RLG00000022091

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011712
Physical Location & Seq
Reverse (-)
80838948 .. 80839766
819 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022091

Sequence Viewer

Length: 819 bp
ATGCAAAACGAAGATTACTCCGACCAGGGTTTGGTCTGTCCCAGCTTCAACAGCTACGCCTCGGCCACCACAGCCGACACCGCCGCCAAGGTTTGCCGGGAATTCAGCAGCCTCGGATTAGTGGATGAGAACAAAGAGTTCGAGTATGAAGGAGGAGAGAAACAAGACGATGACTTCGAGTTCGTGTCGTTTCAGAAATCTGCCGACGAGATTTTCTTCGGCGACAACACTCGGATCGGTCCGGTTTTCCCCGTCTTCAACCGCGACCTTATAAGTCAACGGGATCTAAAGGACACCGATGACGTCGACGTCGACGCCGTGCCGTCGTCGTCGTCCGCGTCATCAGACGTGGACGAGCTGGAGAACGTTCCGGCGGGTAGCTACTGCGTTTGGATGCCGAGAACGGCGCCGGCCTCGTCTCCTTCCCATTGCAAGAAGAGCAAGTCGACCGGAACGTCGTCGTCTCGGCGGTGGAGGCTGAGGGACTTGCTGCGGCGGAGCAACAGCGAAGGCGGGAAGGACGCCTTCGTGCTGCTGACGCCTTCCCTGACGTCATCGTCGAGCTCCAAGAAGCAGGTGGCTGCCAACGATCATCATCATCCTAATGAGAAAATGGTGGTGACGAAGGGGAAGAAGAGCTTTAATGGGTCGAGGCCGAAAGGCCCGAACGCGGTGTCGATGGCGCACGAAGCGTTCTACGCGAAGAGTAAAGAGAAAGATGGGTATAATAAGAGACAGTCGTTTCTTCCTTACAGGAAAGACCTGGTGGGGTTCTTTGCTAGTGTGAACGCCATGGGAAGGACTTTCCCTACTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

273

Amino Acids

30.06

Weight (kDa)

6.24

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 68 - 250 6.5e-24 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011712)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27880
fragaria_vesca FvH4_6g50800
malus_domestica MD09G1032200.v1.1
prunus_persica Prupe.3G285800_v2.0.a1
pyrus_communis pycom111g02550 pycom17g03100
rosa_chinensis RchiOBHm_Chr2g0172191
rosa_laevigata RLG00000022091 RLG00000035990
rosa_multiflora Rmu_ssc0000252.1_g000049
rosa_roxburghii Rroxscaffold_2G00121460
rosa_rugosa Rorug02G0556900
rosa_samantha Rh2AG632500 Rh2BG646100 Rh2CG612700 Rh2DG653500 Rh2DG660700 Rh5DG040300
rosa_wichuraiana Rw2G052360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 272
AarI CACCTGC 1 cut(s) 565
AasI GACNNNNNNGTC 3 cut(s) 308, 454, 556
AatII GACGTC 3 cut(s) 306, 312, 554
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 406
AccB7I CCANNNNNTGG 1 cut(s) 31
AccI GTMKAC 3 cut(s) 306, 312, 446
AccII CGCG 4 cut(s) 264, 338, 671, 701
AclI AACGTT 1 cut(s) 366
AclWI GGATC 2 cut(s) 242, 291
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 101
AcyI GRCGYC 7 cut(s) 303, 309, 315, 407, 522, 539, 551
AfiI CCNNNNNNNGG 3 cut(s) 31, 670, 798
AgsI TTSAA 2 cut(s) 49, 259
AjiI CACGTC 1 cut(s) 349
AjnI CCWGG 2 cut(s) 24, 762
AluBI AGCT 6 cut(s) 45, 54, 358, 381, 564, 639
AluI AGCT 6 cut(s) 45, 54, 358, 381, 564, 639
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 3 cut(s) 423, 468, 727
AlwI GGATC 2 cut(s) 242, 291
AoxI GGCC 4 cut(s) 63, 411, 653, 661
ApeKI GCWGC 4 cut(s) 108, 490, 532, 581
ApoI RAATTY 1 cut(s) 101
ArsI GACNNNNNNTTYG 6 cut(s) 158, 164, 190, 196, 659, 691
AspLEI GCGC 2 cut(s) 409, 685
AspS9I GGNCC 2 cut(s) 239, 662
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 631
AvaII GGWCC 1 cut(s) 239
BanI GGYRCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 566
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 566
BbvCI CCTCAGC 1 cut(s) 479
BbvI GCAGC 4 cut(s) 120, 477, 519, 568
BccI CCATC 2 cut(s) 673, 713
BceAI ACGGC 3 cut(s) 302, 307, 420
BciT130I CCWGG 2 cut(s) 26, 764
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 3 cut(s) 423, 468, 727
BfaI CTAG 1 cut(s) 780
BfoI RGCGCY 1 cut(s) 410
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 6 cut(s) 84, 109, 491, 494, 533, 582
BlsI GCNGC 6 cut(s) 85, 110, 492, 495, 534, 583
Bme1390I CCNGG 3 cut(s) 26, 98, 764
Bme18I GGWCC 1 cut(s) 239
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 2 cut(s) 239, 662
BmiI GGNNCC 1 cut(s) 408
BmrFI CCNGG 3 cut(s) 26, 98, 764
BmsI GCATC 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 380
Bpu10I CCTNAGC 1 cut(s) 479
BpuMI CCSGG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 594
BsaHI GRCGYC 7 cut(s) 303, 309, 315, 407, 522, 539, 551
BsaJI CCNNGG 5 cut(s) 25, 60, 87, 112, 792
BsaWI WCCGGW 2 cut(s) 241, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 670, 798
Bse118I RCCGGY 1 cut(s) 409
Bse3DI GCAATG 1 cut(s) 427
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 2 cut(s) 26, 764
BseDI CCNNGG 5 cut(s) 25, 60, 87, 112, 792
BseGI GGATG 3 cut(s) 130, 399, 598
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 3 cut(s) 31, 670, 798
BseMI GCAATG 1 cut(s) 427
BseMII CTCAG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 4 cut(s) 120, 477, 519, 568
BseYI CCCAGC 1 cut(s) 41
Bsh1236I CGCG 4 cut(s) 264, 338, 671, 701
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 4 cut(s) 65, 413, 655, 663
BshNI GGYRCC 1 cut(s) 406
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 1 cut(s) 566
BsiSI CCGG 5 cut(s) 97, 242, 371, 410, 450
BslFI GGGAC 2 cut(s) 24, 497
BslI CCNNNNNNNGG 3 cut(s) 31, 670, 798
BsmAI GTCTC 3 cut(s) 423, 468, 727
BsmBI CGTCTC 2 cut(s) 423, 468
BsmFI GGGAC 2 cut(s) 24, 497
BsnI GGCC 4 cut(s) 65, 413, 655, 663
Bsp1286I GDGCHC 1 cut(s) 566
Bsp143I GATC 3 cut(s) 234, 283, 589
Bsp19I CCATGG 1 cut(s) 792
BspANI GGCC 4 cut(s) 65, 413, 655, 663
BspCNI CTCAG 1 cut(s) 471
BspFNI CGCG 4 cut(s) 264, 338, 671, 701
BspLI GGNNCC 1 cut(s) 408
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 2 cut(s) 242, 291
BspQI GCTCTTC 2 cut(s) 431, 629
BspT107I GGYRCC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 427
BsrFI RCCGGY 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 409
BssECI CCNNGG 5 cut(s) 25, 60, 87, 112, 792
BssMI GATC 3 cut(s) 234, 283, 589
BssNI GRCGYC 7 cut(s) 303, 309, 315, 407, 522, 539, 551
BssT1I CCWWGG 2 cut(s) 87, 792
Bst2UI CCWGG 2 cut(s) 26, 764
Bst4CI ACNGT 1 cut(s) 738
Bst6I CTCTTC 3 cut(s) 431, 629, 698
BstACI GRCGYC 7 cut(s) 303, 309, 315, 407, 522, 539, 551
BstC8I GCNNGC 1 cut(s) 411
BstDEI CTNAG 1 cut(s) 479
BstDSI CCRYGG 1 cut(s) 792
BstF5I GGATG 3 cut(s) 130, 399, 598
BstFNI CGCG 4 cut(s) 264, 338, 671, 701
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 2 cut(s) 409, 685
BstKTI GATC 3 cut(s) 237, 286, 592
BstMAI GTCTC 3 cut(s) 423, 468, 727
BstMBI GATC 3 cut(s) 234, 283, 589
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 8 cut(s) 51, 71, 80, 438, 475, 538, 689, 698
BstNI CCWGG 2 cut(s) 26, 764
BstSCI CCNGG 3 cut(s) 24, 96, 762
BstUI CGCG 4 cut(s) 264, 338, 671, 701
BstV1I GCAGC 4 cut(s) 120, 477, 519, 568
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 4 cut(s) 65, 413, 655, 663
BtgI CCRYGG 1 cut(s) 792
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 3 cut(s) 130, 399, 598
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 1 cut(s) 411
CfoI GCGC 2 cut(s) 409, 685
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 239, 662
CpoI CGGWCCG 1 cut(s) 239
CseI GACGC 4 cut(s) 323, 327, 530, 547
CsiI ACCWGGT 1 cut(s) 762
CspI CGGWCCG 1 cut(s) 239
CviAII CATG 1 cut(s) 793
DdeI CTNAG 1 cut(s) 479
DinI GGCGCC 1 cut(s) 408
DpnI GATC 3 cut(s) 236, 285, 591
DpnII GATC 3 cut(s) 234, 283, 589
DrdI GACNNNNNNGTC 3 cut(s) 308, 454, 556
DseDI GACNNNNNNGTC 3 cut(s) 308, 454, 556
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 3 cut(s) 431, 629, 698
EarI CTCTTC 3 cut(s) 431, 629, 698
EciI GGCGGA 1 cut(s) 511
Ecl136II GAGCTC 1 cut(s) 564
Eco130I CCWWGG 2 cut(s) 87, 792
Eco24I GRGCYC 1 cut(s) 566
Eco47I GGWCC 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 564
EcoICRI GAGCTC 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 2 cut(s) 24, 762
EcoT14I CCWWGG 2 cut(s) 87, 792
EcoT38I GRGCYC 1 cut(s) 566
EgeI GGCGCC 1 cut(s) 408
EheI GGCGCC 1 cut(s) 408
ErhI CCWWGG 2 cut(s) 87, 792
Esp3I CGTCTC 2 cut(s) 423, 468
FaeI CATG 1 cut(s) 796
FaiI YATR 4 cut(s) 147, 272, 726, 794
FalI AAGNNNNNCTT 4 cut(s) 509, 541, 623, 655
FaqI GGGAC 2 cut(s) 24, 497
FatI CATG 1 cut(s) 792
FauI CCCGC 2 cut(s) 367, 506
FblI GTMKAC 3 cut(s) 306, 312, 446
Fnu4HI GCNGC 6 cut(s) 84, 109, 491, 494, 533, 582
FokI GGATG 3 cut(s) 137, 406, 585
FriOI GRGCYC 1 cut(s) 566
Fsp4HI GCNGC 6 cut(s) 84, 109, 491, 494, 533, 582
FspBI CTAG 1 cut(s) 780
GlaI GCGC 2 cut(s) 408, 684
GluI GCNGC 6 cut(s) 84, 109, 491, 494, 533, 582
GsaI CCCAGC 1 cut(s) 45
GsuI CTGGAG 1 cut(s) 380
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 4 cut(s) 65, 413, 655, 663
HapII CCGG 5 cut(s) 97, 242, 371, 410, 450
HgaI GACGC 4 cut(s) 323, 327, 530, 547
HhaI GCGC 2 cut(s) 409, 685
Hin1I GRCGYC 7 cut(s) 303, 309, 315, 407, 522, 539, 551
Hin1II CATG 1 cut(s) 796
Hin6I GCGC 2 cut(s) 407, 683
HinP1I GCGC 2 cut(s) 407, 683
HincII GTYRAC 4 cut(s) 278, 307, 313, 447
HindII GTYRAC 4 cut(s) 278, 307, 313, 447
HpaII CCGG 5 cut(s) 97, 242, 371, 410, 450
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 6 cut(s) 278, 307, 313, 352, 447, 787
Hpy188I TCNGA 5 cut(s) 22, 116, 195, 234, 346
Hpy8I GTNNAC 6 cut(s) 278, 307, 313, 352, 447, 787
HpyAV CCTTC 8 cut(s) 143, 432, 503, 511, 535, 552, 619, 792
HpyCH4III ACNGT 1 cut(s) 738
HpyCH4IV ACGT 6 cut(s) 303, 309, 348, 366, 455, 551
HpyCH4V TGCA 2 cut(s) 4, 432
HpyF10VI GCNNNNNNNGC 8 cut(s) 51, 71, 80, 438, 475, 538, 689, 698
HpyF3I CTNAG 1 cut(s) 479
HpySE526I ACGT 6 cut(s) 303, 309, 348, 366, 455, 551
Hsp92I GRCGYC 7 cut(s) 303, 309, 315, 407, 522, 539, 551
Hsp92II CATG 1 cut(s) 796
HspAI GCGC 2 cut(s) 407, 683
KasI GGCGCC 1 cut(s) 406
KroI GCCGGC 1 cut(s) 409
KroNI GCCGGC 1 cut(s) 411
Kzo9I GATC 3 cut(s) 234, 283, 589
LguI GCTCTTC 2 cut(s) 431, 629
LmnI GCTCC 2 cut(s) 498, 569
Lsp1109I GCAGC 4 cut(s) 120, 477, 519, 568
LweI GCATC 1 cut(s) 384
MabI ACCWGGT 1 cut(s) 762
MaeI CTAG 1 cut(s) 780
MaeII ACGT 6 cut(s) 303, 309, 348, 366, 455, 551
MaeIII GTNAC 1 cut(s) 619
MalI GATC 3 cut(s) 236, 285, 591
MboI GATC 3 cut(s) 234, 283, 589
MboII GAAGA 8 cut(s) 23, 208, 247, 448, 643, 646, 715, 737
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 566
MluCI AATT 1 cut(s) 101
Mly113I GGCGCC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 7 cut(s) 70, 122, 146, 424, 468, 474, 645
MroNI GCCGGC 1 cut(s) 409
MseI TTAA 2 cut(s) 642, 817
MslI CAYNNNNRTG 1 cut(s) 603
MspI CCGG 5 cut(s) 97, 242, 371, 410, 450
MspR9I CCNGG 3 cut(s) 26, 98, 764
MvaI CCWGG 2 cut(s) 26, 764
MvnI CGCG 4 cut(s) 264, 338, 671, 701
MwoI GCNNNNNNNGC 8 cut(s) 51, 71, 80, 438, 475, 538, 689, 698
NaeI GCCGGC 1 cut(s) 411
NarI GGCGCC 1 cut(s) 407
NciI CCSGG 1 cut(s) 98
NcoI CCATGG 1 cut(s) 792
NdeII GATC 3 cut(s) 234, 283, 589
NgoMIV GCCGGC 1 cut(s) 409
NlaIII CATG 1 cut(s) 796
NlaIV GGNNCC 1 cut(s) 408
NmeAIII GCCGAG 3 cut(s) 41, 423, 445
NmuCI GTSAC 1 cut(s) 619
PaqCI CACCTGC 1 cut(s) 565
PciSI GCTCTTC 2 cut(s) 431, 629
PcsI WCGNNNNNNNCGW 6 cut(s) 174, 309, 315, 335, 452, 557
PdiI GCCGGC 1 cut(s) 411
PflMI CCANNNNNTGG 1 cut(s) 31
PkrI GCNGC 6 cut(s) 85, 110, 492, 495, 534, 583
PluTI GGCGCC 1 cut(s) 410
PsiI TTATAA 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 566
Psp1406I AACGTT 1 cut(s) 366
Psp6I CCWGG 2 cut(s) 24, 762
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 2 cut(s) 24, 762
PspN4I GGNNCC 1 cut(s) 408
PspPI GGNCC 2 cut(s) 239, 662
PsuI RGATCY 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 603
Rsr2I CGGWCCG 1 cut(s) 239
RsrII CGGWCCG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 566
SalI GTCGAC 3 cut(s) 305, 311, 445
SapI GCTCTTC 2 cut(s) 431, 629
SaqAI TTAA 2 cut(s) 642, 817
SatI GCNGC 6 cut(s) 84, 109, 491, 494, 533, 582
Sau3AI GATC 3 cut(s) 234, 283, 589
Sau96I GGNCC 2 cut(s) 239, 662
ScrFI CCNGG 3 cut(s) 26, 98, 764
SduI GDGCHC 1 cut(s) 566
SexAI ACCWGGT 1 cut(s) 762
SfaNI GCATC 1 cut(s) 384
SfoI GGCGCC 1 cut(s) 408
SgrDI CGTCGACG 2 cut(s) 305, 311
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 603
Sse9I AATT 1 cut(s) 101
SspDI GGCGCC 1 cut(s) 406
SspMI CTAG 1 cut(s) 780
SstI GAGCTC 1 cut(s) 566
StyD4I CCNGG 3 cut(s) 24, 96, 762
StyI CCWWGG 2 cut(s) 87, 792
TaaI ACNGT 1 cut(s) 738
TaiI ACGT 6 cut(s) 306, 312, 351, 369, 458, 554
TaqI TCGA 8 cut(s) 141, 177, 306, 312, 446, 560, 650, 677
TaqII GACCGA 1 cut(s) 227
TasI AATT 1 cut(s) 101
TauI GCSGC 2 cut(s) 86, 496
Tru1I TTAA 2 cut(s) 642, 817
Tru9I TTAA 2 cut(s) 642, 817
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 4 cut(s) 108, 490, 532, 581
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 1 cut(s) 162
Van91I CCANNNNNTGG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 1 cut(s) 101
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmiI GTMKAC 3 cut(s) 306, 312, 446
XspI CTAG 1 cut(s) 780
ZraI GACGTC 3 cut(s) 304, 310, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.