MD09G1097900.v1.1

Equilibrative nucleotide transporter 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008216
Physical Location & Seq
Reverse (-)
7121837 .. 7125772
3936 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1097900.v1.1.491

Sequence Viewer

Length: 1251 bp
ATGGCAAGCCCTGATGCACCCCCACCAAAGCTTGAGGGGAAGTATGCTGCAATGGTAGTGTGTTGGTTTCTTGGAAATGGATGCCTCTTTTCGTGGAACAGCATGATCACAATTGTAGATTACTATGTTTACTTGTTCCCGTGGTACCATCCATCGAGGGTACTTACAGTGGTATACCAGCCATTCGCAGTTTTAACGATCATCGTACTGTCATACCATGAGGCGAAGATCAATACGAGGAAGCGAAACCTGTTAGGATACATTCTGTTTTTCCTAAGTTCTCTGTTGGTGCTAGTTTTGGATTTAGCCACATCCGGGAAAGGAGGTCTTGGAACTTTTATCGGAATTTGTGCAATTAGCGCTGCCTTTGGAATTGCAGATGCTCATGTGCTAGGAGGAATGGTTGGAGACCTCTCTTTCATGCCAGGAGAGCTCATCCAATCTTTCCTTGCTGGTTTGGCTGCCTCAGGAGCTCTAACCTCTGCTTTGAGGTTGATTACAAAGGCGGCATTTGAGAATTCCCAAAATGGTCTTAGAAAGGGAGCCATTTTGTTCTTTGCCATCACTTCATTCTTCGAGCTTCTCTGCATTCTCCTCTACGCTTTTTCTTTTCCGAAACTGCCAATTGTTAAGTACTATCGTTCTAAGGCTGCTTCCGAAGGATCTAAAACTGTTTCAGCTGACCTTGCCGCTGGTGGCATCCAAACATTGGCTGAAGCAACAGATGAGGAGAATCCTAGAGAACCTAATCGACTTGGAAACAAACAGTTGTTGCTTCAAAACATTGATTATGCACTTGACATATTTCTAATCTATACGCTCACTTTGTCAATTTCCCCCGGATTCTTATCAGAAGACACTGGATCTCACAGTTTGGGAACATGGTATGCACTTGTTTTGATTGCACTGTACAATGTGTGTGATCTCATCGGAAGATACATTCCGCTAGTGAAATTCCTGAAGTTGGAATCGCGGAGAGGGCTCATGACCGTGATTCTTTCGCGCTTCTTACTTGTCCCGTGTTTCTACTTCACAGCCAAGTATGGTGAACAAGGCTGGATGATCATGCTCACATCCTTCTTAGGTTTAACCAATGGTTACCTCACCGTCTGCGTCCTTACTTCAGCACCTAAGGGCTACAGGGGGCCGGAGCAAAATGCTTTGGGAAATCTTCTGGTGTTGTTCTTGCTTGGAGGTATACTAGCAGGGGTGTTACTTGACTGGTTGTGGCTTATAGGCAAGGGCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

417

Amino Acids

45.56

Weight (kDa)

8.54

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 124 - 410 2.3e-35 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 709
AccI GTMKAC 2 cut(s) 174, 1198
AccII CGCG 2 cut(s) 973, 1003
AciI CCGC 4 cut(s) 506, 690, 944, 973
AclWI GGATC 2 cut(s) 670, 871
AcsI RAATTY 3 cut(s) 345, 517, 953
AcuI CTGAAG 3 cut(s) 735, 980, 1107
AfaI GTAC 5 cut(s) 146, 162, 207, 635, 911
AfeI AGCGCT 1 cut(s) 361
AfiI CCNNNNNNNGG 3 cut(s) 315, 709, 964
AgsI TTSAA 1 cut(s) 779
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 5 cut(s) 31, 433, 473, 580, 680
AluI AGCT 5 cut(s) 31, 433, 473, 580, 680
Alw21I GWGCWC 2 cut(s) 435, 475
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 2 cut(s) 670, 871
Aor51HI AGCGCT 1 cut(s) 361
AoxI GGCC 1 cut(s) 1145
ApeKI GCWGC 4 cut(s) 47, 362, 461, 650
ApoI RAATTY 3 cut(s) 345, 517, 953
Asp718I GGTACC 1 cut(s) 144
AspLEI GCGC 2 cut(s) 362, 1005
AspS9I GGNCC 1 cut(s) 1145
AsuC2I CCSGG 2 cut(s) 316, 840
AsuHPI GGTGA 2 cut(s) 1058, 1096
AxyI CCTNAGG 2 cut(s) 466, 1131
BanI GGYRCC 1 cut(s) 144
BanII GRGCYC 3 cut(s) 435, 475, 984
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 2 cut(s) 435, 475
BbvI GCAGC 4 cut(s) 34, 349, 448, 637
BccI CCATC 3 cut(s) 156, 160, 569
BciT130I CCWGG 1 cut(s) 426
BciVI GTATCC 1 cut(s) 251
BclI TGATCA 2 cut(s) 105, 1062
BcnI CCSGG 2 cut(s) 316, 840
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 5 cut(s) 293, 392, 738, 947, 1202
BfmI CTRYAG 1 cut(s) 1138
BfoI RGCGCY 1 cut(s) 363
BfuI GTATCC 1 cut(s) 251
BisI GCNGC 6 cut(s) 48, 363, 462, 507, 651, 690
BlsI GCNGC 6 cut(s) 49, 364, 463, 508, 652, 691
BmcAI AGTACT 1 cut(s) 635
Bme1390I CCNGG 3 cut(s) 316, 426, 840
BmgT120I GGNCC 1 cut(s) 1145
BmiI GGNNCC 3 cut(s) 146, 544, 1146
BmrFI CCNGG 3 cut(s) 316, 426, 840
BmsI GCATC 4 cut(s) 4, 71, 370, 708
BpiI GAAGAC 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 2 cut(s) 316, 840
BsaBI GATNNNNATC 1 cut(s) 847
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 2 cut(s) 140, 838
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bsc4I CCNNNNNNNGG 3 cut(s) 315, 709, 964
Bse1I ACTGG 2 cut(s) 865, 1226
Bse21I CCTNAGG 2 cut(s) 466, 1131
Bse3DI GCAATG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 847
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 140, 838
BseGI GGATG 7 cut(s) 86, 148, 311, 435, 699, 1065, 1073
BseJI GATNNNNATC 1 cut(s) 847
BseLI CCNNNNNNNGG 3 cut(s) 315, 709, 964
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 2 cut(s) 865, 1226
BseRI GAGGAG 2 cut(s) 584, 743
BseXI GCAGC 4 cut(s) 34, 349, 448, 637
Bsh1236I CGCG 2 cut(s) 973, 1003
BshFI GGCC 1 cut(s) 1147
BshNI GGYRCC 1 cut(s) 144
BsiHKAI GWGCWC 2 cut(s) 435, 475
BsiSI CCGG 3 cut(s) 315, 840, 1148
BslFI GGGAC 1 cut(s) 1001
BslI CCNNNNNNNGG 3 cut(s) 315, 709, 964
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 1001
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 1 cut(s) 1147
Bso31I GGTCTC 1 cut(s) 402
Bsp1286I GDGCHC 3 cut(s) 435, 475, 984
Bsp1407I TGTACA 1 cut(s) 909
Bsp143I GATC 7 cut(s) 105, 198, 228, 662, 863, 922, 1062
BspACI CCGC 4 cut(s) 506, 690, 944, 973
BspANI GGCC 1 cut(s) 1147
BspCNI CTCAG 1 cut(s) 479
BspFNI CGCG 2 cut(s) 973, 1003
BspHI TCATGA 1 cut(s) 984
BspLI GGNNCC 3 cut(s) 146, 544, 1146
BspPI GGATC 2 cut(s) 670, 871
BspT107I GGYRCC 1 cut(s) 144
BspTNI GGTCTC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 57
BsrGI TGTACA 1 cut(s) 909
BsrI ACTGG 2 cut(s) 865, 1226
BssECI CCNNGG 2 cut(s) 140, 838
BssMI GATC 7 cut(s) 105, 198, 228, 662, 863, 922, 1062
BssNAI GTATAC 2 cut(s) 175, 1199
Bst1107I GTATAC 2 cut(s) 175, 1199
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 8 cut(s) 169, 210, 673, 768, 872, 909, 991, 1108
BstAUI TGTACA 1 cut(s) 909
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 6 cut(s) 275, 466, 533, 645, 1081, 1131
BstDSI CCRYGG 1 cut(s) 140
BstEII GGTNACC 1 cut(s) 1097
BstF5I GGATG 7 cut(s) 86, 148, 311, 435, 699, 1065, 1073
BstFNI CGCG 2 cut(s) 973, 1003
BstH2I RGCGCY 1 cut(s) 363
BstHHI GCGC 2 cut(s) 362, 1005
BstKTI GATC 7 cut(s) 108, 201, 231, 665, 866, 925, 1065
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 7 cut(s) 105, 198, 228, 662, 863, 922, 1062
BstMWI GCNNNNNNNGC 6 cut(s) 359, 430, 458, 470, 686, 979
BstNI CCWGG 1 cut(s) 426
BstPI GGTNACC 1 cut(s) 1097
BstSCI CCNGG 3 cut(s) 314, 424, 838
BstSFI CTRYAG 1 cut(s) 1138
BstUI CGCG 2 cut(s) 973, 1003
BstV1I GCAGC 4 cut(s) 34, 349, 448, 637
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 2 cut(s) 662, 863
BstYI RGATCY 2 cut(s) 662, 863
BstZ17I GTATAC 2 cut(s) 175, 1199
Bsu36I CCTNAGG 2 cut(s) 466, 1131
BsuI GTATCC 1 cut(s) 251
BsuRI GGCC 1 cut(s) 1147
BtgI CCRYGG 1 cut(s) 140
BtsCI GGATG 7 cut(s) 86, 148, 311, 435, 699, 1065, 1073
BtsIMutI CAGTG 3 cut(s) 174, 858, 905
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 984
CfoI GCGC 2 cut(s) 362, 1005
Cfr13I GGNCC 1 cut(s) 1145
CseI GACGC 1 cut(s) 1102
Csp6I GTAC 5 cut(s) 145, 161, 206, 634, 910
CviAII CATG 7 cut(s) 103, 218, 386, 421, 882, 985, 1066
CviQI GTAC 5 cut(s) 145, 161, 206, 634, 910
DdeI CTNAG 6 cut(s) 275, 466, 533, 645, 1081, 1131
DpnI GATC 7 cut(s) 107, 200, 230, 664, 865, 924, 1064
DpnII GATC 7 cut(s) 105, 198, 228, 662, 863, 922, 1062
Ecl136II GAGCTC 2 cut(s) 433, 473
Eco24I GRGCYC 3 cut(s) 435, 475, 984
Eco31I GGTCTC 1 cut(s) 402
Eco47III AGCGCT 1 cut(s) 361
Eco53kI GAGCTC 2 cut(s) 433, 473
Eco57I CTGAAG 3 cut(s) 735, 980, 1107
Eco81I CCTNAGG 2 cut(s) 466, 1131
Eco91I GGTNACC 1 cut(s) 1097
EcoICRI GAGCTC 2 cut(s) 433, 473
EcoO65I GGTNACC 1 cut(s) 1097
EcoRI GAATTC 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 424
EcoT38I GRGCYC 3 cut(s) 435, 475, 984
FaeI CATG 7 cut(s) 106, 221, 389, 424, 885, 988, 1069
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 1 cut(s) 1001
FatI CATG 7 cut(s) 102, 217, 385, 420, 881, 984, 1065
FbaI TGATCA 2 cut(s) 105, 1062
FblI GTMKAC 2 cut(s) 174, 1198
Fnu4HI GCNGC 6 cut(s) 48, 363, 462, 507, 651, 690
FokI GGATG 7 cut(s) 93, 135, 298, 422, 686, 1060, 1072
FriOI GRGCYC 3 cut(s) 435, 475, 984
Fsp4HI GCNGC 6 cut(s) 48, 363, 462, 507, 651, 690
FspBI CTAG 5 cut(s) 293, 392, 738, 947, 1202
GlaI GCGC 2 cut(s) 361, 1004
GluI GCNGC 6 cut(s) 48, 363, 462, 507, 651, 690
HaeII RGCGCY 1 cut(s) 363
HaeIII GGCC 1 cut(s) 1147
HapII CCGG 3 cut(s) 315, 840, 1148
HgaI GACGC 1 cut(s) 1102
HhaI GCGC 2 cut(s) 362, 1005
Hin1II CATG 7 cut(s) 106, 221, 389, 424, 885, 988, 1069
Hin6I GCGC 2 cut(s) 360, 1003
HinP1I GCGC 2 cut(s) 360, 1003
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 4 cut(s) 733, 843, 968, 994
HpaII CCGG 3 cut(s) 315, 840, 1148
HphI GGTGA 2 cut(s) 1058, 1096
Hpy166II GTNNAC 4 cut(s) 130, 175, 1049, 1199
Hpy188I TCNGA 5 cut(s) 344, 615, 658, 853, 932
Hpy188III TCNNGA 3 cut(s) 468, 958, 985
Hpy8I GTNNAC 4 cut(s) 130, 175, 1049, 1199
HpyAV CCTTC 2 cut(s) 653, 1087
HpyCH4III ACNGT 8 cut(s) 169, 210, 673, 768, 872, 909, 991, 1108
HpyCH4V TGCA 8 cut(s) 17, 50, 353, 377, 588, 794, 890, 905
HpyF10VI GCNNNNNNNGC 6 cut(s) 359, 430, 458, 470, 686, 979
HpyF3I CTNAG 6 cut(s) 275, 466, 533, 645, 1081, 1131
Hsp92II CATG 7 cut(s) 106, 221, 389, 424, 885, 988, 1069
HspAI GCGC 2 cut(s) 360, 1003
KpnI GGTACC 1 cut(s) 148
Ksp22I TGATCA 2 cut(s) 105, 1062
Kzo9I GATC 7 cut(s) 105, 198, 228, 662, 863, 922, 1062
LmnI GCTCC 3 cut(s) 470, 542, 1150
Lsp1109I GCAGC 4 cut(s) 34, 349, 448, 637
LweI GCATC 4 cut(s) 4, 71, 370, 708
MaeI CTAG 5 cut(s) 293, 392, 738, 947, 1202
MaeIII GTNAC 2 cut(s) 1097, 1212
MalI GATC 7 cut(s) 107, 200, 230, 664, 865, 924, 1064
MboI GATC 7 cut(s) 105, 198, 228, 662, 863, 922, 1062
MboII GAAGA 5 cut(s) 238, 565, 866, 945, 1163
MfeI CAATTG 2 cut(s) 111, 624
MflI RGATCY 2 cut(s) 662, 863
MhlI GDGCHC 3 cut(s) 435, 475, 984
MluCI AATT 8 cut(s) 111, 345, 354, 372, 517, 624, 831, 953
MmeI TCCRAC 2 cut(s) 385, 945
MseI TTAA 3 cut(s) 194, 630, 1088
MslI CAYNNNNRTG 1 cut(s) 989
MspA1I CMGCKG 2 cut(s) 680, 692
MspI CCGG 3 cut(s) 315, 840, 1148
MspR9I CCNGG 3 cut(s) 316, 426, 840
MunI CAATTG 2 cut(s) 111, 624
Mva1269I GAATGC 1 cut(s) 588
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 2 cut(s) 973, 1003
MwoI GCNNNNNNNGC 6 cut(s) 359, 430, 458, 470, 686, 979
NciI CCSGG 2 cut(s) 316, 840
NdeII GATC 7 cut(s) 105, 198, 228, 662, 863, 922, 1062
NlaIII CATG 7 cut(s) 106, 221, 389, 424, 885, 988, 1069
NlaIV GGNNCC 3 cut(s) 146, 544, 1146
PagI TCATGA 1 cut(s) 984
PctI GAATGC 1 cut(s) 588
PfeI GAWTC 4 cut(s) 733, 843, 968, 994
PflMI CCANNNNNTGG 1 cut(s) 709
PfoI TCCNGGA 1 cut(s) 314
PkrI GCNGC 6 cut(s) 49, 364, 463, 508, 652, 691
Psp124BI GAGCTC 2 cut(s) 435, 475
Psp6I CCWGG 1 cut(s) 424
PspEI GGTNACC 1 cut(s) 1097
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 3 cut(s) 146, 544, 1146
PspPI GGNCC 1 cut(s) 1145
PsuI RGATCY 2 cut(s) 662, 863
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 5 cut(s) 146, 162, 207, 635, 911
RsaNI GTAC 5 cut(s) 145, 161, 206, 634, 910
RseI CAYNNNNRTG 1 cut(s) 989
SacI GAGCTC 2 cut(s) 435, 475
SaqAI TTAA 3 cut(s) 194, 630, 1088
SatI GCNGC 6 cut(s) 48, 363, 462, 507, 651, 690
Sau3AI GATC 7 cut(s) 105, 198, 228, 662, 863, 922, 1062
Sau96I GGNCC 1 cut(s) 1145
ScaI AGTACT 1 cut(s) 635
ScrFI CCNGG 3 cut(s) 316, 426, 840
SduI GDGCHC 3 cut(s) 435, 475, 984
SfaNI GCATC 4 cut(s) 4, 71, 370, 708
SfcI CTRYAG 1 cut(s) 1138
SmiMI CAYNNNNRTG 1 cut(s) 989
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 8 cut(s) 111, 345, 354, 372, 517, 624, 831, 953
SsiI CCGC 4 cut(s) 506, 690, 944, 973
SspMI CTAG 5 cut(s) 293, 392, 738, 947, 1202
SstI GAGCTC 2 cut(s) 435, 475
StyD4I CCNGG 3 cut(s) 314, 424, 838
TaaI ACNGT 8 cut(s) 169, 210, 673, 768, 872, 909, 991, 1108
TaqI TCGA 3 cut(s) 155, 576, 751
TasI AATT 8 cut(s) 111, 345, 354, 372, 517, 624, 831, 953
TatI WGTACW 2 cut(s) 633, 909
TauI GCSGC 2 cut(s) 509, 692
TfiI GAWTC 4 cut(s) 733, 843, 968, 994
Tru1I TTAA 3 cut(s) 194, 630, 1088
Tru9I TTAA 3 cut(s) 194, 630, 1088
TscAI CASTG 3 cut(s) 174, 865, 912
TseI GCWGC 4 cut(s) 47, 362, 461, 650
TspDTI ATGAA 2 cut(s) 409, 558
TspRI CASTG 3 cut(s) 174, 865, 912
Van91I CCANNNNNTGG 1 cut(s) 709
XapI RAATTY 3 cut(s) 345, 517, 953
XmiI GTMKAC 2 cut(s) 174, 1198
XspI CTAG 5 cut(s) 293, 392, 738, 947, 1202
ZrmI AGTACT 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.