Rw3G025470

Phytoene synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003237
Physical Location & Seq
Forward (+)
34077088 .. 34082344
5257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025470.1

Sequence Viewer

Length: 1245 bp
ATGTCTGTAGCATTGGTACGGGTGGTTTCCCCCAACACAAAGGTGTTAAATTGCTTTGGGCTGTTGGATTCATCAAGTTTTGTTTTGGGGGATGGAAGAGCAAAGATGGGTAGGAAGCAGAAACGGGATTCATGCTGTCTGAGTAGCACTTCTGGCTTACAAAGTGAAACCAAATTCCGCATTTTGTCAAGCATGGTAGCCAACCCGGCTGGAGAAATAGCAGTGTCATCGGAACAGAAGGTTTATGATGTGGTGCTGAAGCAGGCAGCCTTAGTGGAGAAGCAGTTGAGGACTAGTGGAGATGATGTTGATGTGAAGCCTGATATTATTCTTCCAGGGAATCTGAGCTTGTTGAGTGAAGCTTATGATAGATGCAGAGAAGTTTGTGCAGAGTATGCCAAGACTTTTTACCTGGGTACTCTGCTTATGACCCCTGAGAGAAGAAGAGCTATCTGGGCAATATATGTGTGGTGTAGAAGAACTGATGAGCTTGTTGATGGGCCTAATGCCTCACACATAACACCACAAGCTTTGGATAGGTGGGAGTCAAGGTTGGAAGATCTTTTCCAAGGTCGTCCATTCGATATGCTTGATGCTGCTTTATCAGATACAGTCACCCAATTTCCTGTTGACATTCAGCCATTCAAGGATATGATAGAGGGGATGAGAATGGACCTTAGGAAATCAATATACAAGAACTTTGATGAACTATACCTCTACTGTTATTATGTTGCCGGGACTGTTGGACTAATGAGTGTTCCAGTCATGGGCATTGCACCTGATTCGCAAGCAACTACAGAGTCTGTATATAATGCTGCCTTAGCATTGGGGATTGCGAATCAGCTAACTAACATACTAAGGGATGTTGGAGAAGATGCAAGGAGAGGACGCATTTATTTACCACAGGATGAGCTGGCACAAGCTGGACTTTCTGATGCTGACATATATGCTGGAAAGGTCACAGATAAATGGAGGAGTTTCATGCAGAGTCAGATTAAGAGAGCAAGGATGTTCTTTGATGAGGCAGAGAAAGGAGTCATGGAGCTCAGTGAAGCCAGCAGATGGCCGGTATTGGCGTCCCTACTTTTGTATCGCCAAATATTGGATGAGATAGAAGCTAATGACTACAACAACTTCACAAAAAGGGCTTATGTAAGCAAAGCTAAGAAATTACTTGCTTTGCCTATGGCATATACTAAATCCCTGATTCTGCCATCAAGAACACTTGAAATAAATCGAGCTTAG

Protein Analysis

414

Amino Acids

46.5

Weight (kDa)

6.13

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQS_PSY PF00494 131 - 388 3.9e-79 Squalene/phytoene synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1062, 1102
AciI CCGC 1 cut(s) 178
AcoI YGGCCR 1 cut(s) 1064
AcsI RAATTY 1 cut(s) 173
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 1076
AfaI GTAC 2 cut(s) 18, 418
AfiI CCNNNNNNNGG 3 cut(s) 767, 1062, 1102
AgsI TTSAA 2 cut(s) 646, 1229
AhlI ACTAGT 1 cut(s) 293
AjnI CCWGG 2 cut(s) 334, 411
AleI CACNNNNGTG 1 cut(s) 41
Alw21I GWGCWC 1 cut(s) 1047
AlwNI CAGNNNCTG 1 cut(s) 803
AoxI GGCC 2 cut(s) 500, 1064
ApeKI GCWGC 3 cut(s) 266, 596, 815
ApoI RAATTY 1 cut(s) 173
AspS9I GGNCC 2 cut(s) 500, 673
AsuC2I CCSGG 2 cut(s) 206, 736
AsuHPI GGTGA 1 cut(s) 607
AvaII GGWCC 1 cut(s) 673
AxyI CCTNAGG 1 cut(s) 677
BaeI ACNNNNGTAYC 2 cut(s) 1073, 1106
BanII GRGCYC 1 cut(s) 1047
Bbv12I GWGCWC 1 cut(s) 1047
BbvI GCAGC 3 cut(s) 278, 583, 802
BccI CCATC 5 cut(s) 86, 100, 491, 1056, 1222
BcgI CGANNNNNNTGC 4 cut(s) 210, 244, 765, 799
BciT130I CCWGG 2 cut(s) 336, 413
BcnI CCSGG 2 cut(s) 206, 736
BcuI ACTAGT 1 cut(s) 293
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 2 cut(s) 6, 795
BglI GCCNNNNNGGC 1 cut(s) 206
BglII AGATCT 1 cut(s) 559
BisI GCNGC 3 cut(s) 267, 597, 816
BlsI GCNGC 3 cut(s) 268, 598, 817
Bme1390I CCNGG 4 cut(s) 206, 336, 413, 736
Bme18I GGWCC 1 cut(s) 673
BmgT120I GGNCC 2 cut(s) 500, 673
BmrFI CCNGG 4 cut(s) 206, 336, 413, 736
BmsI GCATC 4 cut(s) 362, 583, 865, 925
BpmI CTGGAG 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 820
BpuMI CCSGG 2 cut(s) 206, 736
BsaHI GRCGYC 1 cut(s) 1076
BsaJI CCNNGG 3 cut(s) 335, 412, 568
BsaXI ACNNNNNCTCC 6 cut(s) 269, 291, 299, 321, 536, 566
Bsc4I CCNNNNNNNGG 3 cut(s) 767, 1062, 1102
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 1 cut(s) 761
Bse21I CCTNAGG 1 cut(s) 677
Bse3DI GCAATG 1 cut(s) 771
BseBI CCWGG 2 cut(s) 336, 413
BseDI CCNNGG 3 cut(s) 335, 412, 568
BseGI GGATG 6 cut(s) 97, 669, 868, 913, 1014, 1111
BseLI CCNNNNNNNGG 3 cut(s) 767, 1062, 1102
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 4 cut(s) 131, 335, 426, 1060
BseNI ACTGG 1 cut(s) 761
BseRI GAGGAG 1 cut(s) 988
BseXI GCAGC 3 cut(s) 278, 583, 802
BsgI GTGCAG 1 cut(s) 408
BshFI GGCC 2 cut(s) 502, 1066
BsiHKAI GWGCWC 1 cut(s) 1047
BsiSI CCGG 3 cut(s) 206, 735, 1067
BslFI GGGAC 2 cut(s) 751, 1063
BslI CCNNNNNNNGG 3 cut(s) 767, 1062, 1102
BsmFI GGGAC 2 cut(s) 751, 1063
BsnI GGCC 2 cut(s) 502, 1066
Bsp1286I GDGCHC 1 cut(s) 1047
Bsp143I GATC 1 cut(s) 559
BspACI CCGC 1 cut(s) 178
BspANI GGCC 2 cut(s) 502, 1066
BspCNI CTCAG 4 cut(s) 132, 336, 427, 1059
BspQI GCTCTTC 2 cut(s) 91, 439
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 1066
BsrI ACTGG 1 cut(s) 761
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 3 cut(s) 335, 412, 568
BssMI GATC 1 cut(s) 559
BssNI GRCGYC 1 cut(s) 1076
BssT1I CCWWGG 1 cut(s) 568
Bst2UI CCWGG 2 cut(s) 336, 413
Bst4CI ACNGT 3 cut(s) 613, 722, 742
Bst6I CTCTTC 2 cut(s) 91, 439
BstACI GRCGYC 1 cut(s) 1076
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 4 cut(s) 264, 789, 915, 1057
BstF5I GGATG 6 cut(s) 97, 669, 868, 913, 1014, 1111
BstKTI GATC 1 cut(s) 562
BstMBI GATC 1 cut(s) 559
BstMWI GCNNNNNNNGC 5 cut(s) 153, 206, 395, 455, 821
BstNI CCWGG 2 cut(s) 336, 413
BstSCI CCNGG 4 cut(s) 204, 334, 411, 734
BstSFI CTRYAG 2 cut(s) 6, 795
BstV1I GCAGC 3 cut(s) 278, 583, 802
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
Bsu36I CCTNAGG 1 cut(s) 677
BsuRI GGCC 2 cut(s) 502, 1066
BtsCI GGATG 6 cut(s) 97, 669, 868, 913, 1014, 1111
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 228, 1054
Cac8I GCNNGC 4 cut(s) 264, 789, 915, 1057
CaiI CAGNNNCTG 1 cut(s) 803
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 2 cut(s) 500, 673
CseI GACGC 2 cut(s) 897, 1065
Csp6I GTAC 2 cut(s) 17, 417
CspCI CAANNNNNGTGG 2 cut(s) 513, 548
CviAII CATG 5 cut(s) 132, 193, 766, 982, 1039
CviQI GTAC 2 cut(s) 17, 417
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
EaeI YGGCCR 1 cut(s) 1064
Eam1104I CTCTTC 2 cut(s) 91, 439
EarI CTCTTC 2 cut(s) 91, 439
Ecl136II GAGCTC 1 cut(s) 1045
Eco130I CCWWGG 1 cut(s) 568
Eco24I GRGCYC 1 cut(s) 1047
Eco47I GGWCC 1 cut(s) 673
Eco53kI GAGCTC 1 cut(s) 1045
Eco57I CTGAAG 1 cut(s) 278
Eco81I CCTNAGG 1 cut(s) 677
EcoICRI GAGCTC 1 cut(s) 1045
EcoRII CCWGG 2 cut(s) 334, 411
EcoT14I CCWWGG 1 cut(s) 568
EcoT38I GRGCYC 1 cut(s) 1047
ErhI CCWWGG 1 cut(s) 568
FaeI CATG 5 cut(s) 135, 196, 769, 985, 1042
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 2 cut(s) 751, 1063
FatI CATG 5 cut(s) 131, 192, 765, 981, 1038
Fnu4HI GCNGC 3 cut(s) 267, 597, 816
FokI GGATG 6 cut(s) 104, 676, 875, 920, 1021, 1118
FriOI GRGCYC 1 cut(s) 1047
Fsp4HI GCNGC 3 cut(s) 267, 597, 816
FspBI CTAG 1 cut(s) 294
GluI GCNGC 3 cut(s) 267, 597, 816
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 2 cut(s) 502, 1066
HapII CCGG 3 cut(s) 206, 735, 1067
HgaI GACGC 2 cut(s) 897, 1065
Hin1I GRCGYC 1 cut(s) 1076
Hin1II CATG 5 cut(s) 135, 196, 769, 985, 1042
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HindIII AAGCTT 2 cut(s) 360, 528
HpaII CCGG 3 cut(s) 206, 735, 1067
HphI GGTGA 1 cut(s) 607
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 6 cut(s) 141, 232, 345, 607, 934, 993
Hpy188III TCNNGA 1 cut(s) 1218
Hpy8I GTNNAC 1 cut(s) 631
HpyAV CCTTC 1 cut(s) 232
HpyCH4III ACNGT 3 cut(s) 613, 722, 742
HpyCH4V TGCA 5 cut(s) 375, 389, 776, 878, 985
HpyF10VI GCNNNNNNNGC 5 cut(s) 153, 206, 395, 455, 821
Hsp92I GRCGYC 1 cut(s) 1076
Hsp92II CATG 5 cut(s) 135, 196, 769, 985, 1042
Kzo9I GATC 1 cut(s) 559
LguI GCTCTTC 2 cut(s) 91, 439
LmnI GCTCC 1 cut(s) 1042
Lsp1109I GCAGC 3 cut(s) 278, 583, 802
LweI GCATC 4 cut(s) 362, 583, 865, 925
MaeI CTAG 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 613, 958
MalI GATC 1 cut(s) 561
MboI GATC 1 cut(s) 559
MboII GAAGA 7 cut(s) 108, 323, 453, 456, 489, 569, 884
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 1047
MluCI AATT 4 cut(s) 49, 173, 620, 1169
MlyI GAGTC 4 cut(s) 554, 809, 997, 1044
MmeI TCCRAC 4 cut(s) 45, 534, 724, 847
MnlI CCTC 7 cut(s) 282, 520, 652, 725, 878, 966, 1015
MseI TTAA 2 cut(s) 47, 996
MslI CAYNNNNRTG 1 cut(s) 41
MspI CCGG 3 cut(s) 206, 735, 1067
MspR9I CCNGG 4 cut(s) 206, 336, 413, 736
MvaI CCWGG 2 cut(s) 336, 413
MwoI GCNNNNNNNGC 5 cut(s) 153, 206, 395, 455, 821
NciI CCSGG 2 cut(s) 206, 736
NdeII GATC 1 cut(s) 559
NlaIII CATG 5 cut(s) 135, 196, 769, 985, 1042
NmuCI GTSAC 2 cut(s) 613, 958
OliI CACNNNNGTG 1 cut(s) 41
PciSI GCTCTTC 2 cut(s) 91, 439
PfeI GAWTC 6 cut(s) 68, 128, 340, 782, 838, 1207
PflMI CCANNNNNTGG 2 cut(s) 1062, 1102
PkrI GCNGC 3 cut(s) 268, 598, 817
PleI GAGTC 4 cut(s) 553, 808, 996, 1043
PpsI GAGTC 4 cut(s) 553, 808, 996, 1043
Psp124BI GAGCTC 1 cut(s) 1047
Psp6I CCWGG 2 cut(s) 334, 411
PspGI CCWGG 2 cut(s) 334, 411
PspPI GGNCC 2 cut(s) 500, 673
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 2 cut(s) 18, 418
RsaNI GTAC 2 cut(s) 17, 417
RseI CAYNNNNRTG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 1047
SapI GCTCTTC 2 cut(s) 91, 439
SaqAI TTAA 2 cut(s) 47, 996
SatI GCNGC 3 cut(s) 267, 597, 816
Sau3AI GATC 1 cut(s) 559
Sau96I GGNCC 2 cut(s) 500, 673
SchI GAGTC 4 cut(s) 554, 809, 997, 1044
ScrFI CCNGG 4 cut(s) 206, 336, 413, 736
SduI GDGCHC 1 cut(s) 1047
SfaNI GCATC 4 cut(s) 362, 583, 865, 925
SfcI CTRYAG 2 cut(s) 6, 795
SinI GGWCC 1 cut(s) 673
SmiMI CAYNNNNRTG 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 293
Sse9I AATT 4 cut(s) 49, 173, 620, 1169
SsiI CCGC 1 cut(s) 178
SspI AATATT 1 cut(s) 1101
SspMI CTAG 1 cut(s) 294
SstI GAGCTC 1 cut(s) 1047
StyD4I CCNGG 4 cut(s) 204, 334, 411, 734
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 3 cut(s) 613, 722, 742
TaqI TCGA 2 cut(s) 582, 1237
TasI AATT 4 cut(s) 49, 173, 620, 1169
TfiI GAWTC 6 cut(s) 68, 128, 340, 782, 838, 1207
Tru1I TTAA 2 cut(s) 47, 996
Tru9I TTAA 2 cut(s) 47, 996
TscAI CASTG 2 cut(s) 228, 1054
TseFI GTSAC 2 cut(s) 613, 958
TseI GCWGC 3 cut(s) 266, 596, 815
Tsp45I GTSAC 2 cut(s) 613, 958
TspDTI ATGAA 4 cut(s) 60, 120, 720, 970
TspRI CASTG 2 cut(s) 228, 1054
Van91I CCANNNNNTGG 2 cut(s) 1062, 1102
VpaK11BI GGWCC 1 cut(s) 673
XapI RAATTY 1 cut(s) 173
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.