Rh2DG454600

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006298
Physical Location & Seq
Forward (+)
65792600 .. 65795379
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG454600.1

Sequence Viewer

Length: 447 bp
ATGGCGTCCAAAACCCTAATTCGAACCGGAGCTTCTCTGATGAACCGGTTCCTATCAAAACCACTGCTCCACCCGAACCCGAATTCGACTCACCAGAACCAGATTGTGTCCCACGCCCTCGAAATCACTCCCAAGCTATTCCCCTCTCTCTCCAACTTCGACACCTCCCTCCGTTTTCCCCAAAACGACGCCGCATCGATCACAAGGGTCTTCAATGAGGGCTTCGTGCACCCTTGCGGCCTCCCTTATCTCCCGCTCATCTTACCCGAAGGAGATGACTCTTCATCTAATGAGCCAATGATATTATTTCCGAAGCGGACATTCCAGCCTAGTACTATCAGGCGTAAGAGGAACCATGGCTTTTTTGCTCGGTATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCACACACACACCATCTGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

148

Amino Acids

16.52

Weight (kDa)

9.89

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 105 - 125 1.1e-07 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 256
AciI CCGC 4 cut(s) 192, 237, 254, 316
AcsI RAATTY 1 cut(s) 82
AcyI GRCGYC 2 cut(s) 5, 189
AfaI GTAC 1 cut(s) 334
AgeI ACCGGT 1 cut(s) 45
AgsI TTSAA 1 cut(s) 214
AluBI AGCT 2 cut(s) 32, 136
AluI AGCT 2 cut(s) 32, 136
Alw21I GWGCWC 1 cut(s) 231
Alw44I GTGCAC 1 cut(s) 227
AoxI GGCC 1 cut(s) 238
ApaLI GTGCAC 1 cut(s) 227
ApoI RAATTY 1 cut(s) 82
AsiGI ACCGGT 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 83
AsuII TTCGAA 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 1 cut(s) 442
BfaI CTAG 1 cut(s) 330
BisI GCNGC 2 cut(s) 192, 238
BlsI GCNGC 2 cut(s) 193, 239
BmcAI AGTACT 1 cut(s) 334
BmiI GGNNCC 2 cut(s) 50, 353
BmsI GCATC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 202
Bpu14I TTCGAA 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 197
BsaHI GRCGYC 2 cut(s) 5, 189
BsaJI CCNNGG 1 cut(s) 355
BsaWI WCCGGW 2 cut(s) 26, 45
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bse118I RCCGGY 1 cut(s) 45
BseCI ATCGAT 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 231
BshFI GGCC 1 cut(s) 240
BshTI ACCGGT 1 cut(s) 45
BshVI ATCGAT 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 2 cut(s) 27, 46
BslFI GGGAC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 94
BsnI GGCC 1 cut(s) 240
Bsp119I TTCGAA 1 cut(s) 22
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 1 cut(s) 198
Bsp19I CCATGG 1 cut(s) 355
BspACI CCGC 4 cut(s) 192, 237, 254, 316
BspANI GGCC 1 cut(s) 240
BspDI ATCGAT 1 cut(s) 197
BspLI GGNNCC 2 cut(s) 50, 353
BspT104I TTCGAA 1 cut(s) 22
BsrBI CCGCTC 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 45
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 1 cut(s) 198
BssNI GRCGYC 2 cut(s) 5, 189
BssT1I CCWWGG 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 286
BstACI GRCGYC 2 cut(s) 5, 189
BstBI TTCGAA 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 355
BstKTI GATC 1 cut(s) 201
BstMBI GATC 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 231
BstV2I GAAGAC 1 cut(s) 202
Bsu15I ATCGAT 1 cut(s) 197
BsuRI GGCC 1 cut(s) 240
BsuTUI ATCGAT 1 cut(s) 197
BtgI CCRYGG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 45
ClaI ATCGAT 1 cut(s) 197
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 333
CspAI ACCGGT 1 cut(s) 45
CviAII CATG 1 cut(s) 356
CviJI RGCY 7 cut(s) 32, 136, 222, 240, 295, 328, 360
CviKI_1 RGCY 7 cut(s) 32, 136, 222, 240, 295, 328, 360
CviQI GTAC 1 cut(s) 333
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 355
EcoRI GAATTC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 355
FaeI CATG 1 cut(s) 359
FaiI YATR 2 cut(s) 357, 443
FaqI GGGAC 1 cut(s) 94
FatI CATG 1 cut(s) 355
FauI CCCGC 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 192, 238
Fsp4HI GCNGC 2 cut(s) 192, 238
FspBI CTAG 1 cut(s) 330
GluI GCNGC 2 cut(s) 192, 238
HaeIII GGCC 1 cut(s) 240
HapII CCGG 2 cut(s) 27, 46
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 2 cut(s) 5, 189
Hin1II CATG 1 cut(s) 359
HinfI GANTC 2 cut(s) 88, 278
HpaII CCGG 2 cut(s) 27, 46
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 39, 312, 439
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 263
HpyCH4V TGCA 1 cut(s) 229
Hsp92I GRCGYC 2 cut(s) 5, 189
Hsp92II CATG 1 cut(s) 359
Kzo9I GATC 1 cut(s) 198
LmnI GCTCC 2 cut(s) 29, 72
LpnPI CCDG 6 cut(s) 40, 59, 107, 113, 325, 338
LweI GCATC 1 cut(s) 203
MaeI CTAG 1 cut(s) 330
MalI GATC 1 cut(s) 200
MbiI CCGCTC 1 cut(s) 256
MboI GATC 1 cut(s) 198
MboII GAAGA 2 cut(s) 202, 273
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 2 cut(s) 18, 82
MlyI GAGTC 2 cut(s) 82, 272
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 7 cut(s) 128, 154, 175, 179, 211, 251, 342
MroXI GAANNNNTTC 1 cut(s) 47
MspI CCGG 2 cut(s) 27, 46
NcoI CCATGG 1 cut(s) 355
NdeII GATC 1 cut(s) 198
NlaIII CATG 1 cut(s) 359
NlaIV GGNNCC 2 cut(s) 50, 353
NspV TTCGAA 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 47
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 2 cut(s) 193, 239
PleI GAGTC 2 cut(s) 82, 272
PpsI GAGTC 2 cut(s) 82, 272
PspN4I GGNNCC 2 cut(s) 50, 353
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
SatI GCNGC 2 cut(s) 192, 238
Sau3AI GATC 1 cut(s) 198
ScaI AGTACT 1 cut(s) 334
SchI GAGTC 2 cut(s) 82, 272
SduI GDGCHC 1 cut(s) 231
SetI ASST 3 cut(s) 34, 138, 167
SfaNI GCATC 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 22
Sse9I AATT 2 cut(s) 18, 82
SsiI CCGC 4 cut(s) 192, 237, 254, 316
SspMI CTAG 1 cut(s) 330
StyI CCWWGG 1 cut(s) 355
TaqI TCGA 5 cut(s) 22, 86, 120, 159, 197
TasI AATT 2 cut(s) 18, 82
TatI WGTACW 1 cut(s) 332
TauI GCSGC 2 cut(s) 194, 240
TscAI CASTG 1 cut(s) 69
TspDTI ATGAA 2 cut(s) 56, 273
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 1 cut(s) 69
VneI GTGCAC 1 cut(s) 227
XapI RAATTY 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 1 cut(s) 330
ZrmI AGTACT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.