Prupe.3G228300_v2.0.a1

Equilibrative nucleotide transporter 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0008216
Physical Location & Seq
Forward (+)
22725723 .. 22729547
3825 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G228300.1

Sequence Viewer

Length: 1254 bp
ATGGGAAGCCCTGATGTAAATAGACCCCCACCAAAGCTTGAGGGGAAGTATGCTGCAATGGTGGTGTGCTGGCTTCTGGGAAATGGATGCCTCTTTTCATGGAACAGTATGCTTACAATAGAAGATTACTATGTTGACTTGTTCCCGCGATACCATCCCGCAAGGGTCCTCACTCTGGTGTACCAGCCATTTGCGCTAGGAACAATTATAATCTTGTCGTATCATGAGGCGAAGATGAATACTAGGAAAAGAAACCTATTTGGATATGTCCTATTTTTCATAACTTCTCTATTGGTGCTAGTTTTGGATTTAGCTACATCTGGGAGAGGAGGCCTTGGAACTTTTATTGGAATTTGTGCCATTAGTGCTGCCTTTGGAATTGCAGATGCTCATGTGCAAGGAGGAATGGTTGGAGACCTCTCTTTCATGTCAGGAGAACTCATCCAATCTTTCCTTGCTGGTTTGGCTGCATCAGGAGCCCTAACCTCTGCTTTGAGGTTAATTACAAAGGCAGCATTTGAGAATTCCCATAATGGTCTTCGGAAAGGAGCTATTTTGTTCTTTGCAATCTCTTCGATCTTTGAGCTTCTCTGCGTGATTCTTTATGCTTTTGCCTTTCCGAAGCTACCAATTGTTAAGTACTATCGCTCAAAGGCCGCTTCAGAAGGATCAAAAACTGTTTCTGCTGACCTTGCTGCTGGTGGCATCCAAACATTGCCTGAAGCAGATGAGGAAGAGCAGAGAGATCTAGAGCGGCTGGGGAACAAACAATTGCTGCTTCAAAACATTGATTATGCACTCGATATGTTTCTAATCTATGCCCTCACTTTGTCAATTTTCCCTGGATTCTTATCCGAAGACACCGGATCACACAGCTTGGGTACATGGTATGCACTGGTTTTGATTGCAATGTACAATGTGTCTGATCTGATTGGGAGATACATTCCGCTGGTGAAATTCCTCAAGTTGGAAAATCGTAGAGGGCTCATGGTTGTGATTCTTTCTCGTTTCTTGCTCGTCCCGGCATTCTATTTCACTGCCAAGTATGGTGACCAAGGCTGGATGATAATGCTCACATCCTTCTTGGGTTTATCTAATGGTTACCTCACTGTCTGTGTGCTTACTTCAGCACCCAAAGGTTACAAGGGGCCAGAGCAAAATGCTTTAGGAAACTTGCTGGTTGTGTTCCTACTGGTAGGTATATTTGCAGGTGTAACACTTGATTGGTTGTGGCTTATAGGCAAGGGCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

418

Amino Acids

45.77

Weight (kDa)

8.14

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 209
AarI CACCTGC 1 cut(s) 1199
Acc36I ACCTGC 1 cut(s) 1199
AccBSI CCGCTC 1 cut(s) 754
AccII CGCG 1 cut(s) 148
AciI CCGC 5 cut(s) 146, 159, 657, 754, 947
AclWI GGATC 2 cut(s) 676, 874
AcsI RAATTY 3 cut(s) 351, 523, 956
AcuI CTGAAG 3 cut(s) 645, 741, 1110
AfaI GTAC 4 cut(s) 182, 641, 883, 914
AfiI CCNNNNNNNGG 2 cut(s) 175, 967
AgsI TTSAA 1 cut(s) 782
AjnI CCWGG 1 cut(s) 841
AleI CACNNNNGTG 1 cut(s) 176
AluBI AGCT 6 cut(s) 37, 314, 551, 586, 625, 876
AluI AGCT 6 cut(s) 37, 314, 551, 586, 625, 876
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 2 cut(s) 676, 874
AoxI GGCC 3 cut(s) 331, 654, 1148
ApeKI GCWGC 6 cut(s) 53, 368, 467, 512, 695, 775
ApoI RAATTY 3 cut(s) 351, 523, 956
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 2 cut(s) 166, 1148
AsuC2I CCSGG 1 cut(s) 1022
AsuHPI GGTGA 2 cut(s) 964, 1061
AvaII GGWCC 1 cut(s) 166
BanII GRGCYC 2 cut(s) 481, 987
BbsI GAAGAC 2 cut(s) 530, 864
BbvI GCAGC 6 cut(s) 40, 355, 454, 524, 682, 762
BccI CCATC 1 cut(s) 162
BcgI CGANNNNNNTGC 2 cut(s) 555, 589
BciT130I CCWGG 1 cut(s) 843
BcnI CCSGG 1 cut(s) 1022
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 4 cut(s) 197, 243, 299, 749
BfuAI ACCTGC 1 cut(s) 1199
BglII AGATCT 1 cut(s) 745
BisI GCNGC 8 cut(s) 54, 369, 468, 513, 657, 696, 755, 776
BlsI GCNGC 8 cut(s) 55, 370, 469, 514, 658, 697, 756, 777
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 2 cut(s) 843, 1022
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 166, 1148
BmiI GGNNCC 3 cut(s) 167, 478, 1149
BmrFI CCNGG 2 cut(s) 843, 1022
BmsI GCATC 4 cut(s) 77, 376, 479, 714
BpiI GAAGAC 2 cut(s) 530, 864
BpuEI CTTGAG 2 cut(s) 59, 947
BpuMI CCSGG 1 cut(s) 1022
BsaBI GATNNNNATC 1 cut(s) 850
BsaI GGTCTC 1 cut(s) 408
BsaJI CCNNGG 3 cut(s) 334, 841, 1054
BsaWI WCCGGW 1 cut(s) 863
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 967
Bse1I ACTGG 2 cut(s) 900, 1197
Bse3DI GCAATG 3 cut(s) 63, 713, 915
Bse8I GATNNNNATC 1 cut(s) 850
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 3 cut(s) 334, 841, 1054
BseGI GGATG 6 cut(s) 92, 154, 441, 705, 1068, 1076
BseJI GATNNNNATC 1 cut(s) 850
BseLI CCNNNNNNNGG 2 cut(s) 175, 967
BseMI GCAATG 3 cut(s) 63, 713, 915
BseNI ACTGG 2 cut(s) 900, 1197
BseRI GAGGAG 1 cut(s) 342
BseXI GCAGC 6 cut(s) 40, 355, 454, 524, 682, 762
BseYI CCCAGC 1 cut(s) 757
Bsh1236I CGCG 1 cut(s) 148
BshFI GGCC 3 cut(s) 333, 656, 1150
BsiSI CCGG 2 cut(s) 864, 1022
BslFI GGGAC 1 cut(s) 1004
BslI CCNNNNNNNGG 2 cut(s) 175, 967
BsmAI GTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 1004
BsmI GAATGC 1 cut(s) 1025
BsnI GGCC 3 cut(s) 333, 656, 1150
Bso31I GGTCTC 1 cut(s) 408
Bsp1286I GDGCHC 2 cut(s) 481, 987
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 5 cut(s) 576, 668, 745, 866, 925
BspACI CCGC 5 cut(s) 146, 159, 657, 754, 947
BspANI GGCC 3 cut(s) 333, 656, 1150
BspFNI CGCG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 3 cut(s) 167, 478, 1149
BspMI ACCTGC 1 cut(s) 1199
BspPI GGATC 2 cut(s) 676, 874
BspQI GCTCTTC 1 cut(s) 729
BspTNI GGTCTC 1 cut(s) 408
BsrBI CCGCTC 1 cut(s) 754
BsrDI GCAATG 3 cut(s) 63, 713, 915
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 2 cut(s) 900, 1197
BssECI CCNNGG 3 cut(s) 334, 841, 1054
BssMI GATC 5 cut(s) 576, 668, 745, 866, 925
BssT1I CCWWGG 2 cut(s) 334, 1054
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 107, 679, 1111
Bst6I CTCTTC 2 cut(s) 577, 729
BstAUI TGTACA 1 cut(s) 912
BstC8I GCNNGC 1 cut(s) 71
BstEII GGTNACC 2 cut(s) 1049, 1100
BstF5I GGATG 6 cut(s) 92, 154, 441, 705, 1068, 1076
BstFNI CGCG 1 cut(s) 148
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 5 cut(s) 579, 671, 748, 869, 928
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 5 cut(s) 576, 668, 745, 866, 925
BstMWI GCNNNNNNNGC 5 cut(s) 193, 365, 464, 476, 692
BstNI CCWGG 1 cut(s) 843
BstPI GGTNACC 2 cut(s) 1049, 1100
BstSCI CCNGG 2 cut(s) 841, 1020
BstUI CGCG 1 cut(s) 148
BstV1I GCAGC 6 cut(s) 40, 355, 454, 524, 682, 762
BstV2I GAAGAC 2 cut(s) 530, 864
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
BsuRI GGCC 3 cut(s) 333, 656, 1150
BtsCI GGATG 6 cut(s) 92, 154, 441, 705, 1068, 1076
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 3 cut(s) 893, 1035, 1107
BveI ACCTGC 1 cut(s) 1199
Cac8I GCNNGC 1 cut(s) 71
CciI TCATGA 1 cut(s) 223
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 2 cut(s) 166, 1148
Csp6I GTAC 4 cut(s) 181, 640, 882, 913
CviAII CATG 6 cut(s) 99, 224, 392, 427, 885, 988
CviQI GTAC 4 cut(s) 181, 640, 882, 913
DpnI GATC 5 cut(s) 578, 670, 747, 868, 927
DpnII GATC 5 cut(s) 576, 668, 745, 866, 925
Eam1104I CTCTTC 2 cut(s) 577, 729
EarI CTCTTC 2 cut(s) 577, 729
Eco130I CCWWGG 2 cut(s) 334, 1054
Eco147I AGGCCT 1 cut(s) 333
Eco24I GRGCYC 2 cut(s) 481, 987
Eco31I GGTCTC 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 166
Eco57I CTGAAG 3 cut(s) 645, 741, 1110
Eco91I GGTNACC 2 cut(s) 1049, 1100
EcoO109I RGGNCCY 1 cut(s) 166
EcoO65I GGTNACC 2 cut(s) 1049, 1100
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 2 cut(s) 334, 1054
EcoT38I GRGCYC 2 cut(s) 481, 987
ErhI CCWWGG 2 cut(s) 334, 1054
FaeI CATG 6 cut(s) 102, 227, 395, 430, 888, 991
FaqI GGGAC 1 cut(s) 1004
FatI CATG 6 cut(s) 98, 223, 391, 426, 884, 987
FauI CCCGC 2 cut(s) 153, 166
Fnu4HI GCNGC 8 cut(s) 54, 369, 468, 513, 657, 696, 755, 776
FokI GGATG 6 cut(s) 99, 141, 428, 692, 1063, 1075
FriOI GRGCYC 2 cut(s) 481, 987
Fsp4HI GCNGC 8 cut(s) 54, 369, 468, 513, 657, 696, 755, 776
FspBI CTAG 4 cut(s) 197, 243, 299, 749
GlaI GCGC 1 cut(s) 195
GluI GCNGC 8 cut(s) 54, 369, 468, 513, 657, 696, 755, 776
GsaI CCCAGC 1 cut(s) 761
HaeIII GGCC 3 cut(s) 333, 656, 1150
HapII CCGG 2 cut(s) 864, 1022
HhaI GCGC 1 cut(s) 196
Hin1II CATG 6 cut(s) 102, 227, 395, 430, 888, 991
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 3 cut(s) 598, 846, 997
HpaII CCGG 2 cut(s) 864, 1022
HphI GGTGA 2 cut(s) 964, 1061
Hpy166II GTNNAC 2 cut(s) 136, 181
Hpy188I TCNGA 6 cut(s) 543, 621, 664, 856, 925, 930
Hpy188III TCNNGA 4 cut(s) 224, 432, 474, 749
Hpy8I GTNNAC 2 cut(s) 136, 181
HpyAV CCTTC 2 cut(s) 659, 1090
HpyCH4III ACNGT 3 cut(s) 107, 679, 1111
HpyCH4V TGCA 9 cut(s) 56, 383, 397, 470, 566, 797, 893, 908, 1208
HpyF10VI GCNNNNNNNGC 5 cut(s) 193, 365, 464, 476, 692
Hsp92II CATG 6 cut(s) 102, 227, 395, 430, 888, 991
HspAI GCGC 1 cut(s) 194
Kzo9I GATC 5 cut(s) 576, 668, 745, 866, 925
LguI GCTCTTC 1 cut(s) 729
LmnI GCTCC 2 cut(s) 476, 548
Lsp1109I GCAGC 6 cut(s) 40, 355, 454, 524, 682, 762
LweI GCATC 4 cut(s) 77, 376, 479, 714
MaeI CTAG 4 cut(s) 197, 243, 299, 749
MaeIII GTNAC 4 cut(s) 1049, 1100, 1139, 1213
MalI GATC 5 cut(s) 578, 670, 747, 868, 927
MbiI CCGCTC 1 cut(s) 754
MboI GATC 5 cut(s) 576, 668, 745, 866, 925
MboII GAAGA 6 cut(s) 134, 244, 530, 564, 746, 869
MfeI CAATTG 2 cut(s) 630, 770
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 2 cut(s) 481, 987
MluCI AATT 9 cut(s) 204, 351, 378, 501, 523, 630, 770, 834, 956
MmeI TCCRAC 2 cut(s) 391, 948
MseI TTAA 2 cut(s) 500, 636
MslI CAYNNNNRTG 2 cut(s) 176, 992
MspA1I CMGCKG 1 cut(s) 949
MspI CCGG 2 cut(s) 864, 1022
MspR9I CCNGG 2 cut(s) 843, 1022
MunI CAATTG 2 cut(s) 630, 770
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 1 cut(s) 843
MvnI CGCG 1 cut(s) 148
MwoI GCNNNNNNNGC 5 cut(s) 193, 365, 464, 476, 692
NciI CCSGG 1 cut(s) 1022
NdeII GATC 5 cut(s) 576, 668, 745, 866, 925
NlaIII CATG 6 cut(s) 102, 227, 395, 430, 888, 991
NlaIV GGNNCC 3 cut(s) 167, 478, 1149
NmuCI GTSAC 1 cut(s) 1049
OliI CACNNNNGTG 1 cut(s) 176
PagI TCATGA 1 cut(s) 223
PaqCI CACCTGC 1 cut(s) 1199
PceI AGGCCT 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 729
PctI GAATGC 1 cut(s) 1025
PfeI GAWTC 3 cut(s) 598, 846, 997
PkrI GCNGC 8 cut(s) 55, 370, 469, 514, 658, 697, 756, 777
PpuMI RGGWCCY 1 cut(s) 166
PsiI TTATAA 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 841
PspEI GGTNACC 2 cut(s) 1049, 1100
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 3 cut(s) 167, 478, 1149
PspPI GGNCC 2 cut(s) 166, 1148
PspPPI RGGWCCY 1 cut(s) 166
PsuI RGATCY 1 cut(s) 745
RsaI GTAC 4 cut(s) 182, 641, 883, 914
RsaNI GTAC 4 cut(s) 181, 640, 882, 913
RseI CAYNNNNRTG 2 cut(s) 176, 992
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 2 cut(s) 500, 636
SatI GCNGC 8 cut(s) 54, 369, 468, 513, 657, 696, 755, 776
Sau3AI GATC 5 cut(s) 576, 668, 745, 866, 925
Sau96I GGNCC 2 cut(s) 166, 1148
ScaI AGTACT 1 cut(s) 641
ScrFI CCNGG 2 cut(s) 843, 1022
SduI GDGCHC 2 cut(s) 481, 987
SfaNI GCATC 4 cut(s) 77, 376, 479, 714
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 2 cut(s) 176, 992
SmlI CTYRAG 2 cut(s) 38, 962
SmoI CTYRAG 2 cut(s) 38, 962
Sse9I AATT 9 cut(s) 204, 351, 378, 501, 523, 630, 770, 834, 956
SseBI AGGCCT 1 cut(s) 333
SsiI CCGC 5 cut(s) 146, 159, 657, 754, 947
SspMI CTAG 4 cut(s) 197, 243, 299, 749
StuI AGGCCT 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 841, 1020
StyI CCWWGG 2 cut(s) 334, 1054
TaaI ACNGT 3 cut(s) 107, 679, 1111
TaqI TCGA 2 cut(s) 575, 801
TasI AATT 9 cut(s) 204, 351, 378, 501, 523, 630, 770, 834, 956
TatI WGTACW 2 cut(s) 639, 912
TauI GCSGC 2 cut(s) 659, 757
TfiI GAWTC 3 cut(s) 598, 846, 997
Tru1I TTAA 2 cut(s) 500, 636
Tru9I TTAA 2 cut(s) 500, 636
TscAI CASTG 3 cut(s) 900, 1042, 1114
TseFI GTSAC 1 cut(s) 1049
TseI GCWGC 6 cut(s) 53, 368, 467, 512, 695, 775
Tsp45I GTSAC 1 cut(s) 1049
TspDTI ATGAA 4 cut(s) 87, 251, 268, 415
TspRI CASTG 3 cut(s) 900, 1042, 1114
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 3 cut(s) 351, 523, 956
XbaI TCTAGA 1 cut(s) 748
XspI CTAG 4 cut(s) 197, 243, 299, 749
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.