FvH4_6g38780

Phytoene synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003237
Physical Location & Seq
Forward (+)
30677129 .. 30680328
3200 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38780.t1

Sequence Viewer

Length: 1197 bp
ATGTCTGGTGTTCTTCTTTGGGTGGTGAACCCCAAAGAGAATGCCACCTCTTTGCTTAGTCTGATGCCCAGAATTGGTAGCCCAAAGAGGTCCAAGTTCTGCTCCAGGCTGAGTTTCTCTCCTGGGATTATAGCCTATTCAGGAGCTGTTGCTCACCCTCCAAGGTCTTCAGAAGAGAGGGTCTATGAAGTGGTGCTCAAGCAAGCTGCTTTGGTGAGAGAACAGAGGAGGCCTAAAGAGAAAGCTTTGGACTTTGATCAAAGGATTGAAGGTAGTGATGGTATGACCAACCGGGATTTGCTGAATGAGGCATATGATAGGTGTGGTGAGGTTTGTGCCGAGTATGCCAAGACTTTTTACTTGGGCACATTACTTATGACACCAGAGCGGAGGCGAGCAGTTTGGGCAATTTATGTGTGGTGCAGAAGGACTGATGAACTAGTGGATGGACCTAATGCTTCATATATCACACCTAAAGCTCTTGACAGATGGGAAAAAAGACTAACTGACCTTTTTGAAGGTCGTCCATATGATATGTATGATGCAGCTCTATCTGATACCGTCACCAAGTACCCAGTTGATATACAGCCCTTCAAGGACATGGTAGAAGGAATGAGGTTAGACTTGAGAAAATCAAGATACCAGAACTTTGATGAACTTTACCTCTACTGCTACTATGTTGCTGGAACTGTTGGACTGATGAGTGTTCCAGTAATGGGGATCGCCCCTGAATCAAAGGCTTCGACAGAGACTGTTTACAATGCTGCATTGGCCCTTGGAATTGCTAATCAGCTCACTAACATACTTCGAGATGTTGGAGAAGATGCTAGGAGAGGAAGAATCTATCTTCCACAAGATGAGCTTGCTCAGGCTGGACTATCGGACGACGATATCTTCCGCGGGAAAGTGACAGACAAGTGGCAAAGTTTCATGAAGGGCCAAATAAAGCGAGCTAGGATGTTCTTTGATGAGGCTGAAAAAGGAGTTGCAGAGCTCAACTCAGCAAGTAGATGGCCAGTGTGGGCTTCTTTGTTGCTATATAGGCAGATTCTGGATGCCATTGAAGCAAATGGTTATGATAATTTCACAAAGAGGGCTTATGTAGGAAAAGCAAAGAAGTTAGTATCACTGCCAATAGCTTATGGGAGAGCAATTATAGGTCCTTCTAAATTAACTGAGCAGTTGGTGGCAAGATAA

Protein Analysis

399

Amino Acids

45.1

Weight (kDa)

8.85

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQS_PSY PF00494 114 - 370 1.9e-76 Squalene/phytoene synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 388
AccII CGCG 1 cut(s) 900
AciI CCGC 3 cut(s) 388, 898, 900
AclWI GGATC 1 cut(s) 728
AcoI YGGCCR 1 cut(s) 1013
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 2 cut(s) 74, 716
AgsI TTSAA 4 cut(s) 269, 518, 595, 1064
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 2 cut(s) 104, 121
Alw21I GWGCWC 2 cut(s) 198, 996
Alw26I GTCTC 1 cut(s) 743
AlwI GGATC 1 cut(s) 728
AlwNI CAGNNNCTG 3 cut(s) 146, 752, 1051
AoxI GGCC 4 cut(s) 230, 771, 937, 1013
ApeKI GCWGC 3 cut(s) 206, 545, 764
AspS9I GGNCC 5 cut(s) 90, 449, 772, 937, 1160
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 5 cut(s) 37, 146, 226, 338, 556
AvaII GGWCC 3 cut(s) 90, 449, 1160
BaeGI GKGCMC 1 cut(s) 368
BalI TGGCCA 1 cut(s) 1015
BanII GRGCYC 1 cut(s) 996
BbsI GAAGAC 1 cut(s) 159
Bbv12I GWGCWC 2 cut(s) 198, 996
BbvI GCAGC 3 cut(s) 193, 557, 751
BccI CCATC 4 cut(s) 272, 440, 483, 1005
BciT130I CCWGG 2 cut(s) 106, 123
BclI TGATCA 1 cut(s) 256
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 743
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 3 cut(s) 440, 828, 954
BisI GCNGC 3 cut(s) 207, 546, 765
BlsI GCNGC 3 cut(s) 208, 547, 766
Bme1390I CCNGG 3 cut(s) 106, 123, 293
Bme18I GGWCC 3 cut(s) 90, 449, 1160
BmgT120I GGNCC 5 cut(s) 90, 449, 772, 937, 1160
BmrFI CCNGG 3 cut(s) 106, 123, 293
BmrI ACTGGG 1 cut(s) 569
BmsI GCATC 4 cut(s) 54, 532, 814, 1045
BmuI ACTGGG 1 cut(s) 569
BpiI GAAGAC 1 cut(s) 159
BplI GAGNNNNNCTC 4 cut(s) 103, 135, 983, 1015
BpmI CTGGAG 1 cut(s) 88
Bpu10I CCTNAGC 1 cut(s) 867
BpuEI CTTGAG 2 cut(s) 182, 646
BpuMI CCSGG 1 cut(s) 293
BsaJI CCNNGG 4 cut(s) 122, 161, 775, 898
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 716
Bse1I ACTGG 3 cut(s) 575, 710, 1016
BseBI CCWGG 2 cut(s) 106, 123
BseDI CCNNGG 4 cut(s) 122, 161, 775, 898
BseGI GGATG 3 cut(s) 451, 963, 1060
BseLI CCNNNNNNNGG 2 cut(s) 74, 716
BseMII CTCAG 4 cut(s) 101, 881, 1014, 1167
BseNI ACTGG 3 cut(s) 575, 710, 1016
BseRI GAGGAG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 368
BseXI GCAGC 3 cut(s) 193, 557, 751
BsgI GTGCAG 1 cut(s) 442
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 4 cut(s) 232, 773, 939, 1015
BsiHKAI GWGCWC 2 cut(s) 198, 996
BsiSI CCGG 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 74, 716
BsmAI GTCTC 1 cut(s) 743
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 4 cut(s) 232, 773, 939, 1015
Bsp1286I GDGCHC 3 cut(s) 198, 368, 996
Bsp143I GATC 2 cut(s) 256, 720
BspACI CCGC 3 cut(s) 388, 898, 900
BspANI GGCC 4 cut(s) 232, 773, 939, 1015
BspCNI CTCAG 4 cut(s) 102, 880, 1013, 1168
BspFNI CGCG 1 cut(s) 900
BspHI TCATGA 1 cut(s) 930
BspPI GGATC 1 cut(s) 728
BsrBI CCGCTC 1 cut(s) 388
BsrI ACTGG 3 cut(s) 575, 710, 1016
BssECI CCNNGG 4 cut(s) 122, 161, 775, 898
BssMI GATC 2 cut(s) 256, 720
BssT1I CCWWGG 2 cut(s) 161, 775
Bst2UI CCWGG 2 cut(s) 106, 123
Bst4CI ACNGT 3 cut(s) 562, 691, 754
Bst6I CTCTTC 1 cut(s) 168
BstC8I GCNNGC 4 cut(s) 204, 396, 864, 951
BstDEI CTNAG 5 cut(s) 56, 110, 867, 1000, 1176
BstDSI CCRYGG 1 cut(s) 898
BstF5I GGATG 3 cut(s) 451, 963, 1060
BstFNI CGCG 1 cut(s) 900
BstKTI GATC 2 cut(s) 259, 723
BstMAI GTCTC 1 cut(s) 743
BstMBI GATC 2 cut(s) 256, 720
BstMWI GCNNNNNNNGC 5 cut(s) 344, 404, 770, 1042, 1064
BstNI CCWGG 2 cut(s) 106, 123
BstSCI CCNGG 3 cut(s) 104, 121, 291
BstSLI GKGCMC 1 cut(s) 368
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 3 cut(s) 193, 557, 751
BstV2I GAAGAC 1 cut(s) 159
BsuRI GGCC 4 cut(s) 232, 773, 939, 1015
BtgI CCRYGG 1 cut(s) 898
BtsCI GGATG 3 cut(s) 451, 963, 1060
BtsI GCAGTG 1 cut(s) 1127
BtsIMutI CAGTG 2 cut(s) 1023, 1127
Cac8I GCNNGC 4 cut(s) 204, 396, 864, 951
CaiI CAGNNNCTG 3 cut(s) 146, 752, 1051
CciI TCATGA 1 cut(s) 930
Cfr13I GGNCC 5 cut(s) 90, 449, 772, 937, 1160
Cfr42I CCGCGG 1 cut(s) 901
Csp6I GTAC 1 cut(s) 571
CviAII CATG 2 cut(s) 601, 931
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 5 cut(s) 56, 110, 867, 1000, 1176
DpnI GATC 2 cut(s) 258, 722
DpnII GATC 2 cut(s) 256, 720
EaeI YGGCCR 1 cut(s) 1013
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 994
Eco130I CCWWGG 2 cut(s) 161, 775
Eco147I AGGCCT 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 996
Eco32I GATATC 1 cut(s) 892
Eco47I GGWCC 3 cut(s) 90, 449, 1160
Eco53kI GAGCTC 1 cut(s) 994
Eco57I CTGAAG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 994
EcoO109I RGGNCCY 1 cut(s) 1160
EcoRII CCWGG 2 cut(s) 104, 121
EcoRV GATATC 1 cut(s) 892
EcoT14I CCWWGG 2 cut(s) 161, 775
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 2 cut(s) 161, 775
FaeI CATG 2 cut(s) 604, 934
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FatI CATG 2 cut(s) 600, 930
FauI CCCGC 1 cut(s) 893
FauNDI CATATG 2 cut(s) 313, 529
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 3 cut(s) 207, 546, 765
FokI GGATG 3 cut(s) 458, 970, 1067
FriOI GRGCYC 1 cut(s) 996
Fsp4HI GCNGC 3 cut(s) 207, 546, 765
FspBI CTAG 3 cut(s) 440, 828, 954
GluI GCNGC 3 cut(s) 207, 546, 765
GsuI CTGGAG 1 cut(s) 88
HaeIII GGCC 4 cut(s) 232, 773, 939, 1015
HapII CCGG 1 cut(s) 292
Hin1II CATG 2 cut(s) 604, 934
HindIII AAGCTT 1 cut(s) 243
HinfI GANTC 3 cut(s) 731, 840, 1048
HpaII CCGG 1 cut(s) 292
HphI GGTGA 5 cut(s) 37, 146, 226, 338, 556
Hpy166II GTNNAC 2 cut(s) 28, 757
Hpy188I TCNGA 4 cut(s) 63, 172, 556, 883
Hpy188III TCNNGA 6 cut(s) 141, 482, 636, 809, 931, 1052
Hpy8I GTNNAC 2 cut(s) 28, 757
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 7 cut(s) 263, 420, 512, 601, 602, 928, 1173
HpyCH4III ACNGT 3 cut(s) 562, 691, 754
HpyCH4V TGCA 4 cut(s) 423, 545, 767, 989
HpyF10VI GCNNNNNNNGC 5 cut(s) 344, 404, 770, 1042, 1064
HpyF3I CTNAG 5 cut(s) 56, 110, 867, 1000, 1176
Hsp92II CATG 2 cut(s) 604, 934
Ksp22I TGATCA 1 cut(s) 256
KspI CCGCGG 1 cut(s) 901
Kzo9I GATC 2 cut(s) 256, 720
LmnI GCTCC 2 cut(s) 107, 143
Lsp1109I GCAGC 3 cut(s) 193, 557, 751
LweI GCATC 4 cut(s) 54, 532, 814, 1045
MaeI CTAG 3 cut(s) 440, 828, 954
MaeIII GTNAC 2 cut(s) 562, 907
MalI GATC 2 cut(s) 258, 722
MbiI CCGCTC 1 cut(s) 388
MboI GATC 2 cut(s) 256, 720
MboII GAAGA 7 cut(s) 5, 159, 185, 833, 839, 849, 886
MhlI GDGCHC 3 cut(s) 198, 368, 996
MlsI TGGCCA 1 cut(s) 1015
MluCI AATT 6 cut(s) 72, 408, 780, 1081, 1152, 1169
MluNI TGGCCA 1 cut(s) 1015
MmeI TCCRAC 2 cut(s) 673, 796
Mox20I TGGCCA 1 cut(s) 1015
MscI TGGCCA 1 cut(s) 1015
MseI TTAA 1 cut(s) 1172
Msp20I TGGCCA 1 cut(s) 1015
MspA1I CMGCKG 1 cut(s) 900
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 3 cut(s) 106, 123, 293
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 2 cut(s) 106, 123
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 5 cut(s) 344, 404, 770, 1042, 1064
NciI CCSGG 1 cut(s) 293
NdeI CATATG 2 cut(s) 313, 529
NdeII GATC 2 cut(s) 256, 720
NlaIII CATG 2 cut(s) 604, 934
NmeAIII GCCGAG 1 cut(s) 364
NmuCI GTSAC 2 cut(s) 562, 907
PagI TCATGA 1 cut(s) 930
PceI AGGCCT 1 cut(s) 232
PctI GAATGC 1 cut(s) 46
PfeI GAWTC 3 cut(s) 731, 840, 1048
PkrI GCNGC 3 cut(s) 208, 547, 766
PpuMI RGGWCCY 1 cut(s) 1160
Psp124BI GAGCTC 1 cut(s) 996
Psp5II RGGWCCY 1 cut(s) 1160
Psp6I CCWGG 2 cut(s) 104, 121
PspGI CCWGG 2 cut(s) 104, 121
PspPI GGNCC 5 cut(s) 90, 449, 772, 937, 1160
PspPPI RGGWCCY 1 cut(s) 1160
PstNI CAGNNNCTG 3 cut(s) 146, 752, 1051
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SacI GAGCTC 1 cut(s) 996
SacII CCGCGG 1 cut(s) 901
SaqAI TTAA 1 cut(s) 1172
SatI GCNGC 3 cut(s) 207, 546, 765
Sau3AI GATC 2 cut(s) 256, 720
Sau96I GGNCC 5 cut(s) 90, 449, 772, 937, 1160
ScrFI CCNGG 3 cut(s) 106, 123, 293
SduI GDGCHC 3 cut(s) 198, 368, 996
SfaNI GCATC 4 cut(s) 54, 532, 814, 1045
Sfr303I CCGCGG 1 cut(s) 901
SgrBI CCGCGG 1 cut(s) 901
SinI GGWCC 3 cut(s) 90, 449, 1160
SmlI CTYRAG 2 cut(s) 197, 625
SmoI CTYRAG 2 cut(s) 197, 625
SpeI ACTAGT 1 cut(s) 439
Sse9I AATT 6 cut(s) 72, 408, 780, 1081, 1152, 1169
SseBI AGGCCT 1 cut(s) 232
SsiI CCGC 3 cut(s) 388, 898, 900
SspMI CTAG 3 cut(s) 440, 828, 954
SstI GAGCTC 1 cut(s) 996
StuI AGGCCT 1 cut(s) 232
StyD4I CCNGG 3 cut(s) 104, 121, 291
StyI CCWWGG 2 cut(s) 161, 775
TaaI ACNGT 3 cut(s) 562, 691, 754
TaqI TCGA 2 cut(s) 743, 808
TasI AATT 6 cut(s) 72, 408, 780, 1081, 1152, 1169
TfiI GAWTC 3 cut(s) 731, 840, 1048
Tru1I TTAA 1 cut(s) 1172
Tru9I TTAA 1 cut(s) 1172
TscAI CASTG 2 cut(s) 1023, 1134
TseFI GTSAC 2 cut(s) 562, 907
TseI GCWGC 3 cut(s) 206, 545, 764
Tsp45I GTSAC 2 cut(s) 562, 907
TspDTI ATGAA 6 cut(s) 201, 450, 450, 669, 919, 947
TspRI CASTG 2 cut(s) 1023, 1134
VpaK11BI GGWCC 3 cut(s) 90, 449, 1160
XspI CTAG 3 cut(s) 440, 828, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.