FvH4_6g33500

mediator of RNA polymerase II transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013345
Physical Location & Seq
Reverse (-)
26522589 .. 26524463
1875 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g33500.t1

Sequence Viewer

Length: 522 bp
ATGGAGCACCAGTACTCGGGCGGAGGAGGAGGAGGAGGGAGCGGGAGCTGGAATATGATCCCCAGCGTGCCGACCCACAGTAACCCATCTACGCCATCCAGTCAGGACCATCTTTACCTCCAACAACACCAACACCAATTTCAGCAGCAGCAACAACAACAGCGTCTCCTTCTCCAACAACAACAGCAACAGCAAAATCAACAGCAGCAGCAGCAACAACAACAGCATCAGTCTCTCGCCTCCCACTTCCATCTCTTCCACTTGGTGGAGAAGTTATCAGATTCGGTTGAGACCGGGACTAGGGACCCGCAGTCTGATGCATTGATTAGTGATTTGAACAACCATTTCGAGAAGTGCCAGCAGCTGTTGAACTCCATATCGGGATCCATTGCCACCAAGGCTATGACGGTGGAGGGACAGAAGCGGAAGCTCGAAGAGAGCGAGCAGTTGTTAAATCAGCGGAGGGACCTGAATACTAAGTACAGAAATTCTGTTGTTGACCTCCTCAAGTCTGAGCCTTAG

Protein Analysis

174

Amino Acids

19.73

Weight (kDa)

6.34

Isoelectric Point (pI)

62.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29580 AT1G55080 AT1G55080
fragaria_vesca FvH4_6g33500
malus_domestica MD09G1192700.v1.1
prunus_persica Prupe.3G049000_v2.0.a1
pyrus_communis pycom09g10920
rosa_chinensis RchiOBHm_Chr2g0143361
rosa_laevigata RLG00000020032
rosa_multiflora Rmu_sc0003507.1_g000018 Rmu_sc0024801.1_g000003
rosa_roxburghii Rroxscaffold_2G00101930
rosa_rugosa Rorug02G0380900
rosa_samantha Rh2AG433800 Rh2BG441800 Rh2CG419500 Rh2DG451900
rosa_wichuraiana Rw2G035370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 42
AciI CCGC 5 cut(s) 21, 42, 308, 424, 460
AclWI GGATC 3 cut(s) 52, 378, 391
AcsI RAATTY 1 cut(s) 487
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 2 cut(s) 14, 482
AfiI CCNNNNNNNGG 3 cut(s) 16, 265, 300
AgsI TTSAA 2 cut(s) 337, 370
AhdI GACNNNNNGTC 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
AluBI AGCT 3 cut(s) 48, 364, 430
AluI AGCT 3 cut(s) 48, 364, 430
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 3 cut(s) 170, 237, 284
AlwI GGATC 3 cut(s) 52, 378, 391
AlwNI CAGNNNCTG 1 cut(s) 364
Ama87I CYCGRG 1 cut(s) 16
ApeKI GCWGC 6 cut(s) 145, 148, 205, 208, 211, 361
ApoI RAATTY 1 cut(s) 487
AspS9I GGNCC 3 cut(s) 106, 304, 466
AsuC2I CCSGG 1 cut(s) 295
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 3 cut(s) 106, 304, 466
BamHI GGATCC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 6 cut(s) 157, 160, 217, 220, 223, 373
BccI CCATC 4 cut(s) 94, 103, 117, 258
BcnI CCSGG 1 cut(s) 295
BcoDI GTCTC 3 cut(s) 170, 237, 284
BfaI CTAG 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 6 cut(s) 146, 149, 206, 209, 212, 362
BlsI GCNGC 6 cut(s) 147, 150, 207, 210, 213, 363
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 295
Bme18I GGWCC 3 cut(s) 106, 304, 466
BmeRI GACNNNNNGTC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 16
BmgT120I GGNCC 3 cut(s) 106, 304, 466
BmiI GGNNCC 4 cut(s) 305, 306, 385, 467
BmrFI CCNGG 1 cut(s) 295
BmsI GCATC 2 cut(s) 235, 307
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 295
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 1 cut(s) 396
BsaXI ACNNNNNCTCC 3 cut(s) 26, 150, 180
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 265, 300
Bse1I ACTGG 2 cut(s) 10, 99
Bse3DI GCAATG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 396
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 3 cut(s) 16, 265, 300
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 2 cut(s) 10, 99
BseRI GAGGAG 5 cut(s) 39, 42, 45, 48, 494
BseXI GCAGC 6 cut(s) 157, 160, 217, 220, 223, 373
BseYI CCCAGC 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 4 cut(s) 310, 317, 429, 479
BslI CCNNNNNNNGG 3 cut(s) 16, 265, 300
BsmAI GTCTC 3 cut(s) 170, 237, 284
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 4 cut(s) 310, 317, 429, 479
Bso31I GGTCTC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 2 cut(s) 57, 383
BspACI CCGC 5 cut(s) 21, 42, 308, 424, 460
BspCNI CTCAG 1 cut(s) 505
BspLI GGNNCC 4 cut(s) 305, 306, 385, 467
BspPI GGATC 3 cut(s) 52, 378, 391
BspTNI GGTCTC 1 cut(s) 284
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 2 cut(s) 10, 99
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 2 cut(s) 57, 383
BssT1I CCWWGG 1 cut(s) 396
Bst4CI ACNGT 2 cut(s) 80, 409
Bst6I CTCTTC 2 cut(s) 260, 429
BstC8I GCNNGC 3 cut(s) 68, 359, 443
BstDEI CTNAG 3 cut(s) 477, 513, 519
BstF5I GGATG 1 cut(s) 95
BstKTI GATC 2 cut(s) 60, 386
BstMAI GTCTC 3 cut(s) 170, 237, 284
BstMBI GATC 2 cut(s) 57, 383
BstMWI GCNNNNNNNGC 2 cut(s) 211, 398
BstSCI CCNGG 1 cut(s) 293
BstV1I GCAGC 6 cut(s) 157, 160, 217, 220, 223, 373
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BtsCI GGATG 1 cut(s) 95
Cac8I GCNNGC 3 cut(s) 68, 359, 443
CaiI CAGNNNCTG 1 cut(s) 364
Cfr13I GGNCC 3 cut(s) 106, 304, 466
CseI GACGC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 13, 481
CviJI RGCY 5 cut(s) 48, 364, 401, 430, 517
CviKI_1 RGCY 5 cut(s) 48, 364, 401, 430, 517
CviQI GTAC 2 cut(s) 13, 481
DdeI CTNAG 3 cut(s) 477, 513, 519
DpnI GATC 2 cut(s) 59, 385
DpnII GATC 2 cut(s) 57, 383
DraIII CACNNNGTG 1 cut(s) 265
DriI GACNNNNNGTC 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 260, 429
Eam1105I GACNNNNNGTC 1 cut(s) 310
EarI CTCTTC 2 cut(s) 260, 429
EciI GGCGGA 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 396
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 3 cut(s) 106, 304, 466
Eco88I CYCGRG 1 cut(s) 16
EcoO109I RGGNCCY 2 cut(s) 304, 466
EcoT14I CCWWGG 1 cut(s) 396
EcoT22I ATGCAT 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 170
FaiI YATR 3 cut(s) 56, 377, 404
FaqI GGGAC 4 cut(s) 310, 317, 429, 479
FauI CCCGC 2 cut(s) 35, 315
Fnu4HI GCNGC 6 cut(s) 146, 149, 206, 209, 212, 362
FokI GGATG 1 cut(s) 82
Fsp4HI GCNGC 6 cut(s) 146, 149, 206, 209, 212, 362
FspBI CTAG 1 cut(s) 300
GluI GCNGC 6 cut(s) 146, 149, 206, 209, 212, 362
GsaI CCCAGC 1 cut(s) 66
HapII CCGG 1 cut(s) 294
HgaI GACGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 1 cut(s) 281
HpaII CCGG 1 cut(s) 294
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 3 cut(s) 280, 316, 514
Hpy188III TCNNGA 3 cut(s) 104, 349, 381
Hpy8I GTNNAC 1 cut(s) 499
HpyAV CCTTC 1 cut(s) 179
HpyCH4III ACNGT 2 cut(s) 80, 409
HpyCH4V TGCA 1 cut(s) 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 211, 398
HpyF3I CTNAG 3 cut(s) 477, 513, 519
KflI GGGWCCC 1 cut(s) 304
Kzo9I GATC 2 cut(s) 57, 383
LmnI GCTCC 3 cut(s) 4, 39, 45
LpnPI CCDG 8 cut(s) 23, 34, 76, 89, 112, 307, 371, 482
Lsp1109I GCAGC 6 cut(s) 157, 160, 217, 220, 223, 373
LweI GCATC 2 cut(s) 235, 307
MaeI CTAG 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 80
MalI GATC 2 cut(s) 59, 385
MbiI CCGCTC 1 cut(s) 42
MboI GATC 2 cut(s) 57, 383
MboII GAAGA 2 cut(s) 247, 446
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 2 cut(s) 137, 487
MmeI TCCRAC 2 cut(s) 145, 199
Mph1103I ATGCAT 1 cut(s) 322
MseI TTAA 1 cut(s) 452
MspA1I CMGCKG 2 cut(s) 364, 460
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 295
MwoI GCNNNNNNNGC 2 cut(s) 211, 398
NciI CCSGG 1 cut(s) 295
NdeII GATC 2 cut(s) 57, 383
NlaIV GGNNCC 4 cut(s) 305, 306, 385, 467
NsiI ATGCAT 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PfeI GAWTC 1 cut(s) 281
PflMI CCANNNNNTGG 1 cut(s) 265
PkrI GCNGC 6 cut(s) 147, 150, 207, 210, 213, 363
PpuMI RGGWCCY 2 cut(s) 304, 466
Psp5II RGGWCCY 2 cut(s) 304, 466
PspFI CCCAGC 1 cut(s) 62
PspN4I GGNNCC 4 cut(s) 305, 306, 385, 467
PspPI GGNCC 3 cut(s) 106, 304, 466
PspPPI RGGWCCY 2 cut(s) 304, 466
PstNI CAGNNNCTG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 364
RsaI GTAC 2 cut(s) 14, 482
RsaNI GTAC 2 cut(s) 13, 481
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 6 cut(s) 146, 149, 206, 209, 212, 362
Sau3AI GATC 2 cut(s) 57, 383
Sau96I GGNCC 3 cut(s) 106, 304, 466
ScaI AGTACT 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 1 cut(s) 9
SetI ASST 6 cut(s) 50, 120, 366, 432, 471, 504
SfaNI GCATC 2 cut(s) 235, 307
SinI GGWCC 3 cut(s) 106, 304, 466
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 2 cut(s) 137, 487
SsiI CCGC 5 cut(s) 21, 42, 308, 424, 460
SspMI CTAG 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 293
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 2 cut(s) 80, 409
TaqI TCGA 2 cut(s) 348, 432
TasI AATT 2 cut(s) 137, 487
TatI WGTACW 2 cut(s) 12, 480
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TseI GCWGC 6 cut(s) 145, 148, 205, 208, 211, 361
Van91I CCANNNNNTGG 1 cut(s) 265
VpaK11BI GGWCC 3 cut(s) 106, 304, 466
XapI RAATTY 1 cut(s) 487
XspI CTAG 1 cut(s) 300
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.