XceI RCATGY 1 cut(s) 929
XcmI CCANNNNNNNNNTGG 1 cut(s) 237
XmiI GTMKAC 1 cut(s) 405
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 3 cut(s) 633, 830, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.