Rh2DG653500

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011712
Physical Location & Seq
Reverse (-)
87857149 .. 87857967
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG653500.1

Sequence Viewer

Length: 819 bp
ATGCAAAACGAAGATTACTTCGACCAGGGTTTGGTCTGTCCCAGCTTCAACAGCTACGCCTCGGCCACCACAGCCGACACCGCCGCCAAGGTTTGCCGGGAATTCAGCAGCCTCGGATTAGTGGATGAGAACAAAGAGTTCGAGTATGAAGGAGGAGAGAAACAAGACGATGACTTCGAGTTCGTGTCGTTTCAGAAATCTGCCGACGAGGTTTTCTTCGGCGACAACACTCGGATCGGTCCGGTTTTCCCCGTCTTCAACCGCGACCTTATAAGTCAACGGGATCTAAACGACACCGACGACGTCGACGCCGTGCCGTCGTCGTCCTCAGCGTCATCAGACGTGGACGAGCTGGAGAACGTTCCGGCGGGTAGCTACTGCGTTTGGATGCCGAAAACGACGCCGGCCTCGTCTCCTTCCCATTGCAAGAAGAGCAAGTCGACCGGAACGTCGTCGTCTCGGCGGTGGAGGCTGAGGGACTTGCTGCGGCGGAGCAACAGCGAAGGCGGGAAGGACGCCTTCGTGCTGCTGACGCCTTCCCTGACGTCATCGTCGAGCTCCAAGAAGCTGGTGGCTGCTAACGATCATCATCATCATCATCCTAATGAGAAAATGGTGGTGACGAAGGGGAAGAAGAGCTTTAATGGGTCGAGGACGAAAGGCCCGAACGCGGTGTCGATGGCGCACGAGCTGTTCTACGCGAAGAGTAAAGAGAAAGATGGGTATAATAAGAGACAGTCGTATCTTCCTTACAGGAAAGACCTGGTGGGGTTCTTTGCTAGTGTGAACGCCATGGGAAGGACTTTCCCTACTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

272

Amino Acids

30.2

Weight (kDa)

6.39

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 68 - 250 6.3e-24 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011712)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27880
fragaria_vesca FvH4_6g50800
malus_domestica MD09G1032200.v1.1
prunus_persica Prupe.3G285800_v2.0.a1
pyrus_communis pycom111g02550 pycom17g03100
rosa_chinensis RchiOBHm_Chr2g0172191
rosa_laevigata RLG00000022091 RLG00000035990
rosa_multiflora Rmu_ssc0000252.1_g000049
rosa_roxburghii Rroxscaffold_2G00121460
rosa_rugosa Rorug02G0556900
rosa_samantha Rh2AG632500 Rh2BG646100 Rh2CG612700 Rh2DG653500 Rh2DG660700 Rh5DG040300
rosa_wichuraiana Rw2G052360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 272
AasI GACNNNNNNGTC 2 cut(s) 448, 550
AatII GACGTC 2 cut(s) 306, 548
AccB7I CCANNNNNTGG 1 cut(s) 31
AccI GTMKAC 2 cut(s) 306, 440
AccII CGCG 3 cut(s) 264, 671, 701
AciI CCGC 9 cut(s) 81, 84, 262, 368, 463, 487, 490, 507, 671
AclI AACGTT 1 cut(s) 360
AclWI GGATC 2 cut(s) 242, 291
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 101
AcyI GRCGYC 6 cut(s) 303, 309, 401, 516, 533, 545
AfiI CCNNNNNNNGG 3 cut(s) 31, 670, 798
AgsI TTSAA 2 cut(s) 49, 259
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 2 cut(s) 24, 762
AluBI AGCT 8 cut(s) 45, 54, 352, 375, 558, 568, 639, 691
AluI AGCT 8 cut(s) 45, 54, 352, 375, 558, 568, 639, 691
Alw21I GWGCWC 1 cut(s) 560
Alw26I GTCTC 3 cut(s) 417, 462, 727
AlwI GGATC 2 cut(s) 242, 291
AoxI GGCC 3 cut(s) 63, 405, 661
ApeKI GCWGC 4 cut(s) 108, 484, 526, 575
ApoI RAATTY 1 cut(s) 101
ArsI GACNNNNNNTTYG 6 cut(s) 158, 164, 190, 196, 659, 691
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 2 cut(s) 239, 662
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 631
AvaII GGWCC 1 cut(s) 239
BaeI ACNNNNGTAYC 2 cut(s) 725, 758
BanII GRGCYC 1 cut(s) 560
BauI CACGAG 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 560
BbvCI CCTCAGC 2 cut(s) 328, 473
BbvI GCAGC 4 cut(s) 120, 471, 513, 562
BccI CCATC 2 cut(s) 673, 713
BceAI ACGGC 2 cut(s) 296, 301
BciT130I CCWGG 2 cut(s) 26, 764
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 3 cut(s) 417, 462, 727
BfaI CTAG 1 cut(s) 780
BisI GCNGC 6 cut(s) 84, 109, 485, 488, 527, 576
BlsI GCNGC 6 cut(s) 85, 110, 486, 489, 528, 577
Bme1390I CCNGG 3 cut(s) 26, 98, 764
Bme18I GGWCC 1 cut(s) 239
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 2 cut(s) 239, 662
BmrFI CCNGG 3 cut(s) 26, 98, 764
BmsI GCATC 1 cut(s) 378
BpiI GAAGAC 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 374
Bpu10I CCTNAGC 2 cut(s) 328, 473
BpuMI CCSGG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 588
BsaHI GRCGYC 6 cut(s) 303, 309, 401, 516, 533, 545
BsaJI CCNNGG 5 cut(s) 25, 60, 87, 112, 792
BsaWI WCCGGW 2 cut(s) 241, 443
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 670, 798
Bse118I RCCGGY 1 cut(s) 403
Bse3DI GCAATG 1 cut(s) 421
Bse8I GATNNNNATC 1 cut(s) 588
BseBI CCWGG 2 cut(s) 26, 764
BseDI CCNNGG 5 cut(s) 25, 60, 87, 112, 792
BseGI GGATG 3 cut(s) 130, 393, 598
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 3 cut(s) 31, 670, 798
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 2 cut(s) 342, 464
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 4 cut(s) 120, 471, 513, 562
BseYI CCCAGC 1 cut(s) 41
Bsh1236I CGCG 3 cut(s) 264, 671, 701
Bsh1285I CGRYCG 1 cut(s) 444
BshFI GGCC 3 cut(s) 65, 407, 663
BsiEI CGRYCG 1 cut(s) 444
BsiHKAI GWGCWC 1 cut(s) 560
BsiSI CCGG 5 cut(s) 97, 242, 365, 404, 444
BslFI GGGAC 2 cut(s) 24, 491
BslI CCNNNNNNNGG 3 cut(s) 31, 670, 798
BsmAI GTCTC 3 cut(s) 417, 462, 727
BsmBI CGTCTC 2 cut(s) 417, 462
BsmFI GGGAC 2 cut(s) 24, 491
BsnI GGCC 3 cut(s) 65, 407, 663
Bsp1286I GDGCHC 1 cut(s) 560
Bsp143I GATC 3 cut(s) 234, 283, 583
Bsp19I CCATGG 1 cut(s) 792
BspACI CCGC 9 cut(s) 81, 84, 262, 368, 463, 487, 490, 507, 671
BspANI GGCC 3 cut(s) 65, 407, 663
BspCNI CTCAG 2 cut(s) 341, 465
BspFNI CGCG 3 cut(s) 264, 671, 701
BspPI GGATC 2 cut(s) 242, 291
BspQI GCTCTTC 2 cut(s) 425, 629
BsrDI GCAATG 1 cut(s) 421
BsrFI RCCGGY 1 cut(s) 403
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 5 cut(s) 25, 60, 87, 112, 792
BssMI GATC 3 cut(s) 234, 283, 583
BssNI GRCGYC 6 cut(s) 303, 309, 401, 516, 533, 545
BssSI CACGAG 1 cut(s) 686
BssT1I CCWWGG 2 cut(s) 87, 792
Bst2BI CACGAG 1 cut(s) 686
Bst2UI CCWGG 2 cut(s) 26, 764
Bst4CI ACNGT 1 cut(s) 738
Bst6I CTCTTC 3 cut(s) 425, 629, 698
BstACI GRCGYC 6 cut(s) 303, 309, 401, 516, 533, 545
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 2 cut(s) 328, 473
BstDSI CCRYGG 1 cut(s) 792
BstF5I GGATG 3 cut(s) 130, 393, 598
BstFNI CGCG 3 cut(s) 264, 671, 701
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 3 cut(s) 237, 286, 586
BstMAI GTCTC 3 cut(s) 417, 462, 727
BstMBI GATC 3 cut(s) 234, 283, 583
BstMCI CGRYCG 1 cut(s) 444
BstMWI GCNNNNNNNGC 6 cut(s) 51, 71, 80, 432, 469, 532
BstNI CCWGG 2 cut(s) 26, 764
BstSCI CCNGG 3 cut(s) 24, 96, 762
BstUI CGCG 3 cut(s) 264, 671, 701
BstV1I GCAGC 4 cut(s) 120, 471, 513, 562
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 1 cut(s) 283
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 3 cut(s) 65, 407, 663
BtgI CCRYGG 1 cut(s) 792
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 3 cut(s) 130, 393, 598
Cac8I GCNNGC 1 cut(s) 405
CfoI GCGC 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 239, 662
CpoI CGGWCCG 1 cut(s) 239
CseI GACGC 5 cut(s) 317, 321, 409, 524, 541
CsiI ACCWGGT 1 cut(s) 762
CspI CGGWCCG 1 cut(s) 239
CviAII CATG 1 cut(s) 793
DdeI CTNAG 2 cut(s) 328, 473
DpnI GATC 3 cut(s) 236, 285, 585
DpnII GATC 3 cut(s) 234, 283, 583
DrdI GACNNNNNNGTC 2 cut(s) 448, 550
DseDI GACNNNNNNGTC 2 cut(s) 448, 550
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 3 cut(s) 425, 629, 698
EarI CTCTTC 3 cut(s) 425, 629, 698
EciI GGCGGA 1 cut(s) 505
Ecl136II GAGCTC 1 cut(s) 558
Eco130I CCWWGG 2 cut(s) 87, 792
Eco24I GRGCYC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 558
EcoICRI GAGCTC 1 cut(s) 558
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 2 cut(s) 24, 762
EcoT14I CCWWGG 2 cut(s) 87, 792
EcoT38I GRGCYC 1 cut(s) 560
ErhI CCWWGG 2 cut(s) 87, 792
Esp3I CGTCTC 2 cut(s) 417, 462
FaeI CATG 1 cut(s) 796
FaiI YATR 4 cut(s) 147, 272, 726, 794
FalI AAGNNNNNCTT 4 cut(s) 503, 535, 623, 655
FaqI GGGAC 2 cut(s) 24, 491
FatI CATG 1 cut(s) 792
FauI CCCGC 2 cut(s) 361, 500
FblI GTMKAC 2 cut(s) 306, 440
Fnu4HI GCNGC 6 cut(s) 84, 109, 485, 488, 527, 576
FokI GGATG 3 cut(s) 137, 400, 585
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 6 cut(s) 84, 109, 485, 488, 527, 576
FspBI CTAG 1 cut(s) 780
GlaI GCGC 1 cut(s) 684
GluI GCNGC 6 cut(s) 84, 109, 485, 488, 527, 576
GsaI CCCAGC 1 cut(s) 45
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 3 cut(s) 65, 407, 663
HapII CCGG 5 cut(s) 97, 242, 365, 404, 444
HgaI GACGC 5 cut(s) 317, 321, 409, 524, 541
HhaI GCGC 1 cut(s) 685
Hin1I GRCGYC 6 cut(s) 303, 309, 401, 516, 533, 545
Hin1II CATG 1 cut(s) 796
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HincII GTYRAC 3 cut(s) 278, 307, 441
HindII GTYRAC 3 cut(s) 278, 307, 441
HpaII CCGG 5 cut(s) 97, 242, 365, 404, 444
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 5 cut(s) 278, 307, 346, 441, 787
Hpy188I TCNGA 4 cut(s) 116, 195, 234, 340
Hpy8I GTNNAC 5 cut(s) 278, 307, 346, 441, 787
HpyAV CCTTC 8 cut(s) 143, 426, 497, 505, 529, 546, 619, 792
HpyCH4III ACNGT 1 cut(s) 738
HpyCH4IV ACGT 5 cut(s) 303, 342, 360, 449, 545
HpyCH4V TGCA 2 cut(s) 4, 426
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 71, 80, 432, 469, 532
HpyF3I CTNAG 2 cut(s) 328, 473
HpySE526I ACGT 5 cut(s) 303, 342, 360, 449, 545
Hsp92I GRCGYC 6 cut(s) 303, 309, 401, 516, 533, 545
Hsp92II CATG 1 cut(s) 796
HspAI GCGC 1 cut(s) 683
KroI GCCGGC 1 cut(s) 403
KroNI GCCGGC 1 cut(s) 405
Kzo9I GATC 3 cut(s) 234, 283, 583
LguI GCTCTTC 2 cut(s) 425, 629
LmnI GCTCC 2 cut(s) 492, 563
Lsp1109I GCAGC 4 cut(s) 120, 471, 513, 562
LweI GCATC 1 cut(s) 378
MabI ACCWGGT 1 cut(s) 762
MaeI CTAG 1 cut(s) 780
MaeII ACGT 5 cut(s) 303, 342, 360, 449, 545
MaeIII GTNAC 1 cut(s) 619
MalI GATC 3 cut(s) 236, 285, 585
MboI GATC 3 cut(s) 234, 283, 583
MboII GAAGA 8 cut(s) 23, 208, 247, 442, 643, 646, 715, 737
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 560
MluCI AATT 1 cut(s) 101
MnlI CCTC 9 cut(s) 70, 122, 146, 202, 337, 418, 462, 468, 645
MroNI GCCGGC 1 cut(s) 403
MseI TTAA 2 cut(s) 642, 817
MslI CAYNNNNRTG 1 cut(s) 603
MspI CCGG 5 cut(s) 97, 242, 365, 404, 444
MspR9I CCNGG 3 cut(s) 26, 98, 764
MvaI CCWGG 2 cut(s) 26, 764
MvnI CGCG 3 cut(s) 264, 671, 701
MwoI GCNNNNNNNGC 6 cut(s) 51, 71, 80, 432, 469, 532
NaeI GCCGGC 1 cut(s) 405
NciI CCSGG 1 cut(s) 98
NcoI CCATGG 1 cut(s) 792
NdeII GATC 3 cut(s) 234, 283, 583
NgoMIV GCCGGC 1 cut(s) 403
NlaIII CATG 1 cut(s) 796
NmeAIII GCCGAG 2 cut(s) 41, 439
NmuCI GTSAC 1 cut(s) 619
PciSI GCTCTTC 2 cut(s) 425, 629
PcsI WCGNNNNNNNCGW 8 cut(s) 174, 297, 309, 329, 407, 446, 551, 662
PdiI GCCGGC 1 cut(s) 405
PflFI GACNNNGTC 1 cut(s) 302
PflMI CCANNNNNTGG 1 cut(s) 31
PkrI GCNGC 6 cut(s) 85, 110, 486, 489, 528, 577
PsiI TTATAA 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 560
Psp1406I AACGTT 1 cut(s) 360
Psp6I CCWGG 2 cut(s) 24, 762
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 2 cut(s) 24, 762
PspPI GGNCC 2 cut(s) 239, 662
PsuI RGATCY 1 cut(s) 283
PsyI GACNNNGTC 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 603
Rsr2I CGGWCCG 1 cut(s) 239
RsrII CGGWCCG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 560
SalI GTCGAC 2 cut(s) 305, 439
SapI GCTCTTC 2 cut(s) 425, 629
SaqAI TTAA 2 cut(s) 642, 817
SatI GCNGC 6 cut(s) 84, 109, 485, 488, 527, 576
Sau3AI GATC 3 cut(s) 234, 283, 583
Sau96I GGNCC 2 cut(s) 239, 662
ScrFI CCNGG 3 cut(s) 26, 98, 764
SduI GDGCHC 1 cut(s) 560
SexAI ACCWGGT 1 cut(s) 762
SfaNI GCATC 1 cut(s) 378
SgrDI CGTCGACG 1 cut(s) 305
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 603
Sse9I AATT 1 cut(s) 101
SsiI CCGC 9 cut(s) 81, 84, 262, 368, 463, 487, 490, 507, 671
SspMI CTAG 1 cut(s) 780
SstI GAGCTC 1 cut(s) 560
StyD4I CCNGG 3 cut(s) 24, 96, 762
StyI CCWWGG 2 cut(s) 87, 792
TaaI ACNGT 1 cut(s) 738
TaiI ACGT 5 cut(s) 306, 345, 363, 452, 548
TaqI TCGA 8 cut(s) 21, 141, 177, 306, 440, 554, 650, 677
TaqII GACCGA 1 cut(s) 227
TasI AATT 1 cut(s) 101
TauI GCSGC 2 cut(s) 86, 490
Tru1I TTAA 2 cut(s) 642, 817
Tru9I TTAA 2 cut(s) 642, 817
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 4 cut(s) 108, 484, 526, 575
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 1 cut(s) 162
Tth111I GACNNNGTC 1 cut(s) 302
Van91I CCANNNNNTGG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 1 cut(s) 101
XcmI CCANNNNNNNNNTGG 1 cut(s) 568
XmiI GTMKAC 2 cut(s) 306, 440
XspI CTAG 1 cut(s) 780
ZraI GACGTC 2 cut(s) 304, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.