RchiOBHm_Chr2g0152351

Phytoene synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003237
Physical Location & Seq
Forward (+)
69750842 .. 69753841
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52150

Sequence Viewer

Length: 1197 bp
ATGTCTGGTGTTCTTCTTTGGGTGGTGAGTCCCAAAGAGAATGCCACCTCTTTGCTTGGTCTTATGCCCAGAATTGGTAACCCAAAGAGGTCCAAGTTCTGCTCCAGGCTGAGTTTCTCACCGGGGATTTTAGCCTATTCAGGAGCTGTTGCTCACCCATCAAGGTCTTCAGAAGAGAGGGTCTATGAAGTGGTGCTCAAGCAAGCTGCTTTGGTGAGAGAGCAGAGGAGGCCCAAAGAGAAAGCTTTGGACTTTGGTGAAGGGGTTGAAAGTAGTGATGGGATGACCAACTGGGATTTGCTGAATGAGGCTTACGATAGGTGTGGTGAGGTTTGTGCTGAGTATGCCAAGACTTTTTACTTGGGCACATTACTTATGACACCAGAGCGGAGACGAGCGGTTTGGGCAATTTATGTATGGTGCAGAAGGACTGATGAACTAGTGGATGGACCTAATGCTTCATATATCACACCTAAAGCTCTTGACAGATGGGAAAAAAGACTGACTGACCTTTTTGATGGTCGTCCATATGATATGTATGATGCAGCTCTATCTGATACTGTCGCAAAGTACCCAGTGGATATACAGCCCTTCAAGGACATGGTAGAAGGAATGAGGTTAGACTTGAGAAAGTCAAGATACCAGAACTTTGATGAACTTTACCTCTACTGCTACTATGTTGCTGGAACTGTTGGACTGATGAGCGTTCCAGTAATGGGGATCTCCCCTGAATCAAAGGCTTCAACAGAGACTGTTTACAATGCTGCATTGGCCCTTGGAATTGCTAATCAGCTCACTAACATACTTAGAGATGTTGGAGAAGATGCTAGGAGAGGAAGAGTCTATCTCCCACAAGATGAGCTTGCCCAGGCTGGACTATCGGATGACGATATCTTTCGCGGGAAAGTGACAGACAAGTGGCAAAGTTTCATGAAAGACCAAATAAAGCGAGCTAGGATGTTCTTTGATGAGGCTGAAAAAGGTGTTGCAGAGCTCAACTCAGCAAGTAGATGGCCAGTATGGGCTTCTTTGTTGCTATATAGGCAGATTCTAGATGCCATTGAAGCAAATGGTTATGACAATTTCACAAAGAGGGCTTATGTAGGAAAAGCAAAAAAGTTGGTATCATTGCCAATAGCTTATGGCAGAGCAATTATAGGCCCCTCAAAATTAACTAAGCCGTTGGTGGCAAGATGA

Protein Analysis

398

Amino Acids

44.94

Weight (kDa)

8.73

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQS_PSY PF00494 114 - 370 5.1e-78 Squalene/phytoene synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 388, 398
AccII CGCG 1 cut(s) 900
AciI CCGC 3 cut(s) 388, 398, 900
AclWI GGATC 1 cut(s) 728
AcoI YGGCCR 1 cut(s) 1013
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 2 cut(s) 74, 716
AgsI TTSAA 4 cut(s) 269, 595, 744, 1064
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 2 cut(s) 104, 867
Alw21I GWGCWC 2 cut(s) 198, 996
Alw26I GTCTC 2 cut(s) 385, 743
AlwI GGATC 1 cut(s) 728
AlwNI CAGNNNCTG 2 cut(s) 146, 752
AoxI GGCC 4 cut(s) 230, 771, 1013, 1159
ApeKI GCWGC 3 cut(s) 206, 545, 764
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
AspS9I GGNCC 5 cut(s) 90, 231, 449, 772, 1160
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 6 cut(s) 37, 111, 146, 226, 269, 338
AvaII GGWCC 2 cut(s) 90, 449
BaeGI GKGCMC 1 cut(s) 368
BalI TGGCCA 1 cut(s) 1015
BanII GRGCYC 1 cut(s) 996
BbsI GAAGAC 1 cut(s) 159
Bbv12I GWGCWC 2 cut(s) 198, 996
BbvI GCAGC 3 cut(s) 193, 557, 751
BccI CCATC 6 cut(s) 166, 272, 440, 483, 512, 1005
BceAI ACGGC 1 cut(s) 1165
BciT130I CCWGG 2 cut(s) 106, 869
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 2 cut(s) 385, 743
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 4 cut(s) 440, 828, 954, 1052
BisI GCNGC 3 cut(s) 207, 546, 765
BlsI GCNGC 3 cut(s) 208, 547, 766
Bme1390I CCNGG 3 cut(s) 106, 123, 869
Bme18I GGWCC 2 cut(s) 90, 449
BmgT120I GGNCC 5 cut(s) 90, 231, 449, 772, 1160
BmiI GGNNCC 1 cut(s) 1162
BmrFI CCNGG 3 cut(s) 106, 123, 869
BmrI ACTGGG 2 cut(s) 301, 569
BmsI GCATC 3 cut(s) 532, 814, 1045
BmuI ACTGGG 2 cut(s) 301, 569
BpiI GAAGAC 1 cut(s) 159
BplI GAGNNNNNCTC 4 cut(s) 831, 863, 983, 1015
BpmI CTGGAG 1 cut(s) 88
BpuEI CTTGAG 2 cut(s) 182, 646
BpuMI CCSGG 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 122, 775, 867
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 716
Bse1I ACTGG 4 cut(s) 296, 575, 710, 1016
Bse3DI GCAATG 1 cut(s) 1127
BseBI CCWGG 2 cut(s) 106, 869
BseDI CCNNGG 3 cut(s) 122, 775, 867
BseGI GGATG 4 cut(s) 288, 451, 889, 963
BseLI CCNNNNNNNGG 2 cut(s) 74, 716
BseMI GCAATG 1 cut(s) 1127
BseMII CTCAG 3 cut(s) 101, 330, 1014
BseNI ACTGG 4 cut(s) 296, 575, 710, 1016
BseRI GAGGAG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 368
BseXI GCAGC 3 cut(s) 193, 557, 751
BsgI GTGCAG 1 cut(s) 442
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 4 cut(s) 232, 773, 1015, 1161
BsiHKAI GWGCWC 2 cut(s) 198, 996
BsiSI CCGG 1 cut(s) 122
BslFI GGGAC 1 cut(s) 15
BslI CCNNNNNNNGG 2 cut(s) 74, 716
BsmAI GTCTC 2 cut(s) 385, 743
BsmBI CGTCTC 1 cut(s) 385
BsmFI GGGAC 1 cut(s) 15
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 4 cut(s) 232, 773, 1015, 1161
Bsp1286I GDGCHC 3 cut(s) 198, 368, 996
Bsp143I GATC 1 cut(s) 720
BspACI CCGC 3 cut(s) 388, 398, 900
BspANI GGCC 4 cut(s) 232, 773, 1015, 1161
BspCNI CTCAG 3 cut(s) 102, 331, 1013
BspFNI CGCG 1 cut(s) 900
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 1 cut(s) 1162
BspPI GGATC 1 cut(s) 728
BsrBI CCGCTC 2 cut(s) 388, 398
BsrDI GCAATG 1 cut(s) 1127
BsrI ACTGG 4 cut(s) 296, 575, 710, 1016
BssECI CCNNGG 3 cut(s) 122, 775, 867
BssMI GATC 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 775
Bst2UI CCWGG 2 cut(s) 106, 869
Bst4CI ACNGT 3 cut(s) 562, 691, 754
Bst6I CTCTTC 2 cut(s) 168, 832
BstC8I GCNNGC 3 cut(s) 204, 864, 951
BstDEI CTNAG 5 cut(s) 110, 339, 806, 1000, 1176
BstEII GGTNACC 1 cut(s) 77
BstF5I GGATG 4 cut(s) 288, 451, 889, 963
BstFNI CGCG 1 cut(s) 900
BstKTI GATC 1 cut(s) 723
BstMAI GTCTC 2 cut(s) 385, 743
BstMBI GATC 1 cut(s) 720
BstMWI GCNNNNNNNGC 6 cut(s) 229, 344, 404, 770, 1042, 1064
BstNI CCWGG 2 cut(s) 106, 869
BstPI GGTNACC 1 cut(s) 77
BstSCI CCNGG 3 cut(s) 104, 121, 867
BstSLI GKGCMC 1 cut(s) 368
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 3 cut(s) 193, 557, 751
BstV2I GAAGAC 1 cut(s) 159
BstX2I RGATCY 1 cut(s) 720
BstYI RGATCY 1 cut(s) 720
BsuRI GGCC 4 cut(s) 232, 773, 1015, 1161
BtsCI GGATG 4 cut(s) 288, 451, 889, 963
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 3 cut(s) 204, 864, 951
CaiI CAGNNNCTG 2 cut(s) 146, 752
CciI TCATGA 1 cut(s) 930
Cfr13I GGNCC 5 cut(s) 90, 231, 449, 772, 1160
Csp6I GTAC 1 cut(s) 571
CviAII CATG 2 cut(s) 601, 931
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 5 cut(s) 110, 339, 806, 1000, 1176
DpnI GATC 1 cut(s) 722
DpnII GATC 1 cut(s) 720
EaeI YGGCCR 1 cut(s) 1013
Eam1104I CTCTTC 2 cut(s) 168, 832
EarI CTCTTC 2 cut(s) 168, 832
Ecl136II GAGCTC 1 cut(s) 994
Eco130I CCWWGG 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 996
Eco32I GATATC 1 cut(s) 892
Eco47I GGWCC 2 cut(s) 90, 449
Eco53kI GAGCTC 1 cut(s) 994
Eco57I CTGAAG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 77
EcoICRI GAGCTC 1 cut(s) 994
EcoO109I RGGNCCY 1 cut(s) 1160
EcoO65I GGTNACC 1 cut(s) 77
EcoRII CCWGG 2 cut(s) 104, 867
EcoRV GATATC 1 cut(s) 892
EcoT14I CCWWGG 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 1 cut(s) 775
Esp3I CGTCTC 1 cut(s) 385
FaeI CATG 2 cut(s) 604, 934
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FaqI GGGAC 1 cut(s) 15
FatI CATG 2 cut(s) 600, 930
FauI CCCGC 1 cut(s) 893
FauNDI CATATG 1 cut(s) 529
Fnu4HI GCNGC 3 cut(s) 207, 546, 765
FokI GGATG 4 cut(s) 295, 458, 896, 970
FriOI GRGCYC 1 cut(s) 996
Fsp4HI GCNGC 3 cut(s) 207, 546, 765
FspBI CTAG 4 cut(s) 440, 828, 954, 1052
GluI GCNGC 3 cut(s) 207, 546, 765
GsuI CTGGAG 1 cut(s) 88
HaeIII GGCC 4 cut(s) 232, 773, 1015, 1161
HapII CCGG 1 cut(s) 122
Hin1II CATG 2 cut(s) 604, 934
HindIII AAGCTT 1 cut(s) 243
HinfI GANTC 4 cut(s) 28, 731, 840, 1048
HpaII CCGG 1 cut(s) 122
HphI GGTGA 6 cut(s) 37, 111, 146, 226, 269, 338
Hpy166II GTNNAC 1 cut(s) 757
Hpy188I TCNGA 3 cut(s) 172, 556, 883
Hpy188III TCNNGA 5 cut(s) 141, 482, 636, 931, 1052
Hpy8I GTNNAC 1 cut(s) 757
HpyAV CCTTC 4 cut(s) 254, 420, 601, 602
HpyCH4III ACNGT 3 cut(s) 562, 691, 754
HpyCH4V TGCA 4 cut(s) 423, 545, 767, 989
HpyF10VI GCNNNNNNNGC 6 cut(s) 229, 344, 404, 770, 1042, 1064
HpyF3I CTNAG 5 cut(s) 110, 339, 806, 1000, 1176
Hsp92II CATG 2 cut(s) 604, 934
Kzo9I GATC 1 cut(s) 720
LmnI GCTCC 2 cut(s) 107, 143
Lsp1109I GCAGC 3 cut(s) 193, 557, 751
LweI GCATC 3 cut(s) 532, 814, 1045
MaeI CTAG 4 cut(s) 440, 828, 954, 1052
MaeIII GTNAC 2 cut(s) 77, 907
MalI GATC 1 cut(s) 722
MbiI CCGCTC 2 cut(s) 388, 398
MboI GATC 1 cut(s) 720
MboII GAAGA 5 cut(s) 5, 159, 185, 833, 849
MflI RGATCY 1 cut(s) 720
MhlI GDGCHC 3 cut(s) 198, 368, 996
MlsI TGGCCA 1 cut(s) 1015
MluCI AATT 6 cut(s) 72, 408, 780, 1081, 1152, 1169
MluNI TGGCCA 1 cut(s) 1015
MlyI GAGTC 2 cut(s) 37, 849
MmeI TCCRAC 2 cut(s) 673, 796
Mox20I TGGCCA 1 cut(s) 1015
MscI TGGCCA 1 cut(s) 1015
MseI TTAA 1 cut(s) 1172
Msp20I TGGCCA 1 cut(s) 1015
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 3 cut(s) 106, 123, 869
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 2 cut(s) 106, 869
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 6 cut(s) 229, 344, 404, 770, 1042, 1064
NciI CCSGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 529
NdeII GATC 1 cut(s) 720
NlaIII CATG 2 cut(s) 604, 934
NlaIV GGNNCC 1 cut(s) 1162
NmuCI GTSAC 1 cut(s) 907
PagI TCATGA 1 cut(s) 930
PctI GAATGC 1 cut(s) 46
PfeI GAWTC 2 cut(s) 731, 1048
PkrI GCNGC 3 cut(s) 208, 547, 766
PleI GAGTC 2 cut(s) 36, 848
PpsI GAGTC 2 cut(s) 36, 848
Psp124BI GAGCTC 1 cut(s) 996
Psp6I CCWGG 2 cut(s) 104, 867
PspEI GGTNACC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 104, 867
PspN4I GGNNCC 1 cut(s) 1162
PspPI GGNCC 5 cut(s) 90, 231, 449, 772, 1160
PstNI CAGNNNCTG 2 cut(s) 146, 752
PsuI RGATCY 1 cut(s) 720
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SacI GAGCTC 1 cut(s) 996
SaqAI TTAA 1 cut(s) 1172
SatI GCNGC 3 cut(s) 207, 546, 765
Sau3AI GATC 1 cut(s) 720
Sau96I GGNCC 5 cut(s) 90, 231, 449, 772, 1160
SchI GAGTC 2 cut(s) 37, 849
ScrFI CCNGG 3 cut(s) 106, 123, 869
SduI GDGCHC 3 cut(s) 198, 368, 996
SfaNI GCATC 3 cut(s) 532, 814, 1045
SinI GGWCC 2 cut(s) 90, 449
SmlI CTYRAG 2 cut(s) 197, 625
SmoI CTYRAG 2 cut(s) 197, 625
SpeI ACTAGT 1 cut(s) 439
Sse9I AATT 6 cut(s) 72, 408, 780, 1081, 1152, 1169
SsiI CCGC 3 cut(s) 388, 398, 900
SspMI CTAG 4 cut(s) 440, 828, 954, 1052
SstI GAGCTC 1 cut(s) 996
StyD4I CCNGG 3 cut(s) 104, 121, 867
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 3 cut(s) 562, 691, 754
TasI AATT 6 cut(s) 72, 408, 780, 1081, 1152, 1169
TfiI GAWTC 2 cut(s) 731, 1048
Tru1I TTAA 1 cut(s) 1172
Tru9I TTAA 1 cut(s) 1172
TscAI CASTG 1 cut(s) 582
TseFI GTSAC 1 cut(s) 907
TseI GCWGC 3 cut(s) 206, 545, 764
Tsp45I GTSAC 1 cut(s) 907
TspDTI ATGAA 6 cut(s) 201, 450, 450, 669, 919, 947
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 2 cut(s) 90, 449
XbaI TCTAGA 1 cut(s) 1051
XspI CTAG 4 cut(s) 440, 828, 954, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.