MD09G1191200.v1.1

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006298
Physical Location & Seq
Reverse (-)
16694765 .. 16697085
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1191200.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGCGTCCAAAACCCTAATTCGAACCGGAGCTTCCCTCGTGAACCGGTTCGTATCCAAGCCATTGCTCCACCCGAACCCGAATCCGAATCGCCATATTGTATCTCACGGCCTCGGAATCACTCCCAAGCTGTTCCCGTCTCTCTCCCACTACGACACCTCCCTCCGTTTTCCCCAAAACGATGCCGAATCGCTGAAACGGGTCTCCTCTGAAGGGTTTTGGTACCCCTGCGGCCTCCCTTATCTTCCGTTGTACTTACCTGACGGAGATGAATCTTCATCAACAGAGCCAATGCTATTATTTCCAAAGCGAACCTTCCAGCCTAGTACCATCCGGCGGAAGAGGAACCATGGATTTTTTGCTCGTAAGGCAACCAAGGGTGGACGAAGAGTAATAGCTAGAAGAATAGCAAAGGGTCGAGCAAGGGTCACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

146

Amino Acids

16.33

Weight (kDa)

11.29

Isoelectric Point (pI)

56.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 103 - 145 4.1e-18 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 222
AciI CCGC 2 cut(s) 231, 337
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 224, 254, 328
AfiI CCNNNNNNNGG 2 cut(s) 213, 336
AgeI ACCGGT 1 cut(s) 45
AluBI AGCT 4 cut(s) 32, 130, 398, 434
AluI AGCT 4 cut(s) 32, 130, 398, 434
Alw26I GTCTC 2 cut(s) 144, 208
AoxI GGCC 2 cut(s) 109, 232
AsiGI ACCGGT 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 47
Asp718I GGTACC 1 cut(s) 222
AsuII TTCGAA 1 cut(s) 22
BanI GGYRCC 1 cut(s) 222
BauI CACGAG 1 cut(s) 38
BccI CCATC 1 cut(s) 338
BceAI ACGGC 1 cut(s) 124
BciVI GTATCC 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 144, 208
BfaI CTAG 2 cut(s) 324, 399
BfuI GTATCC 1 cut(s) 64
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
BmiI GGNNCC 2 cut(s) 224, 347
BmsI GCATC 1 cut(s) 172
BplI GAGNNNNNCTC 2 cut(s) 21, 53
Bpu14I TTCGAA 1 cut(s) 22
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 3 cut(s) 112, 349, 375
BsaWI WCCGGW 2 cut(s) 26, 45
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 336
Bse118I RCCGGY 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 62
BseDI CCNNGG 3 cut(s) 112, 349, 375
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 213, 336
BseMI GCAATG 1 cut(s) 62
BseRI GAGGAG 1 cut(s) 196
BshFI GGCC 2 cut(s) 111, 234
BshNI GGYRCC 1 cut(s) 222
BshTI ACCGGT 1 cut(s) 45
BsiSI CCGG 3 cut(s) 27, 46, 334
BslI CCNNNNNNNGG 2 cut(s) 213, 336
BsmAI GTCTC 2 cut(s) 144, 208
BsmBI CGTCTC 1 cut(s) 144
BsnI GGCC 2 cut(s) 111, 234
Bso31I GGTCTC 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 22
Bsp19I CCATGG 1 cut(s) 349
BspACI CCGC 2 cut(s) 231, 337
BspANI GGCC 2 cut(s) 111, 234
BspLI GGNNCC 2 cut(s) 224, 347
BspT104I TTCGAA 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 45
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 3 cut(s) 112, 349, 375
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 349, 375
Bst2BI CACGAG 1 cut(s) 38
Bst6I CTCTTC 2 cut(s) 335, 382
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 349
BstF5I GGATG 1 cut(s) 330
BstMAI GTCTC 2 cut(s) 144, 208
BstMWI GCNNNNNNNGC 1 cut(s) 368
BsuI GTATCC 1 cut(s) 64
BsuRI GGCC 2 cut(s) 111, 234
BtgI CCRYGG 1 cut(s) 349
BtsCI GGATG 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 45
Csp6I GTAC 3 cut(s) 223, 253, 327
CspAI ACCGGT 1 cut(s) 45
CviAII CATG 1 cut(s) 350
CviJI RGCY 9 cut(s) 32, 61, 111, 130, 234, 289, 322, 398, 434
CviKI_1 RGCY 9 cut(s) 32, 61, 111, 130, 234, 289, 322, 398, 434
CviQI GTAC 3 cut(s) 223, 253, 327
Eam1104I CTCTTC 2 cut(s) 335, 382
EarI CTCTTC 2 cut(s) 335, 382
EciI GGCGGA 1 cut(s) 352
Eco130I CCWWGG 2 cut(s) 349, 375
Eco31I GGTCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 231
EcoT14I CCWWGG 2 cut(s) 349, 375
ErhI CCWWGG 2 cut(s) 349, 375
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 1 cut(s) 353
FaiI YATR 2 cut(s) 96, 351
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FatI CATG 1 cut(s) 349
Fnu4HI GCNGC 1 cut(s) 232
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 2 cut(s) 324, 399
GluI GCNGC 1 cut(s) 232
HaeIII GGCC 2 cut(s) 111, 234
HapII CCGG 3 cut(s) 27, 46, 334
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 353
HinfI GANTC 5 cut(s) 82, 88, 117, 188, 272
HpaII CCGG 3 cut(s) 27, 46, 334
Hpy166II GTNNAC 2 cut(s) 43, 383
Hpy188I TCNGA 3 cut(s) 87, 116, 211
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 2 cut(s) 43, 383
HpyAV CCTTC 2 cut(s) 206, 325
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 353
KpnI GGTACC 1 cut(s) 226
LmnI GCTCC 2 cut(s) 29, 72
LpnPI CCDG 6 cut(s) 40, 59, 241, 273, 332, 347
LweI GCATC 1 cut(s) 172
MaeI CTAG 2 cut(s) 324, 399
MaeIII GTNAC 1 cut(s) 427
MboII GAAGA 5 cut(s) 236, 267, 352, 399, 414
MluCI AATT 1 cut(s) 18
MnlI CCTC 7 cut(s) 47, 122, 169, 173, 217, 245, 336
MroXI GAANNNNTTC 1 cut(s) 47
MseI TTAA 1 cut(s) 436
MspI CCGG 3 cut(s) 27, 46, 334
MwoI GCNNNNNNNGC 1 cut(s) 368
NcoI CCATGG 1 cut(s) 349
NlaIII CATG 1 cut(s) 353
NlaIV GGNNCC 2 cut(s) 224, 347
NmuCI GTSAC 1 cut(s) 427
NspV TTCGAA 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 5 cut(s) 82, 88, 117, 188, 272
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 1 cut(s) 233
PspN4I GGNNCC 2 cut(s) 224, 347
RsaI GTAC 3 cut(s) 224, 254, 328
RsaNI GTAC 3 cut(s) 223, 253, 327
SaqAI TTAA 1 cut(s) 436
SatI GCNGC 1 cut(s) 232
SetI ASST 7 cut(s) 34, 132, 161, 262, 317, 400, 436
SfaNI GCATC 1 cut(s) 172
SfuI TTCGAA 1 cut(s) 22
Sse9I AATT 1 cut(s) 18
SsiI CCGC 2 cut(s) 231, 337
SspMI CTAG 2 cut(s) 324, 399
StyI CCWWGG 2 cut(s) 349, 375
TaqI TCGA 2 cut(s) 22, 418
TasI AATT 1 cut(s) 18
TatI WGTACW 1 cut(s) 252
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 5 cut(s) 82, 88, 117, 188, 272
Tru1I TTAA 1 cut(s) 436
Tru9I TTAA 1 cut(s) 436
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 2 cut(s) 267, 285
TspGWI ACGGA 3 cut(s) 155, 237, 279
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 2 cut(s) 324, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.