Rw2G035370

mediator of RNA polymerase II transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013345
Physical Location & Seq
Reverse (-)
58313280 .. 58315198
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G035370.1

Sequence Viewer

Length: 525 bp
ATGGAGCATCAGTACTCGGGCGGAGGAGGAGGAGGAAGTGGGAGCTGGAATATGATCCCCAGCGTGCCGACCCACAGTAACCCTTCTACGCCGTCCAGTCAGGACCATCTCTACCTCCAACAACACCAACAGCAATTCCAGCAACAACAACAACAACAGCGTCTTCTTCTCCAACAACAACAACAACAACAGCAGCAGCAACAACAGCAACAGCAACAACAACAACAGCATCAGTCTCTCGCCTCCCACTTCCATCTCTTCCACTTGGTGGAGAAGTTATCGGATTCGATTGAGAACGGGACGAGGGATCAGCAATCGGATGCATTGGTCGCTGATTTGAACAACCATTTCGACAAGTGCCAGCAGCTGTTGAACTCCATATCCGGATCCATCACCACCAAGGCTATGACGGTTGAGGGACAGAAGAAGAAGCTTGAAGAGAGTGAGCAATTGTTAAATCAGCGGAGGGACCTGAATAACAAGTACAAAAACTCTGTTGTTGACCTCCTCAAGTCTGAGCCGTAG

Protein Analysis

174

Amino Acids

19.94

Weight (kDa)

6.28

Isoelectric Point (pI)

70.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29580 AT1G55080 AT1G55080
fragaria_vesca FvH4_6g33500
malus_domestica MD09G1192700.v1.1
prunus_persica Prupe.3G049000_v2.0.a1
pyrus_communis pycom09g10920
rosa_chinensis RchiOBHm_Chr2g0143361
rosa_laevigata RLG00000020032
rosa_multiflora Rmu_sc0003507.1_g000018 Rmu_sc0024801.1_g000003
rosa_roxburghii Rroxscaffold_2G00101930
rosa_rugosa Rorug02G0380900
rosa_samantha Rh2AG433800 Rh2BG441800 Rh2CG419500 Rh2DG451900
rosa_wichuraiana Rw2G035370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 268
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 2 cut(s) 21, 463
AclWI GGATC 4 cut(s) 49, 315, 381, 394
AdeI CACNNNGTG 1 cut(s) 268
AfaI GTAC 2 cut(s) 14, 485
AfiI CCNNNNNNNGG 1 cut(s) 268
AgsI TTSAA 3 cut(s) 340, 373, 437
AloI GAACNNNNNNTCC 2 cut(s) 365, 397
AluBI AGCT 3 cut(s) 45, 367, 433
AluI AGCT 3 cut(s) 45, 367, 433
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 4 cut(s) 49, 315, 381, 394
AlwNI CAGNNNCTG 1 cut(s) 367
Ama87I CYCGRG 1 cut(s) 16
Aor13HI TCCGGA 1 cut(s) 383
ApeKI GCWGC 3 cut(s) 193, 196, 364
AspS9I GGNCC 2 cut(s) 103, 469
AsuHPI GGTGA 1 cut(s) 385
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 2 cut(s) 103, 469
BamHI GGATCC 1 cut(s) 386
BbsI GAAGAC 1 cut(s) 155
BbvI GCAGC 3 cut(s) 205, 208, 376
BccI CCATC 3 cut(s) 114, 261, 398
BceAI ACGGC 2 cut(s) 76, 505
BcoDI GTCTC 1 cut(s) 240
BisI GCNGC 3 cut(s) 194, 197, 365
BlsI GCNGC 3 cut(s) 195, 198, 366
BmcAI AGTACT 1 cut(s) 14
Bme18I GGWCC 2 cut(s) 103, 469
BmeT110I CYCGRG 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 103, 469
BmiI GGNNCC 2 cut(s) 388, 470
BmsI GCATC 3 cut(s) 16, 238, 310
BpiI GAAGAC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 494
BsaJI CCNNGG 1 cut(s) 399
BsaWI WCCGGW 1 cut(s) 383
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 96
BseAI TCCGGA 1 cut(s) 383
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 96
BseRI GAGGAG 4 cut(s) 39, 42, 45, 497
BseXI GCAGC 3 cut(s) 205, 208, 376
BseYI CCCAGC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 1 cut(s) 384
BslFI GGGAC 3 cut(s) 313, 432, 482
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 1 cut(s) 240
BsmFI GGGAC 3 cut(s) 313, 432, 482
BsoBI CYCGRG 1 cut(s) 16
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 3 cut(s) 54, 307, 386
BspACI CCGC 2 cut(s) 21, 463
BspCNI CTCAG 1 cut(s) 508
BspEI TCCGGA 1 cut(s) 383
BspLI GGNNCC 2 cut(s) 388, 470
BspPI GGATC 4 cut(s) 49, 315, 381, 394
BsrI ACTGG 1 cut(s) 96
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 3 cut(s) 54, 307, 386
BssT1I CCWWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 77, 412
Bst6I CTCTTC 2 cut(s) 263, 432
BstC8I GCNNGC 2 cut(s) 65, 362
BstDEI CTNAG 1 cut(s) 516
BstF5I GGATG 1 cut(s) 325
BstKTI GATC 3 cut(s) 57, 310, 389
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 3 cut(s) 54, 307, 386
BstMWI GCNNNNNNNGC 3 cut(s) 139, 205, 329
BstV1I GCAGC 3 cut(s) 205, 208, 376
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
BtsCI GGATG 1 cut(s) 325
Cac8I GCNNGC 2 cut(s) 65, 362
CaiI CAGNNNCTG 1 cut(s) 367
Cfr13I GGNCC 2 cut(s) 103, 469
CseI GACGC 1 cut(s) 149
Csp6I GTAC 2 cut(s) 13, 484
CviJI RGCY 5 cut(s) 45, 367, 404, 433, 520
CviKI_1 RGCY 5 cut(s) 45, 367, 404, 433, 520
CviQI GTAC 2 cut(s) 13, 484
DdeI CTNAG 1 cut(s) 516
DpnI GATC 3 cut(s) 56, 309, 388
DpnII GATC 3 cut(s) 54, 307, 386
DraIII CACNNNGTG 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 263, 432
EarI CTCTTC 2 cut(s) 263, 432
EciI GGCGGA 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 399
Eco47I GGWCC 2 cut(s) 103, 469
Eco88I CYCGRG 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 399
EcoT22I ATGCAT 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 399
FaiI YATR 3 cut(s) 53, 380, 407
FaqI GGGAC 3 cut(s) 313, 432, 482
Fnu4HI GCNGC 3 cut(s) 194, 197, 365
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 3 cut(s) 194, 197, 365
GluI GCNGC 3 cut(s) 194, 197, 365
GsaI CCCAGC 1 cut(s) 63
HapII CCGG 1 cut(s) 384
HgaI GACGC 1 cut(s) 149
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 1 cut(s) 284
HpaII CCGG 1 cut(s) 384
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 1 cut(s) 502
Hpy188I TCNGA 3 cut(s) 283, 319, 517
Hpy188III TCNNGA 2 cut(s) 101, 384
Hpy8I GTNNAC 1 cut(s) 502
HpyAV CCTTC 1 cut(s) 93
HpyCH4III ACNGT 2 cut(s) 77, 412
HpyCH4V TGCA 1 cut(s) 323
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 205, 329
HpyF3I CTNAG 1 cut(s) 516
Kpn2I TCCGGA 1 cut(s) 383
Kzo9I GATC 3 cut(s) 54, 307, 386
LmnI GCTCC 2 cut(s) 4, 42
LpnPI CCDG 8 cut(s) 31, 73, 86, 109, 152, 374, 397, 485
Lsp1109I GCAGC 3 cut(s) 205, 208, 376
LweI GCATC 3 cut(s) 16, 238, 310
MaeIII GTNAC 1 cut(s) 77
MalI GATC 3 cut(s) 56, 309, 388
MboI GATC 3 cut(s) 54, 307, 386
MboII GAAGA 6 cut(s) 155, 158, 250, 436, 439, 449
MfeI CAATTG 1 cut(s) 449
MflI RGATCY 1 cut(s) 386
MluCI AATT 2 cut(s) 134, 449
MmeI TCCRAC 2 cut(s) 142, 196
Mph1103I ATGCAT 1 cut(s) 325
MroI TCCGGA 1 cut(s) 383
MseI TTAA 1 cut(s) 455
MspA1I CMGCKG 2 cut(s) 367, 463
MspI CCGG 1 cut(s) 384
MunI CAATTG 1 cut(s) 449
MwoI GCNNNNNNNGC 3 cut(s) 139, 205, 329
NdeII GATC 3 cut(s) 54, 307, 386
NlaIV GGNNCC 2 cut(s) 388, 470
NsiI ATGCAT 1 cut(s) 325
PfeI GAWTC 1 cut(s) 284
PflMI CCANNNNNTGG 1 cut(s) 268
PkrI GCNGC 3 cut(s) 195, 198, 366
PpuMI RGGWCCY 1 cut(s) 469
Psp5II RGGWCCY 1 cut(s) 469
PspFI CCCAGC 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 388, 470
PspPI GGNCC 2 cut(s) 103, 469
PspPPI RGGWCCY 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 386
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 2 cut(s) 14, 485
RsaNI GTAC 2 cut(s) 13, 484
SaqAI TTAA 1 cut(s) 455
SatI GCNGC 3 cut(s) 194, 197, 365
Sau3AI GATC 3 cut(s) 54, 307, 386
Sau96I GGNCC 2 cut(s) 103, 469
ScaI AGTACT 1 cut(s) 14
SetI ASST 6 cut(s) 47, 117, 369, 435, 474, 507
SfaNI GCATC 3 cut(s) 16, 238, 310
SinI GGWCC 2 cut(s) 103, 469
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 2 cut(s) 134, 449
SsiI CCGC 2 cut(s) 21, 463
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 77, 412
TaqI TCGA 2 cut(s) 287, 351
TasI AATT 2 cut(s) 134, 449
TatI WGTACW 2 cut(s) 12, 483
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 1 cut(s) 455
Tru9I TTAA 1 cut(s) 455
TseI GCWGC 3 cut(s) 193, 196, 364
Van91I CCANNNNNTGG 1 cut(s) 268
VpaK11BI GGWCC 2 cut(s) 103, 469
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.