MD17G1086200.v1.1

Equilibrative nucleotide transporter 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008216
Physical Location & Seq
Reverse (-)
7071777 .. 7075619
3843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1086200.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGGCAAGCATTGATGTGAATGCACCCCCTCCACAGCTTGAGGGGAAGTATGCTGCAATGTTAGTGTGTTGGCTTCTTGGAAATGGATGCCTCTTTTCATGGAACAGCATGCTCACAATTCAAGATTACTATGTTAACTTGTTCCCGCGGTACCATCCCTCGAGGGTACTTACACTGGTATACCAGCCATTCGCACTTTTAACTATCTTTGCACTGTCATACCATGAGGCGAAGATCAATACTAGGAAGCGAAACCTATTAGGATACATTCTGTTTTTCCTAAGTTCTTTGTTGGTGCTCGTTTTGGATTTAGCTACATCCGGGAAAGGAGGTTTAGGAACTTTTATCGGAATTTGTGCAATTAGTGCTGCCTTCGGAATTGCAGATGCTCATGTGCAAGGTGGAATGGTTGGAGACCTCTCTTTCATGCCAGTTGAGCTCATCCAATCTTTCCTTGCTGGTTCGGCTGCATCAGGAGCTCTAACCTCTGCTTTGAGGCTGATTACAAAGGCGGCATTTGAGAATTCCCAAAATGGTCTTCGAAAGGGAGCCATTTTGTTCTTCGCCATCTCTTCATTCTTCGAGCTTCTCTGCGTTTTCCTCTATGCTTTTTCCTTTCCAAAGCTGCCAATTGTTAAGTACTATCGCTCAAAGGCAGCTTCCGAAGGATCTAAAACTGTTTCAGCTGACCTTGCTGCCGGTGGCATCCAAAAATTGCCAGAATCAACAGATGAGGAAAATCGAAAAGAACCTGACCGACTTGGGAACAAACAGCTGTTGCTTCAAAACATTGATTATGCACTTGGCATGTTTCTAATCTATACACTCACTCTGTCAATTTTCCCCGGATTCTTATCAGAAGACACTGGATCTCACAGTTTGGGAACATGGTATGCACTCGTTTTGATTACAATGTACAATGTGTGTGATCTCATCGGAAGATACATTCCGCTAGTGAAATTCCTGAAGATGGAATCGCGCAGAGGGCTCATGATTGTGATTCTTTCACGCTTCTTACTTGTCCCGTGTTTCTATTTCACGGCCAAGTATGGTGACCAAGGCTGGATGATCATGCTCACATCCTTCTTAGGTTTAACCAACGGTTACCTCACTGTCTGCGTCCTCACTTCAGCACCTAAGGGCTACAAGGGGCCGGAGCAAAATGCTTTGGGAAATCTTCTGGTGATGTTCTTGCTTGGAGGTATATTTGCAGGGGTGACACTTGACTGGTTGTGGCTTATTGGGAAGGGCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

419

Amino Acids

45.98

Weight (kDa)

8.81

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 126 - 412 4.8e-38 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 160
Acc65I GGTACC 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 180
AccII CGCG 2 cut(s) 148, 979
AciI CCGC 4 cut(s) 146, 148, 512, 950
AclWI GGATC 2 cut(s) 676, 877
AcoI YGGCCR 1 cut(s) 1041
AcsI RAATTY 3 cut(s) 351, 523, 959
AcuI CTGAAG 2 cut(s) 986, 1113
AfaI GTAC 4 cut(s) 152, 168, 641, 917
AfiI CCNNNNNNNGG 1 cut(s) 970
AgsI TTSAA 2 cut(s) 122, 785
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
AluBI AGCT 9 cut(s) 37, 314, 439, 479, 586, 625, 659, 686, 775
AluI AGCT 9 cut(s) 37, 314, 439, 479, 586, 625, 659, 686, 775
Alw21I GWGCWC 3 cut(s) 300, 441, 481
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 2 cut(s) 676, 877
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 2 cut(s) 1041, 1151
ApeKI GCWGC 6 cut(s) 53, 368, 467, 625, 656, 695
ApoI RAATTY 3 cut(s) 351, 523, 959
Asp718I GGTACC 1 cut(s) 150
AspLEI GCGC 1 cut(s) 981
AspS9I GGNCC 1 cut(s) 1151
AsuC2I CCSGG 2 cut(s) 322, 846
AsuHPI GGTGA 3 cut(s) 1064, 1195, 1228
AsuII TTCGAA 1 cut(s) 541
AvaI CYCGRG 1 cut(s) 160
AxyI CCTNAGG 1 cut(s) 1137
BanI GGYRCC 1 cut(s) 150
BanII GRGCYC 3 cut(s) 441, 481, 990
BbsI GAAGAC 2 cut(s) 530, 867
Bbv12I GWGCWC 3 cut(s) 300, 441, 481
BbvI GCAGC 6 cut(s) 40, 355, 454, 612, 668, 682
BccI CCATC 3 cut(s) 162, 575, 964
BceAI ACGGC 1 cut(s) 1056
BciVI GTATCC 1 cut(s) 257
BclI TGATCA 1 cut(s) 1068
BcnI CCSGG 2 cut(s) 322, 846
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 2 cut(s) 243, 953
BfuI GTATCC 1 cut(s) 257
BisI GCNGC 7 cut(s) 54, 369, 468, 513, 626, 657, 696
BlsI GCNGC 7 cut(s) 55, 370, 469, 514, 627, 658, 697
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 2 cut(s) 322, 846
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 1151
BmiI GGNNCC 3 cut(s) 152, 550, 1152
BmrFI CCNGG 2 cut(s) 322, 846
BmsI GCATC 4 cut(s) 77, 376, 479, 714
BpiI GAAGAC 2 cut(s) 530, 867
Bpu14I TTCGAA 1 cut(s) 541
BpuEI CTTGAG 1 cut(s) 59
BpuMI CCSGG 2 cut(s) 322, 846
BsaBI GATNNNNATC 1 cut(s) 853
BsaI GGTCTC 1 cut(s) 408
BsaJI CCNNGG 3 cut(s) 146, 844, 1057
Bsc4I CCNNNNNNNGG 1 cut(s) 970
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 4 cut(s) 180, 431, 871, 1232
Bse21I CCTNAGG 1 cut(s) 1137
Bse3DI GCAATG 1 cut(s) 63
Bse8I GATNNNNATC 1 cut(s) 853
BseDI CCNNGG 3 cut(s) 146, 844, 1057
BseGI GGATG 7 cut(s) 92, 154, 317, 441, 705, 1071, 1079
BseJI GATNNNNATC 1 cut(s) 853
BseLI CCNNNNNNNGG 1 cut(s) 970
BseMI GCAATG 1 cut(s) 63
BseNI ACTGG 4 cut(s) 180, 431, 871, 1232
BseXI GCAGC 6 cut(s) 40, 355, 454, 612, 668, 682
Bsh1236I CGCG 2 cut(s) 148, 979
BshFI GGCC 2 cut(s) 1043, 1153
BshNI GGYRCC 1 cut(s) 150
BsiHKAI GWGCWC 3 cut(s) 300, 441, 481
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 4 cut(s) 321, 699, 846, 1154
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 1 cut(s) 970
BsmAI GTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 1007
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 2 cut(s) 1043, 1153
Bso31I GGTCTC 1 cut(s) 408
BsoBI CYCGRG 1 cut(s) 160
Bsp119I TTCGAA 1 cut(s) 541
Bsp1286I GDGCHC 4 cut(s) 300, 441, 481, 990
Bsp1407I TGTACA 1 cut(s) 915
Bsp143I GATC 5 cut(s) 234, 668, 869, 928, 1068
BspACI CCGC 4 cut(s) 146, 148, 512, 950
BspANI GGCC 2 cut(s) 1043, 1153
BspFNI CGCG 2 cut(s) 148, 979
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 3 cut(s) 152, 550, 1152
BspPI GGATC 2 cut(s) 676, 877
BspT104I TTCGAA 1 cut(s) 541
BspT107I GGYRCC 1 cut(s) 150
BspTNI GGTCTC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 698
BsrGI TGTACA 1 cut(s) 915
BsrI ACTGG 4 cut(s) 180, 431, 871, 1232
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 3 cut(s) 146, 844, 1057
BssMI GATC 5 cut(s) 234, 668, 869, 928, 1068
BssNAI GTATAC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 1057
Bst1107I GTATAC 1 cut(s) 181
Bst4CI ACNGT 5 cut(s) 216, 679, 878, 1103, 1114
Bst6I CTCTTC 1 cut(s) 577
BstAPI GCANNNNNTGC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 915
BstBI TTCGAA 1 cut(s) 541
BstC8I GCNNGC 2 cut(s) 7, 110
BstDEI CTNAG 3 cut(s) 281, 1087, 1137
BstDSI CCRYGG 1 cut(s) 146
BstEII GGTNACC 2 cut(s) 1052, 1103
BstF5I GGATG 7 cut(s) 92, 154, 317, 441, 705, 1071, 1079
BstFNI CGCG 2 cut(s) 148, 979
BstHHI GCGC 1 cut(s) 981
BstKTI GATC 5 cut(s) 237, 671, 872, 931, 1071
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 5 cut(s) 234, 668, 869, 928, 1068
BstMWI GCNNNNNNNGC 6 cut(s) 365, 436, 464, 476, 692, 985
BstNSI RCATGY 2 cut(s) 112, 811
BstPI GGTNACC 2 cut(s) 1052, 1103
BstSCI CCNGG 2 cut(s) 320, 844
BstUI CGCG 2 cut(s) 148, 979
BstV1I GCAGC 6 cut(s) 40, 355, 454, 612, 668, 682
BstV2I GAAGAC 2 cut(s) 530, 867
BstX2I RGATCY 2 cut(s) 668, 869
BstYI RGATCY 2 cut(s) 668, 869
BstZ17I GTATAC 1 cut(s) 181
Bsu36I CCTNAGG 1 cut(s) 1137
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 2 cut(s) 1043, 1153
BtgI CCRYGG 1 cut(s) 146
BtsCI GGATG 7 cut(s) 92, 154, 317, 441, 705, 1071, 1079
BtsIMutI CAGTG 4 cut(s) 173, 212, 864, 1110
Cac8I GCNNGC 2 cut(s) 7, 110
CciI TCATGA 1 cut(s) 990
CfoI GCGC 1 cut(s) 981
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 1 cut(s) 1151
Cfr42I CCGCGG 1 cut(s) 149
CseI GACGC 1 cut(s) 1108
Csp6I GTAC 4 cut(s) 151, 167, 640, 916
CviAII CATG 9 cut(s) 99, 109, 224, 392, 427, 808, 888, 991, 1072
CviQI GTAC 4 cut(s) 151, 167, 640, 916
DdeI CTNAG 3 cut(s) 281, 1087, 1137
DpnI GATC 5 cut(s) 236, 670, 871, 930, 1070
DpnII GATC 5 cut(s) 234, 668, 869, 928, 1068
EaeI YGGCCR 1 cut(s) 1041
Eam1104I CTCTTC 1 cut(s) 577
EarI CTCTTC 1 cut(s) 577
Ecl136II GAGCTC 2 cut(s) 439, 479
Eco130I CCWWGG 1 cut(s) 1057
Eco24I GRGCYC 3 cut(s) 441, 481, 990
Eco31I GGTCTC 1 cut(s) 408
Eco53kI GAGCTC 2 cut(s) 439, 479
Eco57I CTGAAG 2 cut(s) 986, 1113
Eco81I CCTNAGG 1 cut(s) 1137
Eco88I CYCGRG 1 cut(s) 160
Eco91I GGTNACC 2 cut(s) 1052, 1103
EcoICRI GAGCTC 2 cut(s) 439, 479
EcoO65I GGTNACC 2 cut(s) 1052, 1103
EcoRI GAATTC 1 cut(s) 523
EcoT14I CCWWGG 1 cut(s) 1057
EcoT38I GRGCYC 3 cut(s) 441, 481, 990
ErhI CCWWGG 1 cut(s) 1057
FaeI CATG 9 cut(s) 102, 112, 227, 395, 430, 811, 891, 994, 1075
FaqI GGGAC 1 cut(s) 1007
FatI CATG 9 cut(s) 98, 108, 223, 391, 426, 807, 887, 990, 1071
FauI CCCGC 1 cut(s) 153
FbaI TGATCA 1 cut(s) 1068
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 7 cut(s) 54, 369, 468, 513, 626, 657, 696
FokI GGATG 7 cut(s) 99, 141, 304, 428, 692, 1066, 1078
FriOI GRGCYC 3 cut(s) 441, 481, 990
Fsp4HI GCNGC 7 cut(s) 54, 369, 468, 513, 626, 657, 696
FspBI CTAG 2 cut(s) 243, 953
GlaI GCGC 1 cut(s) 980
GluI GCNGC 7 cut(s) 54, 369, 468, 513, 626, 657, 696
HaeIII GGCC 2 cut(s) 1043, 1153
HapII CCGG 4 cut(s) 321, 699, 846, 1154
HgaI GACGC 1 cut(s) 1108
HhaI GCGC 1 cut(s) 981
Hin1II CATG 9 cut(s) 102, 112, 227, 395, 430, 811, 891, 994, 1075
Hin6I GCGC 1 cut(s) 979
HinP1I GCGC 1 cut(s) 979
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 4 cut(s) 722, 849, 974, 1000
HpaI GTTAAC 1 cut(s) 136
HpaII CCGG 4 cut(s) 321, 699, 846, 1154
HphI GGTGA 3 cut(s) 1064, 1195, 1228
Hpy166II GTNNAC 2 cut(s) 136, 181
Hpy188I TCNGA 5 cut(s) 350, 377, 664, 859, 938
Hpy188III TCNNGA 4 cut(s) 122, 474, 964, 991
Hpy8I GTNNAC 2 cut(s) 136, 181
HpyAV CCTTC 4 cut(s) 382, 659, 1093, 1240
HpyCH4III ACNGT 5 cut(s) 216, 679, 878, 1103, 1114
HpyF10VI GCNNNNNNNGC 6 cut(s) 365, 436, 464, 476, 692, 985
HpyF3I CTNAG 3 cut(s) 281, 1087, 1137
Hsp92II CATG 9 cut(s) 102, 112, 227, 395, 430, 811, 891, 994, 1075
HspAI GCGC 1 cut(s) 979
KpnI GGTACC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 1068
KspAI GTTAAC 1 cut(s) 136
KspI CCGCGG 1 cut(s) 149
Kzo9I GATC 5 cut(s) 234, 668, 869, 928, 1068
LmnI GCTCC 3 cut(s) 476, 548, 1156
Lsp1109I GCAGC 6 cut(s) 40, 355, 454, 612, 668, 682
LweI GCATC 4 cut(s) 77, 376, 479, 714
MaeI CTAG 2 cut(s) 243, 953
MaeIII GTNAC 3 cut(s) 1052, 1103, 1216
MalI GATC 5 cut(s) 236, 670, 871, 930, 1070
MboI GATC 5 cut(s) 234, 668, 869, 928, 1068
MboII GAAGA 9 cut(s) 244, 530, 553, 564, 571, 872, 951, 979, 1169
MfeI CAATTG 1 cut(s) 630
MflI RGATCY 2 cut(s) 668, 869
MhlI GDGCHC 4 cut(s) 300, 441, 481, 990
MluCI AATT 9 cut(s) 117, 351, 360, 378, 523, 630, 713, 837, 959
MmeI TCCRAC 1 cut(s) 391
MseI TTAA 4 cut(s) 135, 200, 636, 1094
MslI CAYNNNNRTG 2 cut(s) 14, 995
MspA1I CMGCKG 3 cut(s) 148, 686, 775
MspI CCGG 4 cut(s) 321, 699, 846, 1154
MspR9I CCNGG 2 cut(s) 322, 846
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 1 cut(s) 25
MvnI CGCG 2 cut(s) 148, 979
MwoI GCNNNNNNNGC 6 cut(s) 365, 436, 464, 476, 692, 985
NciI CCSGG 2 cut(s) 322, 846
NdeII GATC 5 cut(s) 234, 668, 869, 928, 1068
NlaIII CATG 9 cut(s) 102, 112, 227, 395, 430, 811, 891, 994, 1075
NlaIV GGNNCC 3 cut(s) 152, 550, 1152
NmuCI GTSAC 2 cut(s) 1052, 1216
NspI RCATGY 2 cut(s) 112, 811
NspV TTCGAA 1 cut(s) 541
PaeI GCATGC 1 cut(s) 112
PaeR7I CTCGAG 1 cut(s) 160
PagI TCATGA 1 cut(s) 990
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 4 cut(s) 722, 849, 974, 1000
PfoI TCCNGGA 1 cut(s) 320
PkrI GCNGC 7 cut(s) 55, 370, 469, 514, 627, 658, 697
Psp124BI GAGCTC 2 cut(s) 441, 481
PspEI GGTNACC 2 cut(s) 1052, 1103
PspN4I GGNNCC 3 cut(s) 152, 550, 1152
PspPI GGNCC 1 cut(s) 1151
PspXI VCTCGAGB 1 cut(s) 160
PsuI RGATCY 2 cut(s) 668, 869
PvuII CAGCTG 2 cut(s) 686, 775
RsaI GTAC 4 cut(s) 152, 168, 641, 917
RsaNI GTAC 4 cut(s) 151, 167, 640, 916
RseI CAYNNNNRTG 2 cut(s) 14, 995
SacI GAGCTC 2 cut(s) 441, 481
SacII CCGCGG 1 cut(s) 149
SaqAI TTAA 4 cut(s) 135, 200, 636, 1094
SatI GCNGC 7 cut(s) 54, 369, 468, 513, 626, 657, 696
Sau3AI GATC 5 cut(s) 234, 668, 869, 928, 1068
Sau96I GGNCC 1 cut(s) 1151
ScaI AGTACT 1 cut(s) 641
ScrFI CCNGG 2 cut(s) 322, 846
SduI GDGCHC 4 cut(s) 300, 441, 481, 990
SfaNI GCATC 4 cut(s) 77, 376, 479, 714
Sfr274I CTCGAG 1 cut(s) 160
Sfr303I CCGCGG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 541
SgrBI CCGCGG 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 160
SmiMI CAYNNNNRTG 2 cut(s) 14, 995
SmlI CTYRAG 2 cut(s) 38, 160
SmoI CTYRAG 2 cut(s) 38, 160
SphI GCATGC 1 cut(s) 112
Sse9I AATT 9 cut(s) 117, 351, 360, 378, 523, 630, 713, 837, 959
SsiI CCGC 4 cut(s) 146, 148, 512, 950
SspMI CTAG 2 cut(s) 243, 953
SstI GAGCTC 2 cut(s) 441, 481
StyD4I CCNGG 2 cut(s) 320, 844
StyI CCWWGG 1 cut(s) 1057
TaaI ACNGT 5 cut(s) 216, 679, 878, 1103, 1114
TaqI TCGA 4 cut(s) 161, 541, 582, 742
TaqII GACCGA 1 cut(s) 771
TasI AATT 9 cut(s) 117, 351, 360, 378, 523, 630, 713, 837, 959
TatI WGTACW 2 cut(s) 639, 915
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 4 cut(s) 722, 849, 974, 1000
Tru1I TTAA 4 cut(s) 135, 200, 636, 1094
Tru9I TTAA 4 cut(s) 135, 200, 636, 1094
TscAI CASTG 4 cut(s) 180, 219, 871, 1117
TseFI GTSAC 2 cut(s) 1052, 1216
TseI GCWGC 6 cut(s) 53, 368, 467, 625, 656, 695
Tsp45I GTSAC 2 cut(s) 1052, 1216
TspDTI ATGAA 3 cut(s) 87, 415, 564
TspRI CASTG 4 cut(s) 180, 219, 871, 1117
XapI RAATTY 3 cut(s) 351, 523, 959
XceI RCATGY 2 cut(s) 112, 811
XhoI CTCGAG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 2 cut(s) 243, 953
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.