MD14G1192600.v1.1

ATPase 11, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002286
Physical Location & Seq
Forward (+)
28352453 .. 28358745
6293 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1192600.v1.1.491

Sequence Viewer

Length: 2871 bp
ATGGGGGAGAAACCTGAAGTATTGGATGCTGTGTTGAAGGAAACTGTTGATTTGGAAAATATACCCATTGAAGAGGTATTTGAGAATCTGAGATGTAGCAAAGAGGGTCTCAGCAGTGAAGCTGCTGAGGAAAGACTCACCATTTTTGGACACAACAAGCTTGAAGAAAAGCAGGAGAGCAAATTTTTGAAGTTTTTGGGTTTTATGTGGAATCCTCTCTCATGGGTTATGGAAGCTGCTGCCATAATGGCCATTGCTCTTGCAAATGGAGGGGGTAAGCCACCGGATTGGCAAGATTTTGTGGGTATTATGACTCTGCTCGTCATCAATTCCACTATCAGTTTCATTGAGGAAAACAATGCCGGTAATGCTGCAGCAGCACTCATGGCTCGTTTGGCTCCCAAAGCCAAGGTCCTTCGAGATGGAAGGTGGAATGAACAAGATGCTTCGGTTCTAGTTCCTGGTGATATAATTAGTATTAAGCTTGGTGACATTGTTCCAGCTGATGCTCGTCTACTTGAAGGGGATCCTTTGAAAATTGACCAGTCTGCACTTACAGGTGAATCCCTTGCCGTGACGAAAAGCCCTGGGGATGGTGTGTATTCTGGTTCTACTTGCAAACAAGGAGAGATTGATGCAGTGGTCATTGCCACCGGTGTCCATACCTTCTTTGGAAAAGCTGCTCACCTTGTTGATAGCACAAATCAAGTGGGCCATTTCCAAAAGGTCTTGACTGCAATTGGGAATTTCTGCATATGTTCCATTGCTGTGGGGATGGTAATAGAGATAATTGTCATGTACCCGATTCAAGACCGAAAATATCGTCCTGGAATTGATAACCTTCTTGTGCTTCTCATTGGTGGAATTCCAATTGCCATGCCGACAGTTCTGTCTGTGACAATGGCTATTGGGTCTCATAGATTATCACAGCAGGGAGCTATTACAAAGAGAATGACAGCAATTGAAGAGATGGCAGGCATGGATGTTCTTTGCAGTGATAAGACTGGAACTCTGACATTGAATAAGCTTACTGTTGACAAAAATCTTATCGAGGTTTTTGCAAAAGGAGTGGATCCAGATACTGTTGTGTTGATGGCAGCTCGAGCCTCCAGACTGGAGAATCAAGATGCAATAGATACTGCTATAGTTGGGATGCTTGCTGATCCTAAGGAGGCACGTGCTGGAATTCAAGAAATACACTTCCTTCCTTTCAATCCTACTGATAAAAGAACTGCATTGACTTATCTAGATAGTGATGGTAAAATGCATAGAGTTAGCAAAGGTGCACCAGAGCAGATTTTGAATCTTGCACGCAATAAGTCAGACATCGAGCGCCGAGTTCATGCTGTTATTGATAAGTTTGCTGAGCGAGGTTTACGATCTCTTGCTGTAGCATACCAGGAAGTTCCAGAAGGAAGGAAGGAGAGTGCTGGAGCCCCGTGGCAATTTGTTGGTGTAATGCCACTCTTTGACCCACCTAGACATGACAGTGCAGAGACAATAAGGAGGGCGTTAAATCTTGGAGTGAATGTGAAGATGATTACTGGGGATCAACTGGCGATTGGAAAGGAAACTGGACGTCGCTTGGGGATGGGAACCAATATGTACCCTTCATCTGCTTTGCTAGGACAGGACAAGGATGAGTCGATTATTGCTTTACCGATTGACGAGCTGATAGAAAAAGCTGATGGCTTTGCTGGGGTTTTCCCAGAGCACAAATACGAGATTGTGAAACGCTTGCAAGCTAGGAAACATATCTGTGGAATGACTGGTGATGGAGTCAATGATGCTCCTGCCCTCAAAAAAGCTGATATTGGTATAGCTGTTGCTGATGCAACAGATGCTGCTCGCAGTGCTTCTGATATTGTGCTTACTGAACCTGGCCTTAGTGTCATCATTAGTGCTGTTTTGACCAGTCGGGCAATCTTCCAGAGGATGAAAAATTACACAATATATGCTGTTTCCATCACAATTCGTATTGTGCTTGGTTTCATGTTGCTGGCTCTCCTATGGAAGTTTGACTTTCCGCCTTTCATGGTGCTTATTATTGCGATCCTCAATGATGGTACGATCATGACAATATCAAAGGACAGAGTTAAACCGTCTCCTCTGCCAGATAGCTGGAAGCTGGCCGAGATTTTTGCAACTGGAATTGTGCTCGGAGGCTACTTGGCAATAATGACTGTGATATTCTTTTGGGCAGCATACAAAACAGACTTCTTCCCAAGAACATTTGGGGTATCAACCCTTGAGAAAACAGCTAATGATGACTTTCGGAAGCTTGCCTCTGCTATATATCTACAAGTGAGCATTATTAGCCAGGCCCTCATATTTGTTACACGGTCTCGAAGTTGGTCGTTTATTGAGCGACCTGGATTGTTGCTTATGGTGGCTTTTATAATAGCTCAGCTGATAGCCACCTTGATTGCAGTCTATGCAAATTGGAGTTTTGCTGCCATTGAGGGGATCGGTTGGGGTTGGGCCGGGGTGATATGGCTTTATAATATCGTCACATATATCCCTCTGGATATCATAAAATTCATGATACGTTATGCTCTGAGCGGGAAGGCGTGGGATCTACTGATTGAACAGAGGGTTGCTTTCACAAGGCAAAAGGACTTTGGAAAGGAACAACGTGAGCTTCAATGGGCACACGCACAAAGGACATTGCACGGGTTGCAGCCACCGGACACCAAGATGTTTACAGAACGAACACATTTCACAGAACTCAATCAGATCGCCGAAGAGGCCAAAAGAAGAGCTGAGATTGCAAGGTTGAGGGAACTCACTACACTGAAGGGTCACGTAGAATCCGTGGTGAGATTGAAGGGGCTCGACATCGACACAATTCAGCAATCATACACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

957

Amino Acids

105.18

Weight (kDa)

6.1

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 20 - 83 1.8e-15 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 132 - 231 7.4e-30 P-type ATPase actuator domain
Hydrolase PF00702 327 - 604 1.9e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 375 - 495 9.3e-07 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2399, 2502
AasI GACNNNNNNGTC 1 cut(s) 889
AatII GACGTC 1 cut(s) 1582
AccBSI CCGCTC 1 cut(s) 2562
AccI GTMKAC 1 cut(s) 514
AciI CCGC 2 cut(s) 2027, 2562
AclWI GGATC 9 cut(s) 521, 534, 1067, 1080, 1157, 1557, 2047, 2474, 2583
AcoI YGGCCR 2 cut(s) 249, 2130
AcsI RAATTY 5 cut(s) 182, 745, 864, 1185, 2537
AcuI CTGAAG 2 cut(s) 36, 2816
AcvI CACGTG 1 cut(s) 1178
AcyI GRCGYC 1 cut(s) 1579
AfaI GTAC 3 cut(s) 800, 1607, 2068
AfiI CCNNNNNNNGG 2 cut(s) 593, 2463
AgeI ACCGGT 1 cut(s) 653
AjnI CCWGG 7 cut(s) 460, 586, 826, 1398, 1879, 2319, 2371
Alw21I GWGCWC 3 cut(s) 1288, 1716, 2160
Alw26I GTCTC 5 cut(s) 113, 918, 1490, 2109, 2349
Alw44I GTGCAC 1 cut(s) 1284
AlwI GGATC 9 cut(s) 521, 534, 1067, 1080, 1157, 1557, 2047, 2474, 2583
AlwNI CAGNNNCTG 2 cut(s) 1082, 1844
Ama87I CYCGRG 1 cut(s) 1101
AoxI GGCC 7 cut(s) 249, 712, 1882, 2130, 2322, 2481, 2748
ApaLI GTGCAC 1 cut(s) 1284
ApoI RAATTY 5 cut(s) 182, 745, 864, 1185, 2537
ArsI GACNNNNNNTTYG 4 cut(s) 396, 428, 808, 840
AsiGI ACCGGT 1 cut(s) 653
AspLEI GCGC 1 cut(s) 1335
AspS9I GGNCC 4 cut(s) 412, 712, 2323, 2481
AsuC2I CCSGG 1 cut(s) 2485
AsuHPI GGTGA 8 cut(s) 130, 476, 500, 572, 677, 1784, 2500, 2830
AvaI CYCGRG 1 cut(s) 1101
AvaII GGWCC 1 cut(s) 412
AxyI CCTNAGG 1 cut(s) 1167
BaeGI GKGCMC 2 cut(s) 1288, 2653
BaeI ACNNNNGTAYC 2 cut(s) 1071, 1104
BalI TGGCCA 1 cut(s) 251
BamHI GGATCC 2 cut(s) 526, 1072
BanII GRGCYC 2 cut(s) 1438, 2835
BbrPI CACGTG 1 cut(s) 1178
Bbv12I GWGCWC 3 cut(s) 1288, 1716, 2160
BbvCI CCTCAGC 1 cut(s) 126
BceAI ACGGC 1 cut(s) 557
BcgI CGANNNNNNTGC 4 cut(s) 1040, 1074, 2123, 2157
BciT130I CCWGG 7 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373
BcnI CCSGG 1 cut(s) 2485
BcoDI GTCTC 5 cut(s) 113, 918, 1490, 2109, 2349
BfaI CTAG 5 cut(s) 455, 1247, 1479, 1625, 1746
BfmI CTRYAG 3 cut(s) 372, 1143, 1389
BfoI RGCGCY 1 cut(s) 1336
BglI GCCNNNNNGGC 2 cut(s) 248, 2747
BlpI GCTNAGC 2 cut(s) 1365, 2406
Bme1390I CCNGG 8 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373, 2485
Bme18I GGWCC 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 1101
BmgT120I GGNCC 4 cut(s) 412, 712, 2323, 2481
BmiI GGNNCC 5 cut(s) 399, 528, 1074, 1435, 1597
BmrFI CCNGG 8 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373, 2485
BmrI ACTGGG 1 cut(s) 1554
BmsI GCATC 9 cut(s) 16, 433, 496, 625, 1117, 1143, 1777, 1822, 1831
BmuI ACTGGG 1 cut(s) 1554
BpmI CTGGAG 3 cut(s) 1093, 1136, 1452
Bpu10I CCTNAGC 1 cut(s) 126
Bpu1102I GCTNAGC 2 cut(s) 1365, 2406
BpuEI CTTGAG 1 cut(s) 2270
BpuMI CCSGG 1 cut(s) 2485
BsaAI YACGTR 2 cut(s) 1178, 2806
BsaHI GRCGYC 1 cut(s) 1579
BsaI GGTCTC 3 cut(s) 113, 918, 2349
BsaJI CCNNGG 6 cut(s) 408, 586, 587, 1439, 2484, 2814
BsaWI WCCGGW 3 cut(s) 283, 653, 2686
BsaXI ACNNNNNCTCC 2 cut(s) 1515, 1545
Bsc4I CCNNNNNNNGG 2 cut(s) 593, 2463
Bse118I RCCGGY 2 cut(s) 362, 653
Bse1I ACTGG 9 cut(s) 544, 1009, 1119, 1549, 1560, 1579, 1774, 1914, 2152
Bse21I CCTNAGG 1 cut(s) 1167
Bse3DI GCAATG 4 cut(s) 252, 645, 762, 2666
BseBI CCWGG 7 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373
BseDI CCNNGG 6 cut(s) 408, 586, 587, 1439, 2484, 2814
BseGI GGATG 8 cut(s) 31, 598, 780, 988, 1158, 1596, 1645, 1941
BseLI CCNNNNNNNGG 2 cut(s) 593, 2463
BseMI GCAATG 4 cut(s) 252, 645, 762, 2666
BseMII CTCAG 7 cut(s) 80, 117, 124, 1356, 2420, 2549, 2754
BseNI ACTGG 9 cut(s) 544, 1009, 1119, 1549, 1560, 1579, 1774, 1914, 2152
BseRI GAGGAG 1 cut(s) 2097
BseSI GKGCMC 2 cut(s) 1288, 2653
BseYI CCCAGC 1 cut(s) 1697
BsgI GTGCAG 2 cut(s) 534, 1512
BshFI GGCC 7 cut(s) 251, 714, 1884, 2132, 2324, 2483, 2750
BshTI ACCGGT 1 cut(s) 653
BsiHKAI GWGCWC 3 cut(s) 1288, 1716, 2160
BsiHKCI CYCGRG 1 cut(s) 1101
BsiSI CCGG 5 cut(s) 284, 363, 654, 2484, 2687
BslI CCNNNNNNNGG 2 cut(s) 593, 2463
BsmAI GTCTC 5 cut(s) 113, 918, 1490, 2109, 2349
BsmBI CGTCTC 1 cut(s) 2109
BsnI GGCC 7 cut(s) 251, 714, 1884, 2132, 2324, 2483, 2750
Bso31I GGTCTC 3 cut(s) 113, 918, 2349
BsoBI CYCGRG 1 cut(s) 1101
Bsp1286I GDGCHC 6 cut(s) 1288, 1438, 1716, 2160, 2653, 2835
Bsp1720I GCTNAGC 2 cut(s) 1365, 2406
BspACI CCGC 2 cut(s) 2027, 2562
BspANI GGCC 7 cut(s) 251, 714, 1884, 2132, 2324, 2483, 2750
BspCNI CTCAG 7 cut(s) 81, 118, 123, 1357, 2419, 2550, 2755
BspHI TCATGA 2 cut(s) 2073, 2541
BspLI GGNNCC 5 cut(s) 399, 528, 1074, 1435, 1597
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 9 cut(s) 521, 534, 1067, 1080, 1157, 1557, 2047, 2474, 2583
BspQI GCTCTTC 1 cut(s) 2752
BspTNI GGTCTC 3 cut(s) 113, 918, 2349
BsrBI CCGCTC 1 cut(s) 2562
BsrDI GCAATG 4 cut(s) 252, 645, 762, 2666
BsrFI RCCGGY 2 cut(s) 362, 653
BsrI ACTGG 9 cut(s) 544, 1009, 1119, 1549, 1560, 1579, 1774, 1914, 2152
BssAI RCCGGY 2 cut(s) 362, 653
BssECI CCNNGG 6 cut(s) 408, 586, 587, 1439, 2484, 2814
BssNI GRCGYC 1 cut(s) 1579
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 7 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373
Bst4CI ACNGT 9 cut(s) 46, 886, 1033, 1084, 1490, 2103, 2185, 2343, 2866
Bst6I CTCTTC 4 cut(s) 66, 960, 2739, 2752
BstACI GRCGYC 1 cut(s) 1579
BstAPI GCANNNNNTGC 3 cut(s) 1841, 2435, 2677
BstBAI YACGTR 2 cut(s) 1178, 2806
BstC8I GCNNGC 9 cut(s) 976, 1158, 1312, 1739, 1743, 1849, 2001, 2130, 2283
BstDEI CTNAG 9 cut(s) 89, 110, 126, 1167, 1365, 1886, 2406, 2558, 2763
BstDSI CCRYGG 2 cut(s) 1439, 2814
BstF5I GGATG 8 cut(s) 31, 598, 780, 988, 1158, 1596, 1645, 1941
BstH2I RGCGCY 1 cut(s) 1336
BstHHI GCGC 1 cut(s) 1335
BstMAI GTCTC 5 cut(s) 113, 918, 1490, 2109, 2349
BstNI CCWGG 7 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373
BstSCI CCNGG 8 cut(s) 460, 586, 826, 1398, 1879, 2319, 2371, 2483
BstSFI CTRYAG 3 cut(s) 372, 1143, 1389
BstSLI GKGCMC 2 cut(s) 1288, 2653
BstX2I RGATCY 3 cut(s) 526, 1072, 2575
BstXI CCANNNNNNTGG 3 cut(s) 288, 769, 2121
BstYI RGATCY 3 cut(s) 526, 1072, 2575
Bsu36I CCTNAGG 1 cut(s) 1167
BsuRI GGCC 7 cut(s) 251, 714, 1884, 2132, 2324, 2483, 2750
BtgI CCRYGG 2 cut(s) 1439, 2814
BtsCI GGATG 8 cut(s) 31, 598, 780, 988, 1158, 1596, 1645, 1941
BtsI GCAGTG 4 cut(s) 121, 645, 1000, 1858
BtsIMutI CAGTG 6 cut(s) 121, 645, 1000, 1495, 1858, 2792
Cac8I GCNNGC 9 cut(s) 976, 1158, 1312, 1739, 1743, 1849, 2001, 2130, 2283
CaiI CAGNNNCTG 2 cut(s) 1082, 1844
CciI TCATGA 2 cut(s) 2073, 2541
CfoI GCGC 1 cut(s) 1335
Cfr10I RCCGGY 2 cut(s) 362, 653
Cfr13I GGNCC 4 cut(s) 412, 712, 2323, 2481
Csp6I GTAC 3 cut(s) 799, 1606, 2067
CspAI ACCGGT 1 cut(s) 653
CspCI CAANNNNNGTGG 6 cut(s) 690, 725, 1050, 1085, 2407, 2442
CviQI GTAC 3 cut(s) 799, 1606, 2067
DdeI CTNAG 9 cut(s) 89, 110, 126, 1167, 1365, 1886, 2406, 2558, 2763
DrdI GACNNNNNNGTC 1 cut(s) 889
DseDI GACNNNNNNGTC 1 cut(s) 889
EaeI YGGCCR 2 cut(s) 249, 2130
Eam1104I CTCTTC 4 cut(s) 66, 960, 2739, 2752
EarI CTCTTC 4 cut(s) 66, 960, 2739, 2752
EciI GGCGGA 1 cut(s) 2016
Eco130I CCWWGG 1 cut(s) 408
Eco24I GRGCYC 2 cut(s) 1438, 2835
Eco31I GGTCTC 3 cut(s) 113, 918, 2349
Eco32I GATATC 1 cut(s) 2530
Eco47I GGWCC 1 cut(s) 412
Eco57I CTGAAG 2 cut(s) 36, 2816
Eco72I CACGTG 1 cut(s) 1178
Eco81I CCTNAGG 1 cut(s) 1167
Eco88I CYCGRG 1 cut(s) 1101
EcoO109I RGGNCCY 2 cut(s) 412, 2323
EcoRI GAATTC 2 cut(s) 864, 1185
EcoRII CCWGG 7 cut(s) 460, 586, 826, 1398, 1879, 2319, 2371
EcoRV GATATC 1 cut(s) 2530
EcoT14I CCWWGG 1 cut(s) 408
EcoT22I ATGCAT 1 cut(s) 1269
EcoT38I GRGCYC 2 cut(s) 1438, 2835
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 2109
FalI AAGNNNNNCTT 2 cut(s) 2006, 2038
FauI CCCGC 1 cut(s) 2555
FauNDI CATATG 1 cut(s) 755
FblI GTMKAC 1 cut(s) 514
FokI GGATG 8 cut(s) 38, 605, 787, 995, 1165, 1603, 1652, 1948
FriOI GRGCYC 2 cut(s) 1438, 2835
FspBI CTAG 5 cut(s) 455, 1247, 1479, 1625, 1746
GlaI GCGC 1 cut(s) 1334
GsaI CCCAGC 1 cut(s) 1701
GsuI CTGGAG 3 cut(s) 1093, 1136, 1452
HaeII RGCGCY 1 cut(s) 1336
HaeIII GGCC 7 cut(s) 251, 714, 1884, 2132, 2324, 2483, 2750
HapII CCGG 5 cut(s) 284, 363, 654, 2484, 2687
HhaI GCGC 1 cut(s) 1335
Hin1I GRCGYC 1 cut(s) 1579
Hin6I GCGC 1 cut(s) 1333
HinP1I GCGC 1 cut(s) 1333
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HindIII AAGCTT 4 cut(s) 158, 482, 1025, 2279
HpaII CCGG 5 cut(s) 284, 363, 654, 2484, 2687
HphI GGTGA 8 cut(s) 130, 476, 500, 572, 677, 1784, 2500, 2830
Hpy166II GTNNAC 5 cut(s) 515, 1036, 1286, 1376, 2703
Hpy188I TCNGA 8 cut(s) 90, 1014, 1324, 1861, 2162, 2277, 2559, 2736
Hpy8I GTNNAC 5 cut(s) 515, 1036, 1286, 1376, 2703
Hpy99I CGWCG 1 cut(s) 1584
HpyCH4III ACNGT 9 cut(s) 46, 886, 1033, 1084, 1490, 2103, 2185, 2343, 2866
HpyCH4IV ACGT 5 cut(s) 1177, 1579, 2548, 2635, 2805
HpyF3I CTNAG 9 cut(s) 89, 110, 126, 1167, 1365, 1886, 2406, 2558, 2763
HpySE526I ACGT 5 cut(s) 1177, 1579, 2548, 2635, 2805
Hsp92I GRCGYC 1 cut(s) 1579
HspAI GCGC 1 cut(s) 1333
LguI GCTCTTC 1 cut(s) 2752
LmnI GCTCC 4 cut(s) 403, 935, 1433, 1795
LweI GCATC 9 cut(s) 16, 433, 496, 625, 1117, 1143, 1777, 1822, 1831
MaeI CTAG 5 cut(s) 455, 1247, 1479, 1625, 1746
MaeII ACGT 5 cut(s) 1177, 1579, 2548, 2635, 2805
MaeIII GTNAC 6 cut(s) 488, 574, 895, 2335, 2509, 2801
MbiI CCGCTC 1 cut(s) 2562
MboII GAAGA 8 cut(s) 83, 176, 977, 1546, 1918, 2212, 2756, 2769
MfeI CAATTG 3 cut(s) 738, 870, 960
MflI RGATCY 3 cut(s) 526, 1072, 2575
MhlI GDGCHC 6 cut(s) 1288, 1438, 1716, 2160, 2653, 2835
MlsI TGGCCA 1 cut(s) 251
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 4 cut(s) 129, 307, 1652, 1788
Mox20I TGGCCA 1 cut(s) 251
Mph1103I ATGCAT 1 cut(s) 1269
MscI TGGCCA 1 cut(s) 251
MseI TTAA 4 cut(s) 480, 1514, 2097, 2869
MslI CAYNNNNRTG 4 cut(s) 767, 1488, 1758, 2696
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 2 cut(s) 503, 2410
MspI CCGG 5 cut(s) 284, 363, 654, 2484, 2687
MspR9I CCNGG 8 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373, 2485
MunI CAATTG 3 cut(s) 738, 870, 960
MvaI CCWGG 7 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373
NciI CCSGG 1 cut(s) 2485
NdeI CATATG 1 cut(s) 755
NlaIV GGNNCC 5 cut(s) 399, 528, 1074, 1435, 1597
NmeAIII GCCGAG 2 cut(s) 1361, 2158
NmuCI GTSAC 5 cut(s) 488, 574, 895, 2509, 2801
NsiI ATGCAT 1 cut(s) 1269
PaeR7I CTCGAG 1 cut(s) 1101
PagI TCATGA 2 cut(s) 2073, 2541
PasI CCCWGGG 1 cut(s) 587
PciSI GCTCTTC 1 cut(s) 2752
PcsI WCGNNNNNNNCGW 1 cut(s) 2811
PfeI GAWTC 7 cut(s) 85, 211, 563, 805, 1120, 1303, 2810
PfoI TCCNGGA 1 cut(s) 826
PinAI ACCGGT 1 cut(s) 653
PleI GAGTC 4 cut(s) 129, 307, 1651, 1787
PmaCI CACGTG 1 cut(s) 1178
PmlI CACGTG 1 cut(s) 1178
PpsI GAGTC 4 cut(s) 129, 307, 1651, 1787
Ppu21I YACGTR 2 cut(s) 1178, 2806
PpuMI RGGWCCY 1 cut(s) 412
PsiI TTATAA 2 cut(s) 2399, 2502
Psp5II RGGWCCY 1 cut(s) 412
Psp6I CCWGG 7 cut(s) 460, 586, 826, 1398, 1879, 2319, 2371
PspCI CACGTG 1 cut(s) 1178
PspFI CCCAGC 1 cut(s) 1697
PspGI CCWGG 7 cut(s) 460, 586, 826, 1398, 1879, 2319, 2371
PspN4I GGNNCC 5 cut(s) 399, 528, 1074, 1435, 1597
PspPI GGNCC 4 cut(s) 412, 712, 2323, 2481
PspPPI RGGWCCY 1 cut(s) 412
PspXI VCTCGAGB 1 cut(s) 1101
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 2 cut(s) 1082, 1844
PsuI RGATCY 3 cut(s) 526, 1072, 2575
PvuII CAGCTG 2 cut(s) 503, 2410
RsaI GTAC 3 cut(s) 800, 1607, 2068
RsaNI GTAC 3 cut(s) 799, 1606, 2067
RseI CAYNNNNRTG 4 cut(s) 767, 1488, 1758, 2696
SapI GCTCTTC 1 cut(s) 2752
SaqAI TTAA 4 cut(s) 480, 1514, 2097, 2869
Sau96I GGNCC 4 cut(s) 412, 712, 2323, 2481
SchI GAGTC 4 cut(s) 129, 307, 1652, 1788
ScrFI CCNGG 8 cut(s) 462, 588, 828, 1400, 1881, 2321, 2373, 2485
SduI GDGCHC 6 cut(s) 1288, 1438, 1716, 2160, 2653, 2835
SfaNI GCATC 9 cut(s) 16, 433, 496, 625, 1117, 1143, 1777, 1822, 1831
SfcI CTRYAG 3 cut(s) 372, 1143, 1389
Sfr274I CTCGAG 1 cut(s) 1101
SgrAI CRCCGGYG 1 cut(s) 653
SinI GGWCC 1 cut(s) 412
SlaI CTCGAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 4 cut(s) 767, 1488, 1758, 2696
SmlI CTYRAG 2 cut(s) 1101, 2249
SmoI CTYRAG 2 cut(s) 1101, 2249
SsiI CCGC 2 cut(s) 2027, 2562
SspMI CTAG 5 cut(s) 455, 1247, 1479, 1625, 1746
StyD4I CCNGG 8 cut(s) 460, 586, 826, 1398, 1879, 2319, 2371, 2483
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 9 cut(s) 46, 886, 1033, 1084, 1490, 2103, 2185, 2343, 2866
TaiI ACGT 5 cut(s) 1180, 1582, 2551, 2638, 2808
TaqI TCGA 8 cut(s) 418, 1050, 1102, 1329, 1646, 2347, 2835, 2841
TaqII GACCGA 1 cut(s) 828
TfiI GAWTC 7 cut(s) 85, 211, 563, 805, 1120, 1303, 2810
Tru1I TTAA 4 cut(s) 480, 1514, 2097, 2869
Tru9I TTAA 4 cut(s) 480, 1514, 2097, 2869
TscAI CASTG 6 cut(s) 121, 645, 1000, 1495, 1858, 2799
TseFI GTSAC 5 cut(s) 488, 574, 895, 2509, 2801
Tsp45I GTSAC 5 cut(s) 488, 574, 895, 2509, 2801
TspDTI ATGAA 8 cut(s) 334, 450, 1331, 1602, 1952, 1981, 2023, 2530
TspGWI ACGGA 1 cut(s) 2803
TspRI CASTG 6 cut(s) 121, 645, 1000, 1495, 1858, 2799
VneI GTGCAC 1 cut(s) 1284
VpaK11BI GGWCC 1 cut(s) 412
XapI RAATTY 5 cut(s) 182, 745, 864, 1185, 2537
XbaI TCTAGA 1 cut(s) 1246
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XhoI CTCGAG 1 cut(s) 1101
XmiI GTMKAC 1 cut(s) 514
XspI CTAG 5 cut(s) 455, 1247, 1479, 1625, 1746
ZraI GACGTC 1 cut(s) 1580
Zsp2I ATGCAT 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.