Rorug02G0488300

Equilibrative nucleotide transporter 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008216
Physical Location & Seq
Forward (+)
61818705 .. 61819234
530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0488300.1

Sequence Viewer

Length: 456 bp
ATGGCTCCAGGACCTATCCAAAAGTTTATGGTTACTGAAAATGAAATATTTATTCATAAGATTGTATGGACACGTCCATTCGCAGGATCTGAAAATGCAGCGTTTAAAGCTCTCTTGGCAGGAGCGGCAGGTCAGGACGCAAGGTCAACTTCAGACGCTGCATTGATGGCCTTGACTGCTGCTGTCGGCTTAGCAGTAAGTCAAGGTGGAGACTTTCTCGAAATAGAGGGAGAGAATGAAACTACGTTCTCCTTGGTCACAGGCCAGATCCCTCCATCAGATGAACTGACAAGGGAGCTTATGTTTAGGTGCCTAAGGGAGCTGCAGAATTTCAGAAGGGTGGAGTTTCGCTATGTGAAACCTGACACCAATGAGGCGGCCAACCAGCTAGCCAGAATGGCCATGGAGACGGAGGAGAGCTGGTTTTTTATGCAGACAACCCTCCACCTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

151

Amino Acids

16.84

Weight (kDa)

4.95

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 40 - 134 2.9e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 309
AccBSI CCGCTC 1 cut(s) 125
AciI CCGC 2 cut(s) 125, 377
AclWI GGATC 2 cut(s) 94, 262
AcoI YGGCCR 2 cut(s) 378, 399
AcsI RAATTY 1 cut(s) 328
AcuI CTGAAG 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 71
AhdI GACNNNNNGTC 1 cut(s) 142
AjiI CACGTC 1 cut(s) 74
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 5 cut(s) 110, 298, 322, 388, 420
AluI AGCT 5 cut(s) 110, 298, 322, 388, 420
Alw26I GTCTC 2 cut(s) 204, 401
AlwI GGATC 2 cut(s) 94, 262
AlwNI CAGNNNCTG 2 cut(s) 89, 158
AoxI GGCC 4 cut(s) 168, 262, 378, 399
ApeKI GCWGC 4 cut(s) 98, 158, 179, 322
ApoI RAATTY 1 cut(s) 328
AspS9I GGNCC 1 cut(s) 11
AsuNHI GCTAGC 1 cut(s) 388
AvaII GGWCC 1 cut(s) 11
AxyI CCTNAGG 1 cut(s) 314
BalI TGGCCA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 309
BbvI GCAGC 4 cut(s) 110, 145, 166, 309
BccI CCATC 2 cut(s) 160, 283
BciT130I CCWGG 1 cut(s) 9
BcoDI GTCTC 2 cut(s) 204, 401
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 323
BfuAI ACCTGC 1 cut(s) 119
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 6 cut(s) 99, 126, 159, 180, 323, 378
BlpI GCTNAGC 1 cut(s) 190
BlsI GCNGC 6 cut(s) 100, 127, 160, 181, 324, 379
Bme1390I CCNGG 1 cut(s) 9
Bme18I GGWCC 1 cut(s) 11
BmeRI GACNNNNNGTC 1 cut(s) 142
BmgBI CACGTC 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 2 cut(s) 6, 311
BmrFI CCNGG 1 cut(s) 9
BmtI GCTAGC 1 cut(s) 392
BplI GAGNNNNNCTC 2 cut(s) 201, 233
Bpu1102I GCTNAGC 1 cut(s) 190
BsaJI CCNNGG 2 cut(s) 252, 402
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 407, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse21I CCTNAGG 1 cut(s) 314
BseBI CCWGG 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 252, 402
BseLI CCNNNNNNNGG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 4 cut(s) 110, 145, 166, 309
BshFI GGCC 4 cut(s) 170, 264, 380, 401
BshNI GGYRCC 1 cut(s) 309
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 204, 401
BsmBI CGTCTC 1 cut(s) 401
BsnI GGCC 4 cut(s) 170, 264, 380, 401
Bsp143I GATC 2 cut(s) 86, 267
Bsp1720I GCTNAGC 1 cut(s) 190
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 2 cut(s) 125, 377
BspANI GGCC 4 cut(s) 170, 264, 380, 401
BspLI GGNNCC 2 cut(s) 6, 311
BspMAI CTGCAG 1 cut(s) 327
BspMI ACCTGC 1 cut(s) 119
BspOI GCTAGC 1 cut(s) 392
BspPI GGATC 2 cut(s) 94, 262
BspT107I GGYRCC 1 cut(s) 309
BsrBI CCGCTC 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 252, 402
BssMI GATC 2 cut(s) 86, 267
BssT1I CCWWGG 2 cut(s) 252, 402
Bst2UI CCWGG 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 2 cut(s) 190, 314
BstDSI CCRYGG 1 cut(s) 402
BstKTI GATC 2 cut(s) 89, 270
BstMAI GTCTC 2 cut(s) 204, 401
BstMBI GATC 2 cut(s) 86, 267
BstMWI GCNNNNNNNGC 6 cut(s) 107, 116, 125, 167, 176, 398
BstNI CCWGG 1 cut(s) 9
BstSCI CCNGG 1 cut(s) 7
BstSFI CTRYAG 1 cut(s) 323
BstV1I GCAGC 4 cut(s) 110, 145, 166, 309
BstX2I RGATCY 2 cut(s) 86, 267
BstYI RGATCY 2 cut(s) 86, 267
Bsu36I CCTNAGG 1 cut(s) 314
BsuRI GGCC 4 cut(s) 170, 264, 380, 401
BtgI CCRYGG 1 cut(s) 402
BtrI CACGTC 1 cut(s) 74
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 390
CaiI CAGNNNCTG 2 cut(s) 89, 158
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 2 cut(s) 146, 164
CviAII CATG 1 cut(s) 403
DdeI CTNAG 2 cut(s) 190, 314
DpnI GATC 2 cut(s) 88, 269
DpnII GATC 2 cut(s) 86, 267
DraI TTTAAA 1 cut(s) 106
DriI GACNNNNNGTC 1 cut(s) 142
EaeI YGGCCR 2 cut(s) 378, 399
Eam1105I GACNNNNNGTC 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 252, 402
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 135
Eco81I CCTNAGG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 7
EcoT14I CCWWGG 2 cut(s) 252, 402
ErhI CCWWGG 2 cut(s) 252, 402
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 1 cut(s) 406
FaiI YATR 7 cut(s) 29, 57, 67, 302, 354, 404, 431
FalI AAGNNNNNCTT 2 cut(s) 133, 165
FatI CATG 1 cut(s) 402
Fnu4HI GCNGC 6 cut(s) 99, 126, 159, 180, 323, 378
Fsp4HI GCNGC 6 cut(s) 99, 126, 159, 180, 323, 378
FspBI CTAG 1 cut(s) 389
GluI GCNGC 6 cut(s) 99, 126, 159, 180, 323, 378
HaeIII GGCC 4 cut(s) 170, 264, 380, 401
HgaI GACGC 2 cut(s) 146, 164
Hin1II CATG 1 cut(s) 406
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 4 cut(s) 91, 154, 280, 335
Hpy188III TCNNGA 2 cut(s) 134, 218
Hpy8I GTNNAC 1 cut(s) 147
HpyAV CCTTC 1 cut(s) 330
HpyCH4IV ACGT 2 cut(s) 73, 245
HpyCH4V TGCA 4 cut(s) 98, 161, 325, 433
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 116, 125, 167, 176, 398
HpyF3I CTNAG 2 cut(s) 190, 314
HpySE526I ACGT 2 cut(s) 73, 245
Hsp92II CATG 1 cut(s) 406
Kzo9I GATC 2 cut(s) 86, 267
LmnI GCTCC 4 cut(s) 10, 122, 295, 319
Lsp1109I GCAGC 4 cut(s) 110, 145, 166, 309
MaeI CTAG 1 cut(s) 389
MaeII ACGT 2 cut(s) 73, 245
MaeIII GTNAC 2 cut(s) 31, 256
MalI GATC 2 cut(s) 88, 269
MbiI CCGCTC 1 cut(s) 125
MboI GATC 2 cut(s) 86, 267
MflI RGATCY 2 cut(s) 86, 267
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 1 cut(s) 328
MluNI TGGCCA 1 cut(s) 401
MnlI CCTC 5 cut(s) 220, 282, 367, 406, 452
Mox20I TGGCCA 1 cut(s) 401
MscI TGGCCA 1 cut(s) 401
MseI TTAA 1 cut(s) 105
Msp20I TGGCCA 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 6 cut(s) 107, 116, 125, 167, 176, 398
NcoI CCATGG 1 cut(s) 402
NdeII GATC 2 cut(s) 86, 267
NheI GCTAGC 1 cut(s) 388
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 2 cut(s) 6, 311
NmuCI GTSAC 1 cut(s) 256
PfoI TCCNGGA 1 cut(s) 7
PkrI GCNGC 6 cut(s) 100, 127, 160, 181, 324, 379
PpuMI RGGWCCY 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 2 cut(s) 6, 311
PspPI GGNCC 1 cut(s) 11
PspPPI RGGWCCY 1 cut(s) 11
PstI CTGCAG 1 cut(s) 327
PstNI CAGNNNCTG 2 cut(s) 89, 158
PsuI RGATCY 2 cut(s) 86, 267
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 6 cut(s) 99, 126, 159, 180, 323, 378
Sau3AI GATC 2 cut(s) 86, 267
Sau96I GGNCC 1 cut(s) 11
ScrFI CCNGG 1 cut(s) 9
SfcI CTRYAG 1 cut(s) 323
SinI GGWCC 1 cut(s) 11
Sse9I AATT 1 cut(s) 328
SsiI CCGC 2 cut(s) 125, 377
SspI AATATT 1 cut(s) 48
SspMI CTAG 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 7
StyI CCWWGG 2 cut(s) 252, 402
TaiI ACGT 2 cut(s) 76, 248
TaqI TCGA 1 cut(s) 219
TasI AATT 1 cut(s) 328
TauI GCSGC 2 cut(s) 128, 380
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 4 cut(s) 98, 158, 179, 322
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 4 cut(s) 44, 57, 252, 297
TspGWI ACGGA 1 cut(s) 425
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 400
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.