FvH4_6g33800

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006298
Physical Location & Seq
Forward (+)
26778183 .. 26780779
2597 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g33800.t1

Sequence Viewer

Length: 444 bp
ATGGCGTCCAAAACCCTAATTCGAACCGGAGCTTCTCTGATGAACCGGTACCTATCAAAGCCACTGCTCCACCCAAACCCGAACCCGAATTCGACTCACCAGGTTGTATCCCACGCCCTCGAAATCACTCCCAAGCTGTTCCCCTCCCTCTCCAACTTCGACATCTCCCTCCGCTTTCCCCAAAACGACGCCGAATCGATCACCAGGGTCTTCAACGAGGGCTTCGTCCACCCTTGCGGCCTCCCTTATCTCCCGCTCATATTACCTGAAGGAGACGAGTCTTCCTCTAATGAGCCGATGATATTATTTCCTAAGCGGACATTCCAGCCTAGTACTATCAGGCGTAAGAGGAACCATGGATTTTTTGCTCGCAAGGCAACCAAGGGTGGACGGAGAGTTATTGCTCGAAGAATAGCAAAGGGTCGAGCAAGAATTACAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

148

Amino Acids

16.53

Weight (kDa)

11.3

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 105 - 147 2.2e-18 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 48
AccB1I GGYRCC 1 cut(s) 48
AccBSI CCGCTC 1 cut(s) 256
AciI CCGC 4 cut(s) 172, 237, 254, 316
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 288
AcyI GRCGYC 2 cut(s) 5, 189
AfaI GTAC 2 cut(s) 50, 334
AgeI ACCGGT 1 cut(s) 45
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 2 cut(s) 99, 203
AluBI AGCT 3 cut(s) 32, 136, 440
AluI AGCT 3 cut(s) 32, 136, 440
Alw26I GTCTC 1 cut(s) 267
AoxI GGCC 1 cut(s) 238
ApoI RAATTY 1 cut(s) 88
AsiGI ACCGGT 1 cut(s) 45
Asp718I GGTACC 1 cut(s) 48
AsuHPI GGTGA 2 cut(s) 89, 193
AsuII TTCGAA 1 cut(s) 22
BanI GGYRCC 1 cut(s) 48
BbsI GAAGAC 2 cut(s) 202, 273
BciT130I CCWGG 2 cut(s) 101, 205
BciVI GTATCC 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 1 cut(s) 330
BfuI GTATCC 1 cut(s) 118
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
BmcAI AGTACT 1 cut(s) 334
Bme1390I CCNGG 2 cut(s) 101, 205
BmiI GGNNCC 2 cut(s) 50, 353
BmrFI CCNGG 2 cut(s) 101, 205
BpiI GAAGAC 2 cut(s) 202, 273
BplI GAGNNNNNCTC 2 cut(s) 269, 301
Bpu10I CCTNAGC 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 197
BsaHI GRCGYC 2 cut(s) 5, 189
BsaJI CCNNGG 3 cut(s) 204, 355, 381
BsaWI WCCGGW 2 cut(s) 26, 45
Bse118I RCCGGY 1 cut(s) 45
BseBI CCWGG 2 cut(s) 101, 205
BseCI ATCGAT 1 cut(s) 197
BseDI CCNNGG 3 cut(s) 204, 355, 381
BshFI GGCC 1 cut(s) 240
BshNI GGYRCC 1 cut(s) 48
BshTI ACCGGT 1 cut(s) 45
BshVI ATCGAT 1 cut(s) 197
BsiSI CCGG 2 cut(s) 27, 46
BsmAI GTCTC 1 cut(s) 267
BsmBI CGTCTC 1 cut(s) 267
BsnI GGCC 1 cut(s) 240
Bsp119I TTCGAA 1 cut(s) 22
Bsp143I GATC 1 cut(s) 198
Bsp19I CCATGG 1 cut(s) 355
BspACI CCGC 4 cut(s) 172, 237, 254, 316
BspANI GGCC 1 cut(s) 240
BspDI ATCGAT 1 cut(s) 197
BspLI GGNNCC 2 cut(s) 50, 353
BspT104I TTCGAA 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 48
BsrBI CCGCTC 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 45
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 3 cut(s) 204, 355, 381
BssMI GATC 1 cut(s) 198
BssNI GRCGYC 2 cut(s) 5, 189
BssT1I CCWWGG 2 cut(s) 355, 381
Bst2UI CCWGG 2 cut(s) 101, 205
BstACI GRCGYC 2 cut(s) 5, 189
BstBI TTCGAA 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 2 cut(s) 312, 441
BstDSI CCRYGG 1 cut(s) 355
BstKTI GATC 1 cut(s) 201
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 1 cut(s) 198
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNI CCWGG 2 cut(s) 101, 205
BstSCI CCNGG 2 cut(s) 99, 203
BstV2I GAAGAC 2 cut(s) 202, 273
Bsu15I ATCGAT 1 cut(s) 197
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 1 cut(s) 240
BsuTUI ATCGAT 1 cut(s) 197
BtgI CCRYGG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 1 cut(s) 62
Cac8I GCNNGC 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 45
ClaI ATCGAT 1 cut(s) 197
CseI GACGC 1 cut(s) 197
CsiI ACCWGGT 1 cut(s) 99
Csp6I GTAC 2 cut(s) 49, 333
CspAI ACCGGT 1 cut(s) 45
CviAII CATG 1 cut(s) 356
CviJI RGCY 8 cut(s) 32, 61, 136, 222, 240, 295, 328, 440
CviKI_1 RGCY 8 cut(s) 32, 61, 136, 222, 240, 295, 328, 440
CviQI GTAC 2 cut(s) 49, 333
DdeI CTNAG 2 cut(s) 312, 441
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
Eco130I CCWWGG 2 cut(s) 355, 381
Eco57I CTGAAG 1 cut(s) 288
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 2 cut(s) 99, 203
EcoT14I CCWWGG 2 cut(s) 355, 381
ErhI CCWWGG 2 cut(s) 355, 381
Esp3I CGTCTC 1 cut(s) 267
FaeI CATG 1 cut(s) 359
FaiI YATR 2 cut(s) 260, 357
FatI CATG 1 cut(s) 355
FauI CCCGC 1 cut(s) 261
Fnu4HI GCNGC 1 cut(s) 238
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 1 cut(s) 330
GluI GCNGC 1 cut(s) 238
HaeIII GGCC 1 cut(s) 240
HapII CCGG 2 cut(s) 27, 46
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 2 cut(s) 5, 189
Hin1II CATG 1 cut(s) 359
HinfI GANTC 3 cut(s) 94, 194, 278
HpaII CCGG 2 cut(s) 27, 46
HphI GGTGA 2 cut(s) 89, 193
Hpy166II GTNNAC 2 cut(s) 229, 389
Hpy188I TCNGA 1 cut(s) 39
Hpy8I GTNNAC 2 cut(s) 229, 389
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 2 cut(s) 312, 441
Hsp92I GRCGYC 2 cut(s) 5, 189
Hsp92II CATG 1 cut(s) 359
KpnI GGTACC 1 cut(s) 52
Kzo9I GATC 1 cut(s) 198
LmnI GCTCC 2 cut(s) 29, 72
LpnPI CCDG 9 cut(s) 40, 59, 86, 113, 190, 217, 279, 325, 338
MabI ACCWGGT 1 cut(s) 99
MaeI CTAG 1 cut(s) 330
MalI GATC 1 cut(s) 200
MbiI CCGCTC 1 cut(s) 256
MboI GATC 1 cut(s) 198
MboII GAAGA 3 cut(s) 202, 273, 420
MluCI AATT 3 cut(s) 18, 88, 432
MlyI GAGTC 2 cut(s) 88, 287
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 8 cut(s) 128, 154, 158, 179, 211, 251, 295, 342
MspI CCGG 2 cut(s) 27, 46
MspR9I CCNGG 2 cut(s) 101, 205
MvaI CCWGG 2 cut(s) 101, 205
MwoI GCNNNNNNNGC 1 cut(s) 374
NcoI CCATGG 1 cut(s) 355
NdeII GATC 1 cut(s) 198
NlaIII CATG 1 cut(s) 359
NlaIV GGNNCC 2 cut(s) 50, 353
NspV TTCGAA 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 1 cut(s) 194
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 1 cut(s) 239
PleI GAGTC 2 cut(s) 88, 286
PpsI GAGTC 2 cut(s) 88, 286
Psp6I CCWGG 2 cut(s) 99, 203
PspGI CCWGG 2 cut(s) 99, 203
PspN4I GGNNCC 2 cut(s) 50, 353
RsaI GTAC 2 cut(s) 50, 334
RsaNI GTAC 2 cut(s) 49, 333
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 1 cut(s) 198
ScaI AGTACT 1 cut(s) 334
SchI GAGTC 2 cut(s) 88, 287
ScrFI CCNGG 2 cut(s) 101, 205
SetI ASST 6 cut(s) 34, 54, 105, 138, 268, 442
SexAI ACCWGGT 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 22
Sse9I AATT 3 cut(s) 18, 88, 432
SsiI CCGC 4 cut(s) 172, 237, 254, 316
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 99, 203
StyI CCWWGG 2 cut(s) 355, 381
TaqI TCGA 7 cut(s) 22, 92, 120, 159, 197, 406, 424
TasI AATT 3 cut(s) 18, 88, 432
TatI WGTACW 1 cut(s) 332
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 1 cut(s) 194
TscAI CASTG 1 cut(s) 69
TspDTI ATGAA 1 cut(s) 56
TspGWI ACGGA 1 cut(s) 406
TspRI CASTG 1 cut(s) 69
XapI RAATTY 1 cut(s) 88
XspI CTAG 1 cut(s) 330
ZrmI AGTACT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.