FvH4_6g50800

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011712
Physical Location & Seq
Reverse (-)
37762823 .. 37763626
804 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50800.t1

Sequence Viewer

Length: 804 bp
ATGCAAAACCAGGATTATTATTCCGACCAGGGTTTGGTCTGCCCTAGCTTCAACACCTACTCCTCCCCCTCCACCGCCGACACCGCCGCCAAGGTTTGCCGCGAATTCAGAGGCCTCGGCTTAGTCGACGAGGACAACGAGTACGAGTATGATCAAACCGATAAGGAACAAGAAGAGGACGATGACTTCGAGTTCGTTTCGTTTCAGAAATCCGCCGACGAGGTTTTCTTCGGCGACAACACACGGATCGGTCCGGTTTTCCCCGTCTTTAATCGTGATCTTATAAGTCAAAGGGATCTTAACGACGCCGCGTCGTCGTCGGCTTCATCCGACGTGGACGAGCTGGAGGGCGTACCGGCGGGCAGCTACTGCGTTTGGATGCCGAAAACGACGCCGGCATCGTCTCCTTCCCATTGCAAGAAGAGCAAGTCGACCGGAACGTCGTCGTCTCGGAAATGGAGGCTGAGGGACTTGCTGCGGCGGAGCAACAGCGAAGGTGGGAAGGACGCCTTCGTGCTGCTGACGCCTGCACTGACGTCACCACCGAGCTCGAAGAAGCTAACTGGTAACGATCATCATCACCCTAATGAGAAAATGGTAATGACGAAAGGGAAGAAGAGCTTTGATGGGCGGCCGAGATCGAAAGGGCCGAACGCGGTGTCGATGGCGCACGAGGTGTTCTACGCGAAGAGTAAAGAGAAAGAAGGGTACAACAAGAGACAGTCGTTTCTTCCTTACAGGAAAGACCTGGTGGGATTCTTTGCTAATGTGAACGCCATGGGAAGGACCTTTCCTACTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

268

Amino Acids

29.86

Weight (kDa)

6.24

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 72 - 245 1.9e-20 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011712)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27880
fragaria_vesca FvH4_6g50800
malus_domestica MD09G1032200.v1.1
prunus_persica Prupe.3G285800_v2.0.a1
pyrus_communis pycom111g02550 pycom17g03100
rosa_chinensis RchiOBHm_Chr2g0172191
rosa_laevigata RLG00000022091 RLG00000035990
rosa_multiflora Rmu_ssc0000252.1_g000049
rosa_roxburghii Rroxscaffold_2G00121460
rosa_rugosa Rorug02G0556900
rosa_samantha Rh2AG632500 Rh2BG646100 Rh2CG612700 Rh2DG653500 Rh2DG660700 Rh5DG040300
rosa_wichuraiana Rw2G052360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
AasI GACNNNNNNGTC 1 cut(s) 439
AatII GACGTC 1 cut(s) 539
AccB7I CCANNNNNTGG 1 cut(s) 34
AccI GTMKAC 2 cut(s) 126, 431
AccII CGCG 4 cut(s) 102, 311, 656, 686
AclWI GGATC 2 cut(s) 254, 303
AcoI YGGCCR 1 cut(s) 632
AcsI RAATTY 1 cut(s) 104
AcyI GRCGYC 5 cut(s) 306, 392, 507, 524, 536
AdeI CACNNNGTG 1 cut(s) 676
AfaI GTAC 3 cut(s) 143, 354, 710
AfiI CCNNNNNNNGG 2 cut(s) 34, 783
AgsI TTSAA 1 cut(s) 52
AhdI GACNNNNNGTC 1 cut(s) 310
AjiI CACGTC 1 cut(s) 334
AjnI CCWGG 3 cut(s) 9, 27, 747
AluBI AGCT 6 cut(s) 48, 343, 366, 549, 559, 621
AluI AGCT 6 cut(s) 48, 343, 366, 549, 559, 621
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 3 cut(s) 408, 453, 712
AlwI GGATC 2 cut(s) 254, 303
AlwNI CAGNNNCTG 1 cut(s) 369
AoxI GGCC 3 cut(s) 112, 632, 647
ApeKI GCWGC 3 cut(s) 363, 475, 517
ApoI RAATTY 1 cut(s) 104
ArsI GACNNNNNNTTYG 6 cut(s) 170, 176, 202, 208, 644, 676
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 3 cut(s) 251, 647, 786
AsuHPI GGTGA 2 cut(s) 531, 572
AvaII GGWCC 2 cut(s) 251, 786
BanII GRGCYC 1 cut(s) 551
BauI CACGAG 1 cut(s) 671
Bbv12I GWGCWC 1 cut(s) 551
BbvCI CCTCAGC 1 cut(s) 464
BbvI GCAGC 3 cut(s) 375, 462, 504
BccI CCATC 2 cut(s) 620, 658
BciT130I CCWGG 3 cut(s) 11, 29, 749
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 3 cut(s) 408, 453, 712
BfaI CTAG 1 cut(s) 45
BisI GCNGC 8 cut(s) 87, 100, 309, 364, 476, 479, 518, 632
BlsI GCNGC 8 cut(s) 88, 101, 310, 365, 477, 480, 519, 633
Bme1390I CCNGG 3 cut(s) 11, 29, 749
Bme18I GGWCC 2 cut(s) 251, 786
BmeRI GACNNNNNGTC 1 cut(s) 310
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 251, 647, 786
BmrFI CCNGG 3 cut(s) 11, 29, 749
BmsI GCATC 2 cut(s) 369, 407
BpmI CTGGAG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 576
BsaHI GRCGYC 5 cut(s) 306, 392, 507, 524, 536
BsaJI CCNNGG 4 cut(s) 28, 90, 115, 777
BsaWI WCCGGW 2 cut(s) 253, 434
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 783
Bse118I RCCGGY 2 cut(s) 355, 394
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 3 cut(s) 11, 29, 749
BseDI CCNNGG 4 cut(s) 28, 90, 115, 777
BseGI GGATG 2 cut(s) 326, 384
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 34, 783
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 1 cut(s) 52
BseX3I CGGCCG 1 cut(s) 632
BseXI GCAGC 3 cut(s) 375, 462, 504
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 4 cut(s) 102, 311, 656, 686
Bsh1285I CGRYCG 2 cut(s) 435, 635
BshFI GGCC 3 cut(s) 114, 634, 649
BsiEI CGRYCG 2 cut(s) 435, 635
BsiHKAI GWGCWC 1 cut(s) 551
BsiSI CCGG 4 cut(s) 254, 356, 395, 435
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 2 cut(s) 34, 783
BsmAI GTCTC 3 cut(s) 408, 453, 712
BsmBI CGTCTC 2 cut(s) 408, 453
BsmFI GGGAC 1 cut(s) 482
BsnI GGCC 3 cut(s) 114, 634, 649
Bsp1286I GDGCHC 1 cut(s) 551
Bsp143I GATC 6 cut(s) 151, 246, 277, 295, 571, 638
Bsp19I CCATGG 1 cut(s) 777
BspANI GGCC 3 cut(s) 114, 634, 649
BspCNI CTCAG 1 cut(s) 456
BspFNI CGCG 4 cut(s) 102, 311, 656, 686
BspPI GGATC 2 cut(s) 254, 303
BspQI GCTCTTC 2 cut(s) 416, 611
BsrDI GCAATG 1 cut(s) 412
BsrFI RCCGGY 2 cut(s) 355, 394
BsrI ACTGG 1 cut(s) 568
BssAI RCCGGY 2 cut(s) 355, 394
BssECI CCNNGG 4 cut(s) 28, 90, 115, 777
BssMI GATC 6 cut(s) 151, 246, 277, 295, 571, 638
BssNI GRCGYC 5 cut(s) 306, 392, 507, 524, 536
BssSI CACGAG 1 cut(s) 671
BssT1I CCWWGG 2 cut(s) 90, 777
Bst2BI CACGAG 1 cut(s) 671
Bst2UI CCWGG 3 cut(s) 11, 29, 749
Bst4CI ACNGT 1 cut(s) 723
Bst6I CTCTTC 4 cut(s) 168, 416, 611, 683
BstACI GRCGYC 5 cut(s) 306, 392, 507, 524, 536
BstAPI GCANNNNNTGC 1 cut(s) 369
BstC8I GCNNGC 3 cut(s) 361, 396, 528
BstDEI CTNAG 2 cut(s) 121, 464
BstDSI CCRYGG 1 cut(s) 777
BstF5I GGATG 2 cut(s) 326, 384
BstFNI CGCG 4 cut(s) 102, 311, 656, 686
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 6 cut(s) 154, 249, 280, 298, 574, 641
BstMAI GTCTC 3 cut(s) 408, 453, 712
BstMBI GATC 6 cut(s) 151, 246, 277, 295, 571, 638
BstMCI CGRYCG 2 cut(s) 435, 635
BstMWI GCNNNNNNNGC 4 cut(s) 83, 369, 423, 523
BstNI CCWGG 3 cut(s) 11, 29, 749
BstSCI CCNGG 3 cut(s) 9, 27, 747
BstUI CGCG 4 cut(s) 102, 311, 656, 686
BstV1I GCAGC 3 cut(s) 375, 462, 504
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZI CGGCCG 1 cut(s) 632
BsuRI GGCC 3 cut(s) 114, 634, 649
BtgI CCRYGG 1 cut(s) 777
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 2 cut(s) 326, 384
BtsIMutI CAGTG 1 cut(s) 530
Cac8I GCNNGC 3 cut(s) 361, 396, 528
CaiI CAGNNNCTG 1 cut(s) 369
CfoI GCGC 1 cut(s) 670
Cfr10I RCCGGY 2 cut(s) 355, 394
Cfr13I GGNCC 3 cut(s) 251, 647, 786
CpoI CGGWCCG 1 cut(s) 251
CseI GACGC 5 cut(s) 300, 314, 400, 515, 532
CsiI ACCWGGT 1 cut(s) 747
Csp6I GTAC 3 cut(s) 142, 353, 709
CspI CGGWCCG 1 cut(s) 251
CviAII CATG 1 cut(s) 778
CviQI GTAC 3 cut(s) 142, 353, 709
DdeI CTNAG 2 cut(s) 121, 464
DpnI GATC 6 cut(s) 153, 248, 279, 297, 573, 640
DpnII GATC 6 cut(s) 151, 246, 277, 295, 571, 638
DraIII CACNNNGTG 1 cut(s) 676
DrdI GACNNNNNNGTC 1 cut(s) 439
DriI GACNNNNNGTC 1 cut(s) 310
DseDI GACNNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 632
EagI CGGCCG 1 cut(s) 632
Eam1104I CTCTTC 4 cut(s) 168, 416, 611, 683
Eam1105I GACNNNNNGTC 1 cut(s) 310
EarI CTCTTC 4 cut(s) 168, 416, 611, 683
EciI GGCGGA 2 cut(s) 202, 496
Ecl136II GAGCTC 1 cut(s) 549
EclXI CGGCCG 1 cut(s) 632
Eco130I CCWWGG 2 cut(s) 90, 777
Eco147I AGGCCT 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 551
Eco47I GGWCC 2 cut(s) 251, 786
Eco52I CGGCCG 1 cut(s) 632
Eco53kI GAGCTC 1 cut(s) 549
EcoICRI GAGCTC 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 786
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 3 cut(s) 9, 27, 747
EcoT14I CCWWGG 2 cut(s) 90, 777
EcoT38I GRGCYC 1 cut(s) 551
ErhI CCWWGG 2 cut(s) 90, 777
Esp3I CGTCTC 2 cut(s) 408, 453
FaeI CATG 1 cut(s) 781
FaiI YATR 3 cut(s) 150, 284, 779
FalI AAGNNNNNCTT 4 cut(s) 494, 526, 605, 637
FaqI GGGAC 1 cut(s) 482
FatI CATG 1 cut(s) 777
FauI CCCGC 1 cut(s) 352
FbaI TGATCA 1 cut(s) 151
FblI GTMKAC 2 cut(s) 126, 431
Fnu4HI GCNGC 8 cut(s) 87, 100, 309, 364, 476, 479, 518, 632
FokI GGATG 2 cut(s) 313, 391
FriOI GRGCYC 1 cut(s) 551
Fsp4HI GCNGC 8 cut(s) 87, 100, 309, 364, 476, 479, 518, 632
FspBI CTAG 1 cut(s) 45
GlaI GCGC 1 cut(s) 669
GluI GCNGC 8 cut(s) 87, 100, 309, 364, 476, 479, 518, 632
GsuI CTGGAG 1 cut(s) 365
HaeIII GGCC 3 cut(s) 114, 634, 649
HapII CCGG 4 cut(s) 254, 356, 395, 435
HgaI GACGC 5 cut(s) 300, 314, 400, 515, 532
HhaI GCGC 1 cut(s) 670
Hin1I GRCGYC 5 cut(s) 306, 392, 507, 524, 536
Hin1II CATG 1 cut(s) 781
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 2 cut(s) 127, 432
HindII GTYRAC 2 cut(s) 127, 432
HinfI GANTC 1 cut(s) 756
HpaII CCGG 4 cut(s) 254, 356, 395, 435
HphI GGTGA 2 cut(s) 531, 572
Hpy166II GTNNAC 4 cut(s) 127, 337, 432, 772
Hpy188I TCNGA 5 cut(s) 25, 110, 207, 331, 453
Hpy188III TCNNGA 1 cut(s) 275
Hpy8I GTNNAC 4 cut(s) 127, 337, 432, 772
HpyAV CCTTC 6 cut(s) 417, 488, 496, 520, 698, 777
HpyCH4III ACNGT 1 cut(s) 723
HpyCH4IV ACGT 3 cut(s) 333, 440, 536
HpyCH4V TGCA 3 cut(s) 4, 417, 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 369, 423, 523
HpyF3I CTNAG 2 cut(s) 121, 464
HpySE526I ACGT 3 cut(s) 333, 440, 536
Hsp92I GRCGYC 5 cut(s) 306, 392, 507, 524, 536
Hsp92II CATG 1 cut(s) 781
HspAI GCGC 1 cut(s) 668
KroI GCCGGC 1 cut(s) 394
KroNI GCCGGC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 6 cut(s) 151, 246, 277, 295, 571, 638
LguI GCTCTTC 2 cut(s) 416, 611
LmnI GCTCC 1 cut(s) 483
Lsp1109I GCAGC 3 cut(s) 375, 462, 504
LweI GCATC 2 cut(s) 369, 407
MabI ACCWGGT 1 cut(s) 747
MaeI CTAG 1 cut(s) 45
MaeII ACGT 3 cut(s) 333, 440, 536
MaeIII GTNAC 2 cut(s) 537, 566
MalI GATC 6 cut(s) 153, 248, 279, 297, 573, 640
MboI GATC 6 cut(s) 151, 246, 277, 295, 571, 638
MboII GAAGA 8 cut(s) 185, 220, 433, 565, 625, 628, 700, 722
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 551
MluCI AATT 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 48, 354
MroNI GCCGGC 1 cut(s) 394
MseI TTAA 2 cut(s) 270, 300
MslI CAYNNNNRTG 1 cut(s) 585
MspI CCGG 4 cut(s) 254, 356, 395, 435
MspR9I CCNGG 3 cut(s) 11, 29, 749
MvaI CCWGG 3 cut(s) 11, 29, 749
MvnI CGCG 4 cut(s) 102, 311, 656, 686
MwoI GCNNNNNNNGC 4 cut(s) 83, 369, 423, 523
NaeI GCCGGC 1 cut(s) 396
NcoI CCATGG 1 cut(s) 777
NdeII GATC 6 cut(s) 151, 246, 277, 295, 571, 638
NgoMIV GCCGGC 1 cut(s) 394
NlaIII CATG 1 cut(s) 781
NmeAIII GCCGAG 2 cut(s) 96, 660
NmuCI GTSAC 1 cut(s) 537
PceI AGGCCT 1 cut(s) 114
PciSI GCTCTTC 2 cut(s) 416, 611
PcsI WCGNNNNNNNCGW 7 cut(s) 123, 135, 186, 398, 437, 542, 647
PdiI GCCGGC 1 cut(s) 396
PfeI GAWTC 1 cut(s) 756
PflMI CCANNNNNTGG 1 cut(s) 34
PkrI GCNGC 8 cut(s) 88, 101, 310, 365, 477, 480, 519, 633
PpuMI RGGWCCY 1 cut(s) 786
PsiI TTATAA 1 cut(s) 284
Psp124BI GAGCTC 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 786
Psp6I CCWGG 3 cut(s) 9, 27, 747
PspGI CCWGG 3 cut(s) 9, 27, 747
PspPI GGNCC 3 cut(s) 251, 647, 786
PspPPI RGGWCCY 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 369
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 3 cut(s) 143, 354, 710
RsaNI GTAC 3 cut(s) 142, 353, 709
RseI CAYNNNNRTG 1 cut(s) 585
Rsr2I CGGWCCG 1 cut(s) 251
RsrII CGGWCCG 1 cut(s) 251
SacI GAGCTC 1 cut(s) 551
SalI GTCGAC 2 cut(s) 125, 430
SapI GCTCTTC 2 cut(s) 416, 611
SaqAI TTAA 2 cut(s) 270, 300
SatI GCNGC 8 cut(s) 87, 100, 309, 364, 476, 479, 518, 632
Sau3AI GATC 6 cut(s) 151, 246, 277, 295, 571, 638
Sau96I GGNCC 3 cut(s) 251, 647, 786
ScrFI CCNGG 3 cut(s) 11, 29, 749
SduI GDGCHC 1 cut(s) 551
SexAI ACCWGGT 1 cut(s) 747
SfaNI GCATC 2 cut(s) 369, 407
SinI GGWCC 2 cut(s) 251, 786
SmiMI CAYNNNNRTG 1 cut(s) 585
Sse9I AATT 1 cut(s) 104
SseBI AGGCCT 1 cut(s) 114
SspMI CTAG 1 cut(s) 45
SstI GAGCTC 1 cut(s) 551
StuI AGGCCT 1 cut(s) 114
StyD4I CCNGG 3 cut(s) 9, 27, 747
StyI CCWWGG 2 cut(s) 90, 777
TaaI ACNGT 1 cut(s) 723
TaiI ACGT 3 cut(s) 336, 443, 539
TaqI TCGA 6 cut(s) 126, 189, 431, 551, 641, 662
TaqII GACCGA 1 cut(s) 239
TasI AATT 1 cut(s) 104
TauI GCSGC 5 cut(s) 89, 102, 311, 481, 634
TfiI GAWTC 1 cut(s) 756
Tru1I TTAA 2 cut(s) 270, 300
Tru9I TTAA 2 cut(s) 270, 300
TscAI CASTG 1 cut(s) 537
TseFI GTSAC 1 cut(s) 537
TseI GCWGC 3 cut(s) 363, 475, 517
Tsp45I GTSAC 1 cut(s) 537
TspDTI ATGAA 1 cut(s) 315
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 1 cut(s) 537
Van91I CCANNNNNTGG 1 cut(s) 34
VpaK11BI GGWCC 2 cut(s) 251, 786
XapI RAATTY 1 cut(s) 104
XmiI GTMKAC 2 cut(s) 126, 431
XspI CTAG 1 cut(s) 45
ZraI GACGTC 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.