FvH4_6g49360

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002286
Physical Location & Seq
Reverse (-)
36998177 .. 37003616
5440 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49360.t1

Sequence Viewer

Length: 2871 bp
ATGGATGAGAAAGCCGAAGGGTTAGAGGTGGTGGTGAAGGAGGCTGTGGATTTGGAGCACGTACCTATTGAAGAGGTATTCCTAACCTTGAGGTGTGACGAACATGGACTCACATCTGAGGCTGCTCAGCAGAGGCTGGCCCTTTTTGGGTACAACAAGCTTGAGGAGAAGAAGGAAAGTAAGGTTTTGAAGTTCTTAGGGTTTATGTGGAACCCTCTCTCATGGGTGATGGAAGCTGCAGCTATCATGGCCATTGCTCTAGCTAATGGAGGAGGAAAGCCTCCAGATTGGCAAGATTTTGTAGGGATCATTACTCTGCTTGTCATCAATTCAACTATAAGTTTTATTGAAGAGAACAACGCTGGAAATGCTGCTGCTGCTCTCATGGCTCGTCTCGCTCCCACAGCCAAGGTTCTTCGAGATGGGAAGTGGATCGAGGAGGATGCTTCCATTCTTGTTCCTGGTGATATAATCAGCATTAAACTTGGAGACATTCTTCCAGCAGATGCCCGTCTCCTCGATGGTGATCCATTGAAAATTGATCAGTCATCACTTACAGGTGAGTCACTTCCTGTGACCAAAAGCCCTGGTGACAGTGTGTATTCTGGTTCTACATGCAAACAAGGAGAGATTGAAGCGGTGGTCATTGCCACTGGTGTTCATACCTTTTTCGGCAAGGCTGCGCACCTTGTCGATAGCACGAATCAACAGGGTCATTTTCAACAGGTCTTGACTGCAATTGGGAATTTCTGTATATGTTCCATCGCTGTGGGAATGATCATAGAGATCATAGTCATGTACCCAATACAACATCGTAGTTACCGCCCTGGTATTGACAACCTGCTTGTGCTACTTATTGGAGGAATTCCCATTGCCATGCCCACAGTTTTATCTGTGACAATGGCTATTGGTTCCCACCGGTTGGCGGAGCAGGGTGCTATCACTAAACGGATGACGGCAATTGAAGAAATGGCAGGCATGGATGTTCTATGCAGTGACAAAACTGGGACTCTGACATTGAATAAGCTTACAGTTGACAAGACTCTTTTAGAGGTCTTTGTTAAAGGGGTGGACGTAGATACTGTTGTTCTAATGGCAGCTCGAGCATCCCGGATTGAAAATCAAGATGCAATAGATGCTGCTATTGTGGGAATGTTGGCCGATCCAAAGGAGGCGAGAGCCAACATTCGAGAGGTGCACTTCCTTCCGTTCAACCCAACTGACAAGCGAACAGCTCTTACATATATTGACCTTGAAGGTAAAATGCATCGTGTCAGCAAAGGTGCACCAGAACAGATTTTGAATCTTGTGCACAATAAATCAGAGTTAGAACGCAAAGTTCATGTTATGATTGATAAGTACGCAGAGCGAGGATTAAGATCCCTAGCAGTAGCATACCAGGAAGTTCCAGAGGGAAGGAAGGAGAGCCTTGGAGGGCCTTGGCAATTCATTGCGCTGGTGCCTTTGTTTGATCCTCCTCGACATGACAGTGCAGAGACGATTCAGAGGGCATTGAATCTTGGTGTGAATGTTAAGATGATTACAGGTGATCAGCTTGCAATAGCAAAGGAGACAGGACGTCGTCTCGGTATGGGAACAAACATGTACCCTTCATCAGCTCTTTTGGGACAGAAAAAGGACGAATCAGTTGCGGGTTTGCCAGTTGATGAGCTTATTGAAAAAGCTGATGGGTTTGCTGGAGTTTTCCCTGAGCACAAGTATGAGATTGTGAAGCGTTTGCAAGCAAAAGGACATATATGTGGGATGACTGGTGATGGAGTTAATGATGCTCCTGCTCTTAAAAAAGCTGATATTGGCATAGCTGTTGCTGATGCAACTGATGCAGCTCGAAGTGCTTCTGACATTGTACTCACAGAACCTGGCCTTAGTGTCATCATAAGCGCTGTACTAACTAGTCGAGCAATATTCCAGAGAATGAAAAATTACACGATATATGCTGTTTCCATTACAATCCGTATTGTGCTTGGTTTCATGCTACTGGCACTCATATGGCACTTCGACTTTCCACCCTTCATGGTTCTGATCATTGCTATTCTCAATGATGGTACTATAATGACAATTTCAAAGGATAGAGTGAAACCCTCTCCTATGCCAGACAGTTGGAAACTAGCGGAGATCTTTGCCACCGGTATCATCCTGGGTACTTACTTAGCAGTTATGACGGTCATTTTCTTTTGGGCAGCATACGATACAGACTTCTTCCCTCGTCACTTTGGGGTTCCAAGCCTTCATAGAAAGGATAATGACAGCTATAGGAAGCTTGCTGCTGCAGTGTACCTGCAAGTGAGTACCATCAGTCAGGCTCTCATATTCGTCACCCGGGCACGTAGTTGGTCTATTCTCGAGCGACCTGGTCTTTTACTTGTAGCAGCTTTTGTTATTGCTCAGCTGATTGCTACACTCATTGCTGTCTATGCCGATTGGAGTTTCGCGGCGATAGAGGGAATTGGGTGGGGTTGGGCTGGTGTGGTTTGGTTATATAATCTCGTCTTCTATCTTCCACTTGATATCCTCAAGTTCATCATCCGATACGCACTGAGTGGGAGAGCCTGGGATCTCGTATTTGAGCAGAGGATTGCCTTCACAAGAAAGAAGCATTTCGGAAAGGAAGAAAGGGAGCTTAGATGGGCACACGCGCAGAGGACTCTCCACGGTCTGCAACCGCCGGACAGCAAAAAGTTTGGTGAACGCAGTTCTTACACAGAACTTAATCAGATGGCTGAAGAGGCAAAAAGACGTGCAGAAATTGCCAGGCTGAGGGAGTTCGGCACCCTGAAGGGGCATGTCGAATCAGTGGTGAGGATGAAGGGCCTGGACATCGACACAATTCAGCAATCGTACACTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

957

Amino Acids

105.27

Weight (kDa)

6.14

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 20 - 83 1.1e-13 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 132 - 231 5.7e-30 P-type ATPase actuator domain
Hydrolase PF00702 327 - 604 8e-16 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 376 - 495 2.8e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1582
Acc16I TGCGCA 1 cut(s) 684
Acc36I ACCTGC 2 cut(s) 849, 2307
AccB1I GGYRCC 2 cut(s) 1459, 2789
AccB7I CCANNNNNTGG 1 cut(s) 922
AccII CGCG 2 cut(s) 2453, 2657
AciI CCGC 7 cut(s) 638, 823, 926, 1652, 2132, 2453, 2684
AclWI GGATC 7 cut(s) 314, 440, 521, 1157, 1374, 1466, 2583
AcoI YGGCCR 2 cut(s) 249, 1158
AcsI RAATTY 2 cut(s) 745, 864
AcuI CTGAAG 2 cut(s) 2763, 2816
AcyI GRCGYC 1 cut(s) 1579
AdeI CACNNNGTG 1 cut(s) 2561
AfeI AGCGCT 1 cut(s) 1903
AfiI CCNNNNNNNGG 5 cut(s) 147, 922, 925, 2778, 2799
AflIII ACRYGT 1 cut(s) 1602
AgeI ACCGGT 2 cut(s) 918, 2147
AhdI GACNNNNNGTC 1 cut(s) 1578
AhlI ACTAGT 1 cut(s) 1913
AjiI CACGTC 1 cut(s) 2759
Alw21I GWGCWC 5 cut(s) 60, 1200, 1288, 1314, 1716
Alw26I GTCTC 6 cut(s) 398, 483, 518, 1490, 1565, 1589
Alw44I GTGCAC 3 cut(s) 1196, 1284, 1310
AlwI GGATC 7 cut(s) 314, 440, 521, 1157, 1374, 1466, 2583
AlwNI CAGNNNCTG 2 cut(s) 136, 1880
Ama87I CYCGRG 3 cut(s) 1101, 2340, 2363
Aor51HI AGCGCT 1 cut(s) 1903
AoxI GGCC 6 cut(s) 138, 249, 1158, 1436, 1882, 2830
ApaLI GTGCAC 3 cut(s) 1196, 1284, 1310
ApoI RAATTY 2 cut(s) 745, 864
AsiGI ACCGGT 2 cut(s) 918, 2147
Asp700I GAANNNNTTC 2 cut(s) 1855, 2618
AspLEI GCGC 4 cut(s) 685, 1456, 1904, 2659
AspS9I GGNCC 3 cut(s) 139, 1436, 2830
AsuC2I CCSGG 3 cut(s) 1111, 2341, 2342
AvaI CYCGRG 3 cut(s) 1101, 2340, 2363
BaeGI GKGCMC 5 cut(s) 1200, 1288, 1314, 2347, 2653
BaeI ACNNNNGTAYC 2 cut(s) 1071, 1104
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 2 cut(s) 1459, 2789
BbsI GAAGAC 1 cut(s) 2503
Bbv12I GWGCWC 5 cut(s) 60, 1200, 1288, 1314, 1716
BbvCI CCTCAGC 1 cut(s) 2777
BceAI ACGGC 1 cut(s) 972
BcgI CGANNNNNNTGC 4 cut(s) 425, 459, 1631, 1665
BclI TGATCA 4 cut(s) 541, 777, 1549, 2043
BcnI CCSGG 3 cut(s) 1111, 2341, 2342
BcoDI GTCTC 6 cut(s) 398, 483, 518, 1490, 1565, 1589
BcuI ACTAGT 1 cut(s) 1913
BfaI CTAG 4 cut(s) 260, 1385, 1914, 2129
BfmI CTRYAG 3 cut(s) 237, 2272, 2289
BfoI RGCGCY 1 cut(s) 1905
BfuAI ACCTGC 2 cut(s) 849, 2307
BglII AGATCT 1 cut(s) 2136
BlpI GCTNAGC 2 cut(s) 126, 2406
BmeRI GACNNNNNGTC 1 cut(s) 1578
BmeT110I CYCGRG 3 cut(s) 1101, 2340, 2363
BmgBI CACGTC 1 cut(s) 2759
BmgT120I GGNCC 3 cut(s) 139, 1436, 2830
BmiI GGNNCC 5 cut(s) 212, 913, 1461, 2241, 2791
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 9 cut(s) 433, 496, 1115, 1117, 1126, 1276, 1777, 1822, 1831
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 1 cut(s) 2503
BpmI CTGGAG 2 cut(s) 267, 1719
Bpu10I CCTNAGC 2 cut(s) 1710, 2777
Bpu1102I GCTNAGC 2 cut(s) 126, 2406
BpuEI CTTGAG 3 cut(s) 109, 182, 2519
BpuMI CCSGG 3 cut(s) 1111, 2341, 2342
BsaAI YACGTR 2 cut(s) 61, 2348
BsaBI GATNNNNATC 2 cut(s) 525, 1378
BsaHI GRCGYC 1 cut(s) 1579
BsaJI CCNNGG 9 cut(s) 408, 586, 826, 1429, 1439, 2158, 2340, 2571, 2671
BsaWI WCCGGW 2 cut(s) 918, 2147
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 922, 925, 2778, 2799
Bse118I RCCGGY 2 cut(s) 918, 2147
Bse1I ACTGG 5 cut(s) 658, 1009, 1661, 1774, 2004
Bse3DI GCAATG 6 cut(s) 252, 645, 870, 1449, 2046, 2424
Bse8I GATNNNNATC 2 cut(s) 525, 1378
BseDI CCNNGG 9 cut(s) 408, 586, 826, 1429, 1439, 2158, 2340, 2571, 2671
BseGI GGATG 9 cut(s) 10, 448, 957, 988, 1106, 1770, 2154, 2544, 2829
BseJI GATNNNNATC 2 cut(s) 525, 1378
BseLI CCNNNNNNNGG 5 cut(s) 147, 922, 925, 2778, 2799
BseMI GCAATG 6 cut(s) 252, 645, 870, 1449, 2046, 2424
BseMII CTCAG 6 cut(s) 108, 140, 1701, 2420, 2549, 2768
BseNI ACTGG 5 cut(s) 658, 1009, 1661, 1774, 2004
BseRI GAGGAG 5 cut(s) 179, 285, 452, 506, 1467
BseSI GKGCMC 5 cut(s) 1200, 1288, 1314, 2347, 2653
BsgI GTGCAG 2 cut(s) 1512, 2781
Bsh1236I CGCG 2 cut(s) 2453, 2657
BshFI GGCC 6 cut(s) 140, 251, 1160, 1438, 1884, 2832
BshNI GGYRCC 2 cut(s) 1459, 2789
BshTI ACCGGT 2 cut(s) 918, 2147
BsiHKAI GWGCWC 5 cut(s) 60, 1200, 1288, 1314, 1716
BsiHKCI CYCGRG 3 cut(s) 1101, 2340, 2363
BsiSI CCGG 5 cut(s) 919, 1111, 2148, 2341, 2687
BslFI GGGAC 2 cut(s) 1021, 1641
BslI CCNNNNNNNGG 5 cut(s) 147, 922, 925, 2778, 2799
BsmAI GTCTC 6 cut(s) 398, 483, 518, 1490, 1565, 1589
BsmBI CGTCTC 4 cut(s) 398, 518, 1490, 1589
BsmFI GGGAC 2 cut(s) 1021, 1641
BsnI GGCC 6 cut(s) 140, 251, 1160, 1438, 1884, 2832
BsoBI CYCGRG 3 cut(s) 1101, 2340, 2363
Bsp1286I GDGCHC 7 cut(s) 60, 1200, 1288, 1314, 1716, 2347, 2653
Bsp1720I GCTNAGC 2 cut(s) 126, 2406
BspACI CCGC 7 cut(s) 638, 823, 926, 1652, 2132, 2453, 2684
BspANI GGCC 6 cut(s) 140, 251, 1160, 1438, 1884, 2832
BspCNI CTCAG 6 cut(s) 109, 139, 1702, 2419, 2550, 2769
BspFNI CGCG 2 cut(s) 2453, 2657
BspLI GGNNCC 5 cut(s) 212, 913, 1461, 2241, 2791
BspMAI CTGCAG 2 cut(s) 241, 2293
BspMI ACCTGC 2 cut(s) 849, 2307
BspPI GGATC 7 cut(s) 314, 440, 521, 1157, 1374, 1466, 2583
BspT107I GGYRCC 2 cut(s) 1459, 2789
BsrDI GCAATG 6 cut(s) 252, 645, 870, 1449, 2046, 2424
BsrFI RCCGGY 2 cut(s) 918, 2147
BsrI ACTGG 5 cut(s) 658, 1009, 1661, 1774, 2004
BssAI RCCGGY 2 cut(s) 918, 2147
BssECI CCNNGG 9 cut(s) 408, 586, 826, 1429, 1439, 2158, 2340, 2571, 2671
BssNI GRCGYC 1 cut(s) 1579
BssT1I CCWWGG 3 cut(s) 408, 1429, 1439
Bst4CI ACNGT 9 cut(s) 596, 886, 1033, 1084, 1490, 2120, 2185, 2675, 2866
Bst6I CTCTTC 3 cut(s) 66, 345, 2739
BstACI GRCGYC 1 cut(s) 1579
BstAPI GCANNNNNTGC 3 cut(s) 1136, 1841, 2768
BstBAI YACGTR 2 cut(s) 61, 2348
BstC8I GCNNGC 5 cut(s) 138, 976, 1557, 1743, 2283
BstDSI CCRYGG 1 cut(s) 2671
BstF5I GGATG 9 cut(s) 10, 448, 957, 988, 1106, 1770, 2154, 2544, 2829
BstFNI CGCG 2 cut(s) 2453, 2657
BstH2I RGCGCY 1 cut(s) 1905
BstHHI GCGC 4 cut(s) 685, 1456, 1904, 2659
BstMAI GTCTC 6 cut(s) 398, 483, 518, 1490, 1565, 1589
BstNSI RCATGY 3 cut(s) 618, 1606, 2807
BstSFI CTRYAG 3 cut(s) 237, 2272, 2289
BstSLI GKGCMC 5 cut(s) 1200, 1288, 1314, 2347, 2653
BstUI CGCG 2 cut(s) 2453, 2657
BstV2I GAAGAC 1 cut(s) 2503
BstX2I RGATCY 3 cut(s) 1379, 2136, 2575
BstXI CCANNNNNNTGG 2 cut(s) 769, 2121
BstYI RGATCY 3 cut(s) 1379, 2136, 2575
BsuRI GGCC 6 cut(s) 140, 251, 1160, 1438, 1884, 2832
BtgI CCRYGG 1 cut(s) 2671
BtgZI GCGATG 1 cut(s) 748
BtrI CACGTC 1 cut(s) 2759
BtsCI GGATG 9 cut(s) 10, 448, 957, 988, 1106, 1770, 2154, 2544, 2829
BtsI GCAGTG 2 cut(s) 1000, 2298
BtsIMutI CAGTG 8 cut(s) 601, 651, 1000, 1495, 2298, 2555, 2820, 2862
BveI ACCTGC 2 cut(s) 849, 2307
Cac8I GCNNGC 5 cut(s) 138, 976, 1557, 1743, 2283
CaiI CAGNNNCTG 2 cut(s) 136, 1880
CfoI GCGC 4 cut(s) 685, 1456, 1904, 2659
Cfr10I RCCGGY 2 cut(s) 918, 2147
Cfr13I GGNCC 3 cut(s) 139, 1436, 2830
Cfr9I CCCGGG 1 cut(s) 2340
CsiI ACCWGGT 1 cut(s) 2371
CspAI ACCGGT 2 cut(s) 918, 2147
DraIII CACNNNGTG 1 cut(s) 2561
DriI GACNNNNNGTC 1 cut(s) 1578
EaeI YGGCCR 2 cut(s) 249, 1158
Eam1104I CTCTTC 3 cut(s) 66, 345, 2739
Eam1105I GACNNNNNGTC 1 cut(s) 1578
EarI CTCTTC 3 cut(s) 66, 345, 2739
EciI GGCGGA 1 cut(s) 941
Eco130I CCWWGG 3 cut(s) 408, 1429, 1439
Eco32I GATATC 1 cut(s) 2530
Eco47III AGCGCT 1 cut(s) 1903
Eco57I CTGAAG 2 cut(s) 2763, 2816
Eco88I CYCGRG 3 cut(s) 1101, 2340, 2363
EcoO109I RGGNCCY 2 cut(s) 1436, 2830
EcoRI GAATTC 1 cut(s) 864
EcoRV GATATC 1 cut(s) 2530
EcoT14I CCWWGG 3 cut(s) 408, 1429, 1439
EcoT22I ATGCAT 1 cut(s) 1269
ErhI CCWWGG 3 cut(s) 408, 1429, 1439
Esp3I CGTCTC 4 cut(s) 398, 518, 1490, 1589
FaqI GGGAC 2 cut(s) 1021, 1641
FauI CCCGC 1 cut(s) 1645
FauNDI CATATG 1 cut(s) 2009
FbaI TGATCA 4 cut(s) 541, 777, 1549, 2043
FokI GGATG 9 cut(s) 17, 455, 964, 995, 1093, 1777, 2141, 2531, 2836
FspBI CTAG 4 cut(s) 260, 1385, 1914, 2129
FspI TGCGCA 1 cut(s) 684
GlaI GCGC 4 cut(s) 684, 1455, 1903, 2658
GsuI CTGGAG 2 cut(s) 267, 1719
HaeII RGCGCY 1 cut(s) 1905
HaeIII GGCC 6 cut(s) 140, 251, 1160, 1438, 1884, 2832
HapII CCGG 5 cut(s) 919, 1111, 2148, 2341, 2687
HhaI GCGC 4 cut(s) 685, 1456, 1904, 2659
Hin1I GRCGYC 1 cut(s) 1579
Hin6I GCGC 4 cut(s) 683, 1454, 1902, 2657
HinP1I GCGC 4 cut(s) 683, 1454, 1902, 2657
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HindIII AAGCTT 3 cut(s) 158, 1025, 2279
HpaII CCGG 5 cut(s) 919, 1111, 2148, 2341, 2687
Hpy166II GTNNAC 8 cut(s) 1036, 1072, 1198, 1286, 1312, 2296, 2708, 2862
Hpy188I TCNGA 9 cut(s) 118, 1014, 1324, 1506, 1861, 2043, 2549, 2624, 2736
Hpy188III TCNNGA 8 cut(s) 284, 419, 730, 1124, 1190, 1409, 1930, 2363
Hpy8I GTNNAC 8 cut(s) 1036, 1072, 1198, 1286, 1312, 2296, 2708, 2862
Hpy99I CGWCG 1 cut(s) 1584
HpyCH4III ACNGT 9 cut(s) 596, 886, 1033, 1084, 1490, 2120, 2185, 2675, 2866
HpyCH4IV ACGT 5 cut(s) 60, 1074, 1579, 2347, 2758
HpySE526I ACGT 5 cut(s) 60, 1074, 1579, 2347, 2758
Hsp92I GRCGYC 1 cut(s) 1579
HspAI GCGC 4 cut(s) 683, 1454, 1902, 2657
Ksp22I TGATCA 4 cut(s) 541, 777, 1549, 2043
LmnI GCTCC 5 cut(s) 55, 403, 928, 1795, 2638
LweI GCATC 9 cut(s) 433, 496, 1115, 1117, 1126, 1276, 1777, 1822, 1831
MabI ACCWGGT 1 cut(s) 2371
MaeI CTAG 4 cut(s) 260, 1385, 1914, 2129
MaeII ACGT 5 cut(s) 60, 1074, 1579, 2347, 2758
MaeIII GTNAC 9 cut(s) 95, 564, 574, 590, 818, 895, 995, 2228, 2335
MfeI CAATTG 2 cut(s) 738, 960
MflI RGATCY 3 cut(s) 1379, 2136, 2575
MhlI GDGCHC 7 cut(s) 60, 1200, 1288, 1314, 1716, 2347, 2653
MlsI TGGCCA 1 cut(s) 251
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 5 cut(s) 102, 572, 1003, 1036, 2659
MmeI TCCRAC 1 cut(s) 2102
Mox20I TGGCCA 1 cut(s) 251
Mph1103I ATGCAT 1 cut(s) 1269
MroXI GAANNNNTTC 2 cut(s) 1855, 2618
MscI TGGCCA 1 cut(s) 251
MseI TTAA 7 cut(s) 480, 1062, 1376, 1533, 1782, 1800, 2730
MslI CAYNNNNRTG 7 cut(s) 767, 794, 875, 1488, 1719, 1758, 2008
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 2410
MspI CCGG 5 cut(s) 919, 1111, 2148, 2341, 2687
MunI CAATTG 2 cut(s) 738, 960
MvnI CGCG 2 cut(s) 2453, 2657
NciI CCSGG 3 cut(s) 1111, 2341, 2342
NdeI CATATG 1 cut(s) 2009
NlaIV GGNNCC 5 cut(s) 212, 913, 1461, 2241, 2791
NmuCI GTSAC 8 cut(s) 95, 564, 574, 590, 895, 995, 2228, 2335
NsbI TGCGCA 1 cut(s) 684
NsiI ATGCAT 1 cut(s) 1269
NspI RCATGY 3 cut(s) 618, 1606, 2807
PaeR7I CTCGAG 2 cut(s) 1101, 2363
PciI ACATGT 1 cut(s) 1602
PdmI GAANNNNTTC 2 cut(s) 1855, 2618
PfeI GAWTC 6 cut(s) 703, 1303, 1501, 1516, 1643, 2810
PflFI GACNNNGTC 2 cut(s) 1581, 2373
PflMI CCANNNNNTGG 1 cut(s) 922
PfoI TCCNGGA 1 cut(s) 1109
PinAI ACCGGT 2 cut(s) 918, 2147
PleI GAGTC 5 cut(s) 102, 571, 1003, 1036, 2659
PpsI GAGTC 5 cut(s) 102, 571, 1003, 1036, 2659
Ppu21I YACGTR 2 cut(s) 61, 2348
PscI ACATGT 1 cut(s) 1602
PspN4I GGNNCC 5 cut(s) 212, 913, 1461, 2241, 2791
PspPI GGNCC 3 cut(s) 139, 1436, 2830
PspXI VCTCGAGB 1 cut(s) 1101
PsrI GAACNNNNNNTAC 2 cut(s) 1222, 1254
PstI CTGCAG 2 cut(s) 241, 2293
PstNI CAGNNNCTG 2 cut(s) 136, 1880
PsuI RGATCY 3 cut(s) 1379, 2136, 2575
PsyI GACNNNGTC 2 cut(s) 1581, 2373
PvuII CAGCTG 1 cut(s) 2410
RseI CAYNNNNRTG 7 cut(s) 767, 794, 875, 1488, 1719, 1758, 2008
SaqAI TTAA 7 cut(s) 480, 1062, 1376, 1533, 1782, 1800, 2730
Sau96I GGNCC 3 cut(s) 139, 1436, 2830
SchI GAGTC 5 cut(s) 102, 572, 1003, 1036, 2659
SduI GDGCHC 7 cut(s) 60, 1200, 1288, 1314, 1716, 2347, 2653
SexAI ACCWGGT 1 cut(s) 2371
SfaNI GCATC 9 cut(s) 433, 496, 1115, 1117, 1126, 1276, 1777, 1822, 1831
SfcI CTRYAG 3 cut(s) 237, 2272, 2289
Sfr274I CTCGAG 2 cut(s) 1101, 2363
SlaI CTCGAG 2 cut(s) 1101, 2363
SmaI CCCGGG 1 cut(s) 2342
SmiMI CAYNNNNRTG 7 cut(s) 767, 794, 875, 1488, 1719, 1758, 2008
SmlI CTYRAG 5 cut(s) 88, 161, 1101, 2363, 2534
SmoI CTYRAG 5 cut(s) 88, 161, 1101, 2363, 2534
SpeI ACTAGT 1 cut(s) 1913
SsiI CCGC 7 cut(s) 638, 823, 926, 1652, 2132, 2453, 2684
SspI AATATT 1 cut(s) 1926
SspMI CTAG 4 cut(s) 260, 1385, 1914, 2129
StyI CCWWGG 3 cut(s) 408, 1429, 1439
TaaI ACNGT 9 cut(s) 596, 886, 1033, 1084, 1490, 2120, 2185, 2675, 2866
TaiI ACGT 5 cut(s) 63, 1077, 1582, 2350, 2761
TatI WGTACW 2 cut(s) 1867, 1906
TauI GCSGC 1 cut(s) 2456
TfiI GAWTC 6 cut(s) 703, 1303, 1501, 1516, 1643, 2810
Tru1I TTAA 7 cut(s) 480, 1062, 1376, 1533, 1782, 1800, 2730
Tru9I TTAA 7 cut(s) 480, 1062, 1376, 1533, 1782, 1800, 2730
TscAI CASTG 8 cut(s) 601, 658, 1000, 1495, 2298, 2562, 2820, 2869
TseFI GTSAC 8 cut(s) 95, 564, 574, 590, 895, 995, 2228, 2335
Tsp45I GTSAC 8 cut(s) 95, 564, 574, 590, 895, 995, 2228, 2335
TspGWI ACGGA 3 cut(s) 964, 1197, 1964
TspMI CCCGGG 1 cut(s) 2340
TspRI CASTG 8 cut(s) 601, 658, 1000, 1495, 2298, 2562, 2820, 2869
Tth111I GACNNNGTC 2 cut(s) 1581, 2373
Van91I CCANNNNNTGG 1 cut(s) 922
VneI GTGCAC 3 cut(s) 1196, 1284, 1310
XapI RAATTY 2 cut(s) 745, 864
XceI RCATGY 3 cut(s) 618, 1606, 2807
XhoI CTCGAG 2 cut(s) 1101, 2363
XmaI CCCGGG 1 cut(s) 2340
XmnI GAANNNNTTC 2 cut(s) 1855, 2618
XspI CTAG 4 cut(s) 260, 1385, 1914, 2129
ZraI GACGTC 1 cut(s) 1580
Zsp2I ATGCAT 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.