Rorug02G0380900

mediator of RNA polymerase II transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013345
Physical Location & Seq
Reverse (-)
48626838 .. 48629867
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0380900.1

Sequence Viewer

Length: 1323 bp
ATGGCGGTGATTTCAGTGAAGCCCGTGTTCACCGTAGTGGTGGTCACTCTCTCTCTCATCCTCGCCGTCCTTGTCTTCTCCCCTACTTCGCCTTTCTACCAATCCCCTCTCTCTCTTGCTTCACCCCAAAGAAAATTGGATATATGGAGTGTTGGGAGGATCGTAGAGTGGAGACCTTGCAAGTGGTGGCTACAAGGACATCTCACCGCTCTGCCATCAAAGAGTAATGGATTCATTCGAGTGGATTGCTATGGTGGCCTTAATCAGATGAGAAGAGATTTGTGTGATGGGGTCGGTATTGCCCGTCTTTTAAATGCAACCCTTGTTCTGCCAAAGTTTGAGGTGGCTGCTTATTGGAATGAATCAAGTGGTTTTGCAGACGTCTTTGATGTTGACTACTTCATTCAACAGTTGAACGGCTTTATCAGAGTTGTCAAAGAGTTACCACATGAGTTTGTATCAAAAGAACCCTTCCGTGTAGATTGCAGCAAACGTAAAGGCCAATTTGATTATATTGAAAATGTTCTTCCGTCTTTATTAGAACATCATTATATATCCATCACACCAGCAATGAGCCAAAGAAGGGATAGGTATCCTCAGTTTGCAAAAGCTGCCCTTTGTCAAGCATGTTACAGTGCATTACGCCTCACGAAAGCCCTGGAGAAGAAGGCTTCTGAGCTTCTTGAAGCCATACCTAAACCTTTTCTGTCACTTCACCTTCGTTTTGAGCCTGATATGGTAGCCTACAGTCAATGTGAATACCCATACCTTTCTCCTGCCTCCATTGAAGCTGTTAAGGCAGCACAAGGTGATAGAAAACCTTGGACTGGGGAGCTTGCCCAAATTTGGAGGAAACGAGGGAAATGTCCTCTTACACCTAATGAGACAGCATTCATACTCCAAGTGCTTTCCATCCCAACCAACACAAACATTTATCTTGCAGCTGGGGATGGCCTGATGGAAATTGAAGGATTAACATCTGTTTACACAAATGTAGTTACCAAGTCTAGCCTACTTAGTGGGGAGGATTTCAAAAGCATGCATGGGAACACCAAAGCTGCTTTGGATTATTATGTGTCTATTAACAGTGATTCATATATAGCAACATATTTTGGAAATATGGATAAGATGGTTGCAGCAATGCGAGCTTATAATGGCTTGTACAAGACTCTTTTCTTAAGCAGAAGAGCTTTTGCAGAGTACACGTCTCAAGGTTTGATAGGGAAGGAATTGATGCAAGCCCTATGGAAGACTCATAGAGATGATTTTGTCATGGGAAGAGGATCTGCTTTGCCTGATTGCTTCTGTGAGTTCAAATTGTGA

Protein Analysis

440

Amino Acids

49.6

Weight (kDa)

8.75

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 78 - 385 8.5e-63 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29580 AT1G55080 AT1G55080
fragaria_vesca FvH4_6g33500
malus_domestica MD09G1192700.v1.1
prunus_persica Prupe.3G049000_v2.0.a1
pyrus_communis pycom09g10920
rosa_chinensis RchiOBHm_Chr2g0143361
rosa_laevigata RLG00000020032
rosa_multiflora Rmu_sc0003507.1_g000018 Rmu_sc0024801.1_g000003
rosa_roxburghii Rroxscaffold_2G00101930
rosa_rugosa Rorug02G0380900
rosa_samantha Rh2AG433800 Rh2BG441800 Rh2CG419500 Rh2DG451900
rosa_wichuraiana Rw2G035370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1152
AatII GACGTC 1 cut(s) 384
AccBSI CCGCTC 1 cut(s) 209
AciI CCGC 2 cut(s) 5, 207
AclWI GGATC 2 cut(s) 167, 1291
AcsI RAATTY 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 381
AdeI CACNNNGTG 1 cut(s) 809
AfaI GTAC 2 cut(s) 1163, 1202
AfiI CCNNNNNNNGG 3 cut(s) 583, 827, 846
AflII CTTAAG 1 cut(s) 1177
AflIII ACRYGT 1 cut(s) 1203
AgsI TTSAA 8 cut(s) 407, 415, 518, 686, 788, 968, 1033, 1315
AjiI CACGTC 1 cut(s) 1206
AjnI CCWGG 1 cut(s) 657
AleI CACNNNNGTG 1 cut(s) 35
AluBI AGCT 8 cut(s) 611, 679, 791, 835, 944, 1058, 1148, 1190
AluI AGCT 8 cut(s) 611, 679, 791, 835, 944, 1058, 1148, 1190
Alw26I GTCTC 3 cut(s) 166, 878, 1212
AlwI GGATC 2 cut(s) 167, 1291
AoxI GGCC 3 cut(s) 256, 499, 952
ApeKI GCWGC 7 cut(s) 347, 486, 611, 800, 941, 1058, 1136
ApoI RAATTY 1 cut(s) 843
Asp700I GAANNNNTTC 1 cut(s) 522
AsuHPI GGTGA 6 cut(s) 19, 22, 114, 196, 707, 821
BbsI GAAGAC 2 cut(s) 67, 1256
BbvI GCAGC 7 cut(s) 334, 498, 598, 812, 953, 1045, 1148
BccI CCATC 7 cut(s) 223, 281, 566, 920, 944, 952, 1123
BceAI ACGGC 2 cut(s) 50, 433
BcgI CGANNNNNNTGC 2 cut(s) 228, 262
BciT130I CCWGG 1 cut(s) 659
BciVI GTATCC 1 cut(s) 603
BcoDI GTCTC 3 cut(s) 166, 878, 1212
BfaI CTAG 1 cut(s) 1008
BfmI CTRYAG 1 cut(s) 745
BfrI CTTAAG 1 cut(s) 1177
BfuI GTATCC 1 cut(s) 603
BisI GCNGC 7 cut(s) 348, 487, 612, 801, 942, 1059, 1137
BlsI GCNGC 7 cut(s) 349, 488, 613, 802, 943, 1060, 1138
Bme1390I CCNGG 1 cut(s) 659
BmgBI CACGTC 1 cut(s) 1206
BmrFI CCNGG 1 cut(s) 659
BmrI ACTGGG 1 cut(s) 837
BmsI GCATC 1 cut(s) 1224
BmuI ACTGGG 1 cut(s) 837
BpiI GAAGAC 2 cut(s) 67, 1256
BpmI CTGGAG 1 cut(s) 680
BpuEI CTTGAG 1 cut(s) 1194
BsaBI GATNNNNATC 2 cut(s) 591, 976
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 657, 821
Bsc4I CCNNNNNNNGG 3 cut(s) 583, 827, 846
Bse1I ACTGG 1 cut(s) 832
Bse3DI GCAATG 2 cut(s) 576, 1146
Bse8I GATNNNNATC 2 cut(s) 591, 976
BseBI CCWGG 1 cut(s) 659
BseDI CCNNGG 2 cut(s) 657, 821
BseGI GGATG 3 cut(s) 57, 912, 955
BseJI GATNNNNATC 2 cut(s) 591, 976
BseLI CCNNNNNNNGG 3 cut(s) 583, 827, 846
BseMI GCAATG 2 cut(s) 576, 1146
BseMII CTCAG 2 cut(s) 611, 666
BseNI ACTGG 1 cut(s) 832
BseXI GCAGC 7 cut(s) 334, 498, 598, 812, 953, 1045, 1148
BseYI CCCAGC 1 cut(s) 944
BshFI GGCC 3 cut(s) 258, 501, 954
BslI CCNNNNNNNGG 3 cut(s) 583, 827, 846
BsmAI GTCTC 3 cut(s) 166, 878, 1212
BsmBI CGTCTC 1 cut(s) 1212
BsmI GAATGC 1 cut(s) 890
BsnI GGCC 3 cut(s) 258, 501, 954
Bso31I GGTCTC 1 cut(s) 166
Bsp1407I TGTACA 1 cut(s) 1161
Bsp143I GATC 2 cut(s) 159, 1283
BspACI CCGC 2 cut(s) 5, 207
BspANI GGCC 3 cut(s) 258, 501, 954
BspCNI CTCAG 2 cut(s) 610, 667
BspPI GGATC 2 cut(s) 167, 1291
BspQI GCTCTTC 1 cut(s) 1180
BspTI CTTAAG 1 cut(s) 1177
BspTNI GGTCTC 1 cut(s) 166
BsrBI CCGCTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 576, 1146
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 1 cut(s) 832
BssECI CCNNGG 2 cut(s) 657, 821
BssMI GATC 2 cut(s) 159, 1283
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 821
Bst2UI CCWGG 1 cut(s) 659
Bst4CI ACNGT 5 cut(s) 34, 411, 635, 749, 1088
Bst6I CTCTTC 3 cut(s) 268, 1180, 1273
BstACI GRCGYC 1 cut(s) 381
BstAFI CTTAAG 1 cut(s) 1177
BstAPI GCANNNNNTGC 1 cut(s) 611
BstAUI TGTACA 1 cut(s) 1161
BstC8I GCNNGC 4 cut(s) 837, 1040, 1146, 1239
BstDEI CTNAG 3 cut(s) 597, 675, 1016
BstF5I GGATG 3 cut(s) 57, 912, 955
BstKTI GATC 2 cut(s) 162, 1286
BstMAI GTCTC 3 cut(s) 166, 878, 1212
BstMBI GATC 2 cut(s) 159, 1283
BstMWI GCNNNNNNNGC 4 cut(s) 255, 611, 797, 1145
BstNI CCWGG 1 cut(s) 659
BstNSI RCATGY 2 cut(s) 630, 1042
BstSCI CCNGG 1 cut(s) 657
BstSFI CTRYAG 1 cut(s) 745
BstV1I GCAGC 7 cut(s) 334, 498, 598, 812, 953, 1045, 1148
BstV2I GAAGAC 2 cut(s) 67, 1256
BstX2I RGATCY 1 cut(s) 1283
BstYI RGATCY 1 cut(s) 1283
BsuI GTATCC 1 cut(s) 603
BsuRI GGCC 3 cut(s) 258, 501, 954
BtrI CACGTC 1 cut(s) 1206
BtsCI GGATG 3 cut(s) 57, 912, 955
BtsIMutI CAGTG 3 cut(s) 21, 640, 1093
Cac8I GCNNGC 4 cut(s) 837, 1040, 1146, 1239
Csp6I GTAC 2 cut(s) 1162, 1201
CviAII CATG 5 cut(s) 449, 627, 1039, 1043, 1273
CviQI GTAC 2 cut(s) 1162, 1201
DdeI CTNAG 3 cut(s) 597, 675, 1016
DpnI GATC 2 cut(s) 161, 1285
DpnII GATC 2 cut(s) 159, 1283
DraI TTTAAA 1 cut(s) 312
DraIII CACNNNGTG 1 cut(s) 809
Eam1104I CTCTTC 3 cut(s) 268, 1180, 1273
EarI CTCTTC 3 cut(s) 268, 1180, 1273
Eco130I CCWWGG 1 cut(s) 821
Eco31I GGTCTC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 657
EcoT14I CCWWGG 1 cut(s) 821
EcoT22I ATGCAT 1 cut(s) 1044
ErhI CCWWGG 1 cut(s) 821
Esp3I CGTCTC 1 cut(s) 1212
FaeI CATG 5 cut(s) 452, 630, 1042, 1046, 1276
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FatI CATG 5 cut(s) 448, 626, 1038, 1042, 1272
Fnu4HI GCNGC 7 cut(s) 348, 487, 612, 801, 942, 1059, 1137
FokI GGATG 3 cut(s) 44, 899, 962
Fsp4HI GCNGC 7 cut(s) 348, 487, 612, 801, 942, 1059, 1137
FspBI CTAG 1 cut(s) 1008
GluI GCNGC 7 cut(s) 348, 487, 612, 801, 942, 1059, 1137
GsaI CCCAGC 1 cut(s) 948
GsuI CTGGAG 1 cut(s) 680
HaeIII GGCC 3 cut(s) 258, 501, 954
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 5 cut(s) 452, 630, 1042, 1046, 1276
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 5 cut(s) 231, 362, 1091, 1168, 1252
HphI GGTGA 6 cut(s) 19, 22, 114, 196, 707, 821
Hpy166II GTNNAC 4 cut(s) 30, 394, 985, 1203
Hpy188I TCNGA 3 cut(s) 267, 428, 676
Hpy188III TCNNGA 2 cut(s) 649, 683
Hpy8I GTNNAC 4 cut(s) 30, 394, 985, 1203
HpyAV CCTTC 6 cut(s) 481, 576, 661, 728, 962, 1219
HpyCH4III ACNGT 5 cut(s) 34, 411, 635, 749, 1088
HpyCH4IV ACGT 3 cut(s) 381, 493, 1205
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 611, 797, 1145
HpyF3I CTNAG 3 cut(s) 597, 675, 1016
HpySE526I ACGT 3 cut(s) 381, 493, 1205
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 5 cut(s) 452, 630, 1042, 1046, 1276
Kzo9I GATC 2 cut(s) 159, 1283
LguI GCTCTTC 1 cut(s) 1180
LmnI GCTCC 1 cut(s) 832
LpnPI CCDG 9 cut(s) 579, 644, 671, 744, 789, 813, 930, 968, 1308
Lsp1109I GCAGC 7 cut(s) 334, 498, 598, 812, 953, 1045, 1148
LweI GCATC 1 cut(s) 1224
MaeI CTAG 1 cut(s) 1008
MaeII ACGT 3 cut(s) 381, 493, 1205
MaeIII GTNAC 5 cut(s) 43, 441, 629, 708, 997
MalI GATC 2 cut(s) 161, 1285
MbiI CCGCTC 1 cut(s) 209
MboI GATC 2 cut(s) 159, 1283
MboII GAAGA 7 cut(s) 67, 285, 518, 676, 1197, 1261, 1290
MflI RGATCY 1 cut(s) 1283
MluCI AATT 6 cut(s) 134, 503, 843, 963, 1229, 1316
MlyI GAGTC 2 cut(s) 1162, 1246
Mph1103I ATGCAT 1 cut(s) 1044
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 6 cut(s) 261, 311, 795, 974, 1083, 1178
MslI CAYNNNNRTG 3 cut(s) 35, 239, 1260
MspA1I CMGCKG 1 cut(s) 944
MspCI CTTAAG 1 cut(s) 1177
MspR9I CCNGG 1 cut(s) 659
Mva1269I GAATGC 1 cut(s) 890
MvaI CCWGG 1 cut(s) 659
MwoI GCNNNNNNNGC 4 cut(s) 255, 611, 797, 1145
NdeII GATC 2 cut(s) 159, 1283
NlaIII CATG 5 cut(s) 452, 630, 1042, 1046, 1276
NmuCI GTSAC 2 cut(s) 43, 708
NsiI ATGCAT 1 cut(s) 1044
NspI RCATGY 2 cut(s) 630, 1042
OliI CACNNNNGTG 1 cut(s) 35
PaeI GCATGC 1 cut(s) 1042
PciSI GCTCTTC 1 cut(s) 1180
PctI GAATGC 1 cut(s) 890
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 3 cut(s) 231, 362, 1091
PkrI GCNGC 7 cut(s) 349, 488, 613, 802, 943, 1060, 1138
PleI GAGTC 2 cut(s) 1162, 1246
PpsI GAGTC 2 cut(s) 1162, 1246
PsiI TTATAA 1 cut(s) 1152
Psp6I CCWGG 1 cut(s) 657
PspFI CCCAGC 1 cut(s) 944
PspGI CCWGG 1 cut(s) 657
PsuI RGATCY 1 cut(s) 1283
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 2 cut(s) 1163, 1202
RsaNI GTAC 2 cut(s) 1162, 1201
RseI CAYNNNNRTG 3 cut(s) 35, 239, 1260
SapI GCTCTTC 1 cut(s) 1180
SaqAI TTAA 6 cut(s) 261, 311, 795, 974, 1083, 1178
SatI GCNGC 7 cut(s) 348, 487, 612, 801, 942, 1059, 1137
Sau3AI GATC 2 cut(s) 159, 1283
SchI GAGTC 2 cut(s) 1162, 1246
ScrFI CCNGG 1 cut(s) 659
SfaNI GCATC 1 cut(s) 1224
SfcI CTRYAG 1 cut(s) 745
SmiMI CAYNNNNRTG 3 cut(s) 35, 239, 1260
SmlI CTYRAG 2 cut(s) 1177, 1209
SmoI CTYRAG 2 cut(s) 1177, 1209
SphI GCATGC 1 cut(s) 1042
Sse9I AATT 6 cut(s) 134, 503, 843, 963, 1229, 1316
SsiI CCGC 2 cut(s) 5, 207
SspMI CTAG 1 cut(s) 1008
StyD4I CCNGG 1 cut(s) 657
StyI CCWWGG 1 cut(s) 821
TaaI ACNGT 5 cut(s) 34, 411, 635, 749, 1088
TaiI ACGT 3 cut(s) 384, 496, 1208
TaqI TCGA 1 cut(s) 238
TasI AATT 6 cut(s) 134, 503, 843, 963, 1229, 1316
TatI WGTACW 2 cut(s) 1161, 1200
TfiI GAWTC 3 cut(s) 231, 362, 1091
Tru1I TTAA 6 cut(s) 261, 311, 795, 974, 1083, 1178
Tru9I TTAA 6 cut(s) 261, 311, 795, 974, 1083, 1178
TscAI CASTG 3 cut(s) 21, 640, 1093
TseFI GTSAC 2 cut(s) 43, 708
TseI GCWGC 7 cut(s) 347, 486, 611, 800, 941, 1058, 1136
Tsp45I GTSAC 2 cut(s) 43, 708
TspDTI ATGAA 5 cut(s) 223, 375, 391, 883, 1083
TspGWI ACGGA 2 cut(s) 464, 519
TspRI CASTG 3 cut(s) 21, 640, 1093
Vha464I CTTAAG 1 cut(s) 1177
XapI RAATTY 1 cut(s) 843
XceI RCATGY 2 cut(s) 630, 1042
XcmI CCANNNNNNNNNTGG 1 cut(s) 1060
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 1 cut(s) 1008
ZraI GACGTC 1 cut(s) 382
Zsp2I ATGCAT 1 cut(s) 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.