Rorug03G0238700

Phytoene synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003237
Physical Location & Seq
Reverse (-)
22231778 .. 22243079
11302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0238700.1

Sequence Viewer

Length: 3252 bp
ATGGAACCAGGAGGAGAGAGGACAATGTTTAAGAATAAAGCTAGTGAGCAAGATAAGCAAAGTGTTTCTTTTTACAAGTTGTTTATGTTTGCTGATCGGTATGACATTTCATTAATGGTTGTCGGAAGTCTGAGTGCGATTGGAAATGGGATATCGCAGCCCCTTATGACGCTCGTCTTTGGTGGTCTTATCAATACTTTTGGAGCTACTGACCCAGCTCATATCGTCCCTATAGTTTCTCAAGTGTCTCTAAAATTTTTATATTTGGCCATTGGTACCGGTCTTGCTGCATTTTTGCAGGTGTCTTGTTGGATGGTGACTGGGGAAAGACAAGCTACTCGAATTAGAGGCTTGTACTTGAAAACAATACTAAGACAAGATATTGGATTTTTTGACACCCAAACTAACACTGGAGAGATCATTGGGAGAATGTCAGGGGACACCATTCTCATTCAAGATGCCATGAGAGAAAAGGTGGGGAAGTTTATACAACTCATTTCAACTTTTTTCGGCGGTTTTGTAATTGCTTTTATCAAAGGATGGCTTCTAAACTTGGTATTGCTATCCTGCATTCCTGTTATTGTCATTGCTGGTGGAGTCATGGCCACTGTTGTTTCAAAAATGTCAACATGCGGGCAACGTGCTTACGCGGAAGCTGGTAGTATAGTAGAACAGACTGTCGGAGCCATCAGAACAGTTGCATCTTTTACTGGAGAGAGGCAAGCGATAGAAAAATACAACCAAAAGCTGAAAATTGCCTACAAAAACATGGTTCAACAAGGACTAGCCACAGGGATTGGACTTGGTACATTCATGCTTATTGTATTTAGCACTTATGCACTTGCCATATGGTACGGATCCAAAATGATCATTGAGAAAGGCTATAACGGGGGCCAAGTCATTAATGTTCTATTTGCAATGATGGCTGGTGGAATGTCACTAGGACGGACATCACCGTCTCTCAATGCATTTACAGCAGGGAAGGCTGCTGCATACAAAATGCTTGAGACCATCAAGCGAATACCAACGATTGATCCCTATGATACTACTGGAGTTGTTATTAAAGGAGAGGTCGAACTTAAAGATGTGTACTTCAGGTATCCTGCTAGGCCAGATGTGCAGATCTTTGCTGGATTCTCATTACATGTTCCAAATGGCACGACGACTGCTTTGGTTGGGCAGAGTGGAAGTGGGAAATCAACAGTGATAAGCCTGGTAGAAAGATTCTACGATCCAGAAGCTGGTGAAGTGCTTATAGATGGTGTCGATTTGAAGAATTTACATCTTAGATCAATAAGGGAAAGGATTGGGCTTGAGATTAGAAGAGCCACAGAGCTTGCAAATGCAGCAAAGTTCATTGATAAGCTGCCCCAGGGGCTTGACACAATGGTAGGTGAGCATGGAACATCATTATCGGGTGGACAAAAGCAAATAATTGCAATTGCTAGAGCCATTTTGAAAAACCCAAGAATTCTGCTTCTCGACGAAGCTACAAGTGCTCTAGATACTGTGTCTGAACGAATAGTTCAAGATGCACTTGTGAGATTAATGTCAAATAGGACAATTATTGTTGTTGCTCATCGCTTGACAACGATTAGAAATGCTGATGCAATAGCAGTGGAGAGCCTTGGAAAACCTAAGGTTGATCCTGAAGAGCGTAAAACAGTTTCCATCAAAAGGTTGGCAATGCTGAACAAAAATGAGCTTCCAATTTTGCTTCTTGGCGCCATTGCTGCAGCTGGTCATGGAGTAATTTTTCCTGTTTTCGGCTTACTACTCTCAAAAGCCATTAAAATGTTCTATGAACCACATAACGAGCTACGAGAAGACTCAAAAACATGGGCAGGAGTGTATGTAGGGATGGGATGCTTTGCACTGGCTGTCATTCCAGTGCAGAATATCTTATTTGCAATTGCAGGTGGAAAATTGATAAAGCAAATTTGTTCCTTGACATTCCAGAAGGTTGTGCACCAACAAATCAGTTGGTTTGATGATCCTGCAAATTCAAGCGGTGCCATTGGTGCAAGGTTGTCTTCTGATGCTTCAACCGTGAAGGCTCTTGTTGGTGATGCTTTAGCATTGATTGCACAGAATATAGCAACAGTCATAGCAGGGTTGATTATAGGTTTTACAGCTAACTGGAAACTAGCGCTTATAATTTTAGCCATGTCGCCATTGCTGATTATGCGAGTACTGCAAACAAAATTTCTTCAAGGGTTTAGTGCAGATGCTAAGGTGATGGAAGCCTACGAGAAGAAGTGCGATGCTCCTATGAAACAAGGAGTACGGTTAGGAGTAGTGAGTGGAGTAGGTTTTGGCTTCTCGTTCTTCGTAATGTTCTGTACAAATGCGCTAATTTTCTATATTGGAGCTATTCTTGTGAAAAATGGGCAAAGCTACATATTCTTTGCTTTGACAATCTCAGCAGTAGGGGTTTCAGAATCTAGTGGTATGGGTCCTGACTCCAACAAAGCAAAGGACTCAGCAACTTCGATATTTAAGATTCTTGACAGCAAGCCTATGATTGATTCGAGCAGCGATACAGGCATAACATTATCGAGCATAACCGGAGAAATTGATCTCAAGCATGTTAGCTTCAAATATCCCACAAGGCCAGATGTGCAGATATTTAAAGACATTTGTTTGAAAATACCCTCTGGAAAGACTGTTGCTTTGGTTGGAGAGAGTGGTCGTGTACTATTAGATGGAGTGGAAATCAACAAGTTCAAGCTAAACTGGTTGAGGCAACAAATAGGTTTGGTTGGTCAAGAACCAATTCTCTTTAACGAATCCATTCGGACTAACATTGCTTATGGCAAGCGTGGAGATGTAACTGAAGAAGAGATCATTGCTGCCACAAAAGCATCAAATGCACACACCTTCATATATTTACTACCTCAAGGCTATGACACCTTTGTTGGGGAACGAGGGGTTCAACTATCTGGAGGGCAAAAGCAAAGAATAGCCATTGCAAGGGCTATTCTAAAAGACCCGAAAATCCTCTTGCTCGATGAAGCTACAAGTGCACTAGATGCAGAATCAGAACGCATAGTACAAGATGCTTTGGACAGTGTTATGGTGAATAGAACAACTGTGGTCGTTGCTCACCGCCTTACCACGATTAAAGGGGCAGACATAATTGCTGTGGTGAAGAACGACGTCATTGCTGAGAAGGGTAGCCATGATTCTCTAATGAATATCACTGAAGGTGCTTATGCATCTTTGGTGGCACTTCATTCTAACTCAACATGA

Protein Analysis

1083

Amino Acids

117.04

Weight (kDa)

9.08

Isoelectric Point (pI)

32.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 42 - 309 1.1e-57 ABC transporter transmembrane region
ABC_tran PF00005 378 - 499 2.8e-24 ABC transporter
ABC_ATPase PF09818 381 - 533 3.3e-06 P-loop domain
SMC_N PF02463 414 - 538 4.1e-06 RecF/RecN/SMC N terminal domain
ABC_membrane PF00664 572 - 729 6.5e-30 ABC transporter transmembrane region
ABC_membrane PF00664 737 - 798 3e-06 ABC transporter transmembrane region
ABC_tran PF00005 895 - 1008 3.3e-17 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2156
AarI CACCTGC 2 cut(s) 289, 1907
AasI GACNNNNNNGTC 1 cut(s) 955
AatII GACGTC 1 cut(s) 3162
Acc36I ACCTGC 2 cut(s) 289, 1907
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 3 cut(s) 275, 1724, 2012
AccB7I CCANNNNNTGG 1 cut(s) 1241
AccII CGCG 1 cut(s) 650
AciI CCGC 5 cut(s) 513, 633, 650, 2010, 3109
AclWI GGATC 6 cut(s) 852, 865, 1028, 1226, 1640, 1988
AcoI YGGCCR 2 cut(s) 267, 603
AcsI RAATTY 6 cut(s) 254, 1276, 1470, 1938, 2002, 2204
AcuI CTGAAG 4 cut(s) 1078, 1671, 2856, 3225
AcyI GRCGYC 2 cut(s) 1725, 3159
AfeI AGCGCT 1 cut(s) 2151
AfiI CCNNNNNNNGG 5 cut(s) 1241, 1677, 1766, 2919, 2973
AflIII ACRYGT 1 cut(s) 1144
AgeI ACCGGT 1 cut(s) 278
AjnI CCWGG 3 cut(s) 7, 1212, 1371
Alw21I GWGCWC 3 cut(s) 1501, 1971, 3028
Alw26I GTCTC 3 cut(s) 252, 963, 1001
Alw44I GTGCAC 2 cut(s) 1967, 3024
AlwI GGATC 6 cut(s) 852, 865, 1028, 1226, 1640, 1988
AlwNI CAGNNNCTG 1 cut(s) 1241
Aor51HI AGCGCT 1 cut(s) 2151
AoxI GGCC 5 cut(s) 267, 603, 892, 1109, 2612
ApaLI GTGCAC 2 cut(s) 1967, 3024
ApoI RAATTY 6 cut(s) 254, 1276, 1470, 1938, 2002, 2204
ArsI GACNNNNNNTTYG 4 cut(s) 1153, 1185, 2656, 2688
AseI ATTAAT 3 cut(s) 113, 903, 1547
AsiGI ACCGGT 1 cut(s) 278
Asp700I GAANNNNTTC 2 cut(s) 2775, 2793
Asp718I GGTACC 1 cut(s) 275
AspLEI GCGC 3 cut(s) 1727, 2152, 2353
AspS9I GGNCC 2 cut(s) 892, 2456
AsuHPI GGTGA 9 cut(s) 328, 945, 1256, 1406, 2078, 2248, 3091, 3098, 3160
AvaII GGWCC 1 cut(s) 2456
AxyI CCTNAGG 1 cut(s) 1638
BaeGI GKGCMC 2 cut(s) 1971, 3028
BalI TGGCCA 2 cut(s) 269, 605
BamHI GGATCC 1 cut(s) 857
BanI GGYRCC 3 cut(s) 275, 1724, 2012
BbsI GAAGAC 2 cut(s) 1833, 2025
Bbv12I GWGCWC 3 cut(s) 1501, 1971, 3028
BcgI CGANNNNNNTGC 2 cut(s) 3146, 3180
BciT130I CCWGG 3 cut(s) 9, 1214, 1373
BciVI GTATCC 1 cut(s) 1110
BclI TGATCA 1 cut(s) 867
BcoDI GTCTC 3 cut(s) 252, 963, 1001
BfaI CTAG 9 cut(s) 42, 785, 941, 1107, 1446, 1502, 2147, 2445, 3029
BfmI CTRYAG 2 cut(s) 231, 1734
BfoI RGCGCY 2 cut(s) 1728, 2153
BfuAI ACCTGC 2 cut(s) 289, 1907
BfuI GTATCC 1 cut(s) 1110
BglI GCCNNNNNGGC 1 cut(s) 1375
BglII AGATCT 1 cut(s) 1122
BmcAI AGTACT 1 cut(s) 2193
Bme1390I CCNGG 3 cut(s) 9, 1214, 1373
Bme18I GGWCC 1 cut(s) 2456
BmgT120I GGNCC 2 cut(s) 892, 2456
BmiI GGNNCC 8 cut(s) 6, 277, 685, 859, 893, 1726, 2014, 2457
BmrFI CCNGG 3 cut(s) 9, 1214, 1373
BmrI ACTGGG 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 330
BoxI GACNNNNGTC 1 cut(s) 173
BpiI GAAGAC 2 cut(s) 1833, 2025
BpmI CTGGAG 4 cut(s) 432, 732, 1071, 2964
Bpu10I CCTNAGC 1 cut(s) 2232
BpuEI CTTGAG 5 cut(s) 225, 1025, 1334, 2567, 2883
BsaHI GRCGYC 2 cut(s) 1725, 3159
BsaI GGTCTC 1 cut(s) 1001
BsaJI CCNNGG 3 cut(s) 1371, 1372, 1627
BsaWI WCCGGW 2 cut(s) 278, 2567
BsaXI ACNNNNNCTCC 2 cut(s) 1839, 1869
Bsc4I CCNNNNNNNGG 5 cut(s) 1241, 1677, 1766, 2919, 2973
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 8 cut(s) 325, 415, 715, 1054, 1881, 1889, 2144, 2741
Bse21I CCTNAGG 1 cut(s) 1638
Bse3DI GCAATG 9 cut(s) 585, 924, 1692, 1728, 2174, 2805, 2847, 2967, 3162
BseBI CCWGG 3 cut(s) 9, 1214, 1373
BseDI CCNNGG 3 cut(s) 1371, 1372, 1627
BseGI GGATG 4 cut(s) 318, 545, 1866, 1871
BseLI CCNNNNNNNGG 5 cut(s) 1241, 1677, 1766, 2919, 2973
BseMI GCAATG 9 cut(s) 585, 924, 1692, 1728, 2174, 2805, 2847, 2967, 3162
BseMII CTCAG 4 cut(s) 122, 2436, 2496, 3159
BseNI ACTGG 8 cut(s) 325, 415, 715, 1054, 1881, 1889, 2144, 2741
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 2 cut(s) 1971, 3028
BseYI CCCAGC 1 cut(s) 214
BsgI GTGCAG 4 cut(s) 1139, 1913, 2244, 2642
Bsh1236I CGCG 1 cut(s) 650
BshFI GGCC 5 cut(s) 269, 605, 894, 1111, 2614
BshNI GGYRCC 3 cut(s) 275, 1724, 2012
BshTI ACCGGT 1 cut(s) 278
BsiHKAI GWGCWC 3 cut(s) 1501, 1971, 3028
BsiSI CCGG 2 cut(s) 279, 2568
BslFI GGGAC 2 cut(s) 212, 452
BslI CCNNNNNNNGG 5 cut(s) 1241, 1677, 1766, 2919, 2973
BsmAI GTCTC 3 cut(s) 252, 963, 1001
BsmBI CGTCTC 1 cut(s) 963
BsmFI GGGAC 2 cut(s) 212, 452
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 5 cut(s) 269, 605, 894, 1111, 2614
Bso31I GGTCTC 1 cut(s) 1001
Bsp1286I GDGCHC 3 cut(s) 1501, 1971, 3028
Bsp1407I TGTACA 1 cut(s) 2342
BspACI CCGC 5 cut(s) 513, 633, 650, 2010, 3109
BspANI GGCC 5 cut(s) 269, 605, 894, 1111, 2614
BspCNI CTCAG 4 cut(s) 123, 2435, 2495, 3160
BspFNI CGCG 1 cut(s) 650
BspLI GGNNCC 8 cut(s) 6, 277, 685, 859, 893, 1726, 2014, 2457
BspMAI CTGCAG 1 cut(s) 1738
BspMI ACCTGC 2 cut(s) 289, 1907
BspPI GGATC 6 cut(s) 852, 865, 1028, 1226, 1640, 1988
BspQI GCTCTTC 2 cut(s) 1318, 1647
BspT107I GGYRCC 3 cut(s) 275, 1724, 2012
BspTNI GGTCTC 1 cut(s) 1001
BsrDI GCAATG 9 cut(s) 585, 924, 1692, 1728, 2174, 2805, 2847, 2967, 3162
BsrFI RCCGGY 1 cut(s) 278
BsrGI TGTACA 1 cut(s) 2342
BsrI ACTGG 8 cut(s) 325, 415, 715, 1054, 1881, 1889, 2144, 2741
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 3 cut(s) 1371, 1372, 1627
BssNI GRCGYC 2 cut(s) 1725, 3159
BssT1I CCWWGG 1 cut(s) 1627
Bst2UI CCWGG 3 cut(s) 9, 1214, 1373
Bst6I CTCTTC 3 cut(s) 1318, 1647, 2835
BstACI GRCGYC 2 cut(s) 1725, 3159
BstAPI GCANNNNNTGC 3 cut(s) 2084, 2870, 3032
BstAUI TGTACA 1 cut(s) 2342
BstC8I GCNNGC 5 cut(s) 635, 723, 1338, 2516, 2819
BstDEI CTNAG 8 cut(s) 131, 371, 1286, 1638, 2232, 2422, 2482, 3168
BstF5I GGATG 4 cut(s) 318, 545, 1866, 1871
BstFNI CGCG 1 cut(s) 650
BstH2I RGCGCY 2 cut(s) 1728, 2153
BstHHI GCGC 3 cut(s) 1727, 2152, 2353
BstMAI GTCTC 3 cut(s) 252, 963, 1001
BstNI CCWGG 3 cut(s) 9, 1214, 1373
BstNSI RCATGY 3 cut(s) 633, 1148, 2591
BstPAI GACNNNNGTC 1 cut(s) 173
BstSCI CCNGG 3 cut(s) 7, 1212, 1371
BstSFI CTRYAG 2 cut(s) 231, 1734
BstSLI GKGCMC 2 cut(s) 1971, 3028
BstUI CGCG 1 cut(s) 650
BstV2I GAAGAC 2 cut(s) 1833, 2025
BstX2I RGATCY 2 cut(s) 857, 1122
BstYI RGATCY 2 cut(s) 857, 1122
Bsu36I CCTNAGG 1 cut(s) 1638
BsuI GTATCC 1 cut(s) 1110
BsuRI GGCC 5 cut(s) 269, 605, 894, 1111, 2614
BtgZI GCGATG 2 cut(s) 1565, 2277
BtsCI GGATG 4 cut(s) 318, 545, 1866, 1871
BtsI GCAGTG 1 cut(s) 1623
BtsIMutI CAGTG 8 cut(s) 408, 606, 1209, 1623, 1874, 1896, 3076, 3201
BveI ACCTGC 2 cut(s) 289, 1907
Cac8I GCNNGC 5 cut(s) 635, 723, 1338, 2516, 2819
CaiI CAGNNNCTG 1 cut(s) 1241
CfoI GCGC 3 cut(s) 1727, 2152, 2353
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 2 cut(s) 892, 2456
CseI GACGC 1 cut(s) 178
CspAI ACCGGT 1 cut(s) 278
CspCI CAANNNNNGTGG 6 cut(s) 778, 813, 1318, 1353, 1599, 1634
DdeI CTNAG 8 cut(s) 131, 371, 1286, 1638, 2232, 2422, 2482, 3168
DinI GGCGCC 1 cut(s) 1726
DraI TTTAAA 1 cut(s) 2632
DrdI GACNNNNNNGTC 1 cut(s) 955
DseDI GACNNNNNNGTC 1 cut(s) 955
EaeI YGGCCR 2 cut(s) 267, 603
Eam1104I CTCTTC 3 cut(s) 1318, 1647, 2835
EarI CTCTTC 3 cut(s) 1318, 1647, 2835
Eco130I CCWWGG 1 cut(s) 1627
Eco31I GGTCTC 1 cut(s) 1001
Eco32I GATATC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 2456
Eco47III AGCGCT 1 cut(s) 2151
Eco57I CTGAAG 4 cut(s) 1078, 1671, 2856, 3225
Eco81I CCTNAGG 1 cut(s) 1638
EcoO109I RGGNCCY 1 cut(s) 2456
EcoRI GAATTC 1 cut(s) 1470
EcoRII CCWGG 3 cut(s) 7, 1212, 1371
EcoRV GATATC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 1627
EcoT22I ATGCAT 2 cut(s) 970, 3220
EgeI GGCGCC 1 cut(s) 1726
EheI GGCGCC 1 cut(s) 1726
ErhI CCWWGG 1 cut(s) 1627
Esp3I CGTCTC 1 cut(s) 963
FalI AAGNNNNNCTT 8 cut(s) 52, 84, 528, 560, 1521, 1553, 2017, 2049
FaqI GGGAC 2 cut(s) 212, 452
FauI CCCGC 1 cut(s) 626
FauNDI CATATG 1 cut(s) 848
FbaI TGATCA 1 cut(s) 867
FokI GGATG 4 cut(s) 325, 552, 1873, 1878
FspBI CTAG 9 cut(s) 42, 785, 941, 1107, 1446, 1502, 2147, 2445, 3029
GlaI GCGC 3 cut(s) 1726, 2151, 2352
GsaI CCCAGC 1 cut(s) 218
GsuI CTGGAG 4 cut(s) 432, 732, 1071, 2964
HaeII RGCGCY 2 cut(s) 1728, 2153
HaeIII GGCC 5 cut(s) 269, 605, 894, 1111, 2614
HapII CCGG 2 cut(s) 279, 2568
HgaI GACGC 1 cut(s) 178
HhaI GCGC 3 cut(s) 1727, 2152, 2353
Hin1I GRCGYC 2 cut(s) 1725, 3159
Hin6I GCGC 3 cut(s) 1725, 2150, 2351
HinP1I GCGC 3 cut(s) 1725, 2150, 2351
HincII GTYRAC 1 cut(s) 627
HindII GTYRAC 1 cut(s) 627
HpaII CCGG 2 cut(s) 279, 2568
HphI GGTGA 9 cut(s) 328, 945, 1256, 1406, 2078, 2248, 3091, 3098, 3160
Hpy166II GTNNAC 6 cut(s) 627, 1090, 1421, 1969, 2696, 3026
Hpy188I TCNGA 9 cut(s) 125, 132, 683, 692, 1516, 2038, 2440, 2799, 3043
Hpy8I GTNNAC 6 cut(s) 627, 1090, 1421, 1969, 2696, 3026
Hpy99I CGWCG 3 cut(s) 1165, 1487, 3161
HpyAV CCTTC 6 cut(s) 976, 1954, 2047, 2890, 3166, 3200
HpyCH4IV ACGT 2 cut(s) 640, 3159
HpyF3I CTNAG 8 cut(s) 131, 371, 1286, 1638, 2232, 2422, 2482, 3168
HpySE526I ACGT 2 cut(s) 640, 3159
Hsp92I GRCGYC 2 cut(s) 1725, 3159
HspAI GCGC 3 cut(s) 1725, 2150, 2351
KasI GGCGCC 1 cut(s) 1724
KpnI GGTACC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 867
LguI GCTCTTC 2 cut(s) 1318, 1647
LmnI GCTCC 4 cut(s) 203, 683, 2272, 2369
MaeI CTAG 9 cut(s) 42, 785, 941, 1107, 1446, 1502, 2147, 2445, 3029
MaeII ACGT 2 cut(s) 640, 3159
MaeIII GTNAC 3 cut(s) 316, 936, 2830
MfeI CAATTG 2 cut(s) 1440, 1911
MflI RGATCY 2 cut(s) 857, 1122
MhlI GDGCHC 3 cut(s) 1501, 1971, 3028
MlsI TGGCCA 2 cut(s) 269, 605
MluNI TGGCCA 2 cut(s) 269, 605
Mly113I GGCGCC 1 cut(s) 1725
MlyI GAGTC 4 cut(s) 606, 1823, 2456, 2474
MmeI TCCRAC 5 cut(s) 103, 290, 661, 2490, 2659
Mox20I TGGCCA 2 cut(s) 269, 605
Mph1103I ATGCAT 2 cut(s) 970, 3220
MroXI GAANNNNTTC 2 cut(s) 2775, 2793
MscI TGGCCA 2 cut(s) 269, 605
MslI CAYNNNNRTG 2 cut(s) 1793, 1889
Msp20I TGGCCA 2 cut(s) 269, 605
MspA1I CMGCKG 1 cut(s) 1739
MspI CCGG 2 cut(s) 279, 2568
MspR9I CCNGG 3 cut(s) 9, 1214, 1373
MunI CAATTG 2 cut(s) 1440, 1911
Mva1269I GAATGC 1 cut(s) 570
MvaI CCWGG 3 cut(s) 9, 1214, 1373
MvnI CGCG 1 cut(s) 650
NarI GGCGCC 1 cut(s) 1725
NdeI CATATG 1 cut(s) 848
NlaIV GGNNCC 8 cut(s) 6, 277, 685, 859, 893, 1726, 2014, 2457
NmuCI GTSAC 2 cut(s) 316, 936
NsiI ATGCAT 2 cut(s) 970, 3220
NspI RCATGY 3 cut(s) 633, 1148, 2591
PaqCI CACCTGC 2 cut(s) 289, 1907
PasI CCCWGGG 1 cut(s) 1372
PciI ACATGT 1 cut(s) 1144
PciSI GCTCTTC 2 cut(s) 1318, 1647
PctI GAATGC 1 cut(s) 570
PdmI GAANNNNTTC 2 cut(s) 2775, 2793
PfeI GAWTC 8 cut(s) 1134, 1224, 2441, 2503, 2528, 2789, 3038, 3185
PflMI CCANNNNNTGG 1 cut(s) 1241
PinAI ACCGGT 1 cut(s) 278
PleI GAGTC 4 cut(s) 605, 1823, 2456, 2474
PluTI GGCGCC 1 cut(s) 1728
PpsI GAGTC 4 cut(s) 605, 1823, 2456, 2474
PpuMI RGGWCCY 1 cut(s) 2456
PscI ACATGT 1 cut(s) 1144
PshAI GACNNNNGTC 1 cut(s) 173
PshBI ATTAAT 3 cut(s) 113, 903, 1547
PsiI TTATAA 1 cut(s) 2156
Psp5II RGGWCCY 1 cut(s) 2456
Psp6I CCWGG 3 cut(s) 7, 1212, 1371
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 3 cut(s) 7, 1212, 1371
PspN4I GGNNCC 8 cut(s) 6, 277, 685, 859, 893, 1726, 2014, 2457
PspPI GGNCC 2 cut(s) 892, 2456
PspPPI RGGWCCY 1 cut(s) 2456
PsrI GAACNNNNNNTAC 2 cut(s) 3036, 3068
PstI CTGCAG 1 cut(s) 1738
PstNI CAGNNNCTG 1 cut(s) 1241
PsuI RGATCY 2 cut(s) 857, 1122
PvuII CAGCTG 1 cut(s) 1739
RseI CAYNNNNRTG 2 cut(s) 1793, 1889
SapI GCTCTTC 2 cut(s) 1318, 1647
Sau96I GGNCC 2 cut(s) 892, 2456
ScaI AGTACT 1 cut(s) 2193
SchI GAGTC 4 cut(s) 606, 1823, 2456, 2474
ScrFI CCNGG 3 cut(s) 9, 1214, 1373
SduI GDGCHC 3 cut(s) 1501, 1971, 3028
SfcI CTRYAG 2 cut(s) 231, 1734
SfoI GGCGCC 1 cut(s) 1726
SinI GGWCC 1 cut(s) 2456
SmiMI CAYNNNNRTG 2 cut(s) 1793, 1889
SmlI CTYRAG 5 cut(s) 240, 1004, 1313, 2582, 2898
SmoI CTYRAG 5 cut(s) 240, 1004, 1313, 2582, 2898
SsiI CCGC 5 cut(s) 513, 633, 650, 2010, 3109
SspDI GGCGCC 1 cut(s) 1724
SspMI CTAG 9 cut(s) 42, 785, 941, 1107, 1446, 1502, 2147, 2445, 3029
StyD4I CCNGG 3 cut(s) 7, 1212, 1371
StyI CCWWGG 1 cut(s) 1627
TaiI ACGT 2 cut(s) 643, 3162
TaqI TCGA 8 cut(s) 340, 1074, 1266, 1482, 2492, 2531, 2558, 3009
TatI WGTACW 6 cut(s) 354, 1089, 2191, 2342, 2695, 3052
TfiI GAWTC 8 cut(s) 1134, 1224, 2441, 2503, 2528, 2789, 3038, 3185
TscAI CASTG 8 cut(s) 415, 613, 1209, 1623, 1881, 1896, 3076, 3208
TseFI GTSAC 2 cut(s) 316, 936
Tsp45I GTSAC 2 cut(s) 316, 936
TspDTI ATGAA 9 cut(s) 99, 802, 1345, 1818, 2288, 2872, 3027, 3209, 3224
TspGWI ACGGA 2 cut(s) 870, 961
TspRI CASTG 8 cut(s) 415, 613, 1209, 1623, 1881, 1896, 3076, 3208
Van91I CCANNNNNTGG 1 cut(s) 1241
VneI GTGCAC 2 cut(s) 1967, 3024
VpaK11BI GGWCC 1 cut(s) 2456
VspI ATTAAT 3 cut(s) 113, 903, 1547
XapI RAATTY 6 cut(s) 254, 1276, 1470, 1938, 2002, 2204
XbaI TCTAGA 1 cut(s) 1501
XceI RCATGY 3 cut(s) 633, 1148, 2591
XcmI CCANNNNNNNNNTGG 2 cut(s) 407, 1678
XmnI GAANNNNTTC 2 cut(s) 2775, 2793
XspI CTAG 9 cut(s) 42, 785, 941, 1107, 1446, 1502, 2147, 2445, 3029
ZraI GACGTC 1 cut(s) 3160
ZrmI AGTACT 1 cut(s) 2193
Zsp2I ATGCAT 2 cut(s) 970, 3220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.