MD17G1046600.v1.1

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002286
Physical Location & Seq
Forward (+)
3427186 .. 3433399
6214 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1046600.v1.1.491

Sequence Viewer

Length: 2871 bp
ATGGATGAGAAAGCTGTCGATTTGGACGCCGTGTTGAAGGAAGCTGTCGATTTGGAACACGTGCCACTTGAAGAAGTTTTTGTGACCTTGAGGTGCAACAAACATGGGCTCACATCTGAGGCTGTTGAGCAGAGGTTGGCCATTTTTGGGTTCAACAAGCTTGAGGAGAAGAAGGAAAGTAAGGTTTTGAAGTTTCTAGGGTTTATGTGGAACCCTCTCGCATGGGTGATGGAAGCTGCAGCTATCATGGCCATAGCTCTTGCCAATGGAGGAGGAAAGCCACCTGATTGGCAAGATTTTATTGGCATCATTGCTCTGCTGATAATCAACTCAACTATAAGTTTCATAGAAGAGAATAACGCGGGAAATGCCGCTGCTGCTCTCATGGCTCGTCTCGCCCCCAAAGCCAAGGTTTTTCGAGATGGAAGGTGGATCGAGGAGGATGCTTCCGTTCTTGTCCCCGGTGATGTAATCAGTATTAAGCTTGGGGACATTATTCCAGCTGATGCTCGTCTACTTGACGGCGATGCACTGAAAATTGATCAGTCGGCGCTTACAGGCGAGTCACTTCCGGTGACGAAAGGCCCTGGCAGCAGTGTGTATTCAGGTTCTACTTGCAAACAGGGAGAGATTGAAGCAGTGGTAATTGCCACAGGTGTTCATACCTTTTTCGGCAAGGCAGCTCATCTTGTGGATAGCACAAATCAACAAGGCCACTTTCAAAAGGTCTTGACTGCCATTGGGAATTTCTGTATATGTTCGATTGCGGTGGGAATGATTGTCGAGATCATAGTTATGTATCCAATTCAACACCGCGAATATCGCCCAGGAATCGACAATCTGCTGGTGCTACTCATCGGAGGAATTCCCATTGCTATGCCCACAGTTTTATCTGTCACAATGGCTATTGGTTCCCATCGCTTATCTCAGCAGGGCGCTATCACTAAAAGAATGACGGCAATTGAGGAGATGGCAGGCATGGATGTCCTTTGCAGTGACAAAACCGGAACTCTGACGTTGAATAAGCTTTCGGTTGACAAGAATCTCGTAGAGATCTTCGCTAAAGAGGTGGACGCAGACACTGTTGTTCTAATGGCAGCTCGAGCCTCCCGAATGGAAAATCAAGACGCAATAGATGCTGCTATTGTTGGAATGTTGGCTGATCCAAAAGAGGCCAGAGCCGGTATCCAAGAGGTGCACTTCCTTCCATTCAACCCGACAGATAAGCGAACAGCTCTGACATACATTGACAGTCAGGGTAAAATGCATCGTGTCAGCAAGGGTGCACCGGAGCAGATTTTGAACCTTGTACGCAATAAATCAGAGATAGAACGTAGAGTTCACACCGTGATTGATAAATTCGCAGAGCGAGGTTTACGATCCTTAGCAGTAGCATACCAGGAAGTTCCAGATGGAAGGAAAGAGAGCCAGGGAGGGCCTTGGCAATTTATCGGCCTCTTGCCTCTGTTCGACCCACCTAGGCATGATAGCGCAGAGACGATTAGAAGGGCATTGAATCTTGGGGTAAATGTTAAGATGATTACAGGTGATCAACTGGCAATAGCGAAGGAGACAGGGCGCCGCCTGGGCATGGGAACCAACATGTATCCATCATCCAGTCTGTTAGGACAGAAGAAAGATGAGTCGATTGTCGCTCTGCCAGTTGATGAACTCATTGAAAAAGCTGATGGTTTTGCTGGTGTATTCCCTGAGCACAAGTACGAAATTGTGAAGCGATTGCAAGCCAGAAAGCATATTTGCGGAATGACTGGTGACGGCGTTAATGATGCTCCTGCTCTTAAAAAGGCTGATATCGGCATTGCTGTTGCTGATGCAACTGACGCAGCTCGTAGTGCTTCGGACATTGTCCTCACCGAACCTGGCCTTAGCGTCATCATCAGCGCCGTGCTAACTAGTCGGTCCATATTCCAGAGAATGAAAAATTACACGATATATGCTGTTTCCATTACAATCCGTATCGTGCTTGGTTTCATGTTACTGGCACTCATATGGCAATTCGACTTCCCGCCCTTCATGGTGCTGATTATTGCTATTCTCAATGACGGTACCATCATGACGATATCAAAGGATAGAGTGAAACCATCTCCGCAGCCGGACAGCTGGAAACTGGCGGAGATCTTTACGACTGGCATCGTCCTCGGTGGTTACTTGGCTATGATGACAGTCATTTTCTTTTGGGCAGCATACGATACAGACTTCTTTCCTCGTACCTGTGGGGTTTCGAGCCTTCACCATAAGGACGTGGATGACTACAGGAAGCTCGCCGCTGCAGTTTACCTGCAAGTGAGTACCATCAGCCAGGCCCTTATATTCGTCACACGAGCACGGAGTTGGTCTTTTGTCGAGCGTCCTGGTCTGCTGCTTGTAGCGGCTTTTGTCATTGCTCAGTTGATTGCTACGCTGATCGCTGTCTACGCCAGTTGGAGTTTTGCTGCGATTGAGGGAATCGGATGGGGGTGGGCTGGCGTGGTTTGGCTGTATAACCTCATTTTCTACTTCCCGCTCGATCTCATCAAGTTCTTCATCCGATATGCCCTGAGCGGGCGAGCTTGGGATCTCGTAATTGAGCAAAGGACTGCCTTCACAAGGAAGAAGGATTTCGGGAAGGAAGAAAGGGCGCTTAAATGGGCACATGCACAGAGAACCCTCCATGGACTGCACCCACCGGAGGCCACGATGTTCAGCGATCGCACTACTTATACAGAACTCAATCAGATGGCTGAAGAGGCAAAACGACGGGCTGAAATTGCCAGGCTGAGGGAATTGCAGACCCTTAAAGGGCGTGTGGAATCGGTAGTGAGGCTGAAGAACCTGGACATAGACACAATCCAGCAAGCATACACTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

957

Amino Acids

105.31

Weight (kDa)

6.45

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 20 - 83 4e-14 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 132 - 231 5.6e-30 P-type ATPase actuator domain
Hydrolase PF00702 327 - 604 1.3e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 376 - 495 1.5e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2307
Acc65I GGTACC 1 cut(s) 2066
AccB1I GGYRCC 2 cut(s) 1578, 2066
AccBSI CCGCTC 2 cut(s) 2524, 2562
AccI GTMKAC 2 cut(s) 514, 2433
AccII CGCG 2 cut(s) 362, 816
AclWI GGATC 4 cut(s) 440, 1157, 1374, 2583
AcoI YGGCCR 2 cut(s) 138, 249
AcsI RAATTY 3 cut(s) 745, 864, 1358
AcuI CTGAAG 2 cut(s) 2763, 2846
AcvI CACGTG 1 cut(s) 61
AcyI GRCGYC 2 cut(s) 27, 1579
AdeI CACNNNGTG 1 cut(s) 1348
AfaI GTAC 5 cut(s) 1311, 1721, 2068, 2230, 2311
AfiI CCNNNNNNNGG 7 cut(s) 147, 1591, 2562, 2607, 2689, 2778, 2799
AflIII ACRYGT 2 cut(s) 58, 1602
AhlI ACTAGT 1 cut(s) 1913
AjiI CACGTC 1 cut(s) 2263
Alw21I GWGCWC 4 cut(s) 1200, 1288, 1716, 2347
Alw26I GTCTC 3 cut(s) 398, 1490, 1565
Alw44I GTGCAC 2 cut(s) 1196, 1284
AlwI GGATC 4 cut(s) 440, 1157, 1374, 2583
AlwNI CAGNNNCTG 1 cut(s) 1082
Ama87I CYCGRG 1 cut(s) 1101
ApaLI GTGCAC 2 cut(s) 1196, 1284
ApoI RAATTY 3 cut(s) 745, 864, 1358
AsiSI GCGATCGC 1 cut(s) 2710
Asp700I GAANNNNTTC 1 cut(s) 2618
Asp718I GGTACC 1 cut(s) 2066
AspA2I CCTAGG 1 cut(s) 1478
AspLEI GCGC 6 cut(s) 553, 938, 1493, 1581, 1904, 2641
AspS9I GGNCC 4 cut(s) 584, 1436, 1920, 2323
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 7 cut(s) 238, 476, 586, 1559, 1784, 1864, 2243
AvaI CYCGRG 1 cut(s) 1101
AvaII GGWCC 1 cut(s) 1920
AvrII CCTAGG 1 cut(s) 1478
BaeGI GKGCMC 3 cut(s) 1200, 1288, 2653
BalI TGGCCA 2 cut(s) 140, 251
BanI GGYRCC 2 cut(s) 1578, 2066
BanII GRGCYC 1 cut(s) 111
BauI CACGAG 1 cut(s) 2340
BbrPI CACGTG 1 cut(s) 61
Bbv12I GWGCWC 4 cut(s) 1200, 1288, 1716, 2347
BbvCI CCTCAGC 1 cut(s) 2777
BceAI ACGGC 5 cut(s) 14, 538, 972, 1792, 1889
BcgI CGANNNNNNTGC 4 cut(s) 425, 459, 2140, 2174
BciVI GTATCC 3 cut(s) 810, 1196, 1617
BclI TGATCA 2 cut(s) 541, 1549
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 3 cut(s) 398, 1490, 1565
BcuI ACTAGT 1 cut(s) 1913
BfaI CTAG 3 cut(s) 197, 1479, 1914
BfmI CTRYAG 3 cut(s) 237, 2272, 2289
BfoI RGCGCY 5 cut(s) 554, 939, 1582, 1905, 2642
BfuAI ACCTGC 1 cut(s) 2307
BfuI GTATCC 3 cut(s) 810, 1196, 1617
BglI GCCNNNNNGGC 1 cut(s) 1587
BglII AGATCT 2 cut(s) 1053, 2136
BlnI CCTAGG 1 cut(s) 1478
Bme18I GGWCC 1 cut(s) 1920
BmeT110I CYCGRG 1 cut(s) 1101
BmgBI CACGTC 1 cut(s) 2263
BmgT120I GGNCC 4 cut(s) 584, 1436, 1920, 2323
BmiI GGNNCC 5 cut(s) 212, 913, 1580, 1597, 2068
BmsI GCATC 9 cut(s) 315, 433, 496, 517, 1126, 1276, 1777, 1822, 2160
Bpu10I CCTNAGC 5 cut(s) 1384, 1710, 1886, 2558, 2777
BpuEI CTTGAG 2 cut(s) 109, 182
BpuMI CCSGG 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 61
BsaHI GRCGYC 2 cut(s) 27, 1579
BsaWI WCCGGW 4 cut(s) 571, 1004, 1288, 2686
Bsc4I CCNNNNNNNGG 7 cut(s) 147, 1591, 2562, 2607, 2689, 2778, 2799
Bse118I RCCGGY 1 cut(s) 1181
Bse1I ACTGG 8 cut(s) 1560, 1617, 1661, 1774, 2004, 2133, 2152, 2439
Bse3DI GCAATG 4 cut(s) 309, 870, 1818, 2400
BseGI GGATG 7 cut(s) 10, 448, 988, 1613, 2272, 2477, 2544
BseLI CCNNNNNNNGG 7 cut(s) 147, 1591, 2562, 2607, 2689, 2778, 2799
BseMI GCAATG 4 cut(s) 309, 870, 1818, 2400
BseMII CTCAG 6 cut(s) 108, 941, 1701, 2420, 2549, 2768
BseNI ACTGG 8 cut(s) 1560, 1617, 1661, 1774, 2004, 2133, 2152, 2439
BseRI GAGGAG 4 cut(s) 179, 285, 452, 980
BseSI GKGCMC 3 cut(s) 1200, 1288, 2653
BsgI GTGCAG 1 cut(s) 2663
Bsh1236I CGCG 2 cut(s) 362, 816
Bsh1285I CGRYCG 1 cut(s) 2710
BshNI GGYRCC 2 cut(s) 1578, 2066
BsiEI CGRYCG 1 cut(s) 2710
BsiHKAI GWGCWC 4 cut(s) 1200, 1288, 1716, 2347
BsiHKCI CYCGRG 1 cut(s) 1101
BsiSI CCGG 7 cut(s) 462, 572, 1005, 1182, 1289, 2114, 2687
BslFI GGGAC 2 cut(s) 443, 503
BslI CCNNNNNNNGG 7 cut(s) 147, 1591, 2562, 2607, 2689, 2778, 2799
BsmAI GTCTC 3 cut(s) 398, 1490, 1565
BsmBI CGTCTC 2 cut(s) 398, 1490
BsmFI GGGAC 2 cut(s) 443, 503
BsoBI CYCGRG 1 cut(s) 1101
Bsp1286I GDGCHC 6 cut(s) 111, 1200, 1288, 1716, 2347, 2653
Bsp19I CCATGG 1 cut(s) 2671
BspCNI CTCAG 6 cut(s) 109, 940, 1702, 2419, 2550, 2769
BspFNI CGCG 2 cut(s) 362, 816
BspHI TCATGA 1 cut(s) 2073
BspLI GGNNCC 5 cut(s) 212, 913, 1580, 1597, 2068
BspMAI CTGCAG 2 cut(s) 241, 2293
BspMI ACCTGC 1 cut(s) 2307
BspPI GGATC 4 cut(s) 440, 1157, 1374, 2583
BspT107I GGYRCC 2 cut(s) 1578, 2066
BsrBI CCGCTC 2 cut(s) 2524, 2562
BsrDI GCAATG 4 cut(s) 309, 870, 1818, 2400
BsrFI RCCGGY 1 cut(s) 1181
BsrI ACTGG 8 cut(s) 1560, 1617, 1661, 1774, 2004, 2133, 2152, 2439
BssAI RCCGGY 1 cut(s) 1181
BssNI GRCGYC 2 cut(s) 27, 1579
BssSI CACGAG 1 cut(s) 2340
BssT1I CCWWGG 4 cut(s) 408, 1439, 1478, 2671
Bst2BI CACGAG 1 cut(s) 2340
Bst4CI ACNGT 7 cut(s) 886, 1084, 1253, 1348, 2066, 2185, 2866
Bst6I CTCTTC 2 cut(s) 345, 2739
BstACI GRCGYC 2 cut(s) 27, 1579
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstBAI YACGTR 1 cut(s) 61
BstC8I GCNNGC 7 cut(s) 976, 1743, 2283, 2485, 2564, 2568, 2856
BstDEI CTNAG 8 cut(s) 117, 927, 1384, 1710, 1886, 2406, 2558, 2777
BstDSI CCRYGG 1 cut(s) 2671
BstENI CCTNNNNNAGG 1 cut(s) 2605
BstF5I GGATG 7 cut(s) 10, 448, 988, 1613, 2272, 2477, 2544
BstFNI CGCG 2 cut(s) 362, 816
BstH2I RGCGCY 5 cut(s) 554, 939, 1582, 1905, 2642
BstHHI GCGC 6 cut(s) 553, 938, 1493, 1581, 1904, 2641
BstMAI GTCTC 3 cut(s) 398, 1490, 1565
BstMCI CGRYCG 1 cut(s) 2710
BstNSI RCATGY 2 cut(s) 1606, 2657
BstSFI CTRYAG 3 cut(s) 237, 2272, 2289
BstSLI GKGCMC 3 cut(s) 1200, 1288, 2653
BstUI CGCG 2 cut(s) 362, 816
BstX2I RGATCY 3 cut(s) 1053, 2136, 2575
BstXI CCANNNNNNTGG 1 cut(s) 288
BstYI RGATCY 3 cut(s) 1053, 2136, 2575
BsuI GTATCC 3 cut(s) 810, 1196, 1617
BtgI CCRYGG 1 cut(s) 2671
BtgZI GCGATG 2 cut(s) 540, 902
BtrI CACGTC 1 cut(s) 2263
BtsCI GGATG 7 cut(s) 10, 448, 988, 1613, 2272, 2477, 2544
BtsI GCAGTG 3 cut(s) 601, 645, 1000
BtsIMutI CAGTG 6 cut(s) 530, 601, 645, 1000, 1080, 2862
BveI ACCTGC 1 cut(s) 2307
Cac8I GCNNGC 7 cut(s) 976, 1743, 2283, 2485, 2564, 2568, 2856
CaiI CAGNNNCTG 1 cut(s) 1082
CciI TCATGA 1 cut(s) 2073
CfoI GCGC 6 cut(s) 553, 938, 1493, 1581, 1904, 2641
Cfr10I RCCGGY 1 cut(s) 1181
Cfr13I GGNCC 4 cut(s) 584, 1436, 1920, 2323
CseI GACGC 6 cut(s) 35, 1082, 1136, 1850, 1879, 2357
Csp6I GTAC 5 cut(s) 1310, 1720, 2067, 2229, 2310
CviQI GTAC 5 cut(s) 1310, 1720, 2067, 2229, 2310
DdeI CTNAG 8 cut(s) 117, 927, 1384, 1710, 1886, 2406, 2558, 2777
DinI GGCGCC 1 cut(s) 1580
DraIII CACNNNGTG 1 cut(s) 1348
EaeI YGGCCR 2 cut(s) 138, 249
Eam1104I CTCTTC 2 cut(s) 345, 2739
EarI CTCTTC 2 cut(s) 345, 2739
EciI GGCGGA 1 cut(s) 2147
Eco130I CCWWGG 4 cut(s) 408, 1439, 1478, 2671
Eco24I GRGCYC 1 cut(s) 111
Eco32I GATATC 2 cut(s) 1813, 2082
Eco47I GGWCC 1 cut(s) 1920
Eco57I CTGAAG 2 cut(s) 2763, 2846
Eco72I CACGTG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 1101
EcoNI CCTNNNNNAGG 1 cut(s) 2605
EcoO109I RGGNCCY 3 cut(s) 584, 1436, 2323
EcoRI GAATTC 1 cut(s) 864
EcoRV GATATC 2 cut(s) 1813, 2082
EcoT14I CCWWGG 4 cut(s) 408, 1439, 1478, 2671
EcoT22I ATGCAT 1 cut(s) 1269
EcoT38I GRGCYC 1 cut(s) 111
EgeI GGCGCC 1 cut(s) 1580
EheI GGCGCC 1 cut(s) 1580
ErhI CCWWGG 4 cut(s) 408, 1439, 1478, 2671
Esp3I CGTCTC 2 cut(s) 398, 1490
FaqI GGGAC 2 cut(s) 443, 503
FauI CCCGC 4 cut(s) 355, 2034, 2529, 2555
FauNDI CATATG 1 cut(s) 2009
FbaI TGATCA 2 cut(s) 541, 1549
FblI GTMKAC 2 cut(s) 514, 2433
FokI GGATG 7 cut(s) 17, 455, 995, 1600, 2279, 2484, 2531
FriOI GRGCYC 1 cut(s) 111
FspBI CTAG 3 cut(s) 197, 1479, 1914
GlaI GCGC 6 cut(s) 552, 937, 1492, 1580, 1903, 2640
HaeII RGCGCY 5 cut(s) 554, 939, 1582, 1905, 2642
HapII CCGG 7 cut(s) 462, 572, 1005, 1182, 1289, 2114, 2687
HgaI GACGC 6 cut(s) 35, 1082, 1136, 1850, 1879, 2357
HhaI GCGC 6 cut(s) 553, 938, 1493, 1581, 1904, 2641
Hin1I GRCGYC 2 cut(s) 27, 1579
Hin6I GCGC 6 cut(s) 551, 936, 1491, 1579, 1902, 2639
HinP1I GCGC 6 cut(s) 551, 936, 1491, 1579, 1902, 2639
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HindIII AAGCTT 3 cut(s) 158, 482, 1025
HinfI GANTC 7 cut(s) 563, 831, 1042, 1516, 1643, 2466, 2810
HpaII CCGG 7 cut(s) 462, 572, 1005, 1182, 1289, 2114, 2687
HphI GGTGA 7 cut(s) 238, 476, 586, 1559, 1784, 1864, 2243
Hpy166II GTNNAC 9 cut(s) 515, 1036, 1072, 1198, 1286, 1342, 1376, 2296, 2434
Hpy188I TCNGA 9 cut(s) 118, 860, 1014, 1239, 1324, 1861, 2471, 2549, 2736
Hpy188III TCNNGA 9 cut(s) 419, 730, 784, 1110, 1124, 1409, 1930, 2074, 2623
Hpy8I GTNNAC 9 cut(s) 515, 1036, 1072, 1198, 1286, 1342, 1376, 2296, 2434
Hpy99I CGWCG 1 cut(s) 2760
HpyCH4III ACNGT 7 cut(s) 886, 1084, 1253, 1348, 2066, 2185, 2866
HpyCH4IV ACGT 4 cut(s) 60, 1016, 1333, 2262
HpyF3I CTNAG 8 cut(s) 117, 927, 1384, 1710, 1886, 2406, 2558, 2777
HpySE526I ACGT 4 cut(s) 60, 1016, 1333, 2262
Hsp92I GRCGYC 2 cut(s) 27, 1579
HspAI GCGC 6 cut(s) 551, 936, 1491, 1579, 1902, 2639
KasI GGCGCC 1 cut(s) 1578
KpnI GGTACC 1 cut(s) 2070
Ksp22I TGATCA 2 cut(s) 541, 1549
LmnI GCTCC 2 cut(s) 1291, 1795
LweI GCATC 9 cut(s) 315, 433, 496, 517, 1126, 1276, 1777, 1822, 2160
MaeI CTAG 3 cut(s) 197, 1479, 1914
MaeII ACGT 4 cut(s) 60, 1016, 1333, 2262
MaeIII GTNAC 9 cut(s) 82, 564, 574, 895, 995, 1772, 1995, 2165, 2335
MbiI CCGCTC 2 cut(s) 2524, 2562
MfeI CAATTG 1 cut(s) 960
MflI RGATCY 3 cut(s) 1053, 2136, 2575
MhlI GDGCHC 6 cut(s) 111, 1200, 1288, 1716, 2347, 2653
MlsI TGGCCA 2 cut(s) 140, 251
MluNI TGGCCA 2 cut(s) 140, 251
Mly113I GGCGCC 1 cut(s) 1579
MlyI GAGTC 2 cut(s) 572, 1652
MmeI TCCRAC 2 cut(s) 1129, 2423
Mox20I TGGCCA 2 cut(s) 140, 251
Mph1103I ATGCAT 1 cut(s) 1269
MroXI GAANNNNTTC 1 cut(s) 2618
MscI TGGCCA 2 cut(s) 140, 251
MseI TTAA 6 cut(s) 480, 1533, 1782, 1800, 2643, 2796
MslI CAYNNNNRTG 3 cut(s) 794, 875, 2008
Msp20I TGGCCA 2 cut(s) 140, 251
MspA1I CMGCKG 4 cut(s) 374, 503, 2121, 2288
MspI CCGG 7 cut(s) 462, 572, 1005, 1182, 1289, 2114, 2687
MunI CAATTG 1 cut(s) 960
MvnI CGCG 2 cut(s) 362, 816
NarI GGCGCC 1 cut(s) 1579
NciI CCSGG 1 cut(s) 462
NcoI CCATGG 1 cut(s) 2671
NdeI CATATG 1 cut(s) 2009
NlaIV GGNNCC 5 cut(s) 212, 913, 1580, 1597, 2068
NmuCI GTSAC 7 cut(s) 82, 564, 574, 895, 995, 1772, 2335
NsiI ATGCAT 1 cut(s) 1269
NspI RCATGY 2 cut(s) 1606, 2657
PaeR7I CTCGAG 1 cut(s) 1101
PagI TCATGA 1 cut(s) 2073
PciI ACATGT 1 cut(s) 1602
PcsI WCGNNNNNNNCGW 1 cut(s) 1108
PdmI GAANNNNTTC 1 cut(s) 2618
PfeI GAWTC 5 cut(s) 831, 1042, 1516, 2466, 2810
PflFI GACNNNGTC 1 cut(s) 1865
Ple19I CGATCG 1 cut(s) 2710
PleI GAGTC 2 cut(s) 571, 1651
PluTI GGCGCC 1 cut(s) 1582
PmaCI CACGTG 1 cut(s) 61
PmlI CACGTG 1 cut(s) 61
PpsI GAGTC 2 cut(s) 571, 1651
Ppu21I YACGTR 1 cut(s) 61
PscI ACATGT 1 cut(s) 1602
PspCI CACGTG 1 cut(s) 61
PspN4I GGNNCC 5 cut(s) 212, 913, 1580, 1597, 2068
PspPI GGNCC 4 cut(s) 584, 1436, 1920, 2323
PspXI VCTCGAGB 1 cut(s) 1101
PstI CTGCAG 2 cut(s) 241, 2293
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 3 cut(s) 1053, 2136, 2575
PsyI GACNNNGTC 1 cut(s) 1865
PvuI CGATCG 1 cut(s) 2710
PvuII CAGCTG 2 cut(s) 503, 2121
RgaI GCGATCGC 1 cut(s) 2710
RsaI GTAC 5 cut(s) 1311, 1721, 2068, 2230, 2311
RsaNI GTAC 5 cut(s) 1310, 1720, 2067, 2229, 2310
RseI CAYNNNNRTG 3 cut(s) 794, 875, 2008
SaqAI TTAA 6 cut(s) 480, 1533, 1782, 1800, 2643, 2796
Sau96I GGNCC 4 cut(s) 584, 1436, 1920, 2323
SchI GAGTC 2 cut(s) 572, 1652
SduI GDGCHC 6 cut(s) 111, 1200, 1288, 1716, 2347, 2653
SfaAI GCGATCGC 1 cut(s) 2710
SfaNI GCATC 9 cut(s) 315, 433, 496, 517, 1126, 1276, 1777, 1822, 2160
SfcI CTRYAG 3 cut(s) 237, 2272, 2289
SfoI GGCGCC 1 cut(s) 1580
Sfr274I CTCGAG 1 cut(s) 1101
SgfI GCGATCGC 1 cut(s) 2710
SinI GGWCC 1 cut(s) 1920
SlaI CTCGAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 3 cut(s) 794, 875, 2008
SmlI CTYRAG 3 cut(s) 88, 161, 1101
SmoI CTYRAG 3 cut(s) 88, 161, 1101
SpeI ACTAGT 1 cut(s) 1913
SspDI GGCGCC 1 cut(s) 1578
SspMI CTAG 3 cut(s) 197, 1479, 1914
StyI CCWWGG 4 cut(s) 408, 1439, 1478, 2671
TaaI ACNGT 7 cut(s) 886, 1084, 1253, 1348, 2066, 2185, 2866
TaiI ACGT 4 cut(s) 63, 1019, 1336, 2265
TaqII GACCGA 1 cut(s) 1908
TauI GCSGC 4 cut(s) 374, 1584, 2288, 2393
TfiI GAWTC 5 cut(s) 831, 1042, 1516, 2466, 2810
Tru1I TTAA 6 cut(s) 480, 1533, 1782, 1800, 2643, 2796
Tru9I TTAA 6 cut(s) 480, 1533, 1782, 1800, 2643, 2796
TscAI CASTG 6 cut(s) 537, 601, 645, 1000, 1087, 2869
TseFI GTSAC 7 cut(s) 82, 564, 574, 895, 995, 1772, 2335
Tsp45I GTSAC 7 cut(s) 82, 564, 574, 895, 995, 1772, 2335
TspDTI ATGAA 7 cut(s) 334, 650, 1683, 1952, 1981, 2023, 2533
TspGWI ACGGA 3 cut(s) 439, 1964, 2362
TspRI CASTG 6 cut(s) 537, 601, 645, 1000, 1087, 2869
Tth111I GACNNNGTC 1 cut(s) 1865
VneI GTGCAC 2 cut(s) 1196, 1284
VpaK11BI GGWCC 1 cut(s) 1920
XagI CCTNNNNNAGG 1 cut(s) 2605
XapI RAATTY 3 cut(s) 745, 864, 1358
XceI RCATGY 2 cut(s) 1606, 2657
XhoI CTCGAG 1 cut(s) 1101
XmaJI CCTAGG 1 cut(s) 1478
XmiI GTMKAC 2 cut(s) 514, 2433
XmnI GAANNNNTTC 1 cut(s) 2618
XspI CTAG 3 cut(s) 197, 1479, 1914
Zsp2I ATGCAT 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.