MD17G1173900.v1.1

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006298
Physical Location & Seq
Reverse (-)
18090933 .. 18093635
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1173900.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGCGTCCAAAACCCTAATTCGAACCGGAGCTTCCCTCATAAACCGGTTCCTATCCAAGCCATTGCTCCACCCGAACCCGAGTCCGAATCGCCATATTGTCTCTCACGGCCTCGAAATCACTCCCAAGATGTTCCCGTCTCTCTCCCACTTCGACACCTCCCTCCGTTTGCCCCAAAACGACGCCGAATCGCTTAGACGGGTCTCCTCTGAAGGGTTCTTGTACCCCTGCGGCCTCCCTTATCTCCCGTTCTACTTACCTGACGGTGATTCTTCATCAACAGAGCCAATGCTGTTATTTCCAAAGCGAACGTTCCAGCCCAGTACCATCCGGCGGAAGAGGAACCATGGATTTTTTGCTCGCAAGGCAACCAAGGGTGGACGGAGAGTAATTGCTAGAAGAATAGCGAAAGGTCGAGCAAGAGTTACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

145

Amino Acids

16.23

Weight (kDa)

11.53

Isoelectric Point (pI)

69.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 102 - 144 4.1e-18 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 231, 334
AclI AACGTT 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 2 cut(s) 5, 183
AfaI GTAC 2 cut(s) 224, 325
AfiI CCNNNNNNNGG 2 cut(s) 213, 333
AgeI ACCGGT 1 cut(s) 45
AluBI AGCT 2 cut(s) 32, 431
AluI AGCT 2 cut(s) 32, 431
Alw26I GTCTC 3 cut(s) 106, 144, 208
Ama87I CYCGRG 1 cut(s) 79
AoxI GGCC 2 cut(s) 109, 232
AsiGI ACCGGT 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 278
AsuII TTCGAA 1 cut(s) 22
AvaI CYCGRG 1 cut(s) 79
BccI CCATC 1 cut(s) 335
BceAI ACGGC 1 cut(s) 124
BcoDI GTCTC 3 cut(s) 106, 144, 208
BfaI CTAG 1 cut(s) 396
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
BmeT110I CYCGRG 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 50, 344
BmrI ACTGGG 1 cut(s) 315
BmuI ACTGGG 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 21, 53
Bpu14I TTCGAA 1 cut(s) 22
BsaHI GRCGYC 2 cut(s) 5, 183
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 2 cut(s) 346, 372
BsaWI WCCGGW 2 cut(s) 26, 45
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 333
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 62
BseDI CCNNGG 2 cut(s) 346, 372
BseGI GGATG 1 cut(s) 327
BseLI CCNNNNNNNGG 2 cut(s) 213, 333
BseMI GCAATG 1 cut(s) 62
BseNI ACTGG 1 cut(s) 321
BseRI GAGGAG 1 cut(s) 196
BshFI GGCC 2 cut(s) 111, 234
BshTI ACCGGT 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 3 cut(s) 27, 46, 331
BslI CCNNNNNNNGG 2 cut(s) 213, 333
BsmAI GTCTC 3 cut(s) 106, 144, 208
BsmBI CGTCTC 1 cut(s) 144
BsnI GGCC 2 cut(s) 111, 234
Bso31I GGTCTC 1 cut(s) 208
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 22
Bsp19I CCATGG 1 cut(s) 346
BspACI CCGC 2 cut(s) 231, 334
BspANI GGCC 2 cut(s) 111, 234
BspLI GGNNCC 2 cut(s) 50, 344
BspT104I TTCGAA 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 45
BsrI ACTGG 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 346, 372
BssNI GRCGYC 2 cut(s) 5, 183
BssT1I CCWWGG 2 cut(s) 346, 372
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 1 cut(s) 332
BstACI GRCGYC 2 cut(s) 5, 183
BstBI TTCGAA 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 1 cut(s) 346
BstF5I GGATG 1 cut(s) 327
BstMAI GTCTC 3 cut(s) 106, 144, 208
BstMWI GCNNNNNNNGC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 111, 234
BtgI CCRYGG 1 cut(s) 346
BtsCI GGATG 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 45
CseI GACGC 1 cut(s) 191
Csp6I GTAC 2 cut(s) 223, 324
CspAI ACCGGT 1 cut(s) 45
CviAII CATG 1 cut(s) 347
CviJI RGCY 7 cut(s) 32, 61, 111, 234, 286, 319, 431
CviKI_1 RGCY 7 cut(s) 32, 61, 111, 234, 286, 319, 431
CviQI GTAC 2 cut(s) 223, 324
DdeI CTNAG 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
EciI GGCGGA 1 cut(s) 349
Eco130I CCWWGG 2 cut(s) 346, 372
Eco31I GGTCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 79
EcoT14I CCWWGG 2 cut(s) 346, 372
ErhI CCWWGG 2 cut(s) 346, 372
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 1 cut(s) 350
FaiI YATR 3 cut(s) 41, 96, 348
FatI CATG 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 232
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 1 cut(s) 396
GluI GCNGC 1 cut(s) 232
HaeIII GGCC 2 cut(s) 111, 234
HapII CCGG 3 cut(s) 27, 46, 331
HgaI GACGC 1 cut(s) 191
Hin1I GRCGYC 2 cut(s) 5, 183
Hin1II CATG 1 cut(s) 350
HinfI GANTC 4 cut(s) 82, 88, 188, 269
HpaII CCGG 3 cut(s) 27, 46, 331
HphI GGTGA 1 cut(s) 278
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 2 cut(s) 87, 211
Hpy8I GTNNAC 1 cut(s) 380
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4IV ACGT 1 cut(s) 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 2 cut(s) 5, 183
Hsp92II CATG 1 cut(s) 350
LmnI GCTCC 2 cut(s) 29, 72
LpnPI CCDG 7 cut(s) 40, 59, 241, 273, 329, 334, 344
MaeI CTAG 1 cut(s) 396
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 424
MboII GAAGA 3 cut(s) 264, 349, 411
MluCI AATT 2 cut(s) 18, 390
MlyI GAGTC 1 cut(s) 91
MnlI CCTC 7 cut(s) 47, 122, 169, 173, 217, 245, 333
MseI TTAA 1 cut(s) 433
MspI CCGG 3 cut(s) 27, 46, 331
MwoI GCNNNNNNNGC 1 cut(s) 365
NcoI CCATGG 1 cut(s) 346
NlaIII CATG 1 cut(s) 350
NlaIV GGNNCC 2 cut(s) 50, 344
NspV TTCGAA 1 cut(s) 22
PfeI GAWTC 3 cut(s) 88, 188, 269
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 1 cut(s) 233
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp1406I AACGTT 1 cut(s) 311
PspN4I GGNNCC 2 cut(s) 50, 344
RsaI GTAC 2 cut(s) 224, 325
RsaNI GTAC 2 cut(s) 223, 324
SaqAI TTAA 1 cut(s) 433
SatI GCNGC 1 cut(s) 232
SchI GAGTC 1 cut(s) 91
SetI ASST 6 cut(s) 34, 161, 262, 314, 415, 433
SfuI TTCGAA 1 cut(s) 22
Sse9I AATT 2 cut(s) 18, 390
SsiI CCGC 2 cut(s) 231, 334
SspMI CTAG 1 cut(s) 396
StyI CCWWGG 2 cut(s) 346, 372
TaaI ACNGT 1 cut(s) 266
TaiI ACGT 1 cut(s) 314
TaqI TCGA 4 cut(s) 22, 114, 153, 415
TasI AATT 2 cut(s) 18, 390
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 3 cut(s) 88, 188, 269
Tru1I TTAA 1 cut(s) 433
Tru9I TTAA 1 cut(s) 433
TspDTI ATGAA 1 cut(s) 264
TspGWI ACGGA 2 cut(s) 155, 397
XspI CTAG 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.