Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0002286

Physical Location & Seq

Genomic Coordinates Forward (+)

2412841 .. 2419976

Gene Structure 7136 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG035400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2667 bp

ATGTGGAATCCTCTCTCATGGGTTATGGAAGCTGCTGCTATAATGGCCATTGCGCTTGCAAATGGAGGAGGCAAGCCTCCTGACTGGCAAGATTTTGTGGGTATTATTACTCTGCTGGTCATCAACTCCACCATTAGTTTCATCGAGGAAAATAATGCGGGTAATGCTGCAGCAGCTCTCATGGCTCGTCTTGCTCCTAAAGCCAAGGTTCTTCGAGATGGAAGGTGGAACGAGCAAGATGCTGCTCTCCTAGTACCAGGTGACATAATTAGTATCAAACTCGGTGATATTGTTCCAGCTGATGCACGGCTACTTGAAGGGGATCCATTGAAAATTGATCAGTCTGCACTTACAGGCGAGTCTCTTCCTGTGACAAAAGGCCCCGGGGATGGTGTGTACTCTGGTTCAACTTGCAAACAAGGAGAGATTGAAGCAGTGGTCATTGCCACCGGAGTGCATACCTTCTTTGGGAAGGCTGCTCACCTCGTTGATAGCACTAACCAAGTGGGCCACTTCCAAAAGGTCTTAACTGCAATAGGGAATTTCTGCATATGCTCTATTGCTGTGGGTATGGTAATAGAGATAATTGTCATGTACCCGATTCAAGACCGAGAGTATCGCCCTGGAATTGACAATCTTCTTGTGCTTCTCATTGGTGGAATTCCAATTGCAATGCCCACAGTTCTGTCCGTGACAATGGCAATTGGCTCTCATAGATTAGCACAGCAGGGAGCTATCACAAAGAGAATGACAGCAATTGAAGAGATGGCGGGCATGGATGTGCTTTGCAGTGATAAAACTGGAACTCTAACACTGAACAAGCTTACAGTTGATAAAAATCTTATTGAGGTTTTTGCAAAAGGAGTGGATCCGGATACTGTTGTTCTGATGGCAGCTCGAGCATCCAGGATAGAGAATCAAGATGCAATAGATACTGCTATAGTTGGGATGCTGGCTGATCCTAAGGAGGCACGTGCTGGCGTTCAAGAAATACACTTCCTTCCTTTCAATCCTACTGATAAGCGAACTGCATTGACTTATATTGACCGTGATGGCAAAATGCATAGAGTTAGCAAAGGTGCACCAGAGCAGATTCTACATCTTGCCCACAATAAGTCGGACATTGAGCGTAGAGTTCATGCTGTGATTGATAAGTTTGCTGAGCGAGGGTTACGGTCTCTTGCTGTAGCATACCAGGAAGTTCCAGAAGGAATCAAAGAGGGTGCTGGAGGCCCGTGGCAATTTATTGGTCTAATGCCACTCTTTGACCCACCTAGGCATGACAGTGCAGAGACAATACGGAGGGCTTTAAATCTTGGAGTAAATGTGAAAATGATTACAGGTGATCAACTGGCCATAGGAAAGGAGACTGGACGTCGTTTGGGAATGGGAACCAATATGTATCCTTCATCTGCTTTACTAGGACAGGACAAGGATGAATCGATTGCGGCTTTACCCATTGATGATCTGATAGAAAAAGCCGATGGCTTTGCTGGTGTTTTCCCAGAGCACAAATACGAGATTGTAAAACGCTTGCAAGCAAGGAAGCATATCTGTGGAATGACCGGTGATGGAGTTAATGATGCTCCTGCCCTCAAAAAAGCCGATATTGGTATTGCTGTTGCTGATGCAACAGATGCAGCCCGTAGTGCTTCTGATATTGTGCTAACTGAACCTGGCCTTAGTGTTATCATTAGTGCTGTTTTGACTAGTCGGGCAATCTTCCAGAGGATGAAAAATTACACAATATATGCTGTTTCCATCACAATTCGTATTGTGATGGGTTTCATGTTGCTGGCACTCATATGGAAGTTCGACTTTCCACCTTTCATGGTGCTTATTATTGCAATCCTCAATGATGGTACCATCATGACGATATCAAAGGATAGGGTTAAACCATCTCCTCTGCCAGATAGCTGGAAGCTGTCGGAGATTTTTGCAACTGGAATTGTGCTCGGTACCTACTTGGCAATAATGACTGTAATATTCTTTTGGGCAGCATACAAAACAGACTTCTTCCCAAGAATATTTGGGGTATCAACCCTTGAGAAGACAGCTCATGATGACTTTCGGAAACTTGCCTCTGCTATATATCTACAAGTGAGCATCATTAGTCAGGCCCTCATTTTTGTTACACGCTCTCGAAGTTGGTCCTTTGTTGAGCGGCCTGGAATGTTGCTTGTTGTGGCTTTCCTGATAGCCCAGCTGATTGCCACCTTGATAGCAGTGTATGCTAACTGGGAGTTTGCTGCAATCGAAGGGATTGGATGGGGTTGGGCTGGGGTGATCTGGCTTTATAACATCATCTTCTATATCCCTCTGGATATCATAAAGTTCATGATACGCTATGCTCTGAGTGGGAAGGCTTGGGATCTTCTCATTGAGCAAAGGATTGCTTTCACAAGGCAAAAAGACTTTGGAAAGGAACAGCGTGAGCTTCAATGGGCACATGCACAAAGAACACTGCATGGTTTGCAACCACCAGATACCAAGATGTTTACTGAAAGAACACATTTCACAGAACTCAATCAGATGGCTGAAGAAGCCAAAAGAAGAGCTGAAATTGCAAGGTTGAGAGAACTGCATACACTAAAAGGTCACGTAGAGTCAGTGGTGAGATTGAAGGGTCTCGACATCGACACAATTCAGCAAGCATACACAGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

888

Amino Acids

97.57

Weight (kDa)

6.44

Isoelectric Point (pI)

36.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	64 - 163	9.9e-31	P-type ATPase actuator domain
Hydrolase	PF00702	259 - 536	3.2e-13	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	307 - 427	8.2e-06	P-type ATPase, cytoplasmic domain N

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002286)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47950 AT3G47950 AT5G62670
fragaria_vesca	FvH4_5g15700 FvH4_5g15700 FvH4_5g15700 FvH4_5g15700 FvH4_6g49360
malus_domestica	MD06G1186400.v1.1 MD14G1192600.v1.1 MD17G1046600.v1.1
prunus_persica	Prupe.3G272500_v2.0.a1 Prupe.3G272500_v2.0.a1 Prupe.3G272500_v2.0.a1 Prupe.3G272500_v2.0.a1 Prupe.5G185000_v2.0.a1
pyrus_communis	pycom06g16560 pycom06g16570 pycom14g16020 pycom17g04150
rosa_chinensis	RchiOBHm_Chr2g0169231 RchiOBHm_Chr7g0180411
rosa_laevigata	RLG00000005268 RLG00000021844
rosa_multiflora	Rmu_co8446409.1_g000001 Rmu_sc0003526.1_g000005 Rmu_ssc0000440.1_g000009
rosa_roxburghii	Rroxscaffold_2G00082330 Rroxscaffold_3G00272910
rosa_rugosa	Rorug02G0538400 Rorug06G0432800 Rorug06G0432900 Rorug06G0432900
rosa_samantha	Rh2AG609900 Rh2BG622800 Rh2CG591300 Rh7AG033500 Rh7BG033500 Rh7CG035400 Rh7DG033900
rosa_wichuraiana	Rw2G050570 Rw7G002800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2298
AatII	GACGTC	1 cut(s)	1378
Acc65I	GGTACC	2 cut(s)	1862, 1958
AccB1I	GGYRCC	2 cut(s)	1862, 1958
AccBSI	CCGCTC	1 cut(s)	2164
AccIII	TCCGGA	1 cut(s)	871
AciI	CCGC	4 cut(s)	158, 770, 1448, 2164
AclWI	GGATC	6 cut(s)	317, 330, 863, 876, 953, 2379
AcoI	YGGCCR	2 cut(s)	45, 1353
AcsI	RAATTY	2 cut(s)	541, 660
AcuI	CTGAAG	1 cut(s)	2559
AcvI	CACGTG	1 cut(s)	974
AcyI	GRCGYC	1 cut(s)	1375
AfaI	GTAC	5 cut(s)	255, 398, 596, 1864, 1960
AfiI	CCNNNNNNNGG	2 cut(s)	389, 468
AgeI	ACCGGT	1 cut(s)	1565
AhdI	GACNNNNNGTC	1 cut(s)	1374
AhlI	ACTAGT	1 cut(s)	1709
AjnI	CCWGG	6 cut(s)	256, 622, 905, 1194, 1675, 2167
AleI	CACNNNNGTG	1 cut(s)	452
Alw21I	GWGCWC	3 cut(s)	1084, 1512, 1956
Alw26I	GTCTC	5 cut(s)	366, 1182, 1286, 1361, 2633
Alw44I	GTGCAC	1 cut(s)	1080
AlwI	GGATC	6 cut(s)	317, 330, 863, 876, 953, 2379
Ama87I	CYCGRG	2 cut(s)	383, 897
Aor13HI	TCCGGA	1 cut(s)	871
AoxI	GGCC	8 cut(s)	45, 379, 508, 1231, 1353, 1678, 2118, 2165
ApaLI	GTGCAC	1 cut(s)	1080
ApoI	RAATTY	2 cut(s)	541, 660
AsiGI	ACCGGT	1 cut(s)	1565
Asp718I	GGTACC	2 cut(s)	1862, 1958
AspA2I	CCTAGG	1 cut(s)	1274
AspLEI	GCGC	1 cut(s)	55
AspS9I	GGNCC	5 cut(s)	380, 508, 1232, 2119, 2151
AsuC2I	CCSGG	2 cut(s)	384, 385
AsuHPI	GGTGA	7 cut(s)	272, 296, 473, 1355, 1580, 2296, 2626
AvaI	CYCGRG	2 cut(s)	383, 897
AvaII	GGWCC	1 cut(s)	2151
AvrII	CCTAGG	1 cut(s)	1274
AxyI	CCTNAGG	1 cut(s)	963
BaeGI	GKGCMC	2 cut(s)	1084, 2449
BaeI	ACNNNNGTAYC	2 cut(s)	867, 900
BalI	TGGCCA	2 cut(s)	47, 1355
BamHI	GGATCC	2 cut(s)	322, 868
BanI	GGYRCC	2 cut(s)	1862, 1958
BbrPI	CACGTG	1 cut(s)	974
BbsI	GAAGAC	1 cut(s)	2057
Bbv12I	GWGCWC	3 cut(s)	1084, 1512, 1956
BceAI	ACGGC	1 cut(s)	323
BcgI	CGANNNNNNTGC	4 cut(s)	221, 255, 1472, 1506
BciT130I	CCWGG	6 cut(s)	258, 624, 907, 1196, 1677, 2169
BciVI	GTATCC	2 cut(s)	868, 1413
BclI	TGATCA	2 cut(s)	337, 1345
BcnI	CCSGG	2 cut(s)	384, 385
BcoDI	GTCTC	5 cut(s)	366, 1182, 1286, 1361, 2633
BcuI	ACTAGT	1 cut(s)	1709
BfaI	CTAG	5 cut(s)	251, 1275, 1421, 1710, 2665
BfmI	CTRYAG	3 cut(s)	168, 939, 1185
BfuI	GTATCC	2 cut(s)	868, 1413
BlnI	CCTAGG	1 cut(s)	1274
BlpI	GCTNAGC	1 cut(s)	1161
Bme1390I	CCNGG	8 cut(s)	258, 384, 385, 624, 907, 1196, 1677, 2169
Bme18I	GGWCC	1 cut(s)	2151
BmeRI	GACNNNNNGTC	1 cut(s)	1374
BmeT110I	CYCGRG	2 cut(s)	383, 897
BmgT120I	GGNCC	5 cut(s)	380, 508, 1232, 2119, 2151
BmiI	GGNNCC	6 cut(s)	324, 382, 870, 1393, 1864, 1960
BmrFI	CCNGG	8 cut(s)	258, 384, 385, 624, 907, 1196, 1677, 2169
BmrI	ACTGGG	1 cut(s)	2248
BmsI	GCATC	9 cut(s)	229, 292, 911, 913, 939, 1573, 1618, 1627, 2115
BmuI	ACTGGG	1 cut(s)	2248
BpiI	GAAGAC	1 cut(s)	2057
BpmI	CTGGAG	1 cut(s)	1248
Bpu1102I	GCTNAGC	1 cut(s)	1161
BpuEI	CTTGAG	1 cut(s)	2066
BpuMI	CCSGG	2 cut(s)	384, 385
Bsa29I	ATCGAT	1 cut(s)	1442
BsaAI	YACGTR	2 cut(s)	974, 2602
BsaBI	GATNNNNATC	1 cut(s)	837
BsaHI	GRCGYC	1 cut(s)	1375
BsaI	GGTCTC	2 cut(s)	1182, 2633
BsaJI	CCNNGG	7 cut(s)	204, 382, 383, 384, 622, 1235, 1274
BsaWI	WCCGGW	3 cut(s)	449, 871, 1565
BsaXI	ACNNNNNCTCC	2 cut(s)	1311, 1341
Bsc4I	CCNNNNNNNGG	2 cut(s)	389, 468
Bse118I	RCCGGY	1 cut(s)	1565
Bse1I	ACTGG	6 cut(s)	89, 805, 1356, 1375, 1948, 2243
Bse21I	CCTNAGG	1 cut(s)	963
Bse3DI	GCAATG	3 cut(s)	48, 441, 678
Bse8I	GATNNNNATC	1 cut(s)	837
BseAI	TCCGGA	1 cut(s)	871
BseBI	CCWGG	6 cut(s)	258, 624, 907, 1196, 1677, 2169
BseCI	ATCGAT	1 cut(s)	1442
BseDI	CCNNGG	7 cut(s)	204, 382, 383, 384, 622, 1235, 1274
BseGI	GGATG	7 cut(s)	394, 784, 902, 954, 1441, 1737, 2273
BseJI	GATNNNNATC	1 cut(s)	837
BseLI	CCNNNNNNNGG	2 cut(s)	389, 468
BseMI	GCAATG	3 cut(s)	48, 441, 678
BseMII	CTCAG	2 cut(s)	1152, 2345
BseNI	ACTGG	6 cut(s)	89, 805, 1356, 1375, 1948, 2243
BseRI	GAGGAG	2 cut(s)	81, 1893
BseSI	GKGCMC	2 cut(s)	1084, 2449
BseYI	CCCAGC	2 cut(s)	2202, 2279
BsgI	GTGCAG	2 cut(s)	330, 1308
BshFI	GGCC	8 cut(s)	47, 381, 510, 1233, 1355, 1680, 2120, 2167
BshNI	GGYRCC	2 cut(s)	1862, 1958
BshTI	ACCGGT	1 cut(s)	1565
BshVI	ATCGAT	1 cut(s)	1442
BsiHKAI	GWGCWC	3 cut(s)	1084, 1512, 1956
BsiHKCI	CYCGRG	2 cut(s)	383, 897
BsiSI	CCGG	4 cut(s)	384, 450, 872, 1566
BslI	CCNNNNNNNGG	2 cut(s)	389, 468
BsmAI	GTCTC	5 cut(s)	366, 1182, 1286, 1361, 2633
BsnI	GGCC	8 cut(s)	47, 381, 510, 1233, 1355, 1680, 2120, 2167
Bso31I	GGTCTC	2 cut(s)	1182, 2633
BsoBI	CYCGRG	2 cut(s)	383, 897
Bsp1286I	GDGCHC	4 cut(s)	1084, 1512, 1956, 2449
Bsp13I	TCCGGA	1 cut(s)	871
Bsp143I	GATC	8 cut(s)	322, 337, 868, 958, 1345, 1465, 2286, 2371
Bsp1720I	GCTNAGC	1 cut(s)	1161
BspACI	CCGC	4 cut(s)	158, 770, 1448, 2164
BspANI	GGCC	8 cut(s)	47, 381, 510, 1233, 1355, 1680, 2120, 2167
BspCNI	CTCAG	2 cut(s)	1153, 2346
BspDI	ATCGAT	1 cut(s)	1442
BspEI	TCCGGA	1 cut(s)	871
BspHI	TCATGA	3 cut(s)	1869, 2059, 2337
BspLI	GGNNCC	6 cut(s)	324, 382, 870, 1393, 1864, 1960
BspMAI	CTGCAG	1 cut(s)	172
BspPI	GGATC	6 cut(s)	317, 330, 863, 876, 953, 2379
BspQI	GCTCTTC	1 cut(s)	2548
BspT107I	GGYRCC	2 cut(s)	1862, 1958
BspTNI	GGTCTC	2 cut(s)	1182, 2633
BsrBI	CCGCTC	1 cut(s)	2164
BsrDI	GCAATG	3 cut(s)	48, 441, 678
BsrFI	RCCGGY	1 cut(s)	1565
BsrI	ACTGG	6 cut(s)	89, 805, 1356, 1375, 1948, 2243
BssAI	RCCGGY	1 cut(s)	1565
BssECI	CCNNGG	7 cut(s)	204, 382, 383, 384, 622, 1235, 1274
BssMI	GATC	8 cut(s)	322, 337, 868, 958, 1345, 1465, 2286, 2371
BssNI	GRCGYC	1 cut(s)	1375
BssT1I	CCWWGG	2 cut(s)	204, 1274
Bst2UI	CCWGG	6 cut(s)	258, 624, 907, 1196, 1677, 2169
Bst4CI	ACNGT	8 cut(s)	682, 829, 880, 1049, 1176, 1286, 1981, 2662
Bst6I	CTCTTC	3 cut(s)	369, 756, 2548
BstACI	GRCGYC	1 cut(s)	1375
BstAPI	GCANNNNNTGC	3 cut(s)	1637, 2231, 2473
BstBAI	YACGTR	2 cut(s)	974, 2602
BstC8I	GCNNGC	9 cut(s)	57, 74, 772, 954, 979, 1535, 1539, 1797, 2652
BstDEI	CTNAG	4 cut(s)	963, 1161, 1682, 2354
BstDSI	CCRYGG	1 cut(s)	1235
BstF5I	GGATG	7 cut(s)	394, 784, 902, 954, 1441, 1737, 2273
BstHHI	GCGC	1 cut(s)	55
BstKTI	GATC	8 cut(s)	325, 340, 871, 961, 1348, 1468, 2289, 2374
BstMAI	GTCTC	5 cut(s)	366, 1182, 1286, 1361, 2633
BstMBI	GATC	8 cut(s)	322, 337, 868, 958, 1345, 1465, 2286, 2371
BstNI	CCWGG	6 cut(s)	258, 624, 907, 1196, 1677, 2169
BstNSI	RCATGY	1 cut(s)	2453
BstSCI	CCNGG	8 cut(s)	256, 382, 383, 622, 905, 1194, 1675, 2167
BstSFI	CTRYAG	3 cut(s)	168, 939, 1185
BstSLI	GKGCMC	2 cut(s)	1084, 2449
BstV2I	GAAGAC	1 cut(s)	2057
BstX2I	RGATCY	3 cut(s)	322, 868, 2371
BstXI	CCANNNNNNTGG	1 cut(s)	1917
BstYI	RGATCY	3 cut(s)	322, 868, 2371
Bsu15I	ATCGAT	1 cut(s)	1442
Bsu36I	CCTNAGG	1 cut(s)	963
BsuI	GTATCC	2 cut(s)	868, 1413
BsuRI	GGCC	8 cut(s)	47, 381, 510, 1233, 1355, 1680, 2120, 2167
BsuTUI	ATCGAT	1 cut(s)	1442
BtgI	CCRYGG	1 cut(s)	1235
BtsCI	GGATG	7 cut(s)	394, 784, 902, 954, 1441, 1737, 2273
BtsI	GCAGTG	4 cut(s)	441, 796, 2232, 2462
BtsIMutI	CAGTG	7 cut(s)	441, 796, 812, 1291, 2232, 2462, 2616
Cac8I	GCNNGC	9 cut(s)	57, 74, 772, 954, 979, 1535, 1539, 1797, 2652
CciI	TCATGA	3 cut(s)	1869, 2059, 2337
CfoI	GCGC	1 cut(s)	55
Cfr10I	RCCGGY	1 cut(s)	1565
Cfr13I	GGNCC	5 cut(s)	380, 508, 1232, 2119, 2151
Cfr9I	CCCGGG	1 cut(s)	383
ClaI	ATCGAT	1 cut(s)	1442
CsiI	ACCWGGT	1 cut(s)	256
Csp6I	GTAC	5 cut(s)	254, 397, 595, 1863, 1959
CspAI	ACCGGT	1 cut(s)	1565
CspCI	CAANNNNNGTGG	2 cut(s)	846, 881
CviQI	GTAC	5 cut(s)	254, 397, 595, 1863, 1959
DdeI	CTNAG	4 cut(s)	963, 1161, 1682, 2354
DpnI	GATC	8 cut(s)	324, 339, 870, 960, 1347, 1467, 2288, 2373
DpnII	GATC	8 cut(s)	322, 337, 868, 958, 1345, 1465, 2286, 2371
DraI	TTTAAA	1 cut(s)	1311
DriI	GACNNNNNGTC	1 cut(s)	1374
EaeI	YGGCCR	2 cut(s)	45, 1353
Eam1104I	CTCTTC	3 cut(s)	369, 756, 2548
Eam1105I	GACNNNNNGTC	1 cut(s)	1374
EarI	CTCTTC	3 cut(s)	369, 756, 2548
Eco130I	CCWWGG	2 cut(s)	204, 1274
Eco31I	GGTCTC	2 cut(s)	1182, 2633
Eco32I	GATATC	2 cut(s)	1878, 2326
Eco47I	GGWCC	1 cut(s)	2151
Eco57I	CTGAAG	1 cut(s)	2559
Eco72I	CACGTG	1 cut(s)	974
Eco81I	CCTNAGG	1 cut(s)	963
Eco88I	CYCGRG	2 cut(s)	383, 897
EcoO109I	RGGNCCY	2 cut(s)	380, 2119
EcoRI	GAATTC	1 cut(s)	660
EcoRII	CCWGG	6 cut(s)	256, 622, 905, 1194, 1675, 2167
EcoRV	GATATC	2 cut(s)	1878, 2326
EcoT14I	CCWWGG	2 cut(s)	204, 1274
EcoT22I	ATGCAT	1 cut(s)	1065
ErhI	CCWWGG	2 cut(s)	204, 1274
FalI	AAGNNNNNCTT	4 cut(s)	1802, 1834, 2380, 2412
FauI	CCCGC	2 cut(s)	151, 763
FauNDI	CATATG	2 cut(s)	551, 1805
FbaI	TGATCA	2 cut(s)	337, 1345
FokI	GGATG	7 cut(s)	401, 791, 889, 961, 1448, 1744, 2280
FspBI	CTAG	5 cut(s)	251, 1275, 1421, 1710, 2665
GlaI	GCGC	1 cut(s)	54
GsaI	CCCAGC	2 cut(s)	2206, 2283
GsuI	CTGGAG	1 cut(s)	1248
HaeIII	GGCC	8 cut(s)	47, 381, 510, 1233, 1355, 1680, 2120, 2167
HapII	CCGG	4 cut(s)	384, 450, 872, 1566
HhaI	GCGC	1 cut(s)	55
Hin1I	GRCGYC	1 cut(s)	1375
Hin6I	GCGC	1 cut(s)	53
HinP1I	GCGC	1 cut(s)	53
HindIII	AAGCTT	1 cut(s)	821
HinfI	GANTC	8 cut(s)	7, 359, 601, 916, 1093, 1212, 1439, 2606
HpaII	CCGG	4 cut(s)	384, 450, 872, 1566
HphI	GGTGA	7 cut(s)	272, 296, 473, 1355, 1580, 2296, 2626
Hpy166II	GTNNAC	3 cut(s)	397, 1082, 2499
Hpy188I	TCNGA	8 cut(s)	888, 1120, 1470, 1657, 1930, 2073, 2355, 2532
Hpy8I	GTNNAC	3 cut(s)	397, 1082, 2499
Hpy99I	CGWCG	1 cut(s)	1380
HpyCH4III	ACNGT	8 cut(s)	682, 829, 880, 1049, 1176, 1286, 1981, 2662
HpyCH4IV	ACGT	3 cut(s)	973, 1375, 2601
HpyF3I	CTNAG	4 cut(s)	963, 1161, 1682, 2354
HpySE526I	ACGT	3 cut(s)	973, 1375, 2601
Hsp92I	GRCGYC	1 cut(s)	1375
HspAI	GCGC	1 cut(s)	53
Kpn2I	TCCGGA	1 cut(s)	871
KpnI	GGTACC	2 cut(s)	1866, 1962
Ksp22I	TGATCA	2 cut(s)	337, 1345
Kzo9I	GATC	8 cut(s)	322, 337, 868, 958, 1345, 1465, 2286, 2371
LguI	GCTCTTC	1 cut(s)	2548
LmnI	GCTCC	3 cut(s)	199, 731, 1591
LweI	GCATC	9 cut(s)	229, 292, 911, 913, 939, 1573, 1618, 1627, 2115
MabI	ACCWGGT	1 cut(s)	256
MaeI	CTAG	5 cut(s)	251, 1275, 1421, 1710, 2665
MaeII	ACGT	3 cut(s)	973, 1375, 2601
MaeIII	GTNAC	6 cut(s)	260, 370, 691, 1170, 2131, 2597
MalI	GATC	8 cut(s)	324, 339, 870, 960, 1347, 1467, 2288, 2373
MbiI	CCGCTC	1 cut(s)	2164
MboI	GATC	8 cut(s)	322, 337, 868, 958, 1345, 1465, 2286, 2371
MfeI	CAATTG	3 cut(s)	666, 702, 756
MflI	RGATCY	3 cut(s)	322, 868, 2371
MhlI	GDGCHC	4 cut(s)	1084, 1512, 1956, 2449
MlsI	TGGCCA	2 cut(s)	47, 1355
MluNI	TGGCCA	2 cut(s)	47, 1355
MlyI	GAGTC	2 cut(s)	368, 2615
MmeI	TCCRAC	2 cut(s)	1098, 1908
Mox20I	TGGCCA	2 cut(s)	47, 1355
Mph1103I	ATGCAT	1 cut(s)	1065
MroI	TCCGGA	1 cut(s)	871
MscI	TGGCCA	2 cut(s)	47, 1355
MseI	TTAA	4 cut(s)	527, 1310, 1578, 1893
MslI	CAYNNNNRTG	5 cut(s)	452, 779, 1284, 1554, 1804
Msp20I	TGGCCA	2 cut(s)	47, 1355
MspA1I	CMGCKG	2 cut(s)	299, 2206
MspI	CCGG	4 cut(s)	384, 450, 872, 1566
MspR9I	CCNGG	8 cut(s)	258, 384, 385, 624, 907, 1196, 1677, 2169
MunI	CAATTG	3 cut(s)	666, 702, 756
MvaI	CCWGG	6 cut(s)	258, 624, 907, 1196, 1677, 2169
NciI	CCSGG	2 cut(s)	384, 385
NdeI	CATATG	2 cut(s)	551, 1805
NdeII	GATC	8 cut(s)	322, 337, 868, 958, 1345, 1465, 2286, 2371
NlaIV	GGNNCC	6 cut(s)	324, 382, 870, 1393, 1864, 1960
NmuCI	GTSAC	4 cut(s)	260, 370, 691, 2597
NsiI	ATGCAT	1 cut(s)	1065
NspI	RCATGY	1 cut(s)	2453
OliI	CACNNNNGTG	1 cut(s)	452
PaeR7I	CTCGAG	1 cut(s)	897
PagI	TCATGA	3 cut(s)	1869, 2059, 2337
PciSI	GCTCTTC	1 cut(s)	2548
PfeI	GAWTC	6 cut(s)	7, 601, 916, 1093, 1212, 1439
PfoI	TCCNGGA	1 cut(s)	905
PinAI	ACCGGT	1 cut(s)	1565
PleI	GAGTC	2 cut(s)	367, 2614
PmaCI	CACGTG	1 cut(s)	974
PmlI	CACGTG	1 cut(s)	974
PpsI	GAGTC	2 cut(s)	367, 2614
Ppu21I	YACGTR	2 cut(s)	974, 2602
PsiI	TTATAA	1 cut(s)	2298
Psp6I	CCWGG	6 cut(s)	256, 622, 905, 1194, 1675, 2167
PspCI	CACGTG	1 cut(s)	974
PspFI	CCCAGC	2 cut(s)	2202, 2279
PspGI	CCWGG	6 cut(s)	256, 622, 905, 1194, 1675, 2167
PspN4I	GGNNCC	6 cut(s)	324, 382, 870, 1393, 1864, 1960
PspPI	GGNCC	5 cut(s)	380, 508, 1232, 2119, 2151
PspXI	VCTCGAGB	1 cut(s)	897
PstI	CTGCAG	1 cut(s)	172
PsuI	RGATCY	3 cut(s)	322, 868, 2371
PvuII	CAGCTG	2 cut(s)	299, 2206
RsaI	GTAC	5 cut(s)	255, 398, 596, 1864, 1960
RsaNI	GTAC	5 cut(s)	254, 397, 595, 1863, 1959
RseI	CAYNNNNRTG	5 cut(s)	452, 779, 1284, 1554, 1804
SapI	GCTCTTC	1 cut(s)	2548
SaqAI	TTAA	4 cut(s)	527, 1310, 1578, 1893
Sau3AI	GATC	8 cut(s)	322, 337, 868, 958, 1345, 1465, 2286, 2371
Sau96I	GGNCC	5 cut(s)	380, 508, 1232, 2119, 2151
SchI	GAGTC	2 cut(s)	368, 2615
ScrFI	CCNGG	8 cut(s)	258, 384, 385, 624, 907, 1196, 1677, 2169
SduI	GDGCHC	4 cut(s)	1084, 1512, 1956, 2449
SexAI	ACCWGGT	1 cut(s)	256
SfaNI	GCATC	9 cut(s)	229, 292, 911, 913, 939, 1573, 1618, 1627, 2115
SfcI	CTRYAG	3 cut(s)	168, 939, 1185
Sfr274I	CTCGAG	1 cut(s)	897
SinI	GGWCC	1 cut(s)	2151
SlaI	CTCGAG	1 cut(s)	897
SmaI	CCCGGG	1 cut(s)	385
SmiMI	CAYNNNNRTG	5 cut(s)	452, 779, 1284, 1554, 1804
SmlI	CTYRAG	2 cut(s)	897, 2045
SmoI	CTYRAG	2 cut(s)	897, 2045
SpeI	ACTAGT	1 cut(s)	1709
SsiI	CCGC	4 cut(s)	158, 770, 1448, 2164
SspI	AATATT	2 cut(s)	1986, 2028
SspMI	CTAG	5 cut(s)	251, 1275, 1421, 1710, 2665
StyD4I	CCNGG	8 cut(s)	256, 382, 383, 622, 905, 1194, 1675, 2167
StyI	CCWWGG	2 cut(s)	204, 1274
TaaI	ACNGT	8 cut(s)	682, 829, 880, 1049, 1176, 1286, 1981, 2662
TaiI	ACGT	3 cut(s)	976, 1378, 2604
TaqI	TCGA	9 cut(s)	144, 214, 898, 1442, 1815, 2143, 2256, 2631, 2637
TaqII	GACCGA	1 cut(s)	624
TatI	WGTACW	1 cut(s)	396
TauI	GCSGC	2 cut(s)	1451, 2167
TfiI	GAWTC	6 cut(s)	7, 601, 916, 1093, 1212, 1439
Tru1I	TTAA	4 cut(s)	527, 1310, 1578, 1893
Tru9I	TTAA	4 cut(s)	527, 1310, 1578, 1893
TscAI	CASTG	7 cut(s)	441, 796, 819, 1291, 2232, 2469, 2616
TseFI	GTSAC	4 cut(s)	260, 370, 691, 2597
Tsp45I	GTSAC	4 cut(s)	260, 370, 691, 2597
TspDTI	ATGAA	8 cut(s)	130, 1127, 1398, 1452, 1748, 1777, 1819, 2326
TspGWI	ACGGA	2 cut(s)	679, 1315
TspMI	CCCGGG	1 cut(s)	383
TspRI	CASTG	7 cut(s)	441, 796, 819, 1291, 2232, 2469, 2616
VneI	GTGCAC	1 cut(s)	1080
VpaK11BI	GGWCC	1 cut(s)	2151
XapI	RAATTY	2 cut(s)	541, 660
XceI	RCATGY	1 cut(s)	2453
XhoI	CTCGAG	1 cut(s)	897
XmaI	CCCGGG	1 cut(s)	383
XmaJI	CCTAGG	1 cut(s)	1274
XspI	CTAG	5 cut(s)	251, 1275, 1421, 1710, 2665
ZraI	GACGTC	1 cut(s)	1376
Zsp2I	ATGCAT	1 cut(s)	1065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.