Rorug02G0488400

Equilibrative nucleotide transporter 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008216
Physical Location & Seq
Forward (+)
61820068 .. 61820728
661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0488400.1

Sequence Viewer

Length: 573 bp
ATGGCACTGGAAGAAGGAGATTCATTATTCAGTTCACTGAAACTACAACAACAAGAAGAACAAAGAGAGAGGCTGATTGTAGTGAGCGGTCCTTGCAAGGTGGTTGAGTATTTGCAGCCAGTAATGTCAAAAGAGCTTCTCTGTAAGTTCCCAGACAACTCTGCCTTCGATTTCGACTACACGCAGAGCTCGATATGGTCTCCGCTGGTCCCGCGGGCTTACGCTCCGATGGATTTGGACTTCGATTTCGATTTCATGACCCCGAGAAAGCTCACATTTGATCAAGTGGAGTTGGAGTCCAACAATCAAAGTAGCAGCATCAAGAAAGTGGGTTCTAGTGGTAAGAAGAAGATGTGCACTGCTGGGTTTAATCTTAACTTGAGTGCATTGAAGAAGAAGAAGTGGAAAAGCAAGAAGAACAAGATGGCATTGGTACCTGATTTCTCTCCAACTCCTGTTAAGGGTAATTGTTACCCCATTGCCTCAAAGGTACTGCCAGATTTTCTGGGTTATGTAGATCTTTGCTTTAGTATAGTTTATTTGTGTTATTTCAATAAATTGAGCTCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

190

Amino Acids

21.58

Weight (kDa)

8.64

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 433
AccB1I GGYRCC 1 cut(s) 433
AccBSI CCGCTC 1 cut(s) 87
AccII CGCG 1 cut(s) 214
AciI CCGC 4 cut(s) 87, 203, 212, 214
AfaI GTAC 2 cut(s) 435, 492
AfiI CCNNNNNNNGG 1 cut(s) 461
AgsI TTSAA 2 cut(s) 391, 553
AluBI AGCT 4 cut(s) 136, 189, 271, 564
AluI AGCT 4 cut(s) 136, 189, 271, 564
Alw21I GWGCWC 3 cut(s) 191, 359, 566
Alw26I GTCTC 1 cut(s) 204
Alw44I GTGCAC 1 cut(s) 355
Ama87I CYCGRG 1 cut(s) 262
ApaLI GTGCAC 1 cut(s) 355
ApeKI GCWGC 2 cut(s) 115, 315
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
Asp718I GGTACC 1 cut(s) 433
AspS9I GGNCC 2 cut(s) 89, 208
AvaI CYCGRG 1 cut(s) 262
AvaII GGWCC 2 cut(s) 89, 208
BaeGI GKGCMC 1 cut(s) 359
BanI GGYRCC 1 cut(s) 433
BanII GRGCYC 2 cut(s) 191, 566
Bbv12I GWGCWC 3 cut(s) 191, 359, 566
BbvI GCAGC 2 cut(s) 127, 327
BccI CCATC 2 cut(s) 223, 418
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 1 cut(s) 336
BglII AGATCT 1 cut(s) 517
BisI GCNGC 2 cut(s) 116, 316
BlsI GCNGC 2 cut(s) 117, 317
Bme18I GGWCC 2 cut(s) 89, 208
BmeT110I CYCGRG 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 89, 208
BmiI GGNNCC 2 cut(s) 210, 435
BmsI GCATC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 400
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse1I ACTGG 2 cut(s) 12, 119
Bse3DI GCAATG 1 cut(s) 477
BseDI CCNNGG 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 461
BseMI GCAATG 1 cut(s) 477
BseNI ACTGG 2 cut(s) 12, 119
BseSI GKGCMC 1 cut(s) 359
BseXI GCAGC 2 cut(s) 127, 327
BseYI CCCAGC 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 214
BshNI GGYRCC 1 cut(s) 433
BsiHKAI GWGCWC 3 cut(s) 191, 359, 566
BsiHKCI CYCGRG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 461
BsmAI GTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 194
Bso31I GGTCTC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 262
Bsp1286I GDGCHC 3 cut(s) 191, 359, 566
Bsp143I GATC 2 cut(s) 280, 517
BspACI CCGC 4 cut(s) 87, 203, 212, 214
BspFNI CGCG 1 cut(s) 214
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 210, 435
BspT107I GGYRCC 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 204
BsrBI CCGCTC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 477
BsrI ACTGG 2 cut(s) 12, 119
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 2 cut(s) 280, 517
BstC8I GCNNGC 1 cut(s) 216
BstDSI CCRYGG 1 cut(s) 212
BstFNI CGCG 1 cut(s) 214
BstKTI GATC 2 cut(s) 283, 520
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 2 cut(s) 280, 517
BstMWI GCNNNNNNNGC 2 cut(s) 93, 211
BstSLI GKGCMC 1 cut(s) 359
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 127, 327
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BtgI CCRYGG 1 cut(s) 212
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 3 cut(s) 5, 35, 357
Cac8I GCNNGC 1 cut(s) 216
CciI TCATGA 1 cut(s) 255
Cfr13I GGNCC 2 cut(s) 89, 208
Cfr42I CCGCGG 1 cut(s) 215
Csp6I GTAC 2 cut(s) 434, 491
CviAII CATG 1 cut(s) 256
CviJI RGCY 7 cut(s) 73, 118, 136, 189, 218, 271, 564
CviKI_1 RGCY 7 cut(s) 73, 118, 136, 189, 218, 271, 564
CviQI GTAC 2 cut(s) 434, 491
DpnI GATC 2 cut(s) 282, 519
DpnII GATC 2 cut(s) 280, 517
Ecl136II GAGCTC 2 cut(s) 189, 564
Eco24I GRGCYC 2 cut(s) 191, 566
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 89, 208
Eco53kI GAGCTC 2 cut(s) 189, 564
Eco88I CYCGRG 1 cut(s) 262
EcoICRI GAGCTC 2 cut(s) 189, 564
EcoT38I GRGCYC 2 cut(s) 191, 566
FaeI CATG 1 cut(s) 259
FaiI YATR 4 cut(s) 196, 257, 513, 533
FaqI GGGAC 1 cut(s) 194
FatI CATG 1 cut(s) 255
FauI CCCGC 2 cut(s) 207, 219
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 2 cut(s) 116, 316
FriOI GRGCYC 2 cut(s) 191, 566
Fsp4HI GCNGC 2 cut(s) 116, 316
FspBI CTAG 1 cut(s) 336
GluI GCNGC 2 cut(s) 116, 316
GsaI CCCAGC 1 cut(s) 366
Hin1II CATG 1 cut(s) 259
HinfI GANTC 2 cut(s) 20, 296
Hpy166II GTNNAC 2 cut(s) 35, 357
Hpy188I TCNGA 1 cut(s) 228
Hpy188III TCNNGA 2 cut(s) 256, 322
Hpy8I GTNNAC 2 cut(s) 35, 357
HpyAV CCTTC 2 cut(s) 8, 175
HpyCH4V TGCA 4 cut(s) 96, 115, 357, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 211
Hsp92II CATG 1 cut(s) 259
KpnI GGTACC 1 cut(s) 437
Ksp22I TGATCA 1 cut(s) 280
KspI CCGCGG 1 cut(s) 215
Kzo9I GATC 2 cut(s) 280, 517
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 8 cut(s) 132, 165, 191, 348, 450, 468, 491, 510
Lsp1109I GCAGC 2 cut(s) 127, 327
LweI GCATC 1 cut(s) 327
MaeI CTAG 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 470
MalI GATC 2 cut(s) 282, 519
MbiI CCGCTC 1 cut(s) 87
MboI GATC 2 cut(s) 280, 517
MboII GAAGA 8 cut(s) 23, 68, 358, 361, 403, 406, 409, 427
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 3 cut(s) 191, 359, 566
MluCI AATT 2 cut(s) 466, 557
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 3 cut(s) 273, 324, 473
MnlI CCTC 2 cut(s) 63, 493
MseI TTAA 3 cut(s) 369, 375, 459
MspA1I CMGCKG 2 cut(s) 205, 214
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 2 cut(s) 93, 211
NdeII GATC 2 cut(s) 280, 517
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 210, 435
PagI TCATGA 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 2 cut(s) 117, 317
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Psp124BI GAGCTC 2 cut(s) 191, 566
PspFI CCCAGC 1 cut(s) 362
PspN4I GGNNCC 2 cut(s) 210, 435
PspPI GGNCC 2 cut(s) 89, 208
PsuI RGATCY 1 cut(s) 517
RsaI GTAC 2 cut(s) 435, 492
RsaNI GTAC 2 cut(s) 434, 491
SacI GAGCTC 2 cut(s) 191, 566
SacII CCGCGG 1 cut(s) 215
SaqAI TTAA 3 cut(s) 369, 375, 459
SatI GCNGC 2 cut(s) 116, 316
Sau3AI GATC 2 cut(s) 280, 517
Sau96I GGNCC 2 cut(s) 89, 208
SchI GAGTC 1 cut(s) 305
SduI GDGCHC 3 cut(s) 191, 359, 566
SetI ASST 7 cut(s) 102, 138, 191, 273, 439, 492, 566
SfaNI GCATC 1 cut(s) 327
Sfr303I CCGCGG 1 cut(s) 215
SgrBI CCGCGG 1 cut(s) 215
SinI GGWCC 2 cut(s) 89, 208
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse9I AATT 2 cut(s) 466, 557
SsiI CCGC 4 cut(s) 87, 203, 212, 214
SspMI CTAG 1 cut(s) 336
SstI GAGCTC 2 cut(s) 191, 566
TaqI TCGA 5 cut(s) 168, 174, 191, 243, 249
TasI AATT 2 cut(s) 466, 557
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 3 cut(s) 369, 375, 459
Tru9I TTAA 3 cut(s) 369, 375, 459
TscAI CASTG 3 cut(s) 12, 42, 364
TseI GCWGC 2 cut(s) 115, 315
TspDTI ATGAA 2 cut(s) 12, 244
TspRI CASTG 3 cut(s) 12, 42, 364
VneI GTGCAC 1 cut(s) 355
VpaK11BI GGWCC 2 cut(s) 89, 208
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.