FvH4_6g44380

Equilibrative nucleotide transporter 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008216
Physical Location & Seq
Forward (+)
34193495 .. 34197746
4252 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44380.t3

Sequence Viewer

Length: 1248 bp
ATGGCTGAACTTGATAATGGACAGCCACAGATTCAGGGGAAGTTTGCTGCAATGGTAGTCTGTTGGCTTCTGGGAAATGGGTGTCTCTTCTCATGGAACAGTATGCTTACAATCACCGATTACTATGATGCCTTGTTCCCGAGTTATCATCCGTCAAGGGTCCTCACTCTAGTATATCAGCCATTTGCGCTTTCTACACTCATAGTATTGTCATATCATGAAGCAAAGATCAATACTAGGAAGCGGAATTTATTTGGATATACACTGTTTTTCCTAAGTACTCTTTTGGTGCTAGTGTTGGATTTAGCTACATCAGGAAAAGGAGGGCTTGGAACTTACATTGGAATCTGTGCCATTAGTGGTGCTTTTGGAGTTGCAGATGCTCATGTGCAAGGAGGAATGATTGGAGACCTCTCCTTCATGAAACCAGAATTCATCCAATCCTTCCTTGCTGGTTTGGCTGCATCAGGGGCCCTAACCTCTGGACTGAGACTAGTTACCAAGGCAATATTTGAGAATTCCCAGAATGGTCTACGCAAGGGAGCCATTATGTTCTTTTCCATCTCTACACTCTTTGAGCTGCTATGTGTTTTCCTGTACGCTGTTGTCTTTCCAAAACTACCGATTGTGAAGTTCTACCGCTCCAAGGCAGCATCAGAAGGATCTAAAACAGTTTCGGCTGACCTTGCTGCTGGTGGCATCCAGACATTACCTGAAGGAGATGTGGAAGATCCGACTAAAATGCAGCGTCTGGGGAACAAGCAATTGCTATTTCAAAACATTGATTATGCAATTGATATGTTTCTAATCTATGCCCTGACCTTGTCAATTTTCCCCGGATTCCTATCAGAAGATACTGGATCTCACAGTTTGGGTGCATGGTATGCACTGGTTTTGATTGCGATGTACAATGTGTGGGATCTGATCGGGAGATACATTCCATGCTTGGATTGCCTCAAGTTAGAATCACGTAAATGGCTGATGGTTGCAATTGTCGCTCGTTTCTTACTTGTTCCGGCGTTCTATTTCACCGCCAATTATGGAGACCAAGGCTGGATGATCATGCTTACATCCTTCTTGGGTTTAACCAATGGTTACCTAACTGTTTGTGTGCTCACTTCAGCACCTAAAGGCTACAAGGGACCGGAGCAAAATGCTTTGGGAAATCTACTGGTAGTGTTCTTAATATTAGGTATCTTTGCAGGAGTAACACTCGACTGGTTGTGGCTTATAGGCAAGGGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

416

Amino Acids

45.56

Weight (kDa)

7.53

Isoelectric Point (pI)

29.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 124 - 409 4.7e-36 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 642
AccI GTMKAC 1 cut(s) 532
AciI CCGC 3 cut(s) 244, 640, 1032
AclWI GGATC 4 cut(s) 670, 725, 868, 927
AcsI RAATTY 3 cut(s) 247, 431, 517
AcuI CTGAAG 2 cut(s) 735, 1104
AfaI GTAC 3 cut(s) 280, 599, 908
AfiI CCNNNNNNNGG 1 cut(s) 646
AgsI TTSAA 1 cut(s) 776
AhlI ACTAGT 1 cut(s) 493
AluBI AGCT 2 cut(s) 308, 580
AluI AGCT 2 cut(s) 308, 580
Alw21I GWGCWC 1 cut(s) 1116
Alw26I GTCTC 4 cut(s) 89, 402, 484, 1038
AlwI GGATC 4 cut(s) 670, 725, 868, 927
AlwNI CAGNNNCTG 1 cut(s) 751
Ama87I CYCGRG 1 cut(s) 139
AoxI GGCC 1 cut(s) 471
ApaI GGGCCC 1 cut(s) 475
ApeKI GCWGC 6 cut(s) 47, 461, 580, 650, 689, 745
ApoI RAATTY 3 cut(s) 247, 431, 517
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 4 cut(s) 160, 471, 472, 1142
AsuC2I CCSGG 1 cut(s) 837
AsuHPI GGTGA 2 cut(s) 106, 1021
AvaI CYCGRG 1 cut(s) 139
AvaII GGWCC 2 cut(s) 160, 1142
BaeGI GKGCMC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 1116
BbvI GCAGC 6 cut(s) 34, 448, 567, 662, 676, 757
BccI CCATC 2 cut(s) 569, 976
BcgI CGANNNNNNTGC 2 cut(s) 724, 758
BclI TGATCA 1 cut(s) 1059
BcnI CCSGG 1 cut(s) 837
BcoDI GTCTC 4 cut(s) 89, 402, 484, 1038
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 4 cut(s) 170, 237, 293, 494
BisI GCNGC 6 cut(s) 48, 462, 581, 651, 690, 746
BlsI GCNGC 6 cut(s) 49, 463, 582, 652, 691, 747
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 1 cut(s) 837
Bme18I GGWCC 2 cut(s) 160, 1142
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 4 cut(s) 160, 471, 472, 1142
BmiI GGNNCC 5 cut(s) 161, 472, 473, 544, 1143
BmrFI CCNGG 1 cut(s) 837
BmsI GCATC 5 cut(s) 118, 370, 473, 662, 708
BplI GAGNNNNNCTC 2 cut(s) 1197, 1229
BpuEI CTTGAG 1 cut(s) 941
BpuMI CCSGG 1 cut(s) 837
BsaAI YACGTR 1 cut(s) 971
BsaBI GATNNNNATC 1 cut(s) 844
BsaI GGTCTC 2 cut(s) 402, 1038
BsaJI CCNNGG 4 cut(s) 501, 645, 835, 1048
BsaWI WCCGGW 1 cut(s) 1144
Bsc4I CCNNNNNNNGG 1 cut(s) 646
Bse1I ACTGG 4 cut(s) 862, 894, 1176, 1223
Bse3DI GCAATG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 844
BseDI CCNNGG 4 cut(s) 501, 645, 835, 1048
BseGI GGATG 5 cut(s) 148, 435, 699, 1062, 1070
BseJI GATNNNNATC 1 cut(s) 844
BseLI CCNNNNNNNGG 1 cut(s) 646
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 1 cut(s) 479
BseNI ACTGG 4 cut(s) 862, 894, 1176, 1223
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 6 cut(s) 34, 448, 567, 662, 676, 757
BshFI GGCC 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 1116
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 3 cut(s) 837, 1016, 1145
BslFI GGGAC 1 cut(s) 1155
BslI CCNNNNNNNGG 1 cut(s) 646
BsmAI GTCTC 4 cut(s) 89, 402, 484, 1038
BsmFI GGGAC 1 cut(s) 1155
BsnI GGCC 1 cut(s) 473
Bso31I GGTCTC 2 cut(s) 402, 1038
BsoBI CYCGRG 1 cut(s) 139
Bsp120I GGGCCC 1 cut(s) 471
Bsp1286I GDGCHC 2 cut(s) 475, 1116
Bsp1407I TGTACA 1 cut(s) 906
Bsp143I GATC 7 cut(s) 228, 662, 730, 860, 919, 924, 1059
BspACI CCGC 3 cut(s) 244, 640, 1032
BspANI GGCC 1 cut(s) 473
BspCNI CTCAG 1 cut(s) 480
BspHI TCATGA 2 cut(s) 217, 420
BspLI GGNNCC 5 cut(s) 161, 472, 473, 544, 1143
BspPI GGATC 4 cut(s) 670, 725, 868, 927
BspTNI GGTCTC 2 cut(s) 402, 1038
BsrBI CCGCTC 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 57
BsrGI TGTACA 1 cut(s) 906
BsrI ACTGG 4 cut(s) 862, 894, 1176, 1223
BssECI CCNNGG 4 cut(s) 501, 645, 835, 1048
BssMI GATC 7 cut(s) 228, 662, 730, 860, 919, 924, 1059
BssT1I CCWWGG 3 cut(s) 501, 645, 1048
Bst4CI ACNGT 5 cut(s) 101, 267, 673, 869, 1105
Bst6I CTCTTC 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 906
BstBAI YACGTR 1 cut(s) 971
BstDEI CTNAG 2 cut(s) 275, 488
BstEII GGTNACC 1 cut(s) 1094
BstF5I GGATG 5 cut(s) 148, 435, 699, 1062, 1070
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 7 cut(s) 231, 665, 733, 863, 922, 927, 1062
BstMAI GTCTC 4 cut(s) 89, 402, 484, 1038
BstMBI GATC 7 cut(s) 228, 662, 730, 860, 919, 924, 1059
BstMWI GCNNNNNNNGC 7 cut(s) 187, 458, 470, 686, 884, 951, 995
BstPI GGTNACC 1 cut(s) 1094
BstSCI CCNGG 1 cut(s) 835
BstSLI GKGCMC 1 cut(s) 475
BstV1I GCAGC 6 cut(s) 34, 448, 567, 662, 676, 757
BstX2I RGATCY 4 cut(s) 662, 730, 860, 919
BstYI RGATCY 4 cut(s) 662, 730, 860, 919
BsuRI GGCC 1 cut(s) 473
BtgZI GCGATG 1 cut(s) 917
BtsCI GGATG 5 cut(s) 148, 435, 699, 1062, 1070
BtsIMutI CAGTG 2 cut(s) 263, 887
CaiI CAGNNNCTG 1 cut(s) 751
CciI TCATGA 2 cut(s) 217, 420
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 4 cut(s) 160, 471, 472, 1142
CseI GACGC 1 cut(s) 737
Csp6I GTAC 3 cut(s) 279, 598, 907
CviAII CATG 7 cut(s) 93, 218, 386, 421, 879, 942, 1063
CviQI GTAC 3 cut(s) 279, 598, 907
DdeI CTNAG 2 cut(s) 275, 488
DpnI GATC 7 cut(s) 230, 664, 732, 862, 921, 926, 1061
DpnII GATC 7 cut(s) 228, 662, 730, 860, 919, 924, 1059
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco130I CCWWGG 3 cut(s) 501, 645, 1048
Eco24I GRGCYC 1 cut(s) 475
Eco31I GGTCTC 2 cut(s) 402, 1038
Eco47I GGWCC 2 cut(s) 160, 1142
Eco57I CTGAAG 2 cut(s) 735, 1104
Eco88I CYCGRG 1 cut(s) 139
Eco91I GGTNACC 1 cut(s) 1094
EcoO109I RGGNCCY 3 cut(s) 160, 471, 472
EcoO65I GGTNACC 1 cut(s) 1094
EcoRI GAATTC 2 cut(s) 431, 517
EcoT14I CCWWGG 3 cut(s) 501, 645, 1048
EcoT38I GRGCYC 1 cut(s) 475
ErhI CCWWGG 3 cut(s) 501, 645, 1048
FaeI CATG 7 cut(s) 96, 221, 389, 424, 882, 945, 1066
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 1 cut(s) 1155
FatI CATG 7 cut(s) 92, 217, 385, 420, 878, 941, 1062
FbaI TGATCA 1 cut(s) 1059
FblI GTMKAC 1 cut(s) 532
Fnu4HI GCNGC 6 cut(s) 48, 462, 581, 651, 690, 746
FokI GGATG 5 cut(s) 135, 422, 686, 1057, 1069
FriOI GRGCYC 1 cut(s) 475
Fsp4HI GCNGC 6 cut(s) 48, 462, 581, 651, 690, 746
FspBI CTAG 4 cut(s) 170, 237, 293, 494
GlaI GCGC 1 cut(s) 189
GluI GCNGC 6 cut(s) 48, 462, 581, 651, 690, 746
HaeIII GGCC 1 cut(s) 473
HapII CCGG 3 cut(s) 837, 1016, 1145
HgaI GACGC 1 cut(s) 737
HhaI GCGC 1 cut(s) 190
Hin1II CATG 7 cut(s) 96, 221, 389, 424, 882, 945, 1066
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 4 cut(s) 31, 345, 840, 965
HpaII CCGG 3 cut(s) 837, 1016, 1145
HphI GGTGA 2 cut(s) 106, 1021
Hpy166II GTNNAC 1 cut(s) 533
Hpy188I TCNGA 4 cut(s) 658, 735, 850, 924
Hpy188III TCNNGA 7 cut(s) 139, 218, 315, 421, 483, 703, 928
Hpy8I GTNNAC 1 cut(s) 533
HpyAV CCTTC 5 cut(s) 427, 454, 653, 710, 1084
HpyCH4III ACNGT 5 cut(s) 101, 267, 673, 869, 1105
HpyCH4IV ACGT 1 cut(s) 970
HpyF10VI GCNNNNNNNGC 7 cut(s) 187, 458, 470, 686, 884, 951, 995
HpyF3I CTNAG 2 cut(s) 275, 488
HpySE526I ACGT 1 cut(s) 970
Hsp92II CATG 7 cut(s) 96, 221, 389, 424, 882, 945, 1066
HspAI GCGC 1 cut(s) 188
Ksp22I TGATCA 1 cut(s) 1059
Kzo9I GATC 7 cut(s) 228, 662, 730, 860, 919, 924, 1059
LmnI GCTCC 3 cut(s) 542, 647, 1147
Lsp1109I GCAGC 6 cut(s) 34, 448, 567, 662, 676, 757
LweI GCATC 5 cut(s) 118, 370, 473, 662, 708
MaeI CTAG 4 cut(s) 170, 237, 293, 494
MaeII ACGT 1 cut(s) 970
MaeIII GTNAC 3 cut(s) 496, 1094, 1207
MalI GATC 7 cut(s) 230, 664, 732, 862, 921, 926, 1061
MbiI CCGCTC 1 cut(s) 642
MboI GATC 7 cut(s) 228, 662, 730, 860, 919, 924, 1059
MboII GAAGA 3 cut(s) 79, 740, 863
MfeI CAATTG 3 cut(s) 764, 792, 990
MflI RGATCY 4 cut(s) 662, 730, 860, 919
MhlI GDGCHC 2 cut(s) 475, 1116
MluCI AATT 8 cut(s) 247, 431, 517, 764, 792, 828, 990, 1036
MmeI TCCRAC 2 cut(s) 279, 758
MnlI CCTC 6 cut(s) 173, 317, 389, 422, 490, 965
MseI TTAA 2 cut(s) 1085, 1184
MslI CAYNNNNRTG 1 cut(s) 973
MspI CCGG 3 cut(s) 837, 1016, 1145
MspR9I CCNGG 1 cut(s) 837
MunI CAATTG 3 cut(s) 764, 792, 990
MwoI GCNNNNNNNGC 7 cut(s) 187, 458, 470, 686, 884, 951, 995
NciI CCSGG 1 cut(s) 837
NdeII GATC 7 cut(s) 228, 662, 730, 860, 919, 924, 1059
NlaIII CATG 7 cut(s) 96, 221, 389, 424, 882, 945, 1066
NlaIV GGNNCC 5 cut(s) 161, 472, 473, 544, 1143
PagI TCATGA 2 cut(s) 217, 420
PfeI GAWTC 4 cut(s) 31, 345, 840, 965
PflFI GACNNNGTC 1 cut(s) 823
PkrI GCNGC 6 cut(s) 49, 463, 582, 652, 691, 747
Ppu21I YACGTR 1 cut(s) 971
PpuMI RGGWCCY 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 160
PspEI GGTNACC 1 cut(s) 1094
PspN4I GGNNCC 5 cut(s) 161, 472, 473, 544, 1143
PspOMI GGGCCC 1 cut(s) 471
PspPI GGNCC 4 cut(s) 160, 471, 472, 1142
PspPPI RGGWCCY 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 751
PsuI RGATCY 4 cut(s) 662, 730, 860, 919
PsyI GACNNNGTC 1 cut(s) 823
RsaI GTAC 3 cut(s) 280, 599, 908
RsaNI GTAC 3 cut(s) 279, 598, 907
RseI CAYNNNNRTG 1 cut(s) 973
SaqAI TTAA 2 cut(s) 1085, 1184
SatI GCNGC 6 cut(s) 48, 462, 581, 651, 690, 746
Sau3AI GATC 7 cut(s) 228, 662, 730, 860, 919, 924, 1059
Sau96I GGNCC 4 cut(s) 160, 471, 472, 1142
ScaI AGTACT 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 837
SduI GDGCHC 2 cut(s) 475, 1116
SfaNI GCATC 5 cut(s) 118, 370, 473, 662, 708
SinI GGWCC 2 cut(s) 160, 1142
SmiMI CAYNNNNRTG 1 cut(s) 973
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 8 cut(s) 247, 431, 517, 764, 792, 828, 990, 1036
SsiI CCGC 3 cut(s) 244, 640, 1032
SspI AATATT 2 cut(s) 510, 1188
SspMI CTAG 4 cut(s) 170, 237, 293, 494
StyD4I CCNGG 1 cut(s) 835
StyI CCWWGG 3 cut(s) 501, 645, 1048
TaaI ACNGT 5 cut(s) 101, 267, 673, 869, 1105
TaiI ACGT 1 cut(s) 973
TaqI TCGA 1 cut(s) 1215
TasI AATT 8 cut(s) 247, 431, 517, 764, 792, 828, 990, 1036
TatI WGTACW 2 cut(s) 278, 906
TfiI GAWTC 4 cut(s) 31, 345, 840, 965
Tru1I TTAA 2 cut(s) 1085, 1184
Tru9I TTAA 2 cut(s) 1085, 1184
TscAI CASTG 2 cut(s) 270, 894
TseI GCWGC 6 cut(s) 47, 461, 580, 650, 689, 745
TspDTI ATGAA 4 cut(s) 234, 409, 424, 437
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 270, 894
Tth111I GACNNNGTC 1 cut(s) 823
VpaK11BI GGWCC 2 cut(s) 160, 1142
XapI RAATTY 3 cut(s) 247, 431, 517
XmiI GTMKAC 1 cut(s) 532
XspI CTAG 4 cut(s) 170, 237, 293, 494
ZrmI AGTACT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.